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recombinant anti flotillin 1  (Danaher Inc)


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    Structured Review

    Danaher Inc recombinant anti flotillin 1
    Recombinant Anti Flotillin 1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 2105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab267479/Nitrocellulose+Transfer+Membrane-+-+10Membrane/pmc10070242-66-16-23
    Average 99 stars, based on 2105 article reviews
    recombinant anti flotillin 1 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models
    Article Snippet: .. The pharmacological agents used were 5 or 10 μ m CCCP (Merck, C2759) for 6 h or 24 h (also 1.25, 2.5, 5, 10, 20 and 40 μ m for dose-response treatments), and 100 n m MLi-2 (Tocris, 5756) for 48 h. The primary antibodies used for western blotting (WB) or for immunocytochemistry (ICC) were rabbit anti-LRRK2 (WB, 1:100; Abcam ab133474), rabbit anti-LRRK2 (phospho S1292; WB, 1:100; Abcam ab203181) mouse anti-MIRO1 (WB, 1:700; Merck, WH0055288M1), rabbit anti-RAB10 (WB, 1:1000; Cell Signaling Technology, 8127S), rabbit anti-RAB10 (phospho T73; WB, 1:250; Abcam, ab230261), rabbit anti-RAB12 (WB, 1:1000; Proteintech, 18 843-1-AP), rabbit anti-RAB12 (phospho S106; WB, 1:250; Abcam, ab256487), rabbit anti-RAB8A (phospho T72; WB, 1:100; Abcam, ab230260), rabbit anti-GFP (WB, 1:100; ThermoScientific A-11122), HRP-conjugated anti-β-actin (WB, 1:50 000, Biolegend, 643807), anti-phospho-ubiquitin Alexa Fluor-488 conjugate (phosphor Ser65; ICC, 1:500; Merck, ABS1513-I-AF488), mouse anti-TOM20 (ICC, 1:500; Santa Cruz Biotechnology, sc-17 764F-10), rat anti-TOMM20 (ICC, 1:250; Abcam, ab289670 EPR15581-39), chicken anti-MAP2 (ICC, 1:1000; Abcam, ab92434), rabbit anti-TH (ICC, 1:500; Millipore, AB152), rabbit anti-MIRO1 (ICC, 1:100; Merck, HPA010687) and rabbit anti-LRRK2 (ICC, 1:100; Antibodies Inc., 75-253S). .. Peroxidase-linked secondary antibodies used for WB were goat anti-mouse IgG (H + L)–HRP conjugate (1706516) and goat anti-rabbit IgG (H + L)–HRP conjugate (1706515) (BioRad).

    Article Title: Systematic compositional analysis of exosomal extracellular vesicles produced by cells undergoing apoptosis, necroptosis and ferroptosis
    Article Snippet: ML162 (Aobious, Gloucester, MA) was used at 20 μM. .. The following antibodies were used for immunoblot: Alix (ab76608, Abcam), TSG101 (C‐2, sc‐7964, Santa Cruz), CD63 (EPR21151, ab217345, Abcam), CD81 (EPR4244, ab109201, Abcam & D5O2Q, 10037, Cell Signalling), Flotillin‐1 (EPR6041, ab133497, Abcam), Histone H3b (D1H2, Cell Signalling), Calnexin (610523, BD Biosciences), Calreticulin (EPR3924, ab92516, Abcam), Bcl‐2‐HRP (C‐2, sc‐7382, Santa Cruz), LAMP1 (L1418, Sigma‐Aldrich), phospho‐RIPK3 (E7S1R, 91702S, Cell Signalling), RIPK3 (2283, ProSci Incorporated), phospho‐MLKL (D6E3G, 37333S, Cell Signalling), MLKL (3H1, MABC604, Millipore), cleaved Caspase‐8 (9429, Cell Signalling), Caspase‐3 (9662, Cell Signalling), Caspase‐7 (9492, Cell Signalling), PARP (46D11, 9532S, Cell Signalling) and cleaved PARP (9544S, Cell Signalling). .. HRP‐conjugated secondary antibodies were anti‐rabbit IgG, anti‐mouse IgG, or anti‐rat IgG (GE Healthcare, NA934, NA931, NA935, respectively, 1:5000).

