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anti tnf α  (Danaher Inc)


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    Danaher Inc anti tnf α
    Anti Tnf α, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 2120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab66579/Anti-TNF+alpha+antibody/pmc06274242-127-5-8
    Average 99 stars, based on 2120 article reviews
    anti tnf α - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Drug Repositioning of Metformin Encapsulated in PLGA Combined with Photothermal Therapy Ameliorates Rheumatoid Arthritis
    Article Snippet: .. Sectioned samples were deparaffinized and incubated with the following primary antibodies: CD3e (Invitrogen, USA; MA5-16762), IL-22R (Abcam, Cambridge, UK; ab203211), TNF-α (Abcam, Cambridge, UK; ab1793), F4/80 (Abcam, Cambridge, UK; ab6640), CD86 (Invitrogen, USA; 13–0862-82), and Dectin-1 (Abcam, Cambridge, UK; ab140039). .. Subsequently, the samples were treated with the following secondary antibodies: anti-mouse IgG Alexa Fluor 594 (Abcam, Cambridge, UK; ab150116) for TNF-α; anti-rabbit IgG Alexa Fluor 488 (Abcam, Cambridge, UK; ab150077) for CD86 and Dectin-1; anti-rat IgG Alexa Fluor 594 (Abcam, Cambridge, UK; ab150160) for F4/80; and anti-rat IgG Alexa Fluor 488 (Invitrogen, USA; PA1-84761) and anti-rabbit IgG Alexa Fluor 594 (Invitrogen, USA; A-11012) for IL-22R.

    Article Title: Mechanisms of resveratrol against diabetic wound by network pharmacology and experimental validation
    Article Snippet: .. Afterwards, using TBST buffer including 5% bovine serum albumin to block the membranes and then incubated with the primary antibodies IL-1β (ab254360, Abcam), IL-6 (ab290735, Abcam), IL-18 (10663-1-AP, Proteintech), TNF-α (ab205587, Abcam), RAGE (WL01514, Wanleibio), NF-κB p65 (A19653, ABclonal), p-NF-κB p65 (3033, CST), β-actin (20536-1-AP, Proteintech) overnight at 4 °C. .. Then, the secondary antibody (ZB-2301) was incubated with the membranes for 1 h. Finally, the bands were detected using the ECL kit (E412-01, Vazmye), and the image was analysed using ImageJ software.

    Immunostaining:

    Article Title: Enhanced Intervertebral Disc Repair via Genetically Engineered Mesenchymal Stem Cells with Tetracycline Regulatory System
    Article Snippet: .. For immunostaining, the sections were dewaxed, rehydrated, and stained with primary antibodies against aggrecan (1:1000) (ab36861; Abcam, Cambridge, UK), type II collagen (1:100) (ab34712; Abcam), CGRP (1:200) (ab47027; Abcam), Tie2 (1:200) (NBP2-20636; Novus Biologicals, Littleton, CO, USA), Brachyury (1:200) (sc-166962; Santa Cruz Biotechnology), human nuclei antibody (1:200) (MAB1281; Sigma-Aldrich), TNF-α (1:200) (ab6671; Abcam), MMP-13 (1:200) (ab39012; Abcam), and IL-1β (1:200) (AF-501-NA; Novus Biologicals). .. After 24 h of incubation, the sections were rinsed with 1×PBS containing 0.1% Tween 20 and subsequently incubated with the secondary antibody anti-Rb horseradish peroxidase (Roche Diagnostics Ltd., Basel, Switzerland), as well as Alexa Fluor 488- (1:400) (A11034; Invitrogen), Alexa Fluor 488- (1:400) (A11029; Invitrogen), Alexa Fluor 568- (1:400) (A10042; Invitrogen), and Alexa Fluor 647 (1:400) (A21469; Invitrogen)-conjugated secondary antibodies.

