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isotype matched control rat 335 igg1  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec isotype matched control rat 335 igg1
    Isotype Matched Control Rat 335 Igg1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a-335/Isotype+Control+Antibody%2C+rat+IgG1/pm42392182-124-0-7
    Average 94 stars, based on 48 article reviews
    isotype matched control rat 335 igg1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Control:

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: GLAST (ACSA-1) Antibody, anti-human/mouse/rat, APC , Miltenyi Biotec, #130–123–555. .. Isotype Control Antibody, mouse IgG2a, APC , Miltenyi Biotec, #130–113–831. .. Animal model , Brain injection coordinates were experimentally tested on an animal cohort and adapted to animal size. Injection precision was visually tested during tissue dissection. Quality of fluid pumping by osmotic minipumps was verified after a priming period and prior to cannula implantation by visual inspection of fluid passed through the catheters. Volume of FC pumped into SN was checked by control of residual fluid in the pump after explantation. Each set of osmotic minipumps had been certified by Alzet pumping rate with standard deviation. Any outlier animals or tissue were excluded. Exclusion criteria for tissue collection were brain haemorrhage, abscess, and weight loss. Only verified tissues were pulled for further processing..

    Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation
    Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding isotype control antibody [REA control antibody IgG (Miltenyi, ref. no. 130–104-613)] was added and samples were incubated for 30 min at 37°C, 5% CO 2 in the dark. .. The cells were washed twice with staining buffer and analyzed with a SH800S cell sorter (Sony).

    Article Title: Increased Gene Therapy Efficacy Through the Use of Extended Half-Life Clotting Factors.
    Article Snippet: This tolerance 327 was previously found to be mediated by regulatory T cells (Tregs) in many studies.27-328 33 To determine if these cells are involved in the induction of immune tolerance in our 329 experiment, CD25+ Tregs were depleted using a protocol adapted based on Cao et 330 al.30 In particular, Tregs were selectively depleted by three successive injections with 331 200 μg (i.e. total 600 μg) of Ultra-LEAFTM purified rat anti-mouse CD25 (PC61, 332 BioLegend) over the course of two weeks into C56BL/6Jrj mice that were previously 333 injected with 11010 vg per mouse of AAV8 or AAVDJ/8 vectors expressing FIX-R338L 334 or FIX-R338L-ALB(QMP) (Supplemental Figure 3B). .. Isotype-matched control rat 335 IgG1 (clone ES26-14D1.11, Miltenyi BioTec) was used as control. ..

    Article Title: CRISPR/nCas9-Edited CD34+ Cells Rescue Mucopolysaccharidosis IVA Fibroblasts Phenotype.
    Article Snippet: .. Isotype Control Antibody, mouse IgG2a, APC (1:50, Cat. 130-113- 269, Miltenyi Biotec), REA Control Antibody (S), human IgG1, PE (1:50, Cat. 130-113-438, Miltenyi Biotec), and Isotype Control Antibody, mouse IgG2a, FITC (1:50, Cat. 130-113-279, Miltenyi Biotec) were used as isotype controls. ..

    Article Title: A developmentally regulated long-range enhancer-promoter contact mediates human neural development
    Article Snippet: .. Each sample was incubated with 2 μL of SSEA4 488 (Miltenyi), TRA-1-81 PE (Miltenyi) or an IgG1 isotype control antibody with matching fluorophore (Miltenyi) at 4 °C for 10 min. ..

    Article Title: In Vivo Endothelial Cell Gene Silencing by siRNA-LNPs Tuned with Lipoamino Bundle Chemical and Ligand Targeting.
    Article Snippet: Although small-interfering RNAs (siRNAs) are specific silencers for numerous disease-related genes, their clinical applications still require safe and effective means of delivery into target cells.. Highly efficient lipid nanoparticles (LNPs) are developed for siRNA delivery, showcasing the advantages of novel pH-responsive lipoamino xenopeptide (XP) carriers.. These sequence-defined XPs are assembled by branched lysine linkages between cationizable polar succinoyl tetraethylene pentamine (Stp) units and apolar lipoamino fatty acids (LAFs) at various ratios into bundle or U-shape topologies.

    Article Title: Anti-TSPAN8/anti-CD3 bispecific antibody and anti-TSPAN8 antibody
    Article Snippet: To each of the other four microtubes, 5 μL of CD326 (EpCAM)-PE (Miltenyi Biotec, 130-091-253) was added, 2.5 μL (0.25 g/tube) of 16B11, 16B12, 9F6 or 18C10 was added to each of the microtubes, and the cell was stained to obtain four evaluation antibody samples. .. In all the experiments, Isotype Control Antibody, mouse IgG1 (130-113-196, Miltenyi) was used as an isotype control. .. Further, for staining the cell, IgG2a-VioBlue antibody (Miltenyi Biotec, 130-113-277) and CD326(EpCAM)-VioBlue antibody (Miltenyi Biotec, 131-113-266) was used.

    Staining:

    Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation
    Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding isotype control antibody [REA control antibody IgG (Miltenyi, ref. no. 130–104-613)] was added and samples were incubated for 30 min at 37°C, 5% CO 2 in the dark. .. The cells were washed twice with staining buffer and analyzed with a SH800S cell sorter (Sony).

    Fluorescence:

    Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation
    Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding isotype control antibody [REA control antibody IgG (Miltenyi, ref. no. 130–104-613)] was added and samples were incubated for 30 min at 37°C, 5% CO 2 in the dark. .. The cells were washed twice with staining buffer and analyzed with a SH800S cell sorter (Sony).

    Labeling:

    Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation
    Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding isotype control antibody [REA control antibody IgG (Miltenyi, ref. no. 130–104-613)] was added and samples were incubated for 30 min at 37°C, 5% CO 2 in the dark. .. The cells were washed twice with staining buffer and analyzed with a SH800S cell sorter (Sony).

    Incubation:

    Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation
    Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding isotype control antibody [REA control antibody IgG (Miltenyi, ref. no. 130–104-613)] was added and samples were incubated for 30 min at 37°C, 5% CO 2 in the dark. .. The cells were washed twice with staining buffer and analyzed with a SH800S cell sorter (Sony).

    Article Title: A developmentally regulated long-range enhancer-promoter contact mediates human neural development
    Article Snippet: .. Each sample was incubated with 2 μL of SSEA4 488 (Miltenyi), TRA-1-81 PE (Miltenyi) or an IgG1 isotype control antibody with matching fluorophore (Miltenyi) at 4 °C for 10 min. ..



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