Review



mouse monoclonal anti cd34  (Hycult Biotech)


Bioz Verified Symbol Hycult Biotech is a verified supplier
Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Hycult Biotech mouse monoclonal anti cd34
    Treatment of xenografted nude mice was started thirteen days after s.c. PC3 cell injection. Experimental group (n = 8) was treated six times per week p.t. with 100 µL Drs B2 (2.5 mg/kg body weight). Control group (n = 7) was treated six times per week p.t. with 100 µL of PBS. A ) The effect of Drs B2 on tumor size versus time of treatment. After 47 days of treatment the mice were sacrificed, body weight was observed; tumors were isolated, B ) weighted and stored at −80°C. C ) PC3 tumor proliferation was evaluated by Ki67 staining of frozen tissue sections. Proliferation was quantified by image J software analysis of Ki67 positive stained cells on the whole tumor section. The data are mean areas ± SD obtained from 6 control mice and 4 mice treated with Drs B2. Scale bar, 50 µm. * p <0.05 versus control (PBS). D ) Tumor vessel formation was observed with anti <t>CD34</t> antibodies. Angiogenesis was quantified by image analysis of <t>CD34</t> <t>positive</t> stained endothelial cells. The data are mean areas ± SD obtained from 6 control mice and 4 mice treated with Drs B2. Scale bar, 50 µm * p <0.05 versus control (PBS).
    Mouse Monoclonal Anti Cd34, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HM1025/CD34%2C+Mouse%2C+mAb+MEC14%2E7/pmc03447859-193-0-5
    Average 93 stars, based on 36 article reviews
    mouse monoclonal anti cd34 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2"

    Article Title: Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0044351

    Treatment of xenografted nude mice was started thirteen days after s.c. PC3 cell injection. Experimental group (n = 8) was treated six times per week p.t. with 100 µL Drs B2 (2.5 mg/kg body weight). Control group (n = 7) was treated six times per week p.t. with 100 µL of PBS. A ) The effect of Drs B2 on tumor size versus time of treatment. After 47 days of treatment the mice were sacrificed, body weight was observed; tumors were isolated, B ) weighted and stored at −80°C. C ) PC3 tumor proliferation was evaluated by Ki67 staining of frozen tissue sections. Proliferation was quantified by image J software analysis of Ki67 positive stained cells on the whole tumor section. The data are mean areas ± SD obtained from 6 control mice and 4 mice treated with Drs B2. Scale bar, 50 µm. * p <0.05 versus control (PBS). D ) Tumor vessel formation was observed with anti CD34 antibodies. Angiogenesis was quantified by image analysis of CD34 positive stained endothelial cells. The data are mean areas ± SD obtained from 6 control mice and 4 mice treated with Drs B2. Scale bar, 50 µm * p <0.05 versus control (PBS).
    Figure Legend Snippet: Treatment of xenografted nude mice was started thirteen days after s.c. PC3 cell injection. Experimental group (n = 8) was treated six times per week p.t. with 100 µL Drs B2 (2.5 mg/kg body weight). Control group (n = 7) was treated six times per week p.t. with 100 µL of PBS. A ) The effect of Drs B2 on tumor size versus time of treatment. After 47 days of treatment the mice were sacrificed, body weight was observed; tumors were isolated, B ) weighted and stored at −80°C. C ) PC3 tumor proliferation was evaluated by Ki67 staining of frozen tissue sections. Proliferation was quantified by image J software analysis of Ki67 positive stained cells on the whole tumor section. The data are mean areas ± SD obtained from 6 control mice and 4 mice treated with Drs B2. Scale bar, 50 µm. * p <0.05 versus control (PBS). D ) Tumor vessel formation was observed with anti CD34 antibodies. Angiogenesis was quantified by image analysis of CD34 positive stained endothelial cells. The data are mean areas ± SD obtained from 6 control mice and 4 mice treated with Drs B2. Scale bar, 50 µm * p <0.05 versus control (PBS).

