rotarod test  (Omnitech Electronics)

Journal: Acta Neuropathologica Communications

Article Title: Dual targeting of iNOS and Src tyrosine kinase as a superior therapeutic strategy against soman-induced long-term neurotoxicity: multimodal biomarker, imaging, and neurobehavioral outcome analyses

doi: 10.1186/s40478-025-02220-8

Figure Lengend Snippet: Motor behavioral assessment and its correlation with rsFC of different brain networks post-soman exposure. a A Rotarod (RR) apparatus was used to test the motor behavior of rats at 6 weeks post-soman. The time to fall for each animal is plotted as mean ± SEM in ( b ) and as difference in mean compared to control in ( c ), depicted as a horizontal line. * indicates the soman effect (soman vs control) and # represents treatment effect (vs soman + vehicle). d , e A correlation matrix depicting a correlation coefficient (Pearson correlation, p < 0.05) between time to fall (seconds) in RR (6 weeks post soman) and rsFC of different regional networks (8–9 weeks post soman) is presented, with ‘X’ marks p -value ≥ 0.05 (not significant). f – i Simple linear regression comparing between the time to fall (seconds) and the rsFC (active voxels) with the corresponding correlation coefficient (r) and p -value. Each dot on the graphs represents a data point from each animal. n = 15–30 (7–16/sex), * indicates soman effect (vs control) and # represents effect (vs Soman + vehicle) with corresponding p -values

Article Snippet: Motor learning and coordination in rodents were assessed using an accelerating rotarod apparatus (AccuRotor 4-channel, Omnitech Electronics Inc., and Start Fusion v6.2 AccuRotor Edition Software) as previously described [ ].

Techniques: Control

Journal: Journal of Neurochemistry

Article Title: A Longitudinal Study of Sex Differences in a TDP ‐43 Mouse Model Reveals STI1 Regulation of TDP ‐43 Proteinopathy and Motor Deficits

doi: 10.1111/jnc.70204

Figure Lengend Snippet: TAR4 mice show sex‐specific motor deficits. (a) Longitudinal timeline for hemizygous TAR4 mice. (b) At each timepoint mice were analyzed on rotarod, wire hang, catwalk gait analysis, hindlimb clasping, open field, and weight tracking. (c, d) Rotarod test of TAR4 and wild‐type male and female mice. The average amount of time spent on the rod is shown across four time points. (e, f) Wire hang task of male and female TAR4 and wild‐type mice. (g, h) Stride length in millimeters measured by catwalk gait analysis test for male and female TAR4 and wild‐type mice. (i, j) Swing time measured in seconds (s) by catwalk gait analysis test of male and female TAR4 and wild‐type mice. (k, l) BOS measurement in centimeters (cm) is shown for male TAR4 and wild‐type mice of front and hindlimbs. (m, n) Female TAR4 and wild‐type BOS. Mixed‐effects model with multiple comparisons was used to analyze differences within groups (black lines) and between groups (asterisks above time points). All data are represented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Numbers represent mice tested in each timepoint.

Article Snippet: Rotarod was performed using AccuRotor Four Animal Rotarod (Omnitech Electronics Inc).

Techniques:

Journal: Journal of Neurochemistry

Article Title: A Longitudinal Study of Sex Differences in a TDP ‐43 Mouse Model Reveals STI1 Regulation of TDP ‐43 Proteinopathy and Motor Deficits

doi: 10.1111/jnc.70204

Figure Lengend Snippet: Reducing cytoplasmic TDP‐43 levels in TAR4 mice does not rescue motor deficits in males. (a, b) Rotarod performance as measured by latency to fall in seconds (s) is shown for TAR4, TAR4‐STI1 −/+ , and TAR4‐STI1 TGA males and females over time, respectively. (c, d) Wire hang performance as measured by latency to fall in seconds (s) is shown for TAR4, TAR4‐STI1 −/+ , and TAR4‐STI1 TGA males and females over time, respectively. (e, f) Hindlimb clasping scores are shown at final time point of 15–17 months of age for males and females, respectively. (g, h) Probability of survival is shown for TAR4, TAR4‐STI1 −/+ , and TAR4‐STI1 TGA males and females, respectively. Mixed‐effects model with multiple comparisons was used to analyze datasets (a–d); differences within groups are represented as black lines and between groups as asterisks above time points. Ordinary one‐way ANOVA with multiple comparisons was used for analysis of datasets (e, f). Simple survival analysis (Kaplan–Meier) with multiple comparisons was used for analysis of (g, h) analysis. All data are represented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Numbers represent number of mice at each timepoint.

Article Snippet: Rotarod was performed using AccuRotor Four Animal Rotarod (Omnitech Electronics Inc).

