Fluorescence:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Incubation:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Immunofluorescence:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Multiplex Assay:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Staining:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Mouse Assay:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Microscopy:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Proximity Ligation Assay:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Modification:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Cell Culture:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Laser-Scanning Microscopy:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
Immunohistochemistry:Article Title: Electroacupuncture relieves joint pain by weakening the enhanced neuronal CXCR2-TRPA1 signaling in peripheral sensory neurons of a mouse model of acute gouty arthritis.
Article Snippet: , #ab9797, GeneTex, USA), mouse anti-GFAP (1:500, #C9205, Merck, USA), mouse anti-IBA1 (1:500, #011-27991, Wako, Japan), rabbit anti- c-Fos (1:200, #2250, CST, USA), mouse anti-NeuN (1:500, #ab104224, Abcam, UK) and mouse anti-GS (1:500, #GTX630654, GeneTex, USA). The following day, the sections were rinsed with PBS (6 × 10 minutes) and incubated for 1 hour with a mixture of corresponding secondary
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