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anti phospho ampk  (Danaher Inc)


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    Structured Review

    Danaher Inc anti phospho ampk
    Anti Phospho Ampk, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 2105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab9797/Nitrocellulose+Transfer+Membrane-+-+10Membrane/pmc05161403-36-5-37
    Average 99 stars, based on 2105 article reviews
    anti phospho ampk - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models
    Article Snippet: .. The pharmacological agents used were 5 or 10 μ m CCCP (Merck, C2759) for 6 h or 24 h (also 1.25, 2.5, 5, 10, 20 and 40 μ m for dose-response treatments), and 100 n m MLi-2 (Tocris, 5756) for 48 h. The primary antibodies used for western blotting (WB) or for immunocytochemistry (ICC) were rabbit anti-LRRK2 (WB, 1:100; Abcam ab133474), rabbit anti-LRRK2 (phospho S1292; WB, 1:100; Abcam ab203181) mouse anti-MIRO1 (WB, 1:700; Merck, WH0055288M1), rabbit anti-RAB10 (WB, 1:1000; Cell Signaling Technology, 8127S), rabbit anti-RAB10 (phospho T73; WB, 1:250; Abcam, ab230261), rabbit anti-RAB12 (WB, 1:1000; Proteintech, 18 843-1-AP), rabbit anti-RAB12 (phospho S106; WB, 1:250; Abcam, ab256487), rabbit anti-RAB8A (phospho T72; WB, 1:100; Abcam, ab230260), rabbit anti-GFP (WB, 1:100; ThermoScientific A-11122), HRP-conjugated anti-β-actin (WB, 1:50 000, Biolegend, 643807), anti-phospho-ubiquitin Alexa Fluor-488 conjugate (phosphor Ser65; ICC, 1:500; Merck, ABS1513-I-AF488), mouse anti-TOM20 (ICC, 1:500; Santa Cruz Biotechnology, sc-17 764F-10), rat anti-TOMM20 (ICC, 1:250; Abcam, ab289670 EPR15581-39), chicken anti-MAP2 (ICC, 1:1000; Abcam, ab92434), rabbit anti-TH (ICC, 1:500; Millipore, AB152), rabbit anti-MIRO1 (ICC, 1:100; Merck, HPA010687) and rabbit anti-LRRK2 (ICC, 1:100; Antibodies Inc., 75-253S). .. Peroxidase-linked secondary antibodies used for WB were goat anti-mouse IgG (H + L)–HRP conjugate (1706516) and goat anti-rabbit IgG (H + L)–HRP conjugate (1706515) (BioRad).

    Article Title: Systematic compositional analysis of exosomal extracellular vesicles produced by cells undergoing apoptosis, necroptosis and ferroptosis
    Article Snippet: ML162 (Aobious, Gloucester, MA) was used at 20 μM. .. The following antibodies were used for immunoblot: Alix (ab76608, Abcam), TSG101 (C‐2, sc‐7964, Santa Cruz), CD63 (EPR21151, ab217345, Abcam), CD81 (EPR4244, ab109201, Abcam & D5O2Q, 10037, Cell Signalling), Flotillin‐1 (EPR6041, ab133497, Abcam), Histone H3b (D1H2, Cell Signalling), Calnexin (610523, BD Biosciences), Calreticulin (EPR3924, ab92516, Abcam), Bcl‐2‐HRP (C‐2, sc‐7382, Santa Cruz), LAMP1 (L1418, Sigma‐Aldrich), phospho‐RIPK3 (E7S1R, 91702S, Cell Signalling), RIPK3 (2283, ProSci Incorporated), phospho‐MLKL (D6E3G, 37333S, Cell Signalling), MLKL (3H1, MABC604, Millipore), cleaved Caspase‐8 (9429, Cell Signalling), Caspase‐3 (9662, Cell Signalling), Caspase‐7 (9492, Cell Signalling), PARP (46D11, 9532S, Cell Signalling) and cleaved PARP (9544S, Cell Signalling). .. HRP‐conjugated secondary antibodies were anti‐rabbit IgG, anti‐mouse IgG, or anti‐rat IgG (GE Healthcare, NA934, NA931, NA935, respectively, 1:5000).

    Immunocytochemistry:

    Article Title: Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models
    Article Snippet: .. The pharmacological agents used were 5 or 10 μ m CCCP (Merck, C2759) for 6 h or 24 h (also 1.25, 2.5, 5, 10, 20 and 40 μ m for dose-response treatments), and 100 n m MLi-2 (Tocris, 5756) for 48 h. The primary antibodies used for western blotting (WB) or for immunocytochemistry (ICC) were rabbit anti-LRRK2 (WB, 1:100; Abcam ab133474), rabbit anti-LRRK2 (phospho S1292; WB, 1:100; Abcam ab203181) mouse anti-MIRO1 (WB, 1:700; Merck, WH0055288M1), rabbit anti-RAB10 (WB, 1:1000; Cell Signaling Technology, 8127S), rabbit anti-RAB10 (phospho T73; WB, 1:250; Abcam, ab230261), rabbit anti-RAB12 (WB, 1:1000; Proteintech, 18 843-1-AP), rabbit anti-RAB12 (phospho S106; WB, 1:250; Abcam, ab256487), rabbit anti-RAB8A (phospho T72; WB, 1:100; Abcam, ab230260), rabbit anti-GFP (WB, 1:100; ThermoScientific A-11122), HRP-conjugated anti-β-actin (WB, 1:50 000, Biolegend, 643807), anti-phospho-ubiquitin Alexa Fluor-488 conjugate (phosphor Ser65; ICC, 1:500; Merck, ABS1513-I-AF488), mouse anti-TOM20 (ICC, 1:500; Santa Cruz Biotechnology, sc-17 764F-10), rat anti-TOMM20 (ICC, 1:250; Abcam, ab289670 EPR15581-39), chicken anti-MAP2 (ICC, 1:1000; Abcam, ab92434), rabbit anti-TH (ICC, 1:500; Millipore, AB152), rabbit anti-MIRO1 (ICC, 1:100; Merck, HPA010687) and rabbit anti-LRRK2 (ICC, 1:100; Antibodies Inc., 75-253S). .. Peroxidase-linked secondary antibodies used for WB were goat anti-mouse IgG (H + L)–HRP conjugate (1706516) and goat anti-rabbit IgG (H + L)–HRP conjugate (1706515) (BioRad).

