a antibody  (Alomone Labs)

Journal: The American Journal of Pathology

Article Title: Vascular Dysfunction in Pneumocystis -Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration

doi: 10.1016/j.ajpath.2015.10.008

Figure Lengend Snippet: ET-1 concentrations are not significantly different in BALB-STD or IFN-γ-STD mice compared with controls. Samples are peptide extracts from total lung homogenates. Data are expressed as means ± SEM. n = 5 mice in two independent experiments. CON, control; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.

Article Snippet: Polyclonal anti-endothelin receptor A (ET A R) and the control peptide to verify specificity were from Alomone Labs (no. AER-001; Jerusalem, Israel).

Techniques:

Journal: The American Journal of Pathology

Article Title: Vascular Dysfunction in Pneumocystis -Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration

doi: 10.1016/j.ajpath.2015.10.008

Figure Lengend Snippet: Small pulmonary artery profiles show increasing vasoconstriction when treated with a cumulative dose response of ET-1. Shown is a representative profile from a BALB-STD mouse precision cut lung slices sample. ET-1, endothelin-1; STD, short-term depletion.

Article Snippet: Polyclonal anti-endothelin receptor A (ET A R) and the control peptide to verify specificity were from Alomone Labs (no. AER-001; Jerusalem, Israel).

Techniques:

Journal: The American Journal of Pathology

Article Title: Vascular Dysfunction in Pneumocystis -Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration

doi: 10.1016/j.ajpath.2015.10.008

Figure Lengend Snippet: The response to ET-1 is enhanced in BALB-STD mice. A: Vasoconstriction in small pulmonary artery profiles in BALB-STD mice is significantly greater over the range of 10−10 to 10−9 ET-1. B: This coincides with a significant increase in the ETAR in BALB-STD mice total lung tissue as indicated by quantification of Western blot analysis. Data are expressed as means ± SEM. n = 4 to 5 (B) or n = 16 to 35 profiles per group across 4 to 5 mice (A) in two independent experiments. ∗P ≤ 0.05 versus IFN-γ-STD and controls. CON, control; ETAR, endothelin receptor A; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.

Article Snippet: Polyclonal anti-endothelin receptor A (ET A R) and the control peptide to verify specificity were from Alomone Labs (no. AER-001; Jerusalem, Israel).

Techniques: Western Blot