    Immunocytochemistry:

    Article Title: Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models
    Article Snippet: .. The pharmacological agents used were 5 or 10 μ m CCCP (Merck, C2759) for 6 h or 24 h (also 1.25, 2.5, 5, 10, 20 and 40 μ m for dose-response treatments), and 100 n m MLi-2 (Tocris, 5756) for 48 h. The primary antibodies used for western blotting (WB) or for immunocytochemistry (ICC) were rabbit anti-LRRK2 (WB, 1:100; Abcam ab133474), rabbit anti-LRRK2 (phospho S1292; WB, 1:100; Abcam ab203181) mouse anti-MIRO1 (WB, 1:700; Merck, WH0055288M1), rabbit anti-RAB10 (WB, 1:1000; Cell Signaling Technology, 8127S), rabbit anti-RAB10 (phospho T73; WB, 1:250; Abcam, ab230261), rabbit anti-RAB12 (WB, 1:1000; Proteintech, 18 843-1-AP), rabbit anti-RAB12 (phospho S106; WB, 1:250; Abcam, ab256487), rabbit anti-RAB8A (phospho T72; WB, 1:100; Abcam, ab230260), rabbit anti-GFP (WB, 1:100; ThermoScientific A-11122), HRP-conjugated anti-β-actin (WB, 1:50 000, Biolegend, 643807), anti-phospho-ubiquitin Alexa Fluor-488 conjugate (phosphor Ser65; ICC, 1:500; Merck, ABS1513-I-AF488), mouse anti-TOM20 (ICC, 1:500; Santa Cruz Biotechnology, sc-17 764F-10), rat anti-TOMM20 (ICC, 1:250; Abcam, ab289670 EPR15581-39), chicken anti-MAP2 (ICC, 1:1000; Abcam, ab92434), rabbit anti-TH (ICC, 1:500; Millipore, AB152), rabbit anti-MIRO1 (ICC, 1:100; Merck, HPA010687) and rabbit anti-LRRK2 (ICC, 1:100; Antibodies Inc., 75-253S). .. Peroxidase-linked secondary antibodies used for WB were goat anti-mouse IgG (H + L)–HRP conjugate (1706516) and goat anti-rabbit IgG (H + L)–HRP conjugate (1706515) (BioRad).

    Membrane:

    Article Title: Interleukin 31 receptor α promotes smooth muscle cell contraction and airway hyperresponsiveness in asthma
    Article Snippet: .. After SDS–PAGE separation, proteins were transferred to nitrocellulose membrane and incubated overnight with primary antibodies against CHRM3 (1:1000, Abcam), phosphoMLC (1:1000, Cell Signaling Technology), totalMLC (1:1000, Cell Signaling Technology), hIL-31RA (1:100, R&D Systems), ITGB1 (1:1000, Cell Signaling Technology) and GAPDH (1:2000, Bethyl Laboratories) and followed by detection with HRP conjugated secondary antibodies. .. Proteins were quantified by normalizing to GAPDH levels using the volume integration function of the BIORAD imager software.

    Article Title: Iron oxide nanoparticles induce ferroptosis via the autophagic pathway by synergistic bundling with paclitaxel
    Article Snippet: The pretreated NCI-H446 cells and M059K cells were separately collected to extract the protein from the cell lysate following 15 min of centrifugation at 21,912 × g at 4°C, and the protein concentration was determined using a BCA protein content detection kit (cat. no. KGA902; Nanjing KeyGen Biotech Co., Ltd.). .. First, identical amounts of protein (20 μg/lane) were separated on 10% gels using SDS-PAGE, transferred to PVDF membranes and blocked with fresh 5% nonfat dry milk at room temperature for 2 h. The membrane was incubated with diluted primary antibodies, including rabbit anti-P62 (1:1,000; cat. no. ab91526; Abcam), Nrf2 (1:5,000; cat. no. ab62352; Abcam), GPX4 (1:2,000; cat. no. ab123066; Abcam), mammalian target of rapamycin complex 1 (mTORC1) (1:2,000; cat. no. ab40768; Abcam), LC3II/I (1:2,000; cat. no. ab192890; Abcam), Beclin1 (1:1,000; cat. no. ab62557; Abcam), HDAC6 (1:10,000; cat. no. ab133493; Abcam), and GAPDH (1:5,000; cat. no. KGAA002; Nanjing KeyGen Biotech Co., Ltd.). .. After washing with Tris-buffered saline-Tween (TBST) three times, the membrane was subsequently incubated with the Goat Anti-Rabbit IgG/HRP antibody (1:10,000; cat. no. KGAA002; Nanjing KeyGen Biotech Co., Ltd.) for 1 h at room temperature.

    Incubation:

    Article Title: Interleukin 31 receptor α promotes smooth muscle cell contraction and airway hyperresponsiveness in asthma
    Article Snippet: .. After SDS–PAGE separation, proteins were transferred to nitrocellulose membrane and incubated overnight with primary antibodies against CHRM3 (1:1000, Abcam), phosphoMLC (1:1000, Cell Signaling Technology), totalMLC (1:1000, Cell Signaling Technology), hIL-31RA (1:100, R&D Systems), ITGB1 (1:1000, Cell Signaling Technology) and GAPDH (1:2000, Bethyl Laboratories) and followed by detection with HRP conjugated secondary antibodies. .. Proteins were quantified by normalizing to GAPDH levels using the volume integration function of the BIORAD imager software.

