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il ab16667 ab209775 nb100 77417 sa00001 2 anti mouse igg hrp ib ib  (Proteintech)


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    Structured Review

    Proteintech il ab16667 ab209775 nb100 77417 sa00001 2 anti mouse igg hrp ib ib
    Il Ab16667 Ab209775 Nb100 77417 Sa00001 2 Anti Mouse Igg Hrp Ib Ib, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 8823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/NB100-77417/Goat+anti-rabbit+IgG+(H%2BL)%2C+HRP+conjugate/pmc11238352__13046_2024_3106_MOESM2_ESM-5-28-39
    Average 98 stars, based on 8823 article reviews
    il ab16667 ab209775 nb100 77417 sa00001 2 anti mouse igg hrp ib ib - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. The following day, membranes were washed five times with TBST and then incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit or anti-mouse IgG secondary antibodies (Proteintech, 1:10,000) for 1 h at room temperature. .. Immunoreactive bands were visualized using an ECL chemiluminescent substrate (Thermo Scientific, USA) and detected with using the Tanon system (TANON-5200CE).

    Article Title: ACOD-itaconate in macrophage attenuates oxidative stress and inflammation in benign airway stenosis by upregulating and transferring FTH1
    Article Snippet: Equal amounts of protein and a protein ladder (Epizyme #WJ103) were loaded onto the gel and electrophoresed at 80-120 V for 60 min. Proteins were then transferred onto a 0.45 μm PVDF membrane using a methanol-based transfer buffer. .. After blocking, the membrane was incubated overnight at 4 °C with primary antibodies, followed by incubation with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:8000, Proteintech #SA00001-2). .. Protein bands were visualized using the Tanon 5200 chemiluminescent imaging system (Shanghai) and quantified with ImageJ software.

    Article Title: Hypoxia-induced exosomal CAMTA1 promotes radio-resistance in MDA-MB-231 cells by regulating NRG1 to mediate M2 macrophage polarization
    Article Snippet: Blocked by 5% BSA (MilliporeSigma) for 1 h at room temperature, the membranes were incubated with primary antibodies against NRG1 (cat. no. 10527-1-AP; 1:1,000; Proteintech Group, Inc.), hypoxia-inducible factor-1α (HIF-1α; cat. no. 20960-1-AP; 1:2,000; Proteintech Group, Inc.), CD63 (cat. no. 25682-1-AP; 1:2,000; Proteintech Group, Inc.), CD81 (cat. no. 27855-1-AP; 1:1,000; Proteintech Group, Inc.), CD9 (cat. no. 20597-1-AP; 1:2,000; Proteintech Group, Inc.), ALIX (cat. no. 12422-1-AP; 1:5,000; Proteintech Group, Inc.) and GAPDH (cat. no. 10494-1-AP; 1:5,000; Proteintech Group, Inc.) overnight at 4°C. .. On the next day, the membranes were incubated with goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies (cat. no. SA00001-2; Proteintech Group, Inc.) at room temperature for 3 h. The protein bands were visualized using ECL regent (Thermo Fisher Scientific, Inc.) and protein density was semi-quantified via ImageJ software (version 1.53; National Institutes of Health, USA). ..

    Article Title: Multi-omics analysis reveals RBPJ-mediated regulation of EGF/ACTN2 / MYPN / COL21A1 in fibroblast during oviduct functional remodeling of duck
    Article Snippet: .. Subsequently, the membrane was incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:10000 dilution, SA00001-2, Proteintech, China) or HRP-conjugated goat anti-mouse IgG (1:10000 dilution, SA00001-1, Proteintech, China) at RT for 1 h, depending on the host species of the primary antibody. ..

    Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
    Article Snippet: .. After washing, the membranes were incubated with secondary antibodies (HRP-conjugated goat anti-rabbit IgG) at room temperature for 2 h (1:10,000; cat. no. SA00001-2; Proteintech Group, Inc.). .. Protein detection was performed using an ultra-sensitive enhanced chemiluminescence kit (Shanghai Yamei Biomedical Technology Co Ltd.) and using ImageJ software (version 1.53k; National Institutes of Health).

    Blocking Assay:

    Article Title: ACOD-itaconate in macrophage attenuates oxidative stress and inflammation in benign airway stenosis by upregulating and transferring FTH1
    Article Snippet: Equal amounts of protein and a protein ladder (Epizyme #WJ103) were loaded onto the gel and electrophoresed at 80-120 V for 60 min. Proteins were then transferred onto a 0.45 μm PVDF membrane using a methanol-based transfer buffer. .. After blocking, the membrane was incubated overnight at 4 °C with primary antibodies, followed by incubation with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:8000, Proteintech #SA00001-2). .. Protein bands were visualized using the Tanon 5200 chemiluminescent imaging system (Shanghai) and quantified with ImageJ software.

    Membrane:

    Article Title: ACOD-itaconate in macrophage attenuates oxidative stress and inflammation in benign airway stenosis by upregulating and transferring FTH1
    Article Snippet: Equal amounts of protein and a protein ladder (Epizyme #WJ103) were loaded onto the gel and electrophoresed at 80-120 V for 60 min. Proteins were then transferred onto a 0.45 μm PVDF membrane using a methanol-based transfer buffer. .. After blocking, the membrane was incubated overnight at 4 °C with primary antibodies, followed by incubation with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:8000, Proteintech #SA00001-2). .. Protein bands were visualized using the Tanon 5200 chemiluminescent imaging system (Shanghai) and quantified with ImageJ software.

    Article Title: Multi-omics analysis reveals RBPJ-mediated regulation of EGF/ACTN2 / MYPN / COL21A1 in fibroblast during oviduct functional remodeling of duck
    Article Snippet: .. Subsequently, the membrane was incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:10000 dilution, SA00001-2, Proteintech, China) or HRP-conjugated goat anti-mouse IgG (1:10000 dilution, SA00001-1, Proteintech, China) at RT for 1 h, depending on the host species of the primary antibody. ..

    other:

    Article Title: Ultrasound-assisted extraction optimization of Fructus Tribuli polysaccharides: How stir-frying processing alters structures and enhances antihypertensive efficacy
    Article Snippet: TLR4 polyclonal antibody (19811–1-AP), GAPDH (10494–1-AP) antibody, goat anti-mouse immunoglobulin (Ig)G (H + L) (SA00001-1), and goat anti-rabbit IgG (H + L) (SA00001-2) were purchased from Proteintech Group (Wuhan, China).



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