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alpha 1c subunit vdcc  (Alomone Labs)


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    Structured Review

    Alomone Labs alpha 1c subunit vdcc
    Representative blots are shown for <t>VDCC</t> (A). The amount of the <t>alpha-1c</t> subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).
    Alpha 1c Subunit Vdcc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acc-003-ag/Anti-Cav1%2E2+(CACNA1C)-ATTO+Fluor-488+Antibody/pmc03923070-22-3-11
    Average 90 stars, based on 2 article reviews
    alpha 1c subunit vdcc - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Attenuation of L-Type Ca 2+ Channel Expression and Vasomotor Response in the Aorta with Age in Both Wistar-Kyoto and Spontaneously Hypertensive Rats"

    Article Title: Attenuation of L-Type Ca 2+ Channel Expression and Vasomotor Response in the Aorta with Age in Both Wistar-Kyoto and Spontaneously Hypertensive Rats

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0088975

    Representative blots are shown for VDCC (A). The amount of the alpha-1c subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).
    Figure Legend Snippet: Representative blots are shown for VDCC (A). The amount of the alpha-1c subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).

    Techniques Used:

    Related Articles

    Blocking Assay:

    Article Title: Nimodipine fosters remyelination in a mouse model of multiple sclerosis and induces microglia-specific apoptosis
    Article Snippet: .. Because the antibody (diluted 1:400 in blocking solution) (Alomone Labs; catalog no. ACC-003-AG) was directly conjugated with ATTO-488, no secondary antibody was needed. .. Goat anti-rabbit FITC-conjugated IgG (Jackson ImmunoResearch; catalog no. 111-095-003) was used as background control.

    Article Title: CaV1.2 and CaV1.3 voltage-gated L-type Ca2+ channels in rat white fat adipocytes
    Article Snippet: .. Cells were permeablised in blocking buffer (PBS with 3% BSA and 0.5% Triton-100x) for 10 min then stained with primary anti-Calcium Channel L-type alpha 1C subunit (Cacna1C) antibody conjugated with Atto 594 (ACC-003-AG, Alomone) at 1:200 dilution for 16 h (Raifman et al. 2017). ..

    other:

    Article Title: Modulation of TRPM8 alters the phagocytic activity of microglia and induces changes in sub-cellular organelle functions.
    Article Snippet: In this work, we investigated the presence and function of TRPM8, a non-selective and cold-sensitive Ca2+permeable ion channel in the primary microglia cell as well as in microglia cell line BV2.. We demonstrate that primary microglia as well as BV2 express TRPM8 endogenously.. Both pharmacological activation or inhibition of TRPM8 causes enhanced uptake of bacterial particles at early time points of infection.

    Article Title: Genetic, Cellular, and Functional Evidence for Ca2+ Inflow through Cav1.2 and Cav1.3 Channels in Murine Spiral Ganglion Neurons
    Article Snippet: The samples were incubated for 1 h in a blocking solution containing PBS, 1% horse serum in PBS, followed by 3–5 h incubation with Ca 2 channel antibodies against Cav1.2, residues 865– 881, and Cav1.3, residues 859 – 875 (Alomone Labs; Abcam), at 1:100 to 1:500 dilutions.

    Incubation:

    Article Title: Protein kinase C enhances plasma membrane expression of cardiac L-type calcium channel, Ca V 1.2
    Article Snippet: Cells were blocked in 10% normal donkey serum (Jackson Immunoresearch Laboratories) to eliminate non-specific binding. .. Cells were then incubated with anti-Ca V 1.2-ATTO-488 antibody (Alomone Labs) overnight at 4°C, rinsed in PBS to remove excess antibody and images were taken with a confocal laser scanning microscope (Zeiss 510 META). ..

    Laser-Scanning Microscopy:

    Article Title: Protein kinase C enhances plasma membrane expression of cardiac L-type calcium channel, Ca V 1.2
    Article Snippet: Cells were blocked in 10% normal donkey serum (Jackson Immunoresearch Laboratories) to eliminate non-specific binding. .. Cells were then incubated with anti-Ca V 1.2-ATTO-488 antibody (Alomone Labs) overnight at 4°C, rinsed in PBS to remove excess antibody and images were taken with a confocal laser scanning microscope (Zeiss 510 META). ..

    Staining:

    Article Title: CaV1.2 and CaV1.3 voltage-gated L-type Ca2+ channels in rat white fat adipocytes
    Article Snippet: .. Cells were permeablised in blocking buffer (PBS with 3% BSA and 0.5% Triton-100x) for 10 min then stained with primary anti-Calcium Channel L-type alpha 1C subunit (Cacna1C) antibody conjugated with Atto 594 (ACC-003-AG, Alomone) at 1:200 dilution for 16 h (Raifman et al. 2017). ..



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    Representative blots are shown for <t>VDCC</t> (A). The amount of the <t>alpha-1c</t> subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).
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    Representative blots are shown for <t>VDCC</t> (A). The amount of the <t>alpha-1c</t> subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).
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    Representative blots are shown for <t>VDCC</t> (A). The amount of the <t>alpha-1c</t> subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).
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    Representative blots are shown for <t>VDCC</t> (A). The amount of the <t>alpha-1c</t> subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).
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    Representative blots are shown for <t>VDCC</t> (A). The amount of the <t>alpha-1c</t> subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).
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    Representative blots are shown for <t>VDCC</t> (A). The amount of the <t>alpha-1c</t> subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).
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    Representative blots are shown for <t>VDCC</t> (A). The amount of the <t>alpha-1c</t> subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).
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    Alomone Labs l type channels cav1 2
    Representative blots are shown for <t>VDCC</t> (A). The amount of the <t>alpha-1c</t> subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).
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    Image Search Results


    Representative blots are shown for VDCC (A). The amount of the alpha-1c subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).

    Journal: PLoS ONE

    Article Title: Attenuation of L-Type Ca 2+ Channel Expression and Vasomotor Response in the Aorta with Age in Both Wistar-Kyoto and Spontaneously Hypertensive Rats

    doi: 10.1371/journal.pone.0088975

    Figure Lengend Snippet: Representative blots are shown for VDCC (A). The amount of the alpha-1c subunit of VDCC was determined as the ratio of VDCC to β-actin (B). Results are expressed as the mean ± SEM (n = 6). ** P <0.01 vs each 8-week rat strain. Confocal measurement of VDCC (C) was performed in rat aortic segments (14 µm).

    Article Snippet: Rabbit anti-Cav1.2, the alpha-1c subunit VDCC, primary antibody was obtained from Alomone Labs (Jerusalem, Israel).

    Techniques: