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c myc  (Danaher Inc)


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    Structured Review

    Danaher Inc c myc
    C Myc, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab78852/Recombinant+Anti-EGFR+antibody/pm32061924-83-76-97
    Average 99 stars, based on 1379 article reviews
    c myc - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: Signature-driven repurposing of Midostaurin for combination with MEK1/2 and KRASG12C inhibitors in lung cancer
    Article Snippet: The following rabbit primary antibodies were used: MYC (Abcam, ab32072) and CD8 (Cell Signalling, 98941).

    Staining:

    Article Title: MYC is a clinically significant driver of mTOR inhibitor resistance in breast cancer
    Article Snippet: BlueEye Prestained Protein Marker (#PS-104; Jena Bioscience) and 20 μg of protein were separated on NuPAGE 4-12% Bis-Tris Mini Protein Gels (#NP0323; Thermo Fisher Scientific) using NuPAGE MOPS SDS Running Buffer (#NP0001; Thermo Fisher Scientific), and transferred overnight at 4°C onto Nitrocellulose Membranes (#88018; Thermo Fisher Scientific) in transfer buffer (25 mM Tris, 2 M Glycine, 20% methanol in demineralized water). .. Membranes were stained with Ponceau S (#ab270042; Abcam) and imaged with Fusion FX (Vilber), blocked in 5% bovine serum albumin (BSA; #A8022; Sigma-Aldrich) in PBS-T (0.05% Tween-20), and incubated in 5% BSA in PBS-T overnight at 4°C with the following primary antibodies: β-actin (1:10,000, #A5441; Sigma-Aldrich), C-MYC (1:1,000, #ab32072, Abcam), and phospho (T37/T46)-4E-BP1 (#2855), 4E-BP1 (#9644), phospho (S406)-eIF4B (#8151), phospho (S422)-eIF4B (#3591), eIF4B (#3592), phospho (S235/S236)-S6 ribosomal protein (#2211), and S6 ribosomal protein (all 1:1,000, #2217; all Cell Signaling Technology). .. Membranes were washed with PBS-T and incubated with anti-rabbit HRP (1:10,000, #P0448; DAKO) or anti-mouse HRP (1:10,000, #G-21040; Thermo Fisher Scientific) secondary antibodies in 5% BSA in PBS-T for 1 h at room temperature.

    Article Title: MYC is a clinically significant driver of mTOR inhibitor resistance in breast cancer
    Article Snippet: .. Tissues were formalin-fixed and paraffin-embedded (FFPE) by routine procedures and sectioned for H&E histochemical staining and IHC stainings, which were performed as previously described ( ) using primary antibodies against E-cadherin (1:200, #3195; Cell Signaling Technology), C-MYC (1:100, #ab32072; Abcam), MHC-II (1:100, #NBP2-21789; Novusbio), CD4 (1:1,000, #14-9766-82; eBiosience), FOXP3 (1:200, #14-5773; eBioscience), phospho STAT1 (1:800, #9167; Cell Signaling Technology), CD3 (1:600, #RM-9107-S1; Thermo Fisher Scientific), LY6-G (1:150, #551459; BD Pharmingen), B-220 (1:4,000, #557390; BD Pharmigen), CD8 (1:2,000, #14-0808-82; eBioscience), or NKp46 (1:400, #AF2225; R&D Systems). .. For E-cadherin, C-MYC, MHC-II, CD4, phospho STAT1, CD3, CD8, and NKp46 IHC stainings, antigen retrieval was done with Tris-EDTA at pH 9; for FOXP3 and B-220 IHC stainings, it was done with Citrate Buffer (#CBB999; Scytek); and for LY6-G, it was done with 20 μg/ml Proteinase-K (#P6556; Sigma-Aldrich).

    Incubation:

    Article Title: MYC is a clinically significant driver of mTOR inhibitor resistance in breast cancer
    Article Snippet: BlueEye Prestained Protein Marker (#PS-104; Jena Bioscience) and 20 μg of protein were separated on NuPAGE 4-12% Bis-Tris Mini Protein Gels (#NP0323; Thermo Fisher Scientific) using NuPAGE MOPS SDS Running Buffer (#NP0001; Thermo Fisher Scientific), and transferred overnight at 4°C onto Nitrocellulose Membranes (#88018; Thermo Fisher Scientific) in transfer buffer (25 mM Tris, 2 M Glycine, 20% methanol in demineralized water). .. Membranes were stained with Ponceau S (#ab270042; Abcam) and imaged with Fusion FX (Vilber), blocked in 5% bovine serum albumin (BSA; #A8022; Sigma-Aldrich) in PBS-T (0.05% Tween-20), and incubated in 5% BSA in PBS-T overnight at 4°C with the following primary antibodies: β-actin (1:10,000, #A5441; Sigma-Aldrich), C-MYC (1:1,000, #ab32072, Abcam), and phospho (T37/T46)-4E-BP1 (#2855), 4E-BP1 (#9644), phospho (S406)-eIF4B (#8151), phospho (S422)-eIF4B (#3591), eIF4B (#3592), phospho (S235/S236)-S6 ribosomal protein (#2211), and S6 ribosomal protein (all 1:1,000, #2217; all Cell Signaling Technology). .. Membranes were washed with PBS-T and incubated with anti-rabbit HRP (1:10,000, #P0448; DAKO) or anti-mouse HRP (1:10,000, #G-21040; Thermo Fisher Scientific) secondary antibodies in 5% BSA in PBS-T for 1 h at room temperature.

    Article Title: USP13 drives lung squamous cell carcinoma by switching lung club cell lineage plasticity
    Article Snippet: .. Membranes were blocked for 1 h in 5% milk followed by overnight incubation with primary antibodies directed against USP13 (Santa Cruz, Cat# sc-514416), NKX2-1 (Abcam, Cat#ab76013), SOX2 (CST, cat#14962), MYC (Abcam, Cat#ab32072), CK5 (CST, Cat#71536), p63 (Abcam, Cat#ab124762), SPC (Abcam, Cat#ab211326), β-actin (Santa Cruz, Cat# sc-47778), FLAG-tag (Sigma-Aldrich, Cat#F1804), Myc-tag (CST, Cat#2276), HA-tag (Thermo Fisher Scientific, Cat#14-6756-63) at 4°C. ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: MYC is a clinically significant driver of mTOR inhibitor resistance in breast cancer
    Article Snippet: .. Tissues were formalin-fixed and paraffin-embedded (FFPE) by routine procedures and sectioned for H&E histochemical staining and IHC stainings, which were performed as previously described ( ) using primary antibodies against E-cadherin (1:200, #3195; Cell Signaling Technology), C-MYC (1:100, #ab32072; Abcam), MHC-II (1:100, #NBP2-21789; Novusbio), CD4 (1:1,000, #14-9766-82; eBiosience), FOXP3 (1:200, #14-5773; eBioscience), phospho STAT1 (1:800, #9167; Cell Signaling Technology), CD3 (1:600, #RM-9107-S1; Thermo Fisher Scientific), LY6-G (1:150, #551459; BD Pharmingen), B-220 (1:4,000, #557390; BD Pharmigen), CD8 (1:2,000, #14-0808-82; eBioscience), or NKp46 (1:400, #AF2225; R&D Systems). .. For E-cadherin, C-MYC, MHC-II, CD4, phospho STAT1, CD3, CD8, and NKp46 IHC stainings, antigen retrieval was done with Tris-EDTA at pH 9; for FOXP3 and B-220 IHC stainings, it was done with Citrate Buffer (#CBB999; Scytek); and for LY6-G, it was done with 20 μg/ml Proteinase-K (#P6556; Sigma-Aldrich).

    Immunohistochemistry:

    Article Title: MYC is a clinically significant driver of mTOR inhibitor resistance in breast cancer
    Article Snippet: .. Tissues were formalin-fixed and paraffin-embedded (FFPE) by routine procedures and sectioned for H&E histochemical staining and IHC stainings, which were performed as previously described ( ) using primary antibodies against E-cadherin (1:200, #3195; Cell Signaling Technology), C-MYC (1:100, #ab32072; Abcam), MHC-II (1:100, #NBP2-21789; Novusbio), CD4 (1:1,000, #14-9766-82; eBiosience), FOXP3 (1:200, #14-5773; eBioscience), phospho STAT1 (1:800, #9167; Cell Signaling Technology), CD3 (1:600, #RM-9107-S1; Thermo Fisher Scientific), LY6-G (1:150, #551459; BD Pharmingen), B-220 (1:4,000, #557390; BD Pharmigen), CD8 (1:2,000, #14-0808-82; eBioscience), or NKp46 (1:400, #AF2225; R&D Systems). .. For E-cadherin, C-MYC, MHC-II, CD4, phospho STAT1, CD3, CD8, and NKp46 IHC stainings, antigen retrieval was done with Tris-EDTA at pH 9; for FOXP3 and B-220 IHC stainings, it was done with Citrate Buffer (#CBB999; Scytek); and for LY6-G, it was done with 20 μg/ml Proteinase-K (#P6556; Sigma-Aldrich).

    Immunopeptidomics:

    Article Title: MYC is a clinically significant driver of mTOR inhibitor resistance in breast cancer
    Article Snippet: .. Tissues were formalin-fixed and paraffin-embedded (FFPE) by routine procedures and sectioned for H&E histochemical staining and IHC stainings, which were performed as previously described ( ) using primary antibodies against E-cadherin (1:200, #3195; Cell Signaling Technology), C-MYC (1:100, #ab32072; Abcam), MHC-II (1:100, #NBP2-21789; Novusbio), CD4 (1:1,000, #14-9766-82; eBiosience), FOXP3 (1:200, #14-5773; eBioscience), phospho STAT1 (1:800, #9167; Cell Signaling Technology), CD3 (1:600, #RM-9107-S1; Thermo Fisher Scientific), LY6-G (1:150, #551459; BD Pharmingen), B-220 (1:4,000, #557390; BD Pharmigen), CD8 (1:2,000, #14-0808-82; eBioscience), or NKp46 (1:400, #AF2225; R&D Systems). .. For E-cadherin, C-MYC, MHC-II, CD4, phospho STAT1, CD3, CD8, and NKp46 IHC stainings, antigen retrieval was done with Tris-EDTA at pH 9; for FOXP3 and B-220 IHC stainings, it was done with Citrate Buffer (#CBB999; Scytek); and for LY6-G, it was done with 20 μg/ml Proteinase-K (#P6556; Sigma-Aldrich).

    FLAG-tag:

    Article Title: USP13 drives lung squamous cell carcinoma by switching lung club cell lineage plasticity
    Article Snippet: .. Membranes were blocked for 1 h in 5% milk followed by overnight incubation with primary antibodies directed against USP13 (Santa Cruz, Cat# sc-514416), NKX2-1 (Abcam, Cat#ab76013), SOX2 (CST, cat#14962), MYC (Abcam, Cat#ab32072), CK5 (CST, Cat#71536), p63 (Abcam, Cat#ab124762), SPC (Abcam, Cat#ab211326), β-actin (Santa Cruz, Cat# sc-47778), FLAG-tag (Sigma-Aldrich, Cat#F1804), Myc-tag (CST, Cat#2276), HA-tag (Thermo Fisher Scientific, Cat#14-6756-63) at 4°C. ..



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    Image Search Results


    Identification of HemECs. The expression of CD31, CD34, factor VIII, and GLUT1 in HemECs was studied using immunofluorescence staining. Positive expression was observed for CD31, CD34, factor VIII, and GLUT1 (A, B, C and D) . The cytoplasm and nucleus were stained with FITC (green) and DAPI (blue), respectively. Scale bar = 0.05 mm.

    Journal: Heliyon

    Article Title: Itraconazole inhibits proliferation, induces apoptosis, and reduces angiogenesis of hemangioma endothelial cells by downregulating the hedgehog signaling pathway

    doi: 10.1016/j.heliyon.2023.e19244

    Figure Lengend Snippet: Identification of HemECs. The expression of CD31, CD34, factor VIII, and GLUT1 in HemECs was studied using immunofluorescence staining. Positive expression was observed for CD31, CD34, factor VIII, and GLUT1 (A, B, C and D) . The cytoplasm and nucleus were stained with FITC (green) and DAPI (blue), respectively. Scale bar = 0.05 mm.

    Article Snippet: The cells were treated with primary antibodies overnight at 4 °C: CD31 (1:50; Proteintech; 11265-1-AP), CD34 (1:50; Proteintech; 14486-1-AP), factor VIII (Abcam; 1:500; ab275376), and GLUT1 (1:50; Proteintech; 21829-1-AP).

    Techniques: Expressing, Immunofluorescence, Staining

    Representative images of histology. (A) Hematoxylin and eosin(H&E) staining(40X). (B) Immunohistochemical staining of factor VIII(40X). (C) Alcian-blue staining(40X). (D) Picrosirius-red staining(10X). Scale bars depict 100µm.

    Journal: Frontiers in Endocrinology

    Article Title: Evaluating the role of type 2 diabetes mellitus in rotator cuff tendinopathy: Development and analysis of a novel rat model

    doi: 10.3389/fendo.2022.1042878

    Figure Lengend Snippet: Representative images of histology. (A) Hematoxylin and eosin(H&E) staining(40X). (B) Immunohistochemical staining of factor VIII(40X). (C) Alcian-blue staining(40X). (D) Picrosirius-red staining(10X). Scale bars depict 100µm.

    Article Snippet: Used antibodies included biglycan (Biglycan) antibody (K008381P, Solarbio, Beijing), factor VIII antibody (AB275376, Abcam, UK), MMP-3 (K111452P, Solarbio, Beijing), and IL-6 (KOO9639P, Solarbio, Beijing).

    Techniques: Staining, Immunohistochemical staining