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picrosirius red solution  (Danaher Inc)


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    Danaher Inc picrosirius red solution
    Apabetalone blocks TGF-β1-induced HRMC activation. ( A ): HRMCs were treated with TGF-β1 ± BETi or TGFBRi for 24 h followed by gene expression analysis by real-time PCR ( n = 5). ( B ): Representative images of HRMCs treated with TGF-β1 ± apabetalone for 48 h, followed by immunofluorescence microscopy for α-SMA (green); nuclei were stained with DAPI (blue); apa = apabetalone. ( C ): Fluorescence intensity of α-SMA was quantified as percent of the image area. ( D ): Collagen gel contraction was evaluated after 4 days of treatment ( n = 6). ( E ) Collagen deposition was evaluated by <t>picrosirius</t> red staining after 5 days of treatment ( n = 4). Data in bar graphs are the mean ± SD. Statistical analysis by one-way ANOVA followed by Dunnett’s Multiple Comparison Test. *** p < 0.001, ** p < 0.01, NS not significant. ACTA2: α-SMA gene. α-SMA: alpha smooth muscle actin. TGF-β1: Transforming growth factor β1. Apabetalone: BD2-selective BET inhibitor. JQ1: pan-BET inhibitor. MZ1: PROTAC that directs BET proteins for degradation. TGFBRi: small molecule inhibitor of the TGF-β receptor.
    Picrosirius Red Solution, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 312 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab246832/Picro-Sirius+Red+Solution/pmc10295623-93-4-7
    Average 99 stars, based on 312 article reviews
    picrosirius red solution - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Apabetalone Downregulates Fibrotic, Inflammatory and Calcific Processes in Renal Mesangial Cells and Patients with Renal Impairment"

    Article Title: Apabetalone Downregulates Fibrotic, Inflammatory and Calcific Processes in Renal Mesangial Cells and Patients with Renal Impairment

    Journal: Biomedicines

    doi: 10.3390/biomedicines11061663

    Apabetalone blocks TGF-β1-induced HRMC activation. ( A ): HRMCs were treated with TGF-β1 ± BETi or TGFBRi for 24 h followed by gene expression analysis by real-time PCR ( n = 5). ( B ): Representative images of HRMCs treated with TGF-β1 ± apabetalone for 48 h, followed by immunofluorescence microscopy for α-SMA (green); nuclei were stained with DAPI (blue); apa = apabetalone. ( C ): Fluorescence intensity of α-SMA was quantified as percent of the image area. ( D ): Collagen gel contraction was evaluated after 4 days of treatment ( n = 6). ( E ) Collagen deposition was evaluated by picrosirius red staining after 5 days of treatment ( n = 4). Data in bar graphs are the mean ± SD. Statistical analysis by one-way ANOVA followed by Dunnett’s Multiple Comparison Test. *** p < 0.001, ** p < 0.01, NS not significant. ACTA2: α-SMA gene. α-SMA: alpha smooth muscle actin. TGF-β1: Transforming growth factor β1. Apabetalone: BD2-selective BET inhibitor. JQ1: pan-BET inhibitor. MZ1: PROTAC that directs BET proteins for degradation. TGFBRi: small molecule inhibitor of the TGF-β receptor.
    Figure Legend Snippet: Apabetalone blocks TGF-β1-induced HRMC activation. ( A ): HRMCs were treated with TGF-β1 ± BETi or TGFBRi for 24 h followed by gene expression analysis by real-time PCR ( n = 5). ( B ): Representative images of HRMCs treated with TGF-β1 ± apabetalone for 48 h, followed by immunofluorescence microscopy for α-SMA (green); nuclei were stained with DAPI (blue); apa = apabetalone. ( C ): Fluorescence intensity of α-SMA was quantified as percent of the image area. ( D ): Collagen gel contraction was evaluated after 4 days of treatment ( n = 6). ( E ) Collagen deposition was evaluated by picrosirius red staining after 5 days of treatment ( n = 4). Data in bar graphs are the mean ± SD. Statistical analysis by one-way ANOVA followed by Dunnett’s Multiple Comparison Test. *** p < 0.001, ** p < 0.01, NS not significant. ACTA2: α-SMA gene. α-SMA: alpha smooth muscle actin. TGF-β1: Transforming growth factor β1. Apabetalone: BD2-selective BET inhibitor. JQ1: pan-BET inhibitor. MZ1: PROTAC that directs BET proteins for degradation. TGFBRi: small molecule inhibitor of the TGF-β receptor.

    Techniques Used: Activation Assay, Gene Expression, Real-time Polymerase Chain Reaction, Immunofluorescence, Microscopy, Staining, Fluorescence, Comparison

    Related Articles

    Staining:

    Article Title: Evolution of hypoxia and hypoxia-inducible factor asparaginyl hydroxylase regulation in chronic kidney disease
    Article Snippet: .. Briefly, a median section of the kidney was selected and stained with Sirius Red solution (Abcam, Cambridge, UK), according to the manufacturer's protocol. .. The slides were scanned with an Axioscan image scanner (Zeiss, Oberkochen, Germany) at 20× magnification.

