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antibodies anticd9  (Danaher Inc)


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    Structured Review

    Danaher Inc antibodies anticd9
    Antibodies Anticd9, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 757 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab267479/anti-NCAM1+antibody/10__4314_slash_tjpr__v22i3__8-46-1-12
    Average 99 stars, based on 757 article reviews
    antibodies anticd9 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: Multiple synchronous primary malignant neoplasms: A case report and literature review
    Article Snippet: Anti-NCAM1 antibody , Abcam , ab133345 , 1:500.

    Saline:

    Article Title: Circulating small extracellular vesicle-derived splicing factor 3b subunit 4 as a non-invasive diagnostic biomarker of early hepatocellular carcinoma
    Article Snippet: Subsequently, 10 μg protein was separated on a 4–20% Mini-PROTEAN TGXTM gel (Bio-Rad Laboratories, Hercules, CA, USA) and transferred onto polyvinylidene difluoride membranes (Amersham; GE Healthcare, Munich, Germany). .. The membranes were blocked using 5% non-fat milk in Tris-Buffer Saline (TBS) and 0.1% Tween-20 and immunoblotted utilizing the following primary antibodies: mouse anti-CD63 (1:1000, ab134045; Abcam, Cambridge, MA, USA), mouse anti-CD81 (1:250, 10630D; Invitrogen, Carlsbad, CA, USA), rabbit anti-CD9 (1:2000, ab92726; Abcam), and mouse anti-BiP/GRP78 (1:1000, 610979; BD Biosciences, San Jose, CA, USA). .. The samples were then incubated with secondary HRP-conjugated anti-rabbit (BR170-6515; Bio-Rad Laboratories) or anti-mouse (BR170-6516; Bio-Rad Laboratories) antibodies.

    Western Blot:

    Article Title: Neuronal MHC-I complex is destabilized by amyloid-β and its implications in Alzheimer’s disease
    Article Snippet: The blots were incubated with primary antibodies overnight at 4 °C, washed three times in PBS-T buffer, and incubated with horseradish peroxidase-conjugated secondary antibodies for 1 h. The immunoblots were visualized using ECL reagents (Thermo Fisher Scientific Inc.). .. The primary antibodies used in the western blotting analyses were anti-human MHC-I (NBP2-66946, Novus Biologicals, LLC, Littleton, CO, USA); anti-β 2 M (Abcam plc, Cambridge, UK); anti-NCAM1 (14255-1-AP, Proteintech Group, Inc., Rosemont, IL, USA); anti-β-actin (MilliporeSigma); anti-protein disulfide isomerase (Thermo Fisher Scientific, Inc.); anti-mouse MHC-I (OX-18; Abcam plc); anti-PSD95, anti-synaptophysin, anti-glyceraldehyde 3-phosphate dehydrogenase (MilliporeSigma); and anti-ERp57, anti-tapasin, anti-TAP, anti-pCREB, anti-CREB, anti-c-fos, and anti-BDNF (Santa Cruz Biotechnology, Inc., Dallas, TX, USA). .. The band intensities were measured and analyzed with ImageJ software ( https://imagej.nih.gov/ij/ ).

    Immunopeptidomics:

    Article Title: Neuronal MHC-I complex is destabilized by amyloid-β and its implications in Alzheimer’s disease
    Article Snippet: The blots were incubated with primary antibodies overnight at 4 °C, washed three times in PBS-T buffer, and incubated with horseradish peroxidase-conjugated secondary antibodies for 1 h. The immunoblots were visualized using ECL reagents (Thermo Fisher Scientific Inc.). .. The primary antibodies used in the western blotting analyses were anti-human MHC-I (NBP2-66946, Novus Biologicals, LLC, Littleton, CO, USA); anti-β 2 M (Abcam plc, Cambridge, UK); anti-NCAM1 (14255-1-AP, Proteintech Group, Inc., Rosemont, IL, USA); anti-β-actin (MilliporeSigma); anti-protein disulfide isomerase (Thermo Fisher Scientific, Inc.); anti-mouse MHC-I (OX-18; Abcam plc); anti-PSD95, anti-synaptophysin, anti-glyceraldehyde 3-phosphate dehydrogenase (MilliporeSigma); and anti-ERp57, anti-tapasin, anti-TAP, anti-pCREB, anti-CREB, anti-c-fos, and anti-BDNF (Santa Cruz Biotechnology, Inc., Dallas, TX, USA). .. The band intensities were measured and analyzed with ImageJ software ( https://imagej.nih.gov/ij/ ).

