collagen i (Abcam)
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Collagen I, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 246 article reviews
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Electrophoresis:Article Title: Injury induced renal fibrosis promotes cystogenesis and cyst growth in adult mice with autosomal dominant polycystic kidney disease Article Snippet: Protein samples were dissolved in 5x SDS-PAGE loading buffer (P0015L, Beyotime Biotech, Nantong, China), which were further subjected to SDS-PAGE gel electrophoresis. .. After electrophoresis, proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk, 20mM Tris-HCl, 150mMNaCl, PH=8.0, 0.01%Tween 20) for 1 hour at room temperature and was followed by incubation with anti-EZH2 (1:1000, 5246S, Cell Signaling Technology) antibody, anti-fibronectin (1:1000, ab23750, Abcam), anti-pSmad3 (1:1000, ET1609-41, HUABIO), Membrane:Article Title: Injury induced renal fibrosis promotes cystogenesis and cyst growth in adult mice with autosomal dominant polycystic kidney disease Article Snippet: Protein samples were dissolved in 5x SDS-PAGE loading buffer (P0015L, Beyotime Biotech, Nantong, China), which were further subjected to SDS-PAGE gel electrophoresis. .. After electrophoresis, proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk, 20mM Tris-HCl, 150mMNaCl, PH=8.0, 0.01%Tween 20) for 1 hour at room temperature and was followed by incubation with anti-EZH2 (1:1000, 5246S, Cell Signaling Technology) antibody, anti-fibronectin (1:1000, ab23750, Abcam), anti-pSmad3 (1:1000, ET1609-41, HUABIO), Incubation:Article Title: Injury induced renal fibrosis promotes cystogenesis and cyst growth in adult mice with autosomal dominant polycystic kidney disease Article Snippet: Protein samples were dissolved in 5x SDS-PAGE loading buffer (P0015L, Beyotime Biotech, Nantong, China), which were further subjected to SDS-PAGE gel electrophoresis. .. After electrophoresis, proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk, 20mM Tris-HCl, 150mMNaCl, PH=8.0, 0.01%Tween 20) for 1 hour at room temperature and was followed by incubation with anti-EZH2 (1:1000, 5246S, Cell Signaling Technology) antibody, anti-fibronectin (1:1000, ab23750, Abcam), anti-pSmad3 (1:1000, ET1609-41, HUABIO), Article Title: Astragaloside IV restrains pyroptosis and fibrotic development of pulmonary artery smooth muscle cells to ameliorate pulmonary artery hypertension through the PHD2/HIF1α signaling pathway Article Snippet: .. The corresponding blots were cut prior to incubation with antibodies, blocked with 5% skim milk for 1 h at room temperature, and incubated with primary antibodies against NLRP3 (Abcam, #ab263899), cleaved Caspase-1 (Immunoway, Beijing, China, #YC0003), Fibronectin (Abcam, #ab268020), Article Title: Rivaroxaban, a direct inhibitor of coagulation factor Xa, attenuates adverse cardiac remodeling in rats by regulating the PAR-2 and TGF-β1 signaling pathways Article Snippet: .. After blocking with 5% BSA, the membranes were incubated overnight at 4 °C with specific antibodies against the following proteins: PAR-2, 1:1000, Bioss, bs-1178R; TGFβ1, 1:1000, Abcam, ab179695; α-SMA, 1:1000, Cell Signaling Technology, 19245; p-Smad2, 1:1000, Bioss, bs-3419R; Smad2, 1:1000, CST, 5339S; p-Smad3, 1:1000, abclonal, AP0727; Smad3, 1:1000, abclonal, A19115; Blocking Assay:Article Title: Injury induced renal fibrosis promotes cystogenesis and cyst growth in adult mice with autosomal dominant polycystic kidney disease Article Snippet: Protein samples were dissolved in 5x SDS-PAGE loading buffer (P0015L, Beyotime Biotech, Nantong, China), which were further subjected to SDS-PAGE gel electrophoresis. .. After electrophoresis, proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk, 20mM Tris-HCl, 150mMNaCl, PH=8.0, 0.01%Tween 20) for 1 hour at room temperature and was followed by incubation with anti-EZH2 (1:1000, 5246S, Cell Signaling Technology) antibody, anti-fibronectin (1:1000, ab23750, Abcam), anti-pSmad3 (1:1000, ET1609-41, HUABIO), Article Title: Rivaroxaban, a direct inhibitor of coagulation factor Xa, attenuates adverse cardiac remodeling in rats by regulating the PAR-2 and TGF-β1 signaling pathways Article Snippet: .. After blocking with 5% BSA, the membranes were incubated overnight at 4 °C with specific antibodies against the following proteins: PAR-2, 1:1000, Bioss, bs-1178R; TGFβ1, 1:1000, Abcam, ab179695; α-SMA, 1:1000, Cell Signaling Technology, 19245; p-Smad2, 1:1000, Bioss, bs-3419R; Smad2, 1:1000, CST, 5339S; p-Smad3, 1:1000, abclonal, AP0727; Smad3, 1:1000, abclonal, A19115; other:Article Title: Aggravated hepatic fibrosis induced by phenylalanine and tyrosine was ameliorated by chitooligosaccharides supplementation Article Snippet: Type I collagen (Col1) , Abcam Technology , ab260043, AB_2922767. |