    Techniques Used: Injection, Isolation, Staining, Software

    Related Articles

    other:

    Article Title: DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells
    Article Snippet: Polyclonal rabbit anti-MCM6 (Proteintech, 13347-2-AP, 1:8000) and polyclonal rabbit anti-TIF1B/KAP1 (Merck Millipore, Sigma-Aldrich, ABE1859, 1:2000), mouse monoclonal anti-ICBP90/UHRF1 (Sigma-Aldrich, MABE308, 1:5000), rat monoclonal anti-HA-Peroxidase high affinity (Roche, Sigma-Aldrich, 12013819001, 1/1000), rabbit polyclonal anti-Actin (Sigma-Aldrich, A2103, 1/3000), mouse monoclonal anti-FLAG® M2-Peroxidase (Sigma-Aldrich, A8592, 1/5000), mouse monoclonal anti-Tropomyosin Ab (Sigma-Aldrich, T2780, 1/50), goat polyclonal anti-rabbit IgG–Peroxidase antibody (Sigma-Aldrich, A6154, 1/1000), Alexa Fluor® 488 Goat Anti-Mouse IgG antibody (Molecular Probes, Invitrogen, A-11001, A11029 , 1/100), Alexa Fluor® 594 Goat Anti-Rabbit IgG antibody (Molecular Probes, Invitrogen, A-11012, 1/100), FluoroshieldTM with DAPI (Sigma-Aldrich, F6057, 1/3), DynabeadsTM CD25 (Invitrogen, Thermo Fisher Scientific, 11157D), rat monoclonal anti-CD34 antibody (HycultBiotech, HM1015, 1/20), Polink-2 Plus HRP Rat-NM Bulk kit for DAB (GBI Labs, D46-110), rabbit monoclonal anti-Ki-67 (Neomarkers; LabVision, Thermo Fisher Scientific, RM-9106, 1/200), rabbit polyclonal cleaved Caspase-3 (Asp175) (Cell Signaling, 9661, 1/400), anti-FLAG M2 affinity gel (Sigma-Aldrich, A 2220), FLAG® Peptide (Sigma-Aldrich, F3290), Protein A agarose (Pierce, Thermo Fisher Scientific, 20333).

    Article Title: DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells.
    Article Snippet: Polyclonal rabbit anti-MCM6 (Proteintech, 13347-2-AP, 1:8000) and polyclonal rabbit anti-TIF1B/KAP1 (Merck Millipore, Sigma-Aldrich, ABE1859, 1:2000), mouse monoclonal anti-ICBP90/UHRF1 (SigmaAldrich, MABE308, 1:5000), rat monoclonal anti-HA-Peroxidase high affinity (Roche, Sigma-Aldrich, 12013819001, 1/1000), rabbit polyclonal anti-Actin (Sigma-Aldrich, A2103, 1/3000), mouse monoclonal anti-FLAG M2-Peroxidase (Sigma-Aldrich, A8592, 1/5000), mouse monoclonal anti-Tropomyosin Ab (Sigma-Aldrich, T2780, 1/50), goat polyclonal anti-rabbit IgG–Peroxidase antibody (Sigma-Aldrich, A6154, 1/1000), Alexa Fluor 488 Goat Anti-Mouse IgG antibody (Molecular Probes, Invitrogen, A-11001, A11029, 1/100), Alexa Fluor 594 Goat Anti-Rabbit IgG antibody (Molecular Probes, Invitrogen, A11012, 1/100), Fluoroshield with DAPI (Sigma-Aldrich, F6057, 1/3), Dynabeads CD25 (Invitrogen, Thermo Fisher Scientific, 11157D), rat monoclonal anti-CD34 antibody (HycultBiotech, HM1015, 1/20), Polink-2 Plus HRP Rat-NM Bulk kit for DAB (GBI Labs, D46-110), rabbit monoclonal anti-Ki-67 (Neomarkers; LabVision, Thermo Fisher Scientific, RM-9106, 1/200), rabbit polyclonal cleaved Caspase-3 (Asp175) (Cell Signaling, 9661, 1/400), anti-FLAG M2 affinity gel (Sigma-Aldrich, A 2220), FLAG Peptide (Sigma-Aldrich, F3290), Protein A agarose (Pierce, Thermo Fisher Scientific, 20333).

