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oct4  (Danaher Inc)


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    Structured Review

    Danaher Inc oct4
    Oct4, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 644 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab9797/Recombinant+rat+GM-CSF+protein/pmc06511878-124-25-26
    Average 99 stars, based on 644 article reviews
    oct4 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Comparison of two protocols for the generation of iPSC-derived human astrocytes
    Article Snippet: .. Primary antibodies were incubated overnight at four degree at the given dilutions: Oct4 (Abcam, ab19857, 1:1000), TRA-1–60 (Merck Millipore, MAB4360, 1:1000), Sox2 (Santa Cruz Biotechnology, sc-365823, 1:1000), Nestin (Novus, MAB1259, 1:1000), Musashi1 (Abcam, ab21628, 1:500), Pax6 (Imtec Diagnostics, 901,301, 1:1000), Sox1 (R&D Systems, AF3369, 1:100), GFAP (Dako, z0334, 1:500), Vimentin (Abcam, ab24525, 1:500). ..

    Article Title: NSUN5 is essential for proper Hippo signaling in mouse preimplantation development
    Article Snippet: .. After washing three times in PBS containing 0.3% polyvinylpyrrolidone (K-30; Nacalai Tesque), fixed embryos were treated with 0.5% Triton X-100 (Sigma-Aldrich) in PBS at room temperature for 40 min, and were blocked in PBST containing 1.5% BSA, 0.2% sodium azide, and 0.02% Tween 20 (antibody dilution buffer) at room temperature for 1 h. Embryos were incubated with one of the following primary antibodies in antibody dilution buffer at 4°C overnight: anti-YAP1 (1:2,000 dilution; DBH1X; Cell Signaling, Danvers, MA), anti-CDX2 (1:100 dilution; MU392A-UC; Biogenex, Fremont, CA), and anti-OCT4 (1:200 dilution; ab19857; Abcam, Cambridge, UK). .. Embryos were washed three times in antibody dilution buffer and then incubated with an appropriate secondary antibody in antibody dilution buffer (1:500 dilution; Alexa Fluor 488-conjugated goat anti-rabbit IgG or Alexa Fluor 594-conjugated goat anti-mouse IgG; Invitrogen) at room temperature for 1 h. After staining with Hoechst 33342 (Sigma-Aldrich), embryos were mounted on slides in Vectashield mounting medium (Vector Laboratories, Newark, CA) and signals were observed using a fluorescence microscope (BX53 or IX73; Olympus, Tokyo, Japan).

    other:

    Article Title: Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 gene
    Article Snippet: , Rabbit anti-OCT3/4 , 1:200 , Abcam, cat# ab19857 , AB_445175.


    Immunostaining:

    Article Title: Hallmark molecular and pathological features of POLG disease are recapitulated in cerebral organoids
    Article Snippet: Mounted sections were incubated for 1 hour at room temperature with and blocked using blocking buffer containing (Sigma-Aldrich, #G9023) and 0.1% (v/v) Triton X100 (Sigma-Aldrich, #9036-19-5), and then incubated with primary antibodies diluted in blocking solution overnight at 4°C. .. The following primary antibodies were used for immunostaining: Nanog (rabbit, 1:100; Abcam, #ab80892), Oct4 (rabbit, 1:100; Abcam, #ab19857), SSEA4 (mouse, 1:200; Abcam, #ab16287,), NeuN (rabbit, 1:500; Cell Signaling, #24307S), MAP2 (rabbit, 1:1000; Abcam, #ab5392), β-tubulin III (TUJ1) (mouse, 1:1000; Abcam, #ab78078), Synaptophysin (mouse, 1:500; Proteintech, #17785-1-AP), PSD-95 (rabbit, 1:500; Proteintech, #20665-1-AP), SOX2 (rabbit, 1:100; Abcam, #ab97959), NDUFB10 (rabbit, 1:350; Abcam 196019), TFAM (mouse, 1:1000; Abcam #ab119684), TOMM20 (mouse, 1:350; Abcam, #ab56783), GFAP (chicken, 1:500; Abcam #ab4674), DCX (mouse, 1:200; Abcam #ab135349), SATB2 (rabbit, 1:400; Abcam #ab4674), CTIP2 (rat, 1:500; Abcam, #ab18465), OLIG2 (rabbit, 1:500; Abcam, #ab42453). .. Alexa Fluor Dyes (Invitrogen, #A11008, #A21449, #A11012, #A21141, #A11042, #A21236) were used at 1:800 dilution as secondary antibodies.

    Expressing:

    Article Title: Early nuclear phenotypes and reactive transformation in human iPSC-derived astrocytes from ALS patients with SOD1 mutations
    Article Snippet: .. Undifferentiated state of iPSCs was assessed by testing for expression of the stem cell markers NANOG and OCT4 using rabbit anti-NANOG (1/1,000; Abcam; Cambridge, UK; Cat. No. ab21624) and rabbit anti-OCT4 (1 μg/ml; Abcam; Cat. No. ab19857) or goat anti-OCT3/4 (1/500; Santa Cruz Biotechnology; Dallas, TX, USA, Cat. No. sc-8628) antibodies, and by quality control profiling as described previously ( ). .. Human iPSCs at low passage number were cultured in mTeSR medium (STEMCELL Technologies; Vancouver, BC, Canada; Cat. No. 85850) in 10-cm culture dishes (Thermo-Fisher Scientific; Waltham, MA; Cat. No. 353003) coated with Matrigel (Thermo-Fisher Scientific; Cat. No. 08-774-552) until they reached 70%-80% confluence.

    Control:

    Article Title: Early nuclear phenotypes and reactive transformation in human iPSC-derived astrocytes from ALS patients with SOD1 mutations
    Article Snippet: .. Undifferentiated state of iPSCs was assessed by testing for expression of the stem cell markers NANOG and OCT4 using rabbit anti-NANOG (1/1,000; Abcam; Cambridge, UK; Cat. No. ab21624) and rabbit anti-OCT4 (1 μg/ml; Abcam; Cat. No. ab19857) or goat anti-OCT3/4 (1/500; Santa Cruz Biotechnology; Dallas, TX, USA, Cat. No. sc-8628) antibodies, and by quality control profiling as described previously ( ). .. Human iPSCs at low passage number were cultured in mTeSR medium (STEMCELL Technologies; Vancouver, BC, Canada; Cat. No. 85850) in 10-cm culture dishes (Thermo-Fisher Scientific; Waltham, MA; Cat. No. 353003) coated with Matrigel (Thermo-Fisher Scientific; Cat. No. 08-774-552) until they reached 70%-80% confluence.



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