    Article Title: Neuronal MHC-I complex is destabilized by amyloid-β and its implications in Alzheimer’s disease
    Article Snippet: .. To assess the co-localization of MHC-I and NCAM1 or MHC-I and β 2 M on primary neurons in the presence or absence of Aβ oligomer treatment, the cells were fixed and permeabilized with PBS containing 0.5% Triton X-100 for 10 min and incubated with blocking buffer (10% NGS, 5% bovine serum albumin [BSA], and 0.5% Tween 20 in PBS) for 1 h. The cells were subsequently incubated with primary anti-mouse MHC-I(B22-249.R1, Thermo Fisher Scientific Inc), anti-NCAM1(Proteintech) and anti-β 2 M(Abcam) antibodies overnight at 4 °C, washed three times with PBS, and incubated with secondary Alexa fluor 488/594-conjugated anti-mouse or anti-rabbit antibody for 1 h at RT. .. The cells were again washed three times with PBS, stained with Hoechst 33,342 (Thermo Fisher Scientific Inc.) for 10 min, again washed with PBS, and mounted onto slides.

    Bicinchoninic Acid Protein Assay:

    Article Title: Lipid metabolism of plasma-derived small extracellular vesicles in COVID-19 convalescent patients
    Article Snippet: .. Protein concentrations were detected with BCA Protein Assay according to the manufacturer’s protocols. sEVs markers CD9 (Abcam, ab92726), CD63 (Abcam, ab193349), TSG101 (Abcam, ab83), and sEVs non-markers calnexin (Abcam, ab22595), GM130 (Abcam, ab52649), Lamin B1 (Abcam, ab16048), and GRP94 (Proteintech, 14700-1-AP) were measured to validate the expressions of sEVs as described in prior studies , . .. The lipidomics of sEVs samples was performed by Metabo-Profile (Shanghai, China).

    Incubation:

    Article Title: Neuronal MHC-I complex is destabilized by amyloid-β and its implications in Alzheimer’s disease
    Article Snippet: .. To assess the co-localization of MHC-I and NCAM1 or MHC-I and β 2 M on primary neurons in the presence or absence of Aβ oligomer treatment, the cells were fixed and permeabilized with PBS containing 0.5% Triton X-100 for 10 min and incubated with blocking buffer (10% NGS, 5% bovine serum albumin [BSA], and 0.5% Tween 20 in PBS) for 1 h. The cells were subsequently incubated with primary anti-mouse MHC-I(B22-249.R1, Thermo Fisher Scientific Inc), anti-NCAM1(Proteintech) and anti-β 2 M(Abcam) antibodies overnight at 4 °C, washed three times with PBS, and incubated with secondary Alexa fluor 488/594-conjugated anti-mouse or anti-rabbit antibody for 1 h at RT. .. The cells were again washed three times with PBS, stained with Hoechst 33,342 (Thermo Fisher Scientific Inc.) for 10 min, again washed with PBS, and mounted onto slides.

    Blocking Assay:

    Article Title: Neuronal MHC-I complex is destabilized by amyloid-β and its implications in Alzheimer’s disease
    Article Snippet: .. To assess the co-localization of MHC-I and NCAM1 or MHC-I and β 2 M on primary neurons in the presence or absence of Aβ oligomer treatment, the cells were fixed and permeabilized with PBS containing 0.5% Triton X-100 for 10 min and incubated with blocking buffer (10% NGS, 5% bovine serum albumin [BSA], and 0.5% Tween 20 in PBS) for 1 h. The cells were subsequently incubated with primary anti-mouse MHC-I(B22-249.R1, Thermo Fisher Scientific Inc), anti-NCAM1(Proteintech) and anti-β 2 M(Abcam) antibodies overnight at 4 °C, washed three times with PBS, and incubated with secondary Alexa fluor 488/594-conjugated anti-mouse or anti-rabbit antibody for 1 h at RT. .. The cells were again washed three times with PBS, stained with Hoechst 33,342 (Thermo Fisher Scientific Inc.) for 10 min, again washed with PBS, and mounted onto slides.