    Staining:

    Article Title: BR55 Ultrasound Molecular Imaging of Clear Cell Renal Cell Carcinoma Reflects Tumor Vascular Expression of VEGFR-2 in a Patient-Derived Xenograft Model.
    Article Snippet: Tumors were then fixed in a 4% paraformaldehyde solution diluted in PBS, embedded in paraffin and cut to a thickness of 4 μm with a microtome (Leica RM2245, Leica Biosystems, Wetzlar, Germany). .. Staining was performed by the PETRA platform (Experimental and Translational Pathology, Gustave Roussy Cancer Campus, Villejuif, France): standard HES (Hematoxylin Eosin Safran) staining to visualize the tissue integrity and morphology, immunolabeling with rat anti-mouse CD34 antibody (1:20, HM1015, clone MEC14.7, Hycult Biotech, Uden, The Netherlands) and rabbit monoclonal antihuman VEGFR-2 antibody (1:100, #2479, clone 55B11, Cell Signaling Technology, Danvers, MA, USA), and detected by the Bond Polymer refined detection kit (Leïca Biosystems, #DS9390). .. IHCstained slides were scanned in their entirety at 20× magnification (VS120, Olympus Life Science Solutions, Tokyo, Japan), as shown in Figure 8.

    Article Title: BR55 Ultrasound Molecular Imaging of Clear Cell Renal Cell Carcinoma Reflects Tumor Vascular Expression of VEGFR-2 in a Patient-Derived Xenograft Model.
    Article Snippet: Tumors were then fixed in a 4% paraformaldehyde solution diluted in PBS, embedded in paraffin and cut to a thickness of 4 μm with a microtome (Leica RM2245, Leica Biosystems, Wetzlar, Germany). .. Staining was performed by the PETRA platform (Experimental and Translational Pathology, Gustave Roussy Cancer Campus, Villejuif, France): standard HES (Hematoxylin Eosin Safran) staining to visualize the tis- sue integrity and morphology, immunolabeling with rat anti-mouse CD34 antibody (1:20, HM1015, clone MEC14.7, Hycult Biotech, Uden, The Netherlands) and rabbit monoclonal antihuman VEGFR-2 antibody (1:100, #2479, clone 55B11, Cell Signaling Technology, Dan- vers, USA, Massachusetts), and detected by the Bond Polymer refined detection kit (Leïca Biosystems, #DS9390). .. IHCstained slides were scanned in their entirety at 20× magnifica- tion (VS120, Olympus Life Science Solutions, Tokyo, Japan), as shown in Figure 8.

    Article Title: BR55 Ultrasound Molecular Imaging of Clear Cell Renal Cell Carcinoma Reflects Tumor Vascular Expression of VEGFR-2 in a Patient-Derived Xenograft Model
    Article Snippet: Tumors were then fixed in a 4% paraformaldehyde solution diluted in PBS, embedded in paraffin and cut to a thickness of 4 μm with a microtome (Leica RM2245, Leica Biosystems, Wetzlar, Germany). .. Staining was performed by the PETRA platform (Experimental and Translational Pathology, Gustave Roussy Cancer Campus, Villejuif, France): standard HES (Hematoxylin Eosin Safran) staining to visualize the tissue integrity and morphology, immunolabeling with rat anti-mouse CD34 antibody (1:20, HM1015, clone MEC14.7, Hycult Biotech, Uden, The Netherlands) and rabbit monoclonal antihuman VEGFR-2 antibody (1:100, #2479, clone 55B11, Cell Signaling Technology, Danvers, MA, USA), and detected by the Bond Polymer refined detection kit (Leïca Biosystems, #DS9390). .. IHCstained slides were scanned in their entirety at 20× magnification (VS120, Olympus Life Science Solutions, Tokyo, Japan), as shown in .

