human ace2 simplestep elisa kit (Danaher Inc)
Danaher Inc is a verified supplier 
Danaher Inc manufactures this product 
99
Structured Review
Danaher Inc
human ace2 simplestep elisa kit
Human Ace2 Simplestep Elisa Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab235649/Human+ACE2+ELISA+Kit+1x96+test/bio_rxiv__2022__05__06__490907-181-11-17
Average 99 stars, based on 35 article reviews
Human Ace2 Simplestep Elisa Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab235649/Human+ACE2+ELISA+Kit+1x96+test/bio_rxiv__2022__05__06__490907-181-11-17
Average 99 stars, based on 35 article reviews
human ace2 simplestep elisa kit - by Bioz Stars,
2026-09
99/100 stars
Images
1) Product Images from "GDF15 and ACE2 stratify COVID19 patients according to severity while ACE2 mutations increase infection susceptibility"
Article Title: GDF15 and ACE2 stratify COVID19 patients according to severity while ACE2 mutations increase infection susceptibility
Journal: bioRxiv
doi: 10.1101/2022.05.06.490907
Figure Legend Snippet: A) GDF15 levels; B) ACE2 levels ; C) ratio GDf15/ACE2 ; The box plots represent the maximum and minimum levels (whiskers), the upper and lower quartiles, and the median. The length of each box represents the interquartile range. Dots represent outliers. Statistical significance between groups was determined using the ANOVA test. ** p <0.001, # p <0.001 UCI vs Control, n/s (non significant); GDF15 (Growth differentiation factor 15); ACE2 (angiotensin-converting enzyme 2).
Techniques Used: Control
Figure Legend Snippet: GDF15 is positive associated with age (A), while ACE2 is not correlated with age (B). Each dot represents an individual value. The solid blue line represents the regression line. The grey shade represents the confidence interval.
Techniques Used:
Figure Legend Snippet: The box plots represent the maximum and minimum levels (whiskers), the upper and lower quartiles, and the median. The length of each box represents the interquartile range. Dots represent outliers. Statistical significance between groups was determined using the ANOVA test. ** p <0.001, # p <0.001 UCI vs Control, n/s (non significant); ACE2 (angiotensin-converting enzyme 2).
Techniques Used: Control
Figure Legend Snippet:
Techniques Used:
Figure Legend Snippet: A) SARS-CoV-2 cell entry assay strategy: Lentiviral-based replication-defective pseudovirus were generated in HEK293T cells from lentiviral parental genes, SARS-CoV-2 Spike and encapsulating a mCherry reporter. Since the entry steps of the SARS-CoV-2 pseudovirions are governed by the coronavirus Spike protein at their surface, they enter cells in a similar fashion to native counterparts. A549 airway cells were transfected with exogenous GFP-hACE2 enabling SARS-CoV-2 pseudovirus to effectively infect the cells with mCherry reporter. Double-positive GFP/mCherry cells were quantified by flow cytometry to assess viral infection capacity. B,C) Following the strategy described in A, A549 cells expressing GFP-hACE2 were assayed for cell entry by SARS-CoV-2 pseudovirus expressing either empty vector (Δ Spike) or Spike protein corresponding to origin variant (Wuhan-1) or variants-of-concern Alpha, Beta, Delta or Zeta. A representative flow cytometry experiment is shown. Bars demonstrate mean and Standard Error of Mean while each data point represents a unique experiment; ****P < 0.0001; **P < 0.01; *P < 0.1 by t test.
Techniques Used: Generated, Transfection, Flow Cytometry, Infection, Expressing, Plasmid Preparation, Variant Assay
Figure Legend Snippet: A549 cells were transfected with either GFP-ACE2 WT, GFP-ACE2 polymorphisms or GFP alone. Then, ACE2 protein expression was analyzed by A) Western Blot with MA5-32307 antibody B) Immunocytochemistry (red) with either MA5-32307 antibody (left panel) or MAB933 antibody (right panel). Nuclei was stained with DAPI (blue). Transfected cells contain GFP (green). CT: secondary antibody control to detect unspecific binding. Images were acquired with Cell Observer-Zeiss. Scale bar: 50 μm
Techniques Used: Transfection, Expressing, Western Blot, Immunocytochemistry, Staining, Control, Binding Assay
Figure Legend Snippet: A) Studying the effect of ACE2 SNPs. Non-synonymous ACE2 single nucleotide polymorphism were selected among those fulfilling the triple criteria of high allelic frequency (Allele freq > 1.00e-4; Allele count > 20); involved in ACE2-claw S-protein RBD-binding interface and previously associated to clinical outcome. B, C) Following the strategy described in , A549 cells expressing either GFP-ACE2 either WT or polymorphisms were assayed for cell entry by SARS-CoV-2 pseudovirus expressing either empty vector (Δ Spike) or Spike protein corresponding to origin variant (Wuhan-1) or variants-of-concern Alpha, Beta, Delta or Zeta. A representative flow cytometry experiment is shown. Bars demonstrate mean and Standard Error of Mean while each data point represents a unique experiment; ***P < 0.001, **P < 0.01, *P < 0.1, ns P >0.1 to WT by t test.
