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biotinylated capture sars cov 2 n protein antibody  (EastCoast Bio)


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    EastCoast Bio biotinylated capture sars cov 2 n protein antibody
    Biotinylated Capture Sars Cov 2 N Protein Antibody, supplied by EastCoast Bio, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HM1025/Coronavirus+(COVID-19+%26+SARS-CoV+NP)+Antibody/pmc09186413-55-22-29
    Average 93 stars, based on 5 article reviews
    biotinylated capture sars cov 2 n protein antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Labeling:

    Article Title: Optofluidic multiplex detection of single SARS-CoV-2 and influenza A antigens using a novel bright fluorescent probe assay
    Article Snippet: .. Capture antibodies are anti-SARS-CoV-2 N protein antibodies (HM1054, East Coast Bio) and anti-influenza A antigen (HM418, East Coast Bio) were ordered and labeled with biotin in house. .. An aliquot of antibody is added to a 20× molar excess of EZ-Link Sulfo-NHS-LC-LC-Biotin (Thermo Fisher Scientific) according to manufacturer’s instructions.

    Article Title: Ultrasensitive detection of SARS-CoV-2 RNA and antigen using single-molecule optofluidic chip
    Article Snippet: .. 2 μ g (≈5× molar excess) of a secondary, DBCO labeled anti-SARS-CoV-2 N protein antibody (HM1055, East Coast Bio.) and 1 μ g of target (SARS-CoV-2 N protein model, East Coast Bio.) antigens spiked in 50 μ l of NP swab were added to the bead-pulldown complex and incubated at 37 °C for 2 h. Importantly, in order to create a probe that is bright enough for single protein detection, we developed a novel reporter based on modified template DNA with biotinylated-dUPT, tagged with multiple cyanine dyes. .. Briefly, 1 kbp of pUC-19 PCR product was made with photocleavable azide forward primer (IDT) and 50% biotinylated-dUTP.

    Incubation:

    Article Title: Ultrasensitive detection of SARS-CoV-2 RNA and antigen using single-molecule optofluidic chip
    Article Snippet: .. 2 μ g (≈5× molar excess) of a secondary, DBCO labeled anti-SARS-CoV-2 N protein antibody (HM1055, East Coast Bio.) and 1 μ g of target (SARS-CoV-2 N protein model, East Coast Bio.) antigens spiked in 50 μ l of NP swab were added to the bead-pulldown complex and incubated at 37 °C for 2 h. Importantly, in order to create a probe that is bright enough for single protein detection, we developed a novel reporter based on modified template DNA with biotinylated-dUPT, tagged with multiple cyanine dyes. .. Briefly, 1 kbp of pUC-19 PCR product was made with photocleavable azide forward primer (IDT) and 50% biotinylated-dUTP.

    Article Title: Ultrasensitive detection of SARS-CoV-2 RNA and antigen using single-molecule optofluidic chip
    Article Snippet: .. Specifically, 5 μ l of magnetic beads (9 × 10 9 beads/ml) were incubated with 5 μ g (≈5× molar excess) of biotinylated capture SARS-CoV-2 N protein antibody (HM1054, East Coast Bio). ..

    Modification:

    Article Title: Ultrasensitive detection of SARS-CoV-2 RNA and antigen using single-molecule optofluidic chip
    Article Snippet: .. 2 μ g (≈5× molar excess) of a secondary, DBCO labeled anti-SARS-CoV-2 N protein antibody (HM1055, East Coast Bio.) and 1 μ g of target (SARS-CoV-2 N protein model, East Coast Bio.) antigens spiked in 50 μ l of NP swab were added to the bead-pulldown complex and incubated at 37 °C for 2 h. Importantly, in order to create a probe that is bright enough for single protein detection, we developed a novel reporter based on modified template DNA with biotinylated-dUPT, tagged with multiple cyanine dyes. .. Briefly, 1 kbp of pUC-19 PCR product was made with photocleavable azide forward primer (IDT) and 50% biotinylated-dUTP.

    Magnetic Beads:

    Article Title: Ultrasensitive detection of SARS-CoV-2 RNA and antigen using single-molecule optofluidic chip
    Article Snippet: .. Specifically, 5 μ l of magnetic beads (9 × 10 9 beads/ml) were incubated with 5 μ g (≈5× molar excess) of biotinylated capture SARS-CoV-2 N protein antibody (HM1054, East Coast Bio). ..



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