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fviii  (Abcam)


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    Structured Review

    Abcam fviii
    Fviii, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab78852/Anti-Factor+VIII+antibody/pmc08265364-124-11-14
    Average 99 stars, based on 74 article reviews
    fviii - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Immunohistochemical staining:

    Article Title: Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation
    Article Snippet: .. Immunohistochemical staining was performed using paraffin-embedded sections with antibodies against factor VIII-related antigen (1:100, ab236284, Abcam), stromal cell-derived factor-1-alpha (SDF1-α) (1:100, ab25117, Abcam, Cambridge, United Kingdom), Interleukin-1-beta (IL-1-β) (1:100, ab2105, Abcam), tumor necrosis factor-alpha (TNF-α) (1:50, ab1793, Abcam), transforming growth factor-beta (TGF-β) (1:100, ab66043, Abcam), and vascular endothelial growth factor (VEGF) (1:200, ab1316, Abcam). ..

    Staining:

    Article Title: Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation
    Article Snippet: .. Immunohistochemical staining was performed using paraffin-embedded sections with antibodies against factor VIII-related antigen (1:100, ab236284, Abcam), stromal cell-derived factor-1-alpha (SDF1-α) (1:100, ab25117, Abcam, Cambridge, United Kingdom), Interleukin-1-beta (IL-1-β) (1:100, ab2105, Abcam), tumor necrosis factor-alpha (TNF-α) (1:50, ab1793, Abcam), transforming growth factor-beta (TGF-β) (1:100, ab66043, Abcam), and vascular endothelial growth factor (VEGF) (1:200, ab1316, Abcam). ..

    Article Title: Study on promoting the regeneration of grafted fat by cell-assisted lipotransfer
    Article Snippet: .. Anti-Factor VIII (Abcam, USA) was used to identify differentiated hemangioendothelial cells by immunofluorescence staining. ..

    Immunofluorescence:

    Article Title: Study on promoting the regeneration of grafted fat by cell-assisted lipotransfer
    Article Snippet: .. Anti-Factor VIII (Abcam, USA) was used to identify differentiated hemangioendothelial cells by immunofluorescence staining. ..

    Bioprocessing:

    Article Title: Endogenous Bok is stable at the endoplasmic reticulum membrane and does not mediate proteasome inhibitor-induced apoptosis
    Article Snippet: Rabbit antibodies used were: anti-IP 3 R1, anti-IP 3 R2 , anti-erlin2, anti-gp78, anti-Hrd1 , anti-Mcl-1 #D35A5, anti-Bcl-2 #D17C4, anti-caspase-3 #9662, anti-Bak #12105, anti-Bax #2772, anti-LC3A/B #4108, anti-PARP #9542, anti-caspase-8 #8592 (Cell Signaling Technology), anti-Bok A (raised against amino acids 19–32 of mouse Bok) ( ; ), anti-Bok B (Abcam # 186745, raised against amino acids 1–150 of human Bok), and anti-transaldolase ( ). .. Mouse monoclonal antibodies used were: anti-CHOP #7351, anti-ube2J1 #100624 (Santa Cruz Biotechnology Inc.), anti-ubiquitin clone FK2 (BioMol), anti-FLAG epitope clone M2 #F3165 (Sigma), anti-HA epitope clone HA11 (Covance), anti-IP 3 R3 #610313 (BD Transduction Labs), anti-p97 #10R-P104A (Fitzgerald), anti-cytochrome c #13575 and anti-complex V/III #14748/14745 (Abcam). .. Horseradish peroxidase-conjugated secondary antibodies, protease inhibitors, Triton X-100, CHAPS, brefeldin A (BFA), tunicamycin (TN), thapsigargin (TG), bafilomycinA1 (BAFA1), and cycloheximide (CHX) were purchased from Sigma.

    Incubation:

    Article Title: Hsa-miR-5581-3p and Hsa-miR-542-3p Target the F8 Gene in Hemophilia A without F8 Mutations
    Article Snippet: .. Primary antibodies against GAPDH (ab8245, 1:5000, Abcam, Cambridge, MA, USA) and FVIII (ab171825, 1:1000, Abcam) were incubated with the membrane on a shaker for 1 h at room temperature, followed by incubation of secondary antibody for 1 h at room temperature. ..

    Membrane:

    Article Title: Hsa-miR-5581-3p and Hsa-miR-542-3p Target the F8 Gene in Hemophilia A without F8 Mutations
    Article Snippet: .. Primary antibodies against GAPDH (ab8245, 1:5000, Abcam, Cambridge, MA, USA) and FVIII (ab171825, 1:1000, Abcam) were incubated with the membrane on a shaker for 1 h at room temperature, followed by incubation of secondary antibody for 1 h at room temperature. ..



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    Image Search Results


    Identification of HemECs. The expression of CD31, CD34, factor VIII, and GLUT1 in HemECs was studied using immunofluorescence staining. Positive expression was observed for CD31, CD34, factor VIII, and GLUT1 (A, B, C and D) . The cytoplasm and nucleus were stained with FITC (green) and DAPI (blue), respectively. Scale bar = 0.05 mm.

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    Figure Lengend Snippet: Identification of HemECs. The expression of CD31, CD34, factor VIII, and GLUT1 in HemECs was studied using immunofluorescence staining. Positive expression was observed for CD31, CD34, factor VIII, and GLUT1 (A, B, C and D) . The cytoplasm and nucleus were stained with FITC (green) and DAPI (blue), respectively. Scale bar = 0.05 mm.

    Article Snippet: The cells were treated with primary antibodies overnight at 4 °C: CD31 (1:50; Proteintech; 11265-1-AP), CD34 (1:50; Proteintech; 14486-1-AP), factor VIII (Abcam; 1:500; ab275376), and GLUT1 (1:50; Proteintech; 21829-1-AP).

    Techniques: Expressing, Immunofluorescence, Staining

    Representative images of histology. (A) Hematoxylin and eosin(H&E) staining(40X). (B) Immunohistochemical staining of factor VIII(40X). (C) Alcian-blue staining(40X). (D) Picrosirius-red staining(10X). Scale bars depict 100µm.

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    Figure Lengend Snippet: Representative images of histology. (A) Hematoxylin and eosin(H&E) staining(40X). (B) Immunohistochemical staining of factor VIII(40X). (C) Alcian-blue staining(40X). (D) Picrosirius-red staining(10X). Scale bars depict 100µm.

    Article Snippet: Used antibodies included biglycan (Biglycan) antibody (K008381P, Solarbio, Beijing), factor VIII antibody (AB275376, Abcam, UK), MMP-3 (K111452P, Solarbio, Beijing), and IL-6 (KOO9639P, Solarbio, Beijing).

    Techniques: Staining, Immunohistochemical staining