    Immunolabeling:

    Article Title: BR55 Ultrasound Molecular Imaging of Clear Cell Renal Cell Carcinoma Reflects Tumor Vascular Expression of VEGFR-2 in a Patient-Derived Xenograft Model.
    Article Snippet: Tumors were then fixed in a 4% paraformaldehyde solution diluted in PBS, embedded in paraffin and cut to a thickness of 4 μm with a microtome (Leica RM2245, Leica Biosystems, Wetzlar, Germany). .. Staining was performed by the PETRA platform (Experimental and Translational Pathology, Gustave Roussy Cancer Campus, Villejuif, France): standard HES (Hematoxylin Eosin Safran) staining to visualize the tissue integrity and morphology, immunolabeling with rat anti-mouse CD34 antibody (1:20, HM1015, clone MEC14.7, Hycult Biotech, Uden, The Netherlands) and rabbit monoclonal antihuman VEGFR-2 antibody (1:100, #2479, clone 55B11, Cell Signaling Technology, Danvers, MA, USA), and detected by the Bond Polymer refined detection kit (Leïca Biosystems, #DS9390). .. IHCstained slides were scanned in their entirety at 20× magnification (VS120, Olympus Life Science Solutions, Tokyo, Japan), as shown in Figure 8.

    Article Title: BR55 Ultrasound Molecular Imaging of Clear Cell Renal Cell Carcinoma Reflects Tumor Vascular Expression of VEGFR-2 in a Patient-Derived Xenograft Model.
    Article Snippet: Tumors were then fixed in a 4% paraformaldehyde solution diluted in PBS, embedded in paraffin and cut to a thickness of 4 μm with a microtome (Leica RM2245, Leica Biosystems, Wetzlar, Germany). .. Staining was performed by the PETRA platform (Experimental and Translational Pathology, Gustave Roussy Cancer Campus, Villejuif, France): standard HES (Hematoxylin Eosin Safran) staining to visualize the tis- sue integrity and morphology, immunolabeling with rat anti-mouse CD34 antibody (1:20, HM1015, clone MEC14.7, Hycult Biotech, Uden, The Netherlands) and rabbit monoclonal antihuman VEGFR-2 antibody (1:100, #2479, clone 55B11, Cell Signaling Technology, Dan- vers, USA, Massachusetts), and detected by the Bond Polymer refined detection kit (Leïca Biosystems, #DS9390). .. IHCstained slides were scanned in their entirety at 20× magnifica- tion (VS120, Olympus Life Science Solutions, Tokyo, Japan), as shown in Figure 8.

    Article Title: BR55 Ultrasound Molecular Imaging of Clear Cell Renal Cell Carcinoma Reflects Tumor Vascular Expression of VEGFR-2 in a Patient-Derived Xenograft Model
    Article Snippet: Tumors were then fixed in a 4% paraformaldehyde solution diluted in PBS, embedded in paraffin and cut to a thickness of 4 μm with a microtome (Leica RM2245, Leica Biosystems, Wetzlar, Germany). .. Staining was performed by the PETRA platform (Experimental and Translational Pathology, Gustave Roussy Cancer Campus, Villejuif, France): standard HES (Hematoxylin Eosin Safran) staining to visualize the tissue integrity and morphology, immunolabeling with rat anti-mouse CD34 antibody (1:20, HM1015, clone MEC14.7, Hycult Biotech, Uden, The Netherlands) and rabbit monoclonal antihuman VEGFR-2 antibody (1:100, #2479, clone 55B11, Cell Signaling Technology, Danvers, MA, USA), and detected by the Bond Polymer refined detection kit (Leïca Biosystems, #DS9390). .. IHCstained slides were scanned in their entirety at 20× magnification (VS120, Olympus Life Science Solutions, Tokyo, Japan), as shown in .

