recombinant anti flotillin 1 (Danaher Inc)
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Recombinant Anti Flotillin 1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 2105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab267479/Nitrocellulose+Transfer+Membrane-+-+10Membrane/pmc10070242-66-16-23
Average 99 stars, based on 2105 article reviews
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Western Blot:Article Title: Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models Article Snippet: .. The pharmacological agents used were 5 or 10 μ m CCCP (Merck, C2759) for 6 h or 24 h (also 1.25, 2.5, 5, 10, 20 and 40 μ m for dose-response treatments), and 100 n m MLi-2 (Tocris, 5756) for 48 h. The primary antibodies used for western blotting (WB) or for immunocytochemistry (ICC) were Article Title: Systematic compositional analysis of exosomal extracellular vesicles produced by cells undergoing apoptosis, necroptosis and ferroptosis Article Snippet: ML162 (Aobious, Gloucester, MA) was used at 20 μM. .. The following antibodies were used for immunoblot: Alix (ab76608, Abcam), TSG101 (C‐2, sc‐7964, Santa Cruz), CD63 (EPR21151, ab217345, Abcam), CD81 (EPR4244, ab109201, Abcam & D5O2Q, 10037, Cell Signalling), Immunocytochemistry:Article Title: Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models Article Snippet: .. The pharmacological agents used were 5 or 10 μ m CCCP (Merck, C2759) for 6 h or 24 h (also 1.25, 2.5, 5, 10, 20 and 40 μ m for dose-response treatments), and 100 n m MLi-2 (Tocris, 5756) for 48 h. The primary antibodies used for western blotting (WB) or for immunocytochemistry (ICC) were Membrane:Article Title: Interleukin 31 receptor α promotes smooth muscle cell contraction and airway hyperresponsiveness in asthma Article Snippet: .. After SDS–PAGE separation, proteins were transferred to Article Title: Iron oxide nanoparticles induce ferroptosis via the autophagic pathway by synergistic bundling with paclitaxel Article Snippet: The pretreated NCI-H446 cells and M059K cells were separately collected to extract the protein from the cell lysate following 15 min of centrifugation at 21,912 × g at 4°C, and the protein concentration was determined using a BCA protein content detection kit (cat. no. KGA902; Nanjing KeyGen Biotech Co., Ltd.). .. First, identical amounts of protein (20 μg/lane) were separated on 10% gels using SDS-PAGE, transferred to PVDF membranes and blocked with fresh 5% nonfat dry milk at room temperature for 2 h. The membrane was incubated with diluted primary antibodies, including rabbit anti-P62 (1:1,000; cat. no. ab91526; Abcam), Nrf2 (1:5,000; cat. no. ab62352; Abcam), GPX4 (1:2,000; cat. no. ab123066; Abcam), mammalian target of rapamycin complex 1 (mTORC1) (1:2,000; cat. no. ab40768; Abcam), LC3II/I (1:2,000; cat. no. ab192890; Abcam), Beclin1 (1:1,000; cat. no. ab62557; Abcam), Incubation:Article Title: Interleukin 31 receptor α promotes smooth muscle cell contraction and airway hyperresponsiveness in asthma Article Snippet: .. After SDS–PAGE separation, proteins were transferred to Article Title: Iron oxide nanoparticles induce ferroptosis via the autophagic pathway by synergistic bundling with paclitaxel Article Snippet: The pretreated NCI-H446 cells and M059K cells were separately collected to extract the protein from the cell lysate following 15 min of centrifugation at 21,912 × g at 4°C, and the protein concentration was determined using a BCA protein content detection kit (cat. no. KGA902; Nanjing KeyGen Biotech Co., Ltd.). .. First, identical amounts of protein (20 μg/lane) were separated on 10% gels using SDS-PAGE, transferred to PVDF membranes and blocked with fresh 5% nonfat dry milk at room temperature for 2 h. The membrane was incubated with diluted primary antibodies, including rabbit anti-P62 (1:1,000; cat. no. ab91526; Abcam), Nrf2 (1:5,000; cat. no. ab62352; Abcam), GPX4 (1:2,000; cat. no. ab123066; Abcam), mammalian target of rapamycin complex 1 (mTORC1) (1:2,000; cat. no. ab40768; Abcam), LC3II/I (1:2,000; cat. no. ab192890; Abcam), Beclin1 (1:1,000; cat. no. ab62557; Abcam), other:Article Title: Identification of Bone Morphometric Protein-Related Hub Genes and Construction of a Transcriptional Regulatory Network in Patients With Ossification of the Ligamentum Flavum Article Snippet: |

