anti eef2 (Danaher Inc)
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Anti Eef2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab91335/Anti-EGFR+(phospho+Y1068)+antibody/pm28386023-223-72-75
Average 99 stars, based on 356 article reviews
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Incubation:Article Title: BMP4 upregulates glycogen synthesis through the SMAD/SLC2A1 (GLUT1) signaling axis in hepatocellular carcinoma (HCC) cells Article Snippet: .. The proteins of interest were assessed by being incubated with the following primary antibodies at 4 °C overnight: anti-GYS1( Article Title: Immuno-PET Detects Antibody-Drug Potency on Coadministration with Statins. Article Snippet: The membranes were incubated in 5% (m/v) milk (Bio-Rad) or bovine serum albumin (Sigma) in tris-buffered saline buffer-polysorbate (EZ BioResearch). .. The membranes were then incubated with the primary antibodies: mouse antib-actin, 1:10,000 (A1978; Sigma); rabbit anti-HER2, 1:800 (ab131490; Abcam); rabbit anti-HER2 phospho Y1139, 1:500 (ab53290; Abcam); rabbit anti–epidermal growth factor receptor (EGFR), 1:1,000 (ab52894; Abcam); rabbit anti-EGFR phospho Y1068, 1:500 ( Article Title: Clinicopathologic Dissociation: Robust Lafora Body Accumulation in Malin KO Mice Without Observable Changes in Home-cage Behavior Article Snippet: For co-immunostaining, paraffin-embedded brain sections were deparaffinized and rehydrated by processing with xylene, decreasing concentrations of ethanol in water, and subjected to antigen retrieval using citrate buffer pH6.0 (C9999, Sigma-Aldrich). .. Sections were blocked with 5% normal donkey serum (in 0.1% Triton X-100, PBS) for 1 h and incubated for 48 h at 4°C with primary antibodies diluted in blocking solution, including those targeted against Article Title: Epigenetic deregulation of MLF1 drives intrahepatic cholangiocarcinoma progression through EGFR/AKT and Wnt/β-catenin signaling Article Snippet: .. After blocking, the membranes were incubated first with anti-MLF1 (1:500, Santa Cruz, sc-514294), anti–DNA methyltransferase (DNMT)1 (1:500, Santa Cruz, sc-271729), anti-DNMT3A (1:1000, Santa Cruz, sc-365769), anti-DNMT3B (1:1000, Santa Cruz, sc-376043), anti-AKT (1:1000, CST, #4691), anti-phospho-AKT(Ser473, 1:1000, CST, #4060), anti-β-catenin (1:1000, CST, #8480), anti-phospho-β-catenin (Ser675, 1:1000, CST, #4176), anti-E-cadherin (1:1000, CST, #3195), anti-N-cadherin (1:1000, CST, #13116), anti-vimentin (1:1000, Abcam, ab92547), anti-LRP6 (1:1000, CST, #3395), anti-phospho-LRP6 (Ser1490, 1:1000, CST, #2568), anti-Bcl2 (1:1000, Abcam, ab32124), anti-Bcl-XL (1:1000, Abcam, ab32370), anti-EGFR (1:3000, Abcam, ab52894), Blocking Assay:Article Title: Clinicopathologic Dissociation: Robust Lafora Body Accumulation in Malin KO Mice Without Observable Changes in Home-cage Behavior Article Snippet: For co-immunostaining, paraffin-embedded brain sections were deparaffinized and rehydrated by processing with xylene, decreasing concentrations of ethanol in water, and subjected to antigen retrieval using citrate buffer pH6.0 (C9999, Sigma-Aldrich). .. Sections were blocked with 5% normal donkey serum (in 0.1% Triton X-100, PBS) for 1 h and incubated for 48 h at 4°C with primary antibodies diluted in blocking solution, including those targeted against Article Title: Epigenetic deregulation of MLF1 drives intrahepatic cholangiocarcinoma progression through EGFR/AKT and Wnt/β-catenin signaling Article Snippet: .. After blocking, the membranes were incubated first with anti-MLF1 (1:500, Santa Cruz, sc-514294), anti–DNA methyltransferase (DNMT)1 (1:500, Santa Cruz, sc-271729), anti-DNMT3A (1:1000, Santa Cruz, sc-365769), anti-DNMT3B (1:1000, Santa Cruz, sc-376043), anti-AKT (1:1000, CST, #4691), anti-phospho-AKT(Ser473, 1:1000, CST, #4060), anti-β-catenin (1:1000, CST, #8480), anti-phospho-β-catenin (Ser675, 1:1000, CST, #4176), anti-E-cadherin (1:1000, CST, #3195), anti-N-cadherin (1:1000, CST, #13116), anti-vimentin (1:1000, Abcam, ab92547), anti-LRP6 (1:1000, CST, #3395), anti-phospho-LRP6 (Ser1490, 1:1000, CST, #2568), anti-Bcl2 (1:1000, Abcam, ab32124), anti-Bcl-XL (1:1000, Abcam, ab32370), anti-EGFR (1:3000, Abcam, ab52894), Binding Assay:Article Title: Clinicopathologic Dissociation: Robust Lafora Body Accumulation in Malin KO Mice Without Observable Changes in Home-cage Behavior Article Snippet: For co-immunostaining, paraffin-embedded brain sections were deparaffinized and rehydrated by processing with xylene, decreasing concentrations of ethanol in water, and subjected to antigen retrieval using citrate buffer pH6.0 (C9999, Sigma-Aldrich). .. Sections were blocked with 5% normal donkey serum (in 0.1% Triton X-100, PBS) for 1 h and incubated for 48 h at 4°C with primary antibodies diluted in blocking solution, including those targeted against Membrane:Article Title: Kras oncogene ablation prevents resistance in advanced lung adenocarcinomas. Article Snippet: Protein extraction was performed in protein lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% NP-40) supplemented with a cocktail of protease and phosphatase inhibitors (cOmplete Mini, Roche; Phosphatase Inhibitor Cocktail 2 and 3, MilliporeSigma). .. A total of 30 μg of protein extracts was separated on NuPAGE 4%–12% BisTris Midi Gels (Invitrogen), transferred to a nitrocellulose blotting membrane (GE Healthcare), and blotted with antibodies against the following: EGFR (Abcam, ab52894), |