    Polymer:

    Article Title: BR55 Ultrasound Molecular Imaging of Clear Cell Renal Cell Carcinoma Reflects Tumor Vascular Expression of VEGFR-2 in a Patient-Derived Xenograft Model.
    Article Snippet: Tumors were then fixed in a 4% paraformaldehyde solution diluted in PBS, embedded in paraffin and cut to a thickness of 4 μm with a microtome (Leica RM2245, Leica Biosystems, Wetzlar, Germany). .. Staining was performed by the PETRA platform (Experimental and Translational Pathology, Gustave Roussy Cancer Campus, Villejuif, France): standard HES (Hematoxylin Eosin Safran) staining to visualize the tissue integrity and morphology, immunolabeling with rat anti-mouse CD34 antibody (1:20, HM1015, clone MEC14.7, Hycult Biotech, Uden, The Netherlands) and rabbit monoclonal antihuman VEGFR-2 antibody (1:100, #2479, clone 55B11, Cell Signaling Technology, Danvers, MA, USA), and detected by the Bond Polymer refined detection kit (Leïca Biosystems, #DS9390). .. IHCstained slides were scanned in their entirety at 20× magnification (VS120, Olympus Life Science Solutions, Tokyo, Japan), as shown in Figure 8.

    Article Title: BR55 Ultrasound Molecular Imaging of Clear Cell Renal Cell Carcinoma Reflects Tumor Vascular Expression of VEGFR-2 in a Patient-Derived Xenograft Model.
    Article Snippet: Tumors were then fixed in a 4% paraformaldehyde solution diluted in PBS, embedded in paraffin and cut to a thickness of 4 μm with a microtome (Leica RM2245, Leica Biosystems, Wetzlar, Germany). .. Staining was performed by the PETRA platform (Experimental and Translational Pathology, Gustave Roussy Cancer Campus, Villejuif, France): standard HES (Hematoxylin Eosin Safran) staining to visualize the tis- sue integrity and morphology, immunolabeling with rat anti-mouse CD34 antibody (1:20, HM1015, clone MEC14.7, Hycult Biotech, Uden, The Netherlands) and rabbit monoclonal antihuman VEGFR-2 antibody (1:100, #2479, clone 55B11, Cell Signaling Technology, Dan- vers, USA, Massachusetts), and detected by the Bond Polymer refined detection kit (Leïca Biosystems, #DS9390). .. IHCstained slides were scanned in their entirety at 20× magnifica- tion (VS120, Olympus Life Science Solutions, Tokyo, Japan), as shown in Figure 8.

    Article Title: BR55 Ultrasound Molecular Imaging of Clear Cell Renal Cell Carcinoma Reflects Tumor Vascular Expression of VEGFR-2 in a Patient-Derived Xenograft Model
    Article Snippet: Tumors were then fixed in a 4% paraformaldehyde solution diluted in PBS, embedded in paraffin and cut to a thickness of 4 μm with a microtome (Leica RM2245, Leica Biosystems, Wetzlar, Germany). .. Staining was performed by the PETRA platform (Experimental and Translational Pathology, Gustave Roussy Cancer Campus, Villejuif, France): standard HES (Hematoxylin Eosin Safran) staining to visualize the tissue integrity and morphology, immunolabeling with rat anti-mouse CD34 antibody (1:20, HM1015, clone MEC14.7, Hycult Biotech, Uden, The Netherlands) and rabbit monoclonal antihuman VEGFR-2 antibody (1:100, #2479, clone 55B11, Cell Signaling Technology, Danvers, MA, USA), and detected by the Bond Polymer refined detection kit (Leïca Biosystems, #DS9390). .. IHCstained slides were scanned in their entirety at 20× magnification (VS120, Olympus Life Science Solutions, Tokyo, Japan), as shown in .



    Similar Products

    Image Search Results