Techniques Used: Binding Assay, Expressing, Plasmid Preparation, Variant Assay, Flow Cytometry
Figure Legend Snippet: A) Heatmap showing the distribution of ACE2 variants in the hospitalization severity groups. Coloured squares indicate de presence of the ACE2 variants. Red: Promoting; Green: Protective; Yellow: No-effect. B) Frequencies of ACE2 SNPs among hospitalization severity groups. Bars represent frequencies of the SNP in each group C) Frequencies of ACE2 SNPS among susceptibility groups.
Techniques Used:
Figure Legend Snippet:
Techniques Used:
Figure Legend Snippet:
Techniques Used: Infection
Figure Legend Snippet: GDF15 and ACE2 levels across ACE2 variants.
Techniques Used:
Figure Legend Snippet: GDF15 and ACE2 levels among ICU and non-ICU COVID-19 patients and ACE2 genotypes.
Techniques Used:
Figure Legend Snippet:
Techniques Used:
Figure Legend Snippet: Genotype 0 corresponding to subjects that did not carry any variant; Genotype 1, subjects that carry at least one promoting variant; Genotype 2, subjects that carry at least one protective variant; Genotype 3, subjects that carry at least one promoting and one protective variant ( ; ). We only found differences in the levels of the ACE2 mRNA ( P <0.05).
Techniques Used: Variant Assay
Figure Legend Snippet:
Techniques Used:
Figure Legend Snippet: Then, PCR1, PCR2 and PCR3 were performed in order to amplify the regions comprising the studied SNPs. PCR products were sequenced and aligned against reference ACE2 (NM_021804). PCR1: residues from Ser3 to Met249 (743 bp), PCR2: residues Phe308 to Arg621 (944 bp), PCR3: residues from Val670 to Val752 (250 bp) A) Agarose gel electrophoresis with the PCR products of several patients was performed to confirm specificity. B) Representative image of the alignment of the sequenced (forward and reverse) PCR products against reference ACE2 using SnapGene® Software. In particular, the image corresponds to the PCR2 of the non-ICU patient 10 that presents the L351V variant.
Techniques Used: Agarose Gel Electrophoresis, Software, Variant Assay
Related Articles
Enzyme-linked Immunosorbent Assay:Article Title: Antiviral Nanobiologic Therapy Remodulates Innate Immune Responses to Highly Pathogenic Coronavirus Article Snippet: .. The content of hACE2 of Article Title: Antiviral Nanobiologic Therapy Remodulates Innate Immune Responses to Highly Pathogenic Coronavirus. Article Snippet: .. The content of Article Title: Inhalation of ACE2 as a therapeutic target on sex-bias differences in SARS-CoV-2 infection and variant of concern Article Snippet: 17β estradiol ELISA kit , Abcam , Cat# AB108667. .. hACE2 ELISA kit , Abcam , Cat# Article Title: Inhalation of ACE2 as a therapeutic target on sex-bias differences in SARS-CoV-2 infection and variant of concern Article Snippet: The RAGE concentration was measured using an ELISA kit (Abcam, ab190807). .. Lung tissue homogenates were centrifuged for 15 min at 13,000 rpm 4°C, and the collected supernatants were used to measure the concentration of 17β estradiol (Abcam, ab108667), Article Title: Inhalation of ACE2 as a therapeutic target on sex-bias differences in SARS-CoV-2 infection and variant of concern Article Snippet: .. The hACE2 concentration in both rabbit and Concentration Assay:Article Title: Inhalation of ACE2 as a therapeutic target on sex-bias differences in SARS-CoV-2 infection and variant of concern Article Snippet: The RAGE concentration was measured using an ELISA kit (Abcam, ab190807). .. Lung tissue homogenates were centrifuged for 15 min at 13,000 rpm 4°C, and the collected supernatants were used to measure the concentration of 17β estradiol (Abcam, ab108667), Article Title: Inhalation of ACE2 as a therapeutic target on sex-bias differences in SARS-CoV-2 infection and variant of concern Article Snippet: .. The hACE2 concentration in both rabbit and Clinical Proteomics:Article Title: Inhalation of ACE2 as a therapeutic target on sex-bias differences in SARS-CoV-2 infection and variant of concern Article Snippet: .. The hACE2 concentration in both rabbit and |