    Staining:

    Article Title: Enhanced Intervertebral Disc Repair via Genetically Engineered Mesenchymal Stem Cells with Tetracycline Regulatory System
    Article Snippet: .. For immunostaining, the sections were dewaxed, rehydrated, and stained with primary antibodies against aggrecan (1:1000) (ab36861; Abcam, Cambridge, UK), type II collagen (1:100) (ab34712; Abcam), CGRP (1:200) (ab47027; Abcam), Tie2 (1:200) (NBP2-20636; Novus Biologicals, Littleton, CO, USA), Brachyury (1:200) (sc-166962; Santa Cruz Biotechnology), human nuclei antibody (1:200) (MAB1281; Sigma-Aldrich), TNF-α (1:200) (ab6671; Abcam), MMP-13 (1:200) (ab39012; Abcam), and IL-1β (1:200) (AF-501-NA; Novus Biologicals). .. After 24 h of incubation, the sections were rinsed with 1×PBS containing 0.1% Tween 20 and subsequently incubated with the secondary antibody anti-Rb horseradish peroxidase (Roche Diagnostics Ltd., Basel, Switzerland), as well as Alexa Fluor 488- (1:400) (A11034; Invitrogen), Alexa Fluor 488- (1:400) (A11029; Invitrogen), Alexa Fluor 568- (1:400) (A10042; Invitrogen), and Alexa Fluor 647 (1:400) (A21469; Invitrogen)-conjugated secondary antibodies.

    Article Title: Methods for treating heart disease via redirected T cell immunotherapies
    Article Snippet: Angiotensin II (A9525-50MG), phenylephrine hydrochloride (P6126-10G), tamoxifen (T5648-1G), and corn oil (C8267) were obtained from Millipore Sigma. .. Picro Sirius Red Stain Kit (ab150681), Anti-Fibroblast activation protein, alpha antibody (ab207178), and GFP (ab6673) were obtained from Abcam. .. Sterile saline (0.9% sodium chloride, 00409488850) was obtained from Hospira (Lake Forest, Ill.).

    Blocking Assay:

    Article Title: Mechanisms of resveratrol against diabetic wound by network pharmacology and experimental validation
    Article Snippet: .. Afterwards, using TBST buffer including 5% bovine serum albumin to block the membranes and then incubated with the primary antibodies IL-1β (ab254360, Abcam), IL-6 (ab290735, Abcam), IL-18 (10663-1-AP, Proteintech), TNF-α (ab205587, Abcam), RAGE (WL01514, Wanleibio), NF-κB p65 (A19653, ABclonal), p-NF-κB p65 (3033, CST), β-actin (20536-1-AP, Proteintech) overnight at 4 °C. .. Then, the secondary antibody (ZB-2301) was incubated with the membranes for 1 h. Finally, the bands were detected using the ECL kit (E412-01, Vazmye), and the image was analysed using ImageJ software.

    Article Title: PSMC5 regulates microglial polarization and activation in LPS-induced cognitive deficits and motor impairments by interacting with TLR4
    Article Snippet: Immunofluorescence analyses were carried out on 10 μm-thick brain slice sequential sections prepared on a microtome (Leica CM 1850; Leica Microsystems, Seoul, Korea). .. The brain sections were transferred to 30% sucrose solution and permeabilized with Triton X-100 (0.3% in TBST) at room temperature for 10 min followed by three washes in 0.025% TBST, and blocked with 1% bovine serum albumin (BSA) in TBST for 1 h. After blocking, the sections were incubated at 4 °C overnight with primary antibodies against TNF-α (Abcam, Inc., Cambridge, MA, USA), chitinase 3 like protein 3 (YM-1; Abcam, Inc., Cambridge, MA, USA), or ionized calcium-binding adapter protein 1 (IBA1; Millipore Corp., Billerica, MA, USA). .. After washing three times with TBST, the sections were incubated with the appropriate TRIC-conjugated and DyLight 488- or 546-conjugated secondary antibodies (Invitrogen-Molecular Probes, Carlsbad, CA) at room temperature in the dark for 60 min. Nuclear staining was performed with a 4′, 6-diamidino-2- phenylindole (DAPI) staining solution for 10 min at room temperature.

    Activation Assay:

    Article Title: Methods for treating heart disease via redirected T cell immunotherapies
    Article Snippet: Angiotensin II (A9525-50MG), phenylephrine hydrochloride (P6126-10G), tamoxifen (T5648-1G), and corn oil (C8267) were obtained from Millipore Sigma. .. Picro Sirius Red Stain Kit (ab150681), Anti-Fibroblast activation protein, alpha antibody (ab207178), and GFP (ab6673) were obtained from Abcam. .. Sterile saline (0.9% sodium chloride, 00409488850) was obtained from Hospira (Lake Forest, Ill.).



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