acr 002  (Alomone Labs)

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: The endocannabinoid system regulates both ependymoglial and neuronal cell responses to a tail amputation in the axolotl.

doi: 10.1002/dvdy.70035

Figure Lengend Snippet: FIGURE 1 Identification and conservation of the axolotl endocannabinoid receptors. (A) Protein sequence alignment of the putative axolotl CB1 sequence with the rat and zebrafish CB1 sequence. (B) Protein sequence alignment of the putative axolotl CB2 sequence with the rat and zebrafish CB2 sequence. Red asterisks and red boxes indicate amino acids that are conserved between all three species. (C) Western blot using the rat CB1 antibody on axolotl tail tissue demonstrates a single prominent band at 120 kDa (n = 3). (D) Western blot using the rat CB2 antibody on axolotl tail tissue demonstrates two bands at a similar molecular weight of 46 kDa (n = 3). MW = molecular weight (for each band in ladder). (E) Preadsorption control for the CB1 antibody using either CB1 or CB2 antigenic peptides (n = 3). (F) Preadsorption control for the CB2 antibody using CB1 or CB2 antigenic peptides (n = 3).

Article Snippet: Overnight incubations in primary antibodies were then performed at 4 C using 1:1000 CB1 (Alomone Labs) and 1:1000 CB2 (Alomone Labs).

Techniques: Sequencing, Western Blot, Molecular Weight, Control

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: The endocannabinoid system regulates both ependymoglial and neuronal cell responses to a tail amputation in the axolotl.

doi: 10.1002/dvdy.70035

Figure Lengend Snippet: FIGURE 2 CB1 and CB2 are upregulated in response to tail amputation. (A) Western blot analysis demonstrates a significant upregulation of CB1 in the first 3 days after tail amputation, compared to uninjured controls (n = 3; F(4,40) = 5.994, p = .0007, one-way ANOVA). (B) No change in CB2 expression is shown in the first 3 days post tail amputation (n = 3; F(4,40) = 2.779, p = .0397, one-way ANOVA). (C) Western blot analysis demonstrates a significant upregulation of CB1 expression at both 7 and 14 days after tail amputation (n = 3; F(2,24) = 15.97, p < .0001, one-way ANOVA). (D) Western blot analysis demonstrates a significant upregulation of CB2 at 7 and 14 days post tail amputation (n = 3; F(2,24) = 10.84, p = .0004, one-way ANOVA). Uninj = uninjured tail tissue. hpa = hours post tail amputation; dpa = days post tail amputation. ns = not significant. *p < .05, **p < .01, ***p < .001, ****p < .0001 compared to uninjured controls. #p < .05.

Article Snippet: Overnight incubations in primary antibodies were then performed at 4 C using 1:1000 CB1 (Alomone Labs) and 1:1000 CB2 (Alomone Labs).

Techniques: Western Blot, Expressing

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: The endocannabinoid system regulates both ependymoglial and neuronal cell responses to a tail amputation in the axolotl.

doi: 10.1002/dvdy.70035

Figure Lengend Snippet: FIGURE 4 Inhibiting CB1 and CB2 receptor signaling impairs tail regeneration. (A) Representative images of tail regenerates after a 7-day treatment with the vehicle (control, i), 1 μM AM251 (ii), or 1 μM AM630 (iii). Black dotted line indicates the original plane of amputation. Scale bar: 1 mm. (B, C) Graphs show that the proportional increase in axolotl body length was significantly reduced following either a 7-day treatment with either 1 μM AM251 (n = 8; B) or after a 7-day treatment with 1 μM AM630 (n = 8; C) compared to the vehicle control (unpaired t tests). (D) Graph shows a significant reduction in the proportional increase in axolotl body length (7 days after tail amputation) following only a 1-day pulse treatment with either 1 μM AM251 (n = 10) or 1 μM AM630 (n = 10), compared to vehicle controls (n = 10; F(2,27) = 18.86; p < .0001, one-way ANOVA). (E) Western blot analyses show that treatment with AM251 prevented the upregulation of CB1 that normally occurs in untreated or vehicle-treated control animals at 7-days post tail amputation (n = 3; Constant 7-day bath treatment: F(3,32) = 14.69; p < .0001; 1-day pulse treatment: F(3,32) = 18.60; p < .0001; one-way ANOVAs). Representative blot for 1-day pulse treatment shown. (F) Treatment with AM630 prevented the upregulation of CB2 that normally occurs in untreated or vehicle- treated control animals at 7-days post tail amputation (n = 3; constant treatment: F(3,32) = 24.80; p < .0001; 1-day pulse treatment: F(3,32) = 11.60; p < .0001; one-way ANOVAs). Representative blot for 7-day constant treatment shown. **p < .01, ***p < .001, ****p < .0001 compared to vehicle controls. ###p < .001. ####p < .0001.

Article Snippet: Overnight incubations in primary antibodies were then performed at 4 C using 1:1000 CB1 (Alomone Labs) and 1:1000 CB2 (Alomone Labs).

Techniques: Control, Western Blot

Journal: Pharmaceuticals

Article Title: Exploring Cannabinoids with Enhanced Binding Affinity for Targeting the Expanded Endocannabinoid System: A Promising Therapeutic Strategy for Alzheimer’s Disease Treatment

doi: 10.3390/ph17040530

Figure Lengend Snippet: Antibodies, including both primary and secondary types, along with the corresponding dilutions utilized in immunohistochemical analysis.

Article Snippet: Anti-canabinoid receptor antibodies (ACR-002), Alomone Labs (Jerusalem, Israel) , 1:100 , Goat anti-rabbit , 1:100.

Techniques: Immunohistochemical staining

Journal: CNS Neuroscience & Therapeutics

Article Title: Update on the controversial identity of cells expressing cnr2 gene in the nervous system

doi: 10.1111/cns.13977

Figure Lengend Snippet: List of anti‐CB2R antibodies used for the detection of CB2R protein in the central nervous system

Article Snippet: D , #ACR‐002 , Alomone , Yes , aa 228–242 of rat CB2R , Rabbit , Poly , Mouse, rat.

Techniques:

Journal: The Journal of Headache and Pain

Article Title: Dual action of the cannabinoid receptor 1 ligand arachidonyl-2′-chloroethylamide on calcitonin gene-related peptide release

doi: 10.1186/s10194-022-01399-8

Figure Lengend Snippet: Primary and secondary antibodies used

Article Snippet: CB2 , ACR-002 , Rabbit , 1:100 , 3rd intracellular loop of rat CB2 receptor , Alomone Labs, Jerusalem, Israel.

Techniques:

Journal: The Journal of Headache and Pain

Article Title: Dual action of the cannabinoid receptor 1 ligand arachidonyl-2′-chloroethylamide on calcitonin gene-related peptide release

doi: 10.1186/s10194-022-01399-8

Figure Lengend Snippet: The expression of CB2, CGRP and RAMP1 in the TG. IHC localization of CB2, CGRP and the functional CGRP receptor (by staining for RAMP1) in the TG. Blue represents cell nuclei; orange represents colocalization of CGRP (upper panel) or RAMP1 (lower panel) with CB2; globular regions represent neuronal somas and fibrous regions represent individual neuronal fibres. Large image scale corresponds to 50 mm, and the insert scale corresponds to 10 μm. The arrows mark unmyelinated C-fibres (upper panel) and myelinated Aδ-fibres (lower panel)

Article Snippet: CB2 , ACR-002 , Rabbit , 1:100 , 3rd intracellular loop of rat CB2 receptor , Alomone Labs, Jerusalem, Israel.

Techniques: Expressing, Functional Assay, Staining