hoechst 33258 (Danaher Inc)
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Hoechst 33258, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab91335/Cell+Counting+Kit+8+(WST-8+%2F+CCK8)+-+1000test/pmc07933812-70-0-4
Average 99 stars, based on 490 article reviews
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Labeling:Article Title: Ferroptosis inhibition protects vascular endothelial cells and maintains integrity of the blood-spinal cord barrier after spinal cord injury Article Snippet: Samples were incubated with the following secondary antibodies for 1 hour at room temperature: Alexa Fluor 555-labeled goat anti-mouse IgG (H+L) (1:500; Abcam, Cambridge, MA, USA, Cat# ab150118, RRID: AB_2714033) and Alexa Fluor 488-labeled goat anti-rabbit IgG (H+L) (1:500; Beyotime Biotechnology, Shanghai, China, Cat# A0423; RRID: AB_2891323). .. Nuclei were labeled using 4′,6-diamine-2-benzene (DAPI) (Abcam, Cat# ab104139) or Staining:Article Title: Fidgetin interacting with microtubule end binding protein EB3 affects axonal regrowth in spinal cord injury Article Snippet: Next, corresponding secondary antibodies were applied at 20–24°C for 2 hours (Alexa Flour 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L), 1:800, Jackson ImmunoResearch Laboratories, West Grove, PA, USA, Cat# 115-545-003, RRID: AB_2338840; Alexa Flour 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1:800, Jackson ImmunoResearch Laboratories, Cat# 111-545-003, RRID:AB_2338046; Alexa Flour Cy3-conjugated AffiniPure Goat Anti-Mouse IgG(H+L), 1:800, Jackson ImmunoResearch Laboratories, Cat# 115-165-003, RRID: AB_2338680; Alexa Flour Cy3-conjugated AffiniPure Goat Anti-Rat IgG(H+L), 1:800, Jackson ImmunoResearch Laboratories, Cat# 112-165-003, RRID: AB_2338240; Alexa Flour Cy3-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1:800, Jackson ImmunoResearch Laboratories, Cat# 111-165-003, RRID: AB_2338240; and Alexa Fluor 647 AffiniPure Goat Anti-Rabbit IgG (H+L), 1:800, Jackson ImmunoResearch Laboratories, Cat# 111-605-003, RRID: AB_2338072). .. Nuclei were stained with Growth Inhibition Assay:Article Title: Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor Article Snippet: .. Cell growth inhibition assay was assessed with Serial Dilution:Article Title: Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor Article Snippet: .. Cell growth inhibition assay was assessed with Microscopy:Article Title: Astragaloside IV restrains pyroptosis and fibrotic development of pulmonary artery smooth muscle cells to ameliorate pulmonary artery hypertension through the PHD2/HIF1α signaling pathway Article Snippet: The sections were firstly permeabilized with 0.1% Triton X-100 for 30 min, and blocked with 5% bovine serum albumin for 1 h. Then, the sections were incubated overnight with primary antibody against NLRP3 (Abcam, Cambridge, UK, #ab263899), PHD2 (Abcam, #ab226890) and HIF1α (Abcam, #ab179483) at 4 °C, and incubated with Alexa Fluor®488 labeled IgG secondary antibody (Abcam, #ab150077). .. The nuclei were counterstained with Software:Article Title: Astragaloside IV restrains pyroptosis and fibrotic development of pulmonary artery smooth muscle cells to ameliorate pulmonary artery hypertension through the PHD2/HIF1α signaling pathway Article Snippet: The sections were firstly permeabilized with 0.1% Triton X-100 for 30 min, and blocked with 5% bovine serum albumin for 1 h. Then, the sections were incubated overnight with primary antibody against NLRP3 (Abcam, Cambridge, UK, #ab263899), PHD2 (Abcam, #ab226890) and HIF1α (Abcam, #ab179483) at 4 °C, and incubated with Alexa Fluor®488 labeled IgG secondary antibody (Abcam, #ab150077). .. The nuclei were counterstained with CCK-8 Assay:Article Title: A novel combination therapy with Cabozantinib and Honokiol effectively inhibits c-Met-Nrf2-induced renal tumor growth through increased oxidative stress Article Snippet: Anti-β-actin (Cat. No. #A2228) was obtained from Sigma-Aldrich (Burlington, MA). .. Anti-Rubicon (Cat No. #Ab92388) and the other:Article Title: Low-dose daylight exposure induces nitric oxide release and maintains cell viability in vitro Article Snippet: Assessment of cell survival after light exposure was performed using a Cell Culture:Article Title: Biocompatible Nanostructured Silver-Incorporated Implant Surfaces Show Effective Antibacterial, Osteogenic, and Anti-Inflammatory Effects in vitro and in Rat Model Article Snippet: .. At the designated time points, the media were changed to 500 μL Incubation:Article Title: PCBP2 as an intrinsic aging factor regulates the senescence of hBMSCs through the ROS-FGF2 signaling axis Article Snippet: .. Then, 10 μL of |


