ars α1d  (Alomone Labs)

Journal: PLoS ONE

Article Title: Sympathetic Neurotransmitters Modulate Osteoclastogenesis and Osteoclast Activity in the Context of Collagen-Induced Arthritis

doi: 10.1371/journal.pone.0139726

Figure Lengend Snippet: Relative quantitative real-time PCR.

Article Snippet: Neurotransmitter receptors were detected using: rabbit antibody against rat PACAP receptor 1 (1:50, sc–30018, Santa Cruz Biotechnologies, Dallas, TX, USA); rabbit antibody against muscarinic ACh receptor M5 (1:400, ab41171) and rabbit antibody against adrenoceptor β2 (3μg/ml, ab36956; both Abcam, Cambridge, UK), as well as rabbit antibodies against extracellular sections of adrenoceptors α1D (1:100, #AAR–019) and α2B (1:100, #AAR–021; Alomone Labs, Jerusalem, Israel).

Techniques: Marker, Activity Assay

Journal: PLoS ONE

Article Title: Sympathetic Neurotransmitters Modulate Osteoclastogenesis and Osteoclast Activity in the Context of Collagen-Induced Arthritis

doi: 10.1371/journal.pone.0139726

Figure Lengend Snippet: Adrenergic receptors α1D, α2B, β2; muscarinic ACh receptor M5 and the alternative VIP receptor PACAP receptor 1 were stained positive on osteoclasts derived from BMMs of controls and CIA animals 20 (A) and 40 (B) days post-immunization. Paraformaldehyde-fixed cells were stained after 5 days differentiation. Nuclei were counterstained with DAPI. Cells containing 3 or more nuclei are considered to be osteoclasts (white circles). N = 4 rats. Magnification 200x. ACh: acetylcholine, AR: adrenoceptor, BMM: bone marrow-derived macrophages, CIA: collagen-induced arthritis, mAChR: muscarinic ACh receptor, NA: noradrenaline, PACAP: pituitary adenylate cyclase-activating peptide, VIP: vasoactive intestinal peptide

Article Snippet: Neurotransmitter receptors were detected using: rabbit antibody against rat PACAP receptor 1 (1:50, sc–30018, Santa Cruz Biotechnologies, Dallas, TX, USA); rabbit antibody against muscarinic ACh receptor M5 (1:400, ab41171) and rabbit antibody against adrenoceptor β2 (3μg/ml, ab36956; both Abcam, Cambridge, UK), as well as rabbit antibodies against extracellular sections of adrenoceptors α1D (1:100, #AAR–019) and α2B (1:100, #AAR–021; Alomone Labs, Jerusalem, Israel).

Techniques: Staining, Derivative Assay

Journal: Arthritis Research & Therapy

Article Title: Reactivity of rat bone marrow-derived macrophages to neurotransmitter stimulation in the context of collagen II-induced arthritis

doi: 10.1186/s13075-015-0684-4

Figure Lengend Snippet: Neurotransmitter receptor profile during course of CIA. Adrenergic receptors alpha 1D, alpha 2B, and beta 2 and muscarinic ACh receptor M5 and the alternative VIP receptor PACAP receptor 1 were stained in BMMs from control and CIA animals 20 ( a ) and 40 ( b ) days post immunization. Paraformaldehyde-fixed cells were stained after 5 days of differentiation in the presence of M-CSF and RANKL, and sections containing only BMMs were photographed. Nuclei were counterstained with DAPI. N = 4 (control and CIA). Magnification 200×. ACh acetylcholine, AChR acetylcholine receptor, BMM bone marrow-derived macrophage, CIA collagen II-induced arthritis, DAPI 4′,6-diamidino-2-phenylindole, M-CSF macrophage colony-stimulating factor, NA noradrenaline, PACAP pituitary adenylate cyclase-activating peptide, RANKL receptor activator of nuclear factor-kappa-B ligand, VIP vasoactive intestinal peptide

Article Snippet: Neurotransmitter receptors were detected by using rabbit polyclonal immunoglobuline (Ig) G against rat pituitary adenylate cyclase-activating peptide (PACAP) receptor 1 (1:50, sc-30018, Santa Cruz Biotechnologies, Dallas, TX, USA) and rabbit polyclonal antibody against muscarinic ACh receptor M5 (1:400, ab41171) and rabbit polyclonal antibody against adrenoceptor (AR) β2 (3 μg/ml, ab36956; both Abcam, Cambridge, UK) as well as rabbit polyclonal antibodies against extracellular sections of ARs α1D (1:100, #AAR-019) and α2B (1:100, #AAR-021; Alomone Labs, Jerusalem, Israel).

Techniques: Staining, Control, Derivative Assay