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Abcam ab102370
Key resources
Ab102370, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ab102396/Goat+Anti-Horse+IgG+H%26L/pmc09353509-25-8-6
Average 99 stars, based on 63 article reviews
ab102370 - by Bioz Stars, 2026-09
99/100 stars

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1) Product Images from "Ca2+ imaging of self and other in medial prefrontal cortex during social dominance interactions in a tube test"

Article Title: Ca2+ imaging of self and other in medial prefrontal cortex during social dominance interactions in a tube test

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.2107942119

Key resources
Figure Legend Snippet: Key resources

Techniques Used: Software, Microscopy

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Incubation:

Article Title: Hydrogel-based 3D human iPSC-derived neuronal culture for the study of rabies virus infection
Article Snippet: After the blocking step, appropriate primary antibody dilutions of rabbit α-MAP2 (Abcam; ab183830), rabbit α-β-III tubulin (Abcam; ab18207), rat α-CTIP2 (Abcam; ab 18465), mouse α-PSD95 (Novus Biologicals; nb300-556) and horse α-rabies antiserum were added and incubated overnight at 4°C. .. The following day, gels were washed six times with PBS (5 mins per each round) and incubated with secondary antibodies, namely Alexa Fluor ® 568-conjugated goat α-rabbit (Invitrogen) and Dylight 488-conjugated goat α-horse IgG (Abcam; ab102391), at 1:250 dilutions. .. Alexa Fluor 488 Phalloidin (Thermo Fisher Scientific) was also used.

Article Title: Topical and systemic GLP-1R agonist administration both rescue retinal ganglion cells in hypertensive glaucoma
Article Snippet: Following serial washes with 0.5% Triton X-100 in PBS (TPBS) and 15-min permeabilization through freeze/thaw at −80°C, retinas were incubated overnight at 4°C with guinea pig anti-RBPMS (ABN1376, MilliporeSigma, Burlington, MA, USA) diluted 1:500 in a blocking buffer (2% bovine serum albumin and 2% Triton X-100 in PBS) and rabbit anti-Iba1 (ab178846, Abcam, Cambridge, UK) diluted 1:1000 in the same blocking buffer. .. The following day, retinas were washed in TPBS and incubated for 3 h at RT with Alexa Fluor 488 donkey anti-rabbit secondary antibody (A21206, Invitrogen, Waltham, MA, USA) and Cy3 goat anti-guinea pig secondary antibody (ab102370, Abcam), diluted 1:1000 and 1:500, respectively, in blocking buffer. .. Finally, retinas were washed, and cover slipped with Vectashield mounting medium plus DAPI for fluorescence (Vector Laboratories Inc., Burlingame, CA, USA).

Blocking Assay:

Article Title: Topical and systemic GLP-1R agonist administration both rescue retinal ganglion cells in hypertensive glaucoma
Article Snippet: Following serial washes with 0.5% Triton X-100 in PBS (TPBS) and 15-min permeabilization through freeze/thaw at −80°C, retinas were incubated overnight at 4°C with guinea pig anti-RBPMS (ABN1376, MilliporeSigma, Burlington, MA, USA) diluted 1:500 in a blocking buffer (2% bovine serum albumin and 2% Triton X-100 in PBS) and rabbit anti-Iba1 (ab178846, Abcam, Cambridge, UK) diluted 1:1000 in the same blocking buffer. .. The following day, retinas were washed in TPBS and incubated for 3 h at RT with Alexa Fluor 488 donkey anti-rabbit secondary antibody (A21206, Invitrogen, Waltham, MA, USA) and Cy3 goat anti-guinea pig secondary antibody (ab102370, Abcam), diluted 1:1000 and 1:500, respectively, in blocking buffer. .. Finally, retinas were washed, and cover slipped with Vectashield mounting medium plus DAPI for fluorescence (Vector Laboratories Inc., Burlingame, CA, USA).

Western Blot:

Article Title: Intracellular common gardens reveal niche differentiation in transposable element community during bacterial adaptive evolution
Article Snippet: .. Briefly, mouse monoclonal Anti-FLAG M2 antibody (Sigma), HRP (horseradish peroxidase) conjugated goat Anti-mouse IgG (Abcam), and eECL Western blot kit (CWBIO, Beijing, China) were used, and chemiluminescence signals were visualized using Fusion FX6 (Vilber) and Evolution-Capt Edge software. ..

Software:

Article Title: Intracellular common gardens reveal niche differentiation in transposable element community during bacterial adaptive evolution
Article Snippet: .. Briefly, mouse monoclonal Anti-FLAG M2 antibody (Sigma), HRP (horseradish peroxidase) conjugated goat Anti-mouse IgG (Abcam), and eECL Western blot kit (CWBIO, Beijing, China) were used, and chemiluminescence signals were visualized using Fusion FX6 (Vilber) and Evolution-Capt Edge software. ..

Staining:

Article Title: Hyperglycaemia, pregnancy outcomes and maternal metabolic disease risk during pregnancy and lactation in a lean gestational diabetes mouse model.
Article Snippet: .. Sections were stained with guinea pig anti-insulin primary antibody (7.4 mg/l, cat. no. A0564, Dako, Glostrup, Denmark), rabbit anti-guinea pig IgG H&L secondary antibody (5 mg/l, cat. no. Ab102360, Abcam, Cambridge, UK), and goat anti-rabbit immunoglobulins/horseradish peroxidase tertiary antibody (1.25 mg/l, cat. no. P0448, Dako, Glostrup, Denmark), followed by DAB peroxidase substrate kit (Substrate Kit-4100, Vector Laboratories Inc., Newark, CA, USA), and Mayer’s haematoxylin staining (cat. no. MHS32-1L, Sigma-Aldrich, Merck). .. Slides were scanned using a Hamamatsu NanoZoomer (Hamamatsu Photonics, Hamamatsu, Japan).



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Image Search Results


Key resources

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Ca2+ imaging of self and other in medial prefrontal cortex during social dominance interactions in a tube test

doi: 10.1073/pnas.2107942119

Figure Lengend Snippet: Key resources

Article Snippet: Goat Anti-Guinea pig IgG Cy3-conjugated , Abcam , ab102370.

Techniques: Software, Microscopy

Key resources

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Ca2+ imaging of self and other in medial prefrontal cortex during social dominance interactions in a tube test

doi: 10.1073/pnas.2107942119

Figure Lengend Snippet: Key resources

Article Snippet: Goat Anti-Guinea pig IgG Cy3-conjugated , Abcam , ab102370.

Techniques: Software, Microscopy