Techniques:

Journal: Journal of Neurochemistry

Article Title: A Longitudinal Study of Sex Differences in a TDP ‐43 Mouse Model Reveals STI1 Regulation of TDP ‐43 Proteinopathy and Motor Deficits

doi: 10.1111/jnc.70204

Figure Lengend Snippet: Hypomorphic STI1 allele is sufficient to correct some motor deficits in TAR4 males. (a, b) Western blot of cytoplasmic TDP‐43 proteins from TAR4 and TAR4‐ΔHET male cortices quantified relative to amido black. (c, d) Western blot of urea‐soluble TDP‐43 levels from TAR4 and TAR4‐ΔHET male cortices quantified relative to amido black. (e) Rotarod performance as measured by latency to fall is shown for TAR4 and TAR4‐ΔHET males. (f) Wire hang performance as measured by latency to fall is shown for TAR4 and TAR4‐ΔHET males. (g–j) Catwalk gait analysis performance for TAR4 and TAR4‐ΔHET mice showing swing time (s), stride length, front BOS, and hind BOS. (k) Stride length for each paw of TAR4 and TAR4‐ΔHET mice at 15–17 months of age. (l) Hindlimb clasping reflex scores for TAR4 and TAR4‐ΔHET mice. Unpaired t‐tests were used for (a–d) analysis ( n = 5 mice/group). Mixed‐effects model with multiple comparisons was used to analyze datasets (e–j); differences within groups are represented as black lines and between groups as asterisks above time points. Two‐way ANOVA with multiple comparisons was used for (k) analysis. Unpaired t ‐test was used for analysis of dataset (l). All data are represented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Numbers represent number of mice at each timepoint.

Article Snippet: Rotarod was performed using AccuRotor Four Animal Rotarod (Omnitech Electronics Inc).

Techniques: Western Blot

Journal: bioRxiv

Article Title: Exoproteome of calorie-restricted humans identifies complement deactivation as an immunometabolic checkpoint reducing inflammaging

doi: 10.1101/2025.08.04.668533

Figure Lengend Snippet: (a-q) Aged (20-month-old) male mice were randomly allocated and intraperitoneally injected with either vehicle or the C3 inhibitor (AMY-101; 1 mg/kg) weekly for 6 weeks and analyzed ( n = 9 for vehicle and n = 8 for C3 inhibitor). (a) Schematic diagram for the experimental design. (b) Body weight measurement. (c,d) Fat mass (c) and lean mass (d) were measured by EchoMRI. (e) Representative western blots with densitometry ( n = 4 for vehicle and n = 5 for C3 inhibitor). (f) A glucose tolerance test was performed. (g,h) Healthspan was analyzed through grip strength (g) and the rotarod test (h). (i-m) Serum levels of IL-1β (i), IL-6 (j), TNF-α (k), MCP-1 (l), and GM-CSF (m) were analyzed by Luminex assay. Only detected values were presented. (n-q) Flow cytometry analysis of VAT SVF. The frequencies of ATMs (n), AABs (o), CD8 T (p), and NK cells (q) were presented. (r-t) Representative C3 western blot analysis of the VAT of 24-month-old WT and FGF21-Tg (r), 24-month-old WT and PLA2G7 -/- (s), and 16-month-old SPARC f/f and SPARC f/f;CAG-Cre (t) mice ( n = 3/group). Unpaired two-tailed t-tests were performed, and exact p-values were presented. Data presented as mean±SEM.

Article Snippet: A rotarod test was conducted using an accurotor rotarod (AccuScan Instruments).

Techniques: Injection, Western Blot, Luminex, Flow Cytometry, Two Tailed Test

Journal: bioRxiv

Article Title: Exoproteome of calorie-restricted humans identifies complement deactivation as an immunometabolic checkpoint reducing inflammaging

doi: 10.1101/2025.08.04.668533

Figure Lengend Snippet: (a-q) Aged (20-month-old) male mice were randomly allocated and intraperitoneally injected with either vehicle or the C3 inhibitor (AMY-101; 1 mg/kg) weekly for 6 weeks and analyzed ( n = 9 for vehicle and n = 8 for C3 inhibitor). (a) Schematic diagram for the experimental design. (b) Body weight measurement. (c,d) Fat mass (c) and lean mass (d) were measured by EchoMRI. (e) Representative western blots with densitometry ( n = 4 for vehicle and n = 5 for C3 inhibitor). (f) A glucose tolerance test was performed. (g,h) Healthspan was analyzed through grip strength (g) and the rotarod test (h). (i-m) Serum levels of IL-1β (i), IL-6 (j), TNF-α (k), MCP-1 (l), and GM-CSF (m) were analyzed by Luminex assay. Only detected values were presented. (n-q) Flow cytometry analysis of VAT SVF. The frequencies of ATMs (n), AABs (o), CD8 T (p), and NK cells (q) were presented. (r-t) Representative C3 western blot analysis of the VAT of 24-month-old WT and FGF21-Tg (r), 24-month-old WT and PLA2G7 -/- (s), and 16-month-old SPARC f/f and SPARC f/f;CAG-Cre (t) mice ( n = 3/group). Unpaired two-tailed t-tests were performed, and exact p-values were presented. Data presented as mean±SEM.

Article Snippet: A rotarod test was conducted using an accurotor rotarod (AccuScan Instruments).

Techniques: Injection, Western Blot, Luminex, Flow Cytometry, Two Tailed Test