hif1α transcription factor assay kit (Danaher Inc)
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hif1α transcription factor assay kit
Hif1α Transcription Factor Assay Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab235649/LDL+Uptake+Assay+Kit+(Cell-Based)+1+Kit/pmc10197896-75-2-8
Average 99 stars, based on 295 article reviews
Hif1α Transcription Factor Assay Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab235649/LDL+Uptake+Assay+Kit+(Cell-Based)+1+Kit/pmc10197896-75-2-8
Average 99 stars, based on 295 article reviews
hif1α transcription factor assay kit - by Bioz Stars,
2026-09
99/100 stars
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1) Product Images from "ACE2 negatively regulates the Warburg effect and suppresses hepatocellular carcinoma progression via reducing ROS-HIF1α activity"
Article Title: ACE2 negatively regulates the Warburg effect and suppresses hepatocellular carcinoma progression via reducing ROS-HIF1α activity
Journal: International Journal of Biological Sciences
doi: 10.7150/ijbs.81498
Figure Legend Snippet: ACE2 suppresses HIF1α activity in HCC. ( A ) GSEA plot showed a close link between ACE2 expression and hypoxia in HCC samples from the TCGA cohort. ( B ) The effects of ACE2 overexpression on HIF1α transcriptional activity in the presence or absence of A779 (1 μM) were measured in SNU-475 and SK-Hep1 cells. ( C ) The effects of ACE2 knockdown on HIF1α transcriptional activity in the presence or absence of Ang-(1-7) (10 -8 M) were measured in HCC-LM3 and Hep3B cells. ( D ) The effects of ACE2 overexpression on the activity of ACE2-associated signaling pathways (p38 MAPK, ERK1/2, JNK, NF-κB, AKT, SHP2, and HIF1α) in SNU-475 and SK-Hep1 cells was analyzed by Western blotting. ( E ) The levels of p-SHP2, SHP2, and HIF1α in sh-Ctrl and sh-ACE2 HCC-LM3 and Hep3B cells was analyzed by Western blotting. ( F ) The effects of ACE2 overexpression on ROS generation in the presence or absence of A779 (1 μM) were measured in SNU-475 and SK-Hep1 cells. ( G ) The effects of ACE2 knockdown on ROS generation in the presence or absence of Ang-(1-7) (10 -8 M) were measured in HCC-LM3 and Hep3B cells. ( H ) The effects of ACE2 knockdown on HIF1α transcriptional activity in the presence or absence of 1 mM N-acetyl cysteine (NAC) treatment were measured in HCC-LM3 and Hep3B cells. *P < 0.05 and **P < 0.01.
Techniques Used: Activity Assay, Expressing, Over Expression, Knockdown, Protein-Protein interactions, Western Blot
Figure Legend Snippet: Clinical relevance of ACE2 in HCC samples . ( A ) Representative IHC staining of ACE2, HIF1α or p-SHP2 protein levels in human HCC specimens (n = 202); scale bar, 50 μm. ( B ) Correlation between ACE2 and p-SHP2 protein was determined by Spearman analysis. ( C ) Correlation between ACE2 and HIF1αprotein was determined by Spearman analysis. ( D ) For PDX model, representative IHC staining of ACE2 in two HCC samples was shown. ( E ) The effect of ACE2 on the tumor growth of PDX xenograft was studied by a subcutaneous xenograft model. ( F ) The schematic diagram for the molecular mechanism of ACE2-mediated suppression of HIF1α activity and tumor growth in HCC. In brief, ACE2 metabolizes Ang II to Ang-(1-7), which activates Mas receptor and leads to the phosphorylation of SHP2. SHP2 activation blocks ROS generation, which further stabilizes HIF1α protein. HIF1α acts as a key transcriptional factor to induce the expression of glucose transporters and glycolytic genes and enhances the Warburg effect. Finally, tumor growth of HCC is suppressed by ACE2 due to compromised glycolytic flux. ***P < 0.001.
Techniques Used: Immunohistochemistry, Activity Assay, Phospho-proteomics, Activation Assay, Expressing
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