    Article Title: Iron oxide nanoparticles induce ferroptosis via the autophagic pathway by synergistic bundling with paclitaxel
    Article Snippet: The pretreated NCI-H446 cells and M059K cells were separately collected to extract the protein from the cell lysate following 15 min of centrifugation at 21,912 × g at 4°C, and the protein concentration was determined using a BCA protein content detection kit (cat. no. KGA902; Nanjing KeyGen Biotech Co., Ltd.). .. First, identical amounts of protein (20 μg/lane) were separated on 10% gels using SDS-PAGE, transferred to PVDF membranes and blocked with fresh 5% nonfat dry milk at room temperature for 2 h. The membrane was incubated with diluted primary antibodies, including rabbit anti-P62 (1:1,000; cat. no. ab91526; Abcam), Nrf2 (1:5,000; cat. no. ab62352; Abcam), GPX4 (1:2,000; cat. no. ab123066; Abcam), mammalian target of rapamycin complex 1 (mTORC1) (1:2,000; cat. no. ab40768; Abcam), LC3II/I (1:2,000; cat. no. ab192890; Abcam), Beclin1 (1:1,000; cat. no. ab62557; Abcam), HDAC6 (1:10,000; cat. no. ab133493; Abcam), and GAPDH (1:5,000; cat. no. KGAA002; Nanjing KeyGen Biotech Co., Ltd.). .. After washing with Tris-buffered saline-Tween (TBST) three times, the membrane was subsequently incubated with the Goat Anti-Rabbit IgG/HRP antibody (1:10,000; cat. no. KGAA002; Nanjing KeyGen Biotech Co., Ltd.) for 1 h at room temperature.

    other:




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    Image Search Results


    Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, CD9, and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm

    Journal: Journal of Nanobiotechnology

    Article Title: Vascular wall microenvironment: exosomes secreted by adventitial fibroblasts induced vascular calcification

    doi: 10.1186/s12951-023-02000-3

    Figure Lengend Snippet: Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, CD9, and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm

    Article Snippet: After blocking with 5% non-fat milk for 1 h, the membrane was incubated overnight at 4 °C with primary antibody, including CD9 (ab92726, 1:1000, abcam), CD81 (ab109201, 1:1000, abcam), TSG101 (bs-1365R, 1:1000, bioss), Runx2 (ab23981, 1:2000, abcam), BMP2 (bs-10696R, 1:1000, bioss), GAPDH (10494-1-AP, 1:4000, proteintech), BMP4 (bs-1374R, 1:1000, bioss), Crim1 (bs-21654R, 1:1000, bioss), followed by incubation with the horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature.

    Techniques: Derivative Assay, Transmission Assay, Western Blot, Incubation, Staining

    Figure 1. Characteristics of SVF-EVs. (A) TEM images of SVF-EVs. (B) NTA analysis of SVF-EVs. The confirmed size distribution with a range of diameter around 100–300 nm. (C) Western blot shows that SVF- EVs express tetraspanin proteins like TSG101 and CD81. (D) Western blot reveals that SVF-EVs encapsulate proteins such as PPARγ, C/EBPα, Ang-1, and VEGF. (E) Confocal images verify the internalization of PKH26- labeled SVF-EVs (red) into endothelial cell (CD31-labeled, green), and the amount of endocytosed SVF-EVs after co-culturing for 4 h is more than that for 2 h (F).

    Journal: Scientific reports

    Article Title: Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors.

    doi: 10.1038/s41598-022-27176-w

    Figure Lengend Snippet: Figure 1. Characteristics of SVF-EVs. (A) TEM images of SVF-EVs. (B) NTA analysis of SVF-EVs. The confirmed size distribution with a range of diameter around 100–300 nm. (C) Western blot shows that SVF- EVs express tetraspanin proteins like TSG101 and CD81. (D) Western blot reveals that SVF-EVs encapsulate proteins such as PPARγ, C/EBPα, Ang-1, and VEGF. (E) Confocal images verify the internalization of PKH26- labeled SVF-EVs (red) into endothelial cell (CD31-labeled, green), and the amount of endocytosed SVF-EVs after co-culturing for 4 h is more than that for 2 h (F).

    Article Snippet: Then membranes were blocked with 5% skimmed milk, and incubated with specific antibodies against TSG101 (ab133586, Abcam, UK), CD81(ab109201, Abcam, UK), PPARγ (sc-7273, Santa, USA), CEBPα (sc-365318, Santa, USA), Ang-1 (23302-1-AP, Sanying, China) and VEGF (66828-1-Ig, Sanying, China) overnight at 4 °C.

    Techniques: Western Blot, Labeling