    Article Title: Cardiomyocyte NOX4 regulates resident macrophage-mediated inflammation and diastolic dysfunction in stress cardiomyopathy
    Article Snippet: .. Frozen heart sections were stained with picro-sirius red using Connective Tissue Stain Kit (Abcam; Waltham, MA) and quantified as previously described [ ]. ..

    Article Title: Cardiomyocyte-specific Loss of Glutamyl-prolyl-tRNA Synthetase Leads to Disturbed Protein Homeostasis and Dilated Cardiomyopathy
    Article Snippet: .. Picrosirius staining was performed to measure cardiac fibrosis in the hearts using Picrosirius Red Solution (Abcam) following the manufacturer’s instruction. ..

    Article Title: Ectopic adipogenesis in response to injury and material implantation in an autoimmune mouse model
    Article Snippet: Quadriceps muscles were removed from the bone and fixed in 10% neutral buffered formalin (Sigma) for two days, rinsed in distilled water and dehydrated in a graded ethanol series before clearing in xylenes and infiltrating with paraffin wax (Leica). .. The resulting formalin fixed paraffin embedded (FFPE) samples were mounted on paraffin blocks and sectioned into 5 μm sections then stained with hematoxylin and eosin (H&E, Sigma) or picrosirius red (PSR, Abcam) using standard protocols as per manufacturer’s instructions. ..

    Article Title: A 4D transcriptomic map for the evolution of multiple sclerosis-like lesions in the marmoset brain
    Article Snippet: After removing excess SB solution, stained slide was dipped in 50 mL of ddH 2 O (351-029-131CS, Quality Biological) in a falcon tube for 5 times, dipped in 800 mL of ddH 2 O 15 times, and another 15 times in a separate glass beaker containing 800 mL of ddH 2 O. .. To get an optimal contrast between myelin and gray matter, tissue was dried for 1 min (the time should be extended if the tissue section is not dry) before being stained with 1 mL Nuclear Fast Red (NFR, ab246831, Abcam) for 10 minutes at RT at a leveled surface and rinsed again as described for SB staining. .. To avoid bubbles, slides were first saturated with 5 mL of ddH 2 O on a leveled surface and gradually replaced with 80% glycerol solution containing 5% RNase inhibitor (AM2684, Thermo Fisher Scientific) through steady vacuuming off ddH 2 O from one end of the slide and adding glycerol with a pipette at the opposite end simultaneously.

    Article Title: Lineage Plasticity in SCLC Generates Non-Neuroendocrine Cells Primed for Vasculogenic Mimicry
    Article Snippet: .. For collagen assessment by picrosirius red (PSR) staining, cells were incubated for 1 hour at room temperature with PSR staining solution before rinsing twice in acetic acid solution (picrosirius red stain kit, Abcam, ab150681) and imaging. .. For glycoprotein assessment, cells were incubated for 5 minutes in 0.5% periodic acid (Sigma-Aldrich, 375810), rinsing wells twice in PBS, staining with Schiff’s fushin-sulfite reagent (Sigma-Aldrich, S5133) for 15 minutes, rinsing extensively in PBS, and imaging by light microscopy.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Ectopic adipogenesis in response to injury and material implantation in an autoimmune mouse model
    Article Snippet: Quadriceps muscles were removed from the bone and fixed in 10% neutral buffered formalin (Sigma) for two days, rinsed in distilled water and dehydrated in a graded ethanol series before clearing in xylenes and infiltrating with paraffin wax (Leica). .. The resulting formalin fixed paraffin embedded (FFPE) samples were mounted on paraffin blocks and sectioned into 5 μm sections then stained with hematoxylin and eosin (H&E, Sigma) or picrosirius red (PSR, Abcam) using standard protocols as per manufacturer’s instructions. ..

    Incubation:

    Article Title: Lineage Plasticity in SCLC Generates Non-Neuroendocrine Cells Primed for Vasculogenic Mimicry
    Article Snippet: .. For collagen assessment by picrosirius red (PSR) staining, cells were incubated for 1 hour at room temperature with PSR staining solution before rinsing twice in acetic acid solution (picrosirius red stain kit, Abcam, ab150681) and imaging. .. For glycoprotein assessment, cells were incubated for 5 minutes in 0.5% periodic acid (Sigma-Aldrich, 375810), rinsing wells twice in PBS, staining with Schiff’s fushin-sulfite reagent (Sigma-Aldrich, S5133) for 15 minutes, rinsing extensively in PBS, and imaging by light microscopy.

    Imaging:

    Article Title: Lineage Plasticity in SCLC Generates Non-Neuroendocrine Cells Primed for Vasculogenic Mimicry
    Article Snippet: .. For collagen assessment by picrosirius red (PSR) staining, cells were incubated for 1 hour at room temperature with PSR staining solution before rinsing twice in acetic acid solution (picrosirius red stain kit, Abcam, ab150681) and imaging. .. For glycoprotein assessment, cells were incubated for 5 minutes in 0.5% periodic acid (Sigma-Aldrich, 375810), rinsing wells twice in PBS, staining with Schiff’s fushin-sulfite reagent (Sigma-Aldrich, S5133) for 15 minutes, rinsing extensively in PBS, and imaging by light microscopy.



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