    Membrane:

    Article Title: Interleukin 31 receptor α promotes smooth muscle cell contraction and airway hyperresponsiveness in asthma
    Article Snippet: .. After SDS–PAGE separation, proteins were transferred to nitrocellulose membrane and incubated overnight with primary antibodies against CHRM3 (1:1000, Abcam), phosphoMLC (1:1000, Cell Signaling Technology), totalMLC (1:1000, Cell Signaling Technology), hIL-31RA (1:100, R&D Systems), ITGB1 (1:1000, Cell Signaling Technology) and GAPDH (1:2000, Bethyl Laboratories) and followed by detection with HRP conjugated secondary antibodies. .. Proteins were quantified by normalizing to GAPDH levels using the volume integration function of the BIORAD imager software.

    Article Title: Iron oxide nanoparticles induce ferroptosis via the autophagic pathway by synergistic bundling with paclitaxel
    Article Snippet: The pretreated NCI-H446 cells and M059K cells were separately collected to extract the protein from the cell lysate following 15 min of centrifugation at 21,912 × g at 4°C, and the protein concentration was determined using a BCA protein content detection kit (cat. no. KGA902; Nanjing KeyGen Biotech Co., Ltd.). .. First, identical amounts of protein (20 μg/lane) were separated on 10% gels using SDS-PAGE, transferred to PVDF membranes and blocked with fresh 5% nonfat dry milk at room temperature for 2 h. The membrane was incubated with diluted primary antibodies, including rabbit anti-P62 (1:1,000; cat. no. ab91526; Abcam), Nrf2 (1:5,000; cat. no. ab62352; Abcam), GPX4 (1:2,000; cat. no. ab123066; Abcam), mammalian target of rapamycin complex 1 (mTORC1) (1:2,000; cat. no. ab40768; Abcam), LC3II/I (1:2,000; cat. no. ab192890; Abcam), Beclin1 (1:1,000; cat. no. ab62557; Abcam), HDAC6 (1:10,000; cat. no. ab133493; Abcam), and GAPDH (1:5,000; cat. no. KGAA002; Nanjing KeyGen Biotech Co., Ltd.). .. After washing with Tris-buffered saline-Tween (TBST) three times, the membrane was subsequently incubated with the Goat Anti-Rabbit IgG/HRP antibody (1:10,000; cat. no. KGAA002; Nanjing KeyGen Biotech Co., Ltd.) for 1 h at room temperature.

    Incubation:

    Article Title: Interleukin 31 receptor α promotes smooth muscle cell contraction and airway hyperresponsiveness in asthma
    Article Snippet: .. After SDS–PAGE separation, proteins were transferred to nitrocellulose membrane and incubated overnight with primary antibodies against CHRM3 (1:1000, Abcam), phosphoMLC (1:1000, Cell Signaling Technology), totalMLC (1:1000, Cell Signaling Technology), hIL-31RA (1:100, R&D Systems), ITGB1 (1:1000, Cell Signaling Technology) and GAPDH (1:2000, Bethyl Laboratories) and followed by detection with HRP conjugated secondary antibodies. .. Proteins were quantified by normalizing to GAPDH levels using the volume integration function of the BIORAD imager software.

    Article Title: Iron oxide nanoparticles induce ferroptosis via the autophagic pathway by synergistic bundling with paclitaxel
    Article Snippet: The pretreated NCI-H446 cells and M059K cells were separately collected to extract the protein from the cell lysate following 15 min of centrifugation at 21,912 × g at 4°C, and the protein concentration was determined using a BCA protein content detection kit (cat. no. KGA902; Nanjing KeyGen Biotech Co., Ltd.). .. First, identical amounts of protein (20 μg/lane) were separated on 10% gels using SDS-PAGE, transferred to PVDF membranes and blocked with fresh 5% nonfat dry milk at room temperature for 2 h. The membrane was incubated with diluted primary antibodies, including rabbit anti-P62 (1:1,000; cat. no. ab91526; Abcam), Nrf2 (1:5,000; cat. no. ab62352; Abcam), GPX4 (1:2,000; cat. no. ab123066; Abcam), mammalian target of rapamycin complex 1 (mTORC1) (1:2,000; cat. no. ab40768; Abcam), LC3II/I (1:2,000; cat. no. ab192890; Abcam), Beclin1 (1:1,000; cat. no. ab62557; Abcam), HDAC6 (1:10,000; cat. no. ab133493; Abcam), and GAPDH (1:5,000; cat. no. KGAA002; Nanjing KeyGen Biotech Co., Ltd.). .. After washing with Tris-buffered saline-Tween (TBST) three times, the membrane was subsequently incubated with the Goat Anti-Rabbit IgG/HRP antibody (1:10,000; cat. no. KGAA002; Nanjing KeyGen Biotech Co., Ltd.) for 1 h at room temperature.

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