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Structured Review

Abcam iba 1
Iba 1, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab66579/Anti-Lysozyme+antibody/pm29091306-111-6-26
Average 99 stars, based on 719 article reviews
iba 1 - by Bioz Stars, 2026-09
99/100 stars

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Incubation:

Article Title: Differential Effects of Regulatory T Cells in the Meninges and Spinal Cord of Male and Female Mice with Neuropathic Pain
Article Snippet: Slides were then incubated in PBS with 0.3% Triton-X (Sigma-Aldrich, Saint Louis, MO, USA) for 30 min at RT, then replaced by a blocking solution containing PBS with 10% normal donkey serum (NDS) and 0.3% Triton X-100 (both from Sigma-Aldrich) for another 30 min at RT. .. Sections were incubated with the following primary antibodies diluted in PBS containing 2% NDS and 0.1% Triton X-100 overnight at 4 °C; goat anti-mouse IBA-1 (WAKO 011-27991 1:200), rabbit anti-mouse lysozyme (LYZ, Abcam ab108508 1:200), goat anti-mouse CD206 (RnDSystems AF2535 1:400), rabbit anti-P2RY12 (AnaSpec AS-55043A 1:400), rabbit anti-GFAP (Abcam ab7260 1:400), goat anti-C3 (Abcam ab11887 1:100), hamster anti-mouse CD11c (ThermoFisher 14-0114-82 1:100) and rabbit anti-mouse insulin growth-like factor 1 (IGF1, Abcam ab9572 1:100). .. Sections were then washed three times with PBS before adding the following suitable secondary antibodies: Alexa Fluor 488 donkey anti-rabbit/goat/hamster and Alexa Fluor 647 donkey anti-rabbit/goat (1:500; Life Technologies) in PBS with 2% NDS.

Article Title: Sequential inspiratory muscle exercise-noninvasive positive pressure ventilation alleviates oxidative stress in COPD by mediating SOCS5/JAK2/STAT3 pathway
Article Snippet: The proteins were isolated with RIPA and the cut gels were transferred to the PVDF membrane (Millipore, MA, USA). .. Then, membranes were incubated overnight with antibodies against SOCS5 (PA5-21600, Thermo Fisher Scientific), JAK2 (ab108596, Abcam), p-JAK2 (ab32101, Abcam), STAT3 (ab119352, Abcam), p-STAT3 (ab32143, Abcam), and GAPDH (ab8245, Abcam). .. After washed with TBST, membranes were then incubated with secondary antibody (ab7090, Abcam) for 60 min.

Immunofluorescence:

Article Title: Intestinal epithelial pH-sensing receptor GPR65 maintains mucosal homeostasis via regulating antimicrobial defense and restrains gut inflammation in inflammatory bowel disease
Article Snippet: .. After deparaffinization, rehydration, and antigen retrieval, the slides were treated with 0.3% Triton X-100 for 10 min at room temperature and blocked with 10% normal donkey serum for 1 h. Subsequently, immunofluorescence staining was performed with rabbit anti-mouse REG3γ (ab198216, Abcam) or rabbit anti-mouse lysozyme (ab108508, Abcam) overnight at 4°C. ..

Staining:

Article Title: Intestinal epithelial pH-sensing receptor GPR65 maintains mucosal homeostasis via regulating antimicrobial defense and restrains gut inflammation in inflammatory bowel disease
Article Snippet: .. After deparaffinization, rehydration, and antigen retrieval, the slides were treated with 0.3% Triton X-100 for 10 min at room temperature and blocked with 10% normal donkey serum for 1 h. Subsequently, immunofluorescence staining was performed with rabbit anti-mouse REG3γ (ab198216, Abcam) or rabbit anti-mouse lysozyme (ab108508, Abcam) overnight at 4°C. ..

Immunohistochemistry:

Article Title: Partial in vivo reprogramming enables injury-free intestinal regeneration via autonomous Ptgs1 induction
Article Snippet: Fluorescence microscopy (Olympus) was used for imaging samples. .. Following primary antibodies were used for IHC and IF: mouse anti-2A peptide (Millipore, MABS2005, 1:200), rabbit anti-Active YAP1 (Abcam, ab205270, 1:1000), rabbit anti-Avil (Abcam, ab72210, 1:500), rabbit anti-BrdU (Novus Biologicals, NBP2-14890, 1:200), CD44 (BioLegend, 103015, 1:500), rabbit anti-Ki67 (Abcam, ab16667, 1:500), rabbit anti-Lysozyme (Abcam, ab108508, 1:200), mouse anti-Oct4 (BD Biosiences, 611203, 1:500), rabbit anti-Olfm4 (Cell Signaling Technology, 39141, 1:400), rabbit anti-phosphohistone H3 (Cell Signaling Technology, 53348, 1:1000), rat anti-Sca1 (BioLegend, 122501, 1:500), rabbit anti-Sox2 (Millipore, AB5603, 1:400), goat anti-Trop2 (R&D Systems, AF1122, 1:100), mouse anti-Cox1 (Santa Cruz Biotechnology, sc-19998, 1:100), mouse anti-Cox2 (Santa Cruz Biotechnology, sc-19999, 1:100), rabbit anti–E-cadherin (Cell Signaling Technology, 3195, 1:1000), and rabbit anti-Dclk1(Abcam, ab31704, 1:1000). .. For histological analysis, sections were stained with hematoxylin and eosin, dehydrated, and then covered with slide glass using Canada balsam (Sigma-Aldrich).

Western Blot:

Article Title: Kindlin-2 in myoepithelium controls luminal progenitor commitment to alveoli in mouse mammary gland
Article Snippet: .. Antibodies used for western blot analysis in this study were: rabbit anti-Kindlin-2 (Sigma, K3269, 1:1000 dilution); mouse anti-GAPDH (Zhong Shan Jin Qiao, TA-08, 1:1000 dilution); mouse anti-tubulin (Sigma, clone B-5-1-2, 1:1000 dilution); rabbit anti-DDDDK tag (Abcam, ab205606, 1:1000 dilution); rabbit anti-STAT3 (Abcam, ab68153, 1:1000 dilution); rabbit anti-pSTAT3-S727 (Abcam, ab32143, 1:1000 dilution); rabbit anti-pSTAT3-Y705 (Abcam, ab76315, 1:1000 dilution); rabbit anti-Dll1 (Abcam, ab10554, 1:1000 dilution); rabbit anti-JAK2 (Abcam, ab108596, 1:1000 dilution). .. After washing three times in TBST buffer, the membranes were incubated with appropriate HRP-conjugated secondary antibodies at room temperature for 1 h. The Super Signal Chemiluminescence kit (Thermo Fisher Scientific) was used to detect immobilized antibodies.



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