    Next-Generation Sequencing:

    Article Title: Neuronal MHC-I complex is destabilized by amyloid-β and its implications in Alzheimer’s disease
    Article Snippet: .. To assess the co-localization of MHC-I and NCAM1 or MHC-I and β 2 M on primary neurons in the presence or absence of Aβ oligomer treatment, the cells were fixed and permeabilized with PBS containing 0.5% Triton X-100 for 10 min and incubated with blocking buffer (10% NGS, 5% bovine serum albumin [BSA], and 0.5% Tween 20 in PBS) for 1 h. The cells were subsequently incubated with primary anti-mouse MHC-I(B22-249.R1, Thermo Fisher Scientific Inc), anti-NCAM1(Proteintech) and anti-β 2 M(Abcam) antibodies overnight at 4 °C, washed three times with PBS, and incubated with secondary Alexa fluor 488/594-conjugated anti-mouse or anti-rabbit antibody for 1 h at RT. .. The cells were again washed three times with PBS, stained with Hoechst 33,342 (Thermo Fisher Scientific Inc.) for 10 min, again washed with PBS, and mounted onto slides.



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    Image Search Results


    Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, CD9, and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm

    Journal: Journal of Nanobiotechnology

    Article Title: Vascular wall microenvironment: exosomes secreted by adventitial fibroblasts induced vascular calcification

    doi: 10.1186/s12951-023-02000-3

    Figure Lengend Snippet: Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFs NPi -Exos and AFs HPi -Exos. The scale bar represents 200 μm. B The diameter distribution of AFs NPi -Exos and AFs HPi -Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, CD9, and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFs NPi -Exos, AFs HPi -Exos). D Representative images of AFs NPi -Exos and AFs HPi -Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm

    Article Snippet: After blocking with 5% non-fat milk for 1 h, the membrane was incubated overnight at 4 °C with primary antibody, including CD9 (ab92726, 1:1000, abcam), CD81 (ab109201, 1:1000, abcam), TSG101 (bs-1365R, 1:1000, bioss), Runx2 (ab23981, 1:2000, abcam), BMP2 (bs-10696R, 1:1000, bioss), GAPDH (10494-1-AP, 1:4000, proteintech), BMP4 (bs-1374R, 1:1000, bioss), Crim1 (bs-21654R, 1:1000, bioss), followed by incubation with the horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature.

    Techniques: Derivative Assay, Transmission Assay, Western Blot, Incubation, Staining

    Figure 1. Characteristics of SVF-EVs. (A) TEM images of SVF-EVs. (B) NTA analysis of SVF-EVs. The confirmed size distribution with a range of diameter around 100–300 nm. (C) Western blot shows that SVF- EVs express tetraspanin proteins like TSG101 and CD81. (D) Western blot reveals that SVF-EVs encapsulate proteins such as PPARγ, C/EBPα, Ang-1, and VEGF. (E) Confocal images verify the internalization of PKH26- labeled SVF-EVs (red) into endothelial cell (CD31-labeled, green), and the amount of endocytosed SVF-EVs after co-culturing for 4 h is more than that for 2 h (F).

    Journal: Scientific reports

    Article Title: Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors.

    doi: 10.1038/s41598-022-27176-w

    Figure Lengend Snippet: Figure 1. Characteristics of SVF-EVs. (A) TEM images of SVF-EVs. (B) NTA analysis of SVF-EVs. The confirmed size distribution with a range of diameter around 100–300 nm. (C) Western blot shows that SVF- EVs express tetraspanin proteins like TSG101 and CD81. (D) Western blot reveals that SVF-EVs encapsulate proteins such as PPARγ, C/EBPα, Ang-1, and VEGF. (E) Confocal images verify the internalization of PKH26- labeled SVF-EVs (red) into endothelial cell (CD31-labeled, green), and the amount of endocytosed SVF-EVs after co-culturing for 4 h is more than that for 2 h (F).

    Article Snippet: Then membranes were blocked with 5% skimmed milk, and incubated with specific antibodies against TSG101 (ab133586, Abcam, UK), CD81(ab109201, Abcam, UK), PPARγ (sc-7273, Santa, USA), CEBPα (sc-365318, Santa, USA), Ang-1 (23302-1-AP, Sanying, China) and VEGF (66828-1-Ig, Sanying, China) overnight at 4 °C.

    Techniques: Western Blot, Labeling