human ifn gamma elispot kit (Danaher Inc)
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human ifn gamma elispot kit
![FIGURE 6. IL-4 and Combo-activated CD40B cells are efficient APC that promote T cell activation and proliferation. (A) Schematic illustration of the general protocol for recognition assays between CD40B cells or CD40L-matured MoDC and Ag-specific CD81 T cells as detailed in the Material and Methods. (B and C) Represen- tative dot plots of the expression of (B) 4-1BB or (C) <t>IFN-g</t> by CD81T cells cocultured with IL- 4, IL-21, Combo-CD40B cells, or MoDC pulsed with 0.001 mM of g209-2M peptide are shown in the left panel. Cells were gated on live CD31CD4CD81T cells. A dose-response curve for all peptide concentrations evaluated is shown in the right panel. T cells only or T cells stimu- lated with PMA/ionomycin were used as controls. Results are represented as mean (SD) (n = 4). (D) IFN-g secretion was quantified in coculture supernatants by ELISA to evaluate MHC-I Ag pre- sentation by IL-4, IL-21, Combo-CD40B cells, or MoDC pulsed with indicated peptide concentra- tions. T cells only or T cells stimulated with PMA/ ionomycin were used as controls. IFN-g secretion was quantified in two technical replicates for each sample and condition. Results are represented as mean (SD) (n = 8). (E) Representative <t>ELISPOT</t> well images containing 5 104 T cells cocultured with autologous CEF-loaded IL-4, IL-21, Combo- CD40B cells, or MoDC. The number of IFN-g1 T cells (SFU) was quantified in two technical replicates for each sample and condition and is presented in the left panel. T cells only or T cells stimulated with PMA/ionomycin were used as controls. SFU (mean [SD]) are pooled from repeated experi- ments (n = 2) from one CEF-reactive donor and are representative of two CEF-reactive donors (Supplemental Fig. 3C). The mean spot size rep- resenting IFN-g secretion was quantified in two technical replicates for each sample and condi- tion. Results are represented as mean (SD) (n = 2 CEF-reactive donor). (F) Representative histo- grams of T cell proliferation in an MLR for the APC/T cell ratio of 1:4. T cell proliferation was assessed by evaluating CTV dilution by flow cytometry after 6 d of culture with indicated stim- ulator cells or controls. Unstimulated T cells or T cells stimulated with anti-CD3, anti-CD28, and IL-2 were used as controls. Division indexes were calculated as mentioned in the Material and Meth- ods. Results are represented as mean (SD) (n = 9). T cell proliferation was assessed in two technical replicates for each sample and condition. Statistical significance of normally distributed data (Shapir- oWilk test) compared with Combo-CD40B cells was calculated using one-way (E) or two-way (BD, F) ANOVA with post hoc Tukey HSD. *p < 0.05, **p < 0.01.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_5061/pm34135061/pm34135061__page11_image1.jpg)
Human Ifn Gamma Elispot Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab62899/Human+Interferon+Gamma+ELISPOT+Kit+1x96+tests/pm34135061-106-6-12
Average 99 stars, based on 8 article reviews
Human Ifn Gamma Elispot Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab62899/Human+Interferon+Gamma+ELISPOT+Kit+1x96+tests/pm34135061-106-6-12
Average 99 stars, based on 8 article reviews
human ifn gamma elispot kit - by Bioz Stars,
2026-09
99/100 stars
Images
1) Product Images from "CD40L-Stimulated B Lymphocytes Are Polarized toward APC Functions after Exposure to IL-4 and IL-21."
Article Title: CD40L-Stimulated B Lymphocytes Are Polarized toward APC Functions after Exposure to IL-4 and IL-21.
Journal: Journal of immunology (Baltimore, Md. : 1950)
doi: 10.4049/jimmunol.2001173
Figure Legend Snippet: FIGURE 6. IL-4 and Combo-activated CD40B cells are efficient APC that promote T cell activation and proliferation. (A) Schematic illustration of the general protocol for recognition assays between CD40B cells or CD40L-matured MoDC and Ag-specific CD81 T cells as detailed in the Material and Methods. (B and C) Represen- tative dot plots of the expression of (B) 4-1BB or (C) IFN-g by CD81T cells cocultured with IL- 4, IL-21, Combo-CD40B cells, or MoDC pulsed with 0.001 mM of g209-2M peptide are shown in the left panel. Cells were gated on live CD31CD4CD81T cells. A dose-response curve for all peptide concentrations evaluated is shown in the right panel. T cells only or T cells stimu- lated with PMA/ionomycin were used as controls. Results are represented as mean (SD) (n = 4). (D) IFN-g secretion was quantified in coculture supernatants by ELISA to evaluate MHC-I Ag pre- sentation by IL-4, IL-21, Combo-CD40B cells, or MoDC pulsed with indicated peptide concentra- tions. T cells only or T cells stimulated with PMA/ ionomycin were used as controls. IFN-g secretion was quantified in two technical replicates for each sample and condition. Results are represented as mean (SD) (n = 8). (E) Representative ELISPOT well images containing 5 104 T cells cocultured with autologous CEF-loaded IL-4, IL-21, Combo- CD40B cells, or MoDC. The number of IFN-g1 T cells (SFU) was quantified in two technical replicates for each sample and condition and is presented in the left panel. T cells only or T cells stimulated with PMA/ionomycin were used as controls. SFU (mean [SD]) are pooled from repeated experi- ments (n = 2) from one CEF-reactive donor and are representative of two CEF-reactive donors (Supplemental Fig. 3C). The mean spot size rep- resenting IFN-g secretion was quantified in two technical replicates for each sample and condi- tion. Results are represented as mean (SD) (n = 2 CEF-reactive donor). (F) Representative histo- grams of T cell proliferation in an MLR for the APC/T cell ratio of 1:4. T cell proliferation was assessed by evaluating CTV dilution by flow cytometry after 6 d of culture with indicated stim- ulator cells or controls. Unstimulated T cells or T cells stimulated with anti-CD3, anti-CD28, and IL-2 were used as controls. Division indexes were calculated as mentioned in the Material and Meth- ods. Results are represented as mean (SD) (n = 9). T cell proliferation was assessed in two technical replicates for each sample and condition. Statistical significance of normally distributed data (Shapir- oWilk test) compared with Combo-CD40B cells was calculated using one-way (E) or two-way (BD, F) ANOVA with post hoc Tukey HSD. *p < 0.05, **p < 0.01.
Techniques Used: Activation Assay, Expressing, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, Flow Cytometry
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