human interferon gamma elispot kit (Danaher Inc)
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human interferon gamma elispot kit
Human Interferon Gamma Elispot Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab62899/Human+Interferon+Gamma+ELISPOT+Kit+1x96+tests/med_rxiv__2022__09__05__22279572-180-12-17
Average 99 stars, based on 8 article reviews
Human Interferon Gamma Elispot Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab62899/Human+Interferon+Gamma+ELISPOT+Kit+1x96+tests/med_rxiv__2022__09__05__22279572-180-12-17
Average 99 stars, based on 8 article reviews
human interferon gamma elispot kit - by Bioz Stars,
2026-09
99/100 stars
Images
1) Product Images from "Distinct immune signatures discriminate SARS-CoV-2 vaccine combinations"
Article Title: Distinct immune signatures discriminate SARS-CoV-2 vaccine combinations
Journal: medRxiv
doi: 10.1101/2022.09.05.22279572
Figure Legend Snippet: (a)Schematic representation of the study protocol. Participants were vaccinated with one of 16 COVID-19 vaccine combination regimens and donated blood at timepoints T1 (4-12 weeks after Dose 1), T2 (two weeks after Dose 2), and T3 (four weeks after Dose 2). PBMCs collected at T1 and T3 were used for IFNγ ELISpot assays and high-dimensional spectral flow cytometry analysis. Sera and plasma collected at T1, T2, and T3 were analysed for anti-S-RBD IgG levels and NAb titres, respectively. Participants were monitored for adverse events. The data collected were analysed using dimensionality reduction, FlowSOM-based clustering algorithms, and statistical testing. (b) Cohort characteristics. Mean number of days and standard deviation after Dose 1 or Dose 2 were calculated from a subset of the total participants, as indicated below each mean.
Techniques Used: Enzyme-linked Immunospot, Flow Cytometry, Clinical Proteomics, Standard Deviation
Figure Legend Snippet: (a,c) Normalized IFNγ production after stimulation of PBMCs with SARS-CoV-2 spike peptide pool, measured by ELISpot assay. (a) Longitudinal IFNγ response at T1 and T3. Black lines show the median. (b) Representative images of one IFNγ ELISpot assay (n=583). (c) Normalized IFNγ responses at T3 after stimulation with SARS-CoV-2 spike peptide pool. Large black dots show the median of each group, and the vertical line spans the interquartile range. The horizontal line indicates the positive cut-off threshold. P-values indicate differences between the respective group and the overall mean of all participants. (a,c) P-values were calculated using the Mann-Whitney-Wilcoxon test and the Benjamini-Hochberg method to control for multiple hypothesis testing. Only statistically significant p-values (p < 0.05) are shown. (d) UMAP showing the FlowSOM-guided manual metaclustering of T cells (CD3+) for all vaccine groups combined. (e) Frequencies of canonical T cell subsets relative to the total number of T cells for each vaccine regimen. (f,g) UMAP of the T cell compartment showing the clusters with frequencies (f) positively or (g) negatively correlated with SARS-CoV-2 spike peptide-induced IFNγ response. (h) Differential marker expression by the specific T cell subclusters compared to the canonical T cell subsets that positively and negatively correlated with SARS-CoV-2 spike peptide-induced IFNγ responses, filtered for markers with at least 0.2 differential expression. The colour shows the Spearman’s rank correlation coefficient (rs), and the bubble size shows the p-value. Only significantly correlated clusters (p < 0.00001) are displayed.
Techniques Used: Enzyme-linked Immunospot, MANN-WHITNEY, Control, Marker, Expressing, Quantitative Proteomics
Related Articles
Enzyme-linked Immunospot:Article Title: CD40L-Stimulated B Lymphocytes Are Polarized toward APC Functions after Exposure to IL-4 and IL-21. Article Snippet: .. ELISPOT was performed as per the Article Title: Efficacy of tiotropium bromide combined with different doses of fluticasone plus salmeterol DPI in the treatment of stable COPD Article Snippet: .. The lung function was assessed by CAT table of COPD assessment test, including forced expiratory volume in 1 s (FEV 1 ), FEV 1 as a percentage of the expected value (FEV 1 %), and FEV 1 /forced vital capacity (FEV 1 /FVC). (4) In each group, the expression levels of serum IL-8 (Human IL-8 ELISA Kit, ab214030, Abcam (Shanghai) Trading Co., Ltd), IL-10 ( Article Title: CD40L-Stimulated B Lymphocytes Are Polarized toward APC Functions after Exposure to IL-4 and IL-21. Article Snippet: .. Cells were discarded and ELISPOT plates were processed as per the Article Title: Characterization of B cell-mediated PD-1/PD-L1 interaction in pancreatic cancer patients. Article Snippet: This article is protected by copyright.. All rights reserved Pancreatic ductal adenocarcinoma (PDAC) is a common type of pancreatic cancer with one of the worst survival rate of all malignancies.. Recent studies have identified that immunosuppressive B cells could employ the PD-1/PD-L1 pathway to suppress antitumor T cell responses, hence, we examined the expression and function of PD-L1 in B cells. Article Title: Distinct immune signatures discriminate SARS-CoV-2 vaccine combinations Article Snippet: .. IFNγ ELISpot analysis was performed using microplates pre-coated with monoclonal IFNγ-specific antibodies ( Article Title: High-dimensional analysis of 16 SARS-CoV-2 vaccine combinations reveals lymphocyte signatures correlating with immunogenicity. Article Snippet: .. IFNγ ELISpot analysis was performed using microplates precoated with monoclonal IFNγ‐specific antibodies ( Expressing:Article Title: Efficacy of tiotropium bromide combined with different doses of fluticasone plus salmeterol DPI in the treatment of stable COPD Article Snippet: .. The lung function was assessed by CAT table of COPD assessment test, including forced expiratory volume in 1 s (FEV 1 ), FEV 1 as a percentage of the expected value (FEV 1 %), and FEV 1 /forced vital capacity (FEV 1 /FVC). (4) In each group, the expression levels of serum IL-8 (Human IL-8 ELISA Kit, ab214030, Abcam (Shanghai) Trading Co., Ltd), IL-10 ( Enzyme-linked Immunosorbent Assay:Article Title: Efficacy of tiotropium bromide combined with different doses of fluticasone plus salmeterol DPI in the treatment of stable COPD Article Snippet: .. The lung function was assessed by CAT table of COPD assessment test, including forced expiratory volume in 1 s (FEV 1 ), FEV 1 as a percentage of the expected value (FEV 1 %), and FEV 1 /forced vital capacity (FEV 1 /FVC). (4) In each group, the expression levels of serum IL-8 (Human IL-8 ELISA Kit, ab214030, Abcam (Shanghai) Trading Co., Ltd), IL-10 ( Isolation:Article Title: Characterization of B cell-mediated PD-1/PD-L1 interaction in pancreatic cancer patients. Article Snippet: This article is protected by copyright.. All rights reserved Pancreatic ductal adenocarcinoma (PDAC) is a common type of pancreatic cancer with one of the worst survival rate of all malignancies.. Recent studies have identified that immunosuppressive B cells could employ the PD-1/PD-L1 pathway to suppress antitumor T cell responses, hence, we examined the expression and function of PD-L1 in B cells. Incubation:Article Title: lncRNA TINCR attenuates the proliferation and invasion, and enhances the apoptosis of cutaneous malignant melanoma cells by regulating the miR-424-5p/LATS1 axis Article Snippet: The membranes were then incubated with the following primary antibodies at 4°C overnight: Anti-LATS1 (1:1,000; cat. no. ab243656; Abcam), anti-phosphorylated (p)-Yes 1 associated transcriptional regulator (YAP; 1:1,000; cat. no. ab76252; Abcam), anti-YAP (1:1,000; cat. no. ab52771; Abcam) and anti-β-actin (1:1,000; cat. no. ab8226; Abcam). .. Following the primary antibody incubation, the membranes were incubated with |
![FIGURE 6. IL-4 and Combo-activated CD40B cells are efficient APC that promote T cell activation and proliferation. (A) Schematic illustration of the general protocol for recognition assays between CD40B cells or CD40L-matured MoDC and Ag-specific CD81 T cells as detailed in the Material and Methods. (B and C) Represen- tative dot plots of the expression of (B) 4-1BB or (C) <t>IFN-g</t> by CD81T cells cocultured with IL- 4, IL-21, Combo-CD40B cells, or MoDC pulsed with 0.001 mM of g209-2M peptide are shown in the left panel. Cells were gated on live CD31CD4CD81T cells. A dose-response curve for all peptide concentrations evaluated is shown in the right panel. T cells only or T cells stimu- lated with PMA/ionomycin were used as controls. Results are represented as mean (SD) (n = 4). (D) IFN-g secretion was quantified in coculture supernatants by ELISA to evaluate MHC-I Ag pre- sentation by IL-4, IL-21, Combo-CD40B cells, or MoDC pulsed with indicated peptide concentra- tions. T cells only or T cells stimulated with PMA/ ionomycin were used as controls. IFN-g secretion was quantified in two technical replicates for each sample and condition. Results are represented as mean (SD) (n = 8). (E) Representative <t>ELISPOT</t> well images containing 5 104 T cells cocultured with autologous CEF-loaded IL-4, IL-21, Combo- CD40B cells, or MoDC. The number of IFN-g1 T cells (SFU) was quantified in two technical replicates for each sample and condition and is presented in the left panel. T cells only or T cells stimulated with PMA/ionomycin were used as controls. SFU (mean [SD]) are pooled from repeated experi- ments (n = 2) from one CEF-reactive donor and are representative of two CEF-reactive donors (Supplemental Fig. 3C). The mean spot size rep- resenting IFN-g secretion was quantified in two technical replicates for each sample and condi- tion. Results are represented as mean (SD) (n = 2 CEF-reactive donor). (F) Representative histo- grams of T cell proliferation in an MLR for the APC/T cell ratio of 1:4. T cell proliferation was assessed by evaluating CTV dilution by flow cytometry after 6 d of culture with indicated stim- ulator cells or controls. Unstimulated T cells or T cells stimulated with anti-CD3, anti-CD28, and IL-2 were used as controls. Division indexes were calculated as mentioned in the Material and Meth- ods. Results are represented as mean (SD) (n = 9). T cell proliferation was assessed in two technical replicates for each sample and condition. Statistical significance of normally distributed data (Shapir- oWilk test) compared with Combo-CD40B cells was calculated using one-way (E) or two-way (BD, F) ANOVA with post hoc Tukey HSD. *p < 0.05, **p < 0.01.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_5061/pm34135061/pm34135061__page11_image1.jpg)