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anti c3d  (R&D Systems)


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    Structured Review

    R&D Systems anti c3d
    Anti C3d, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HM1025/Mouse+S100A10+Antibody/pm38740862-449-16-18
    Average 93 stars, based on 38 article reviews
    anti c3d - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: The Calcium‐Binding Protein S100A10 (p11) Is Required for Normal Motor Performance by Regulating Vesicle Dynamics at Excitatory Synapses
    Article Snippet: .. After PBS washing, sections were immersed in a blocking solution [2.5% (w/v) bovine serum albumin and 0.1% (v/v) Triton X‐100 in PBS] for 30 min, followed by incubation (4°C, overnight) with the polyclonal primary antibodies against GFAP (1:250; Santa Cruz Biotechnology, Cat# sc‐58766, RRID:AB_783554) developed in mouse, p11 (1:100, R&D systems, Cat# AF2377, RRID:AB_2183469) developed in goat, vGLUT2 (1:2000, Millipore Bioscience Research Reagents, Cat# AB2251, RRID:AB_1587626) developed in guinea pig, Munc13‐1 (1:200; Synaptic systems, Goettingen, Germany, Cat# 126‐102, RRID:AB_887734), and Synaptophysin (Syn) (1:200; Zymed Laboratories, San Francisco, CA, Cat# 18‐0130) developed in rabbit. .. Next, the tissue was rinsed with PBS and incubated for 1 h at room temperature with Cy2‐conjugated, Cy3‐conjugated, and/or Cy5‐conjugated anti‐goat, anti‐mouse, anti‐guinea pig, and/or anti‐rabbit IgGs (1:200; Jackson ImmunoResearch Laboratories) as secondary antibodies.

    Article Title: The Calcium-Binding Protein S100A10 (p11) Is Required for Normal Motor Performance by Regulating Vesicle Dynamics at Excitatory Synapses.
    Article Snippet: .. After PBS washing, sections were immersed in a blocking solution [2.5% (w/v) bovine serum albumin and 0.1% (v/v) Triton X- 100 in PBS] for 30 min, followed by incubation (4°C, overnight) with the polyclonal primary antibodies against GFAP (1:250; Santa Cruz Biotechnology, Cat# sc58766, RRID:AB_783554) developed in mouse, p11 (1:100, R&D systems, Cat# AF2377, RRID:AB_2183469) developed in goat, vGLUT2 (1:2000, Millipore Bioscience Research Reagents, Cat# AB2251, RRID:AB_1587626) developed in guinea pig, Munc13- 1 (1:200; Synaptic systems, Goettingen, Germany, Cat# 126- 102, RRID:AB_887734), and Synaptophysin (Syn) (1:200; Zymed Laboratories, San Francisco, CA, Cat# 18- 0130) developed in rabbit. .. Next, the tissue was rinsed with PBS and incubated for 1 h at room temperature with Cy2- conjugated, Cy3- conjugated, and/or Cy5- conjugated anti- goat, anti- mouse, anti- guinea pig, and/or anti- rabbit IgGs (1:200; Jackson ImmunoResearch Laboratories) as secondary antibodies.

    Incubation:

    Article Title: The Calcium‐Binding Protein S100A10 (p11) Is Required for Normal Motor Performance by Regulating Vesicle Dynamics at Excitatory Synapses
    Article Snippet: .. After PBS washing, sections were immersed in a blocking solution [2.5% (w/v) bovine serum albumin and 0.1% (v/v) Triton X‐100 in PBS] for 30 min, followed by incubation (4°C, overnight) with the polyclonal primary antibodies against GFAP (1:250; Santa Cruz Biotechnology, Cat# sc‐58766, RRID:AB_783554) developed in mouse, p11 (1:100, R&D systems, Cat# AF2377, RRID:AB_2183469) developed in goat, vGLUT2 (1:2000, Millipore Bioscience Research Reagents, Cat# AB2251, RRID:AB_1587626) developed in guinea pig, Munc13‐1 (1:200; Synaptic systems, Goettingen, Germany, Cat# 126‐102, RRID:AB_887734), and Synaptophysin (Syn) (1:200; Zymed Laboratories, San Francisco, CA, Cat# 18‐0130) developed in rabbit. .. Next, the tissue was rinsed with PBS and incubated for 1 h at room temperature with Cy2‐conjugated, Cy3‐conjugated, and/or Cy5‐conjugated anti‐goat, anti‐mouse, anti‐guinea pig, and/or anti‐rabbit IgGs (1:200; Jackson ImmunoResearch Laboratories) as secondary antibodies.

    Article Title: Bioadaptive liquid-infused multifunctional fibers for long-term neural recording via BDNF stabilization and enhanced neural interaction
    Article Snippet: For blocking of nonspecific binding and permeabilization, the sections were incubated in 0.3% Triton X-100 (Sigma-Aldrich) and 5% normal donkey serum (Abcam) solution for 1 hour. .. The sections were then incubated overnight at room temperature with primary antibodies: rabbit anti–MMP-9 (1:100; ab228402, Abcam), goat Iba1 (1:500; ab5076, Abcam), goat anti-S100A10 (10 μg/ml; AF2377, R&D Systems), rat anti-C3 (1:500; NB200-540, Novus Biologicals), rabbit anti-GFAP (1:250; ab68428, Abcam), and rat anti-NeuN (1:1000; ab279297, Abcam. .. The sections were rinsed with PBS and incubated for 2 hours with secondary antibodies: donkey anti-rabbit Alexa Fluor 488 (1:500; ab150064, Abcam), donkey anti-goat IgG Alexa Fluor 594 (ab150132, Abcam), donkey anti-goat IgG Alexa Fluor 488 (ab150129, Abcam), donkey anti-rat IgG Alexa Fluor 488 (Invitrogen, A21208), and donkey anti-rabbit IgG Alexa Fluor 594 (Abcam, ab150076).

    Article Title: The Calcium-Binding Protein S100A10 (p11) Is Required for Normal Motor Performance by Regulating Vesicle Dynamics at Excitatory Synapses.
    Article Snippet: .. After PBS washing, sections were immersed in a blocking solution [2.5% (w/v) bovine serum albumin and 0.1% (v/v) Triton X- 100 in PBS] for 30 min, followed by incubation (4°C, overnight) with the polyclonal primary antibodies against GFAP (1:250; Santa Cruz Biotechnology, Cat# sc58766, RRID:AB_783554) developed in mouse, p11 (1:100, R&D systems, Cat# AF2377, RRID:AB_2183469) developed in goat, vGLUT2 (1:2000, Millipore Bioscience Research Reagents, Cat# AB2251, RRID:AB_1587626) developed in guinea pig, Munc13- 1 (1:200; Synaptic systems, Goettingen, Germany, Cat# 126- 102, RRID:AB_887734), and Synaptophysin (Syn) (1:200; Zymed Laboratories, San Francisco, CA, Cat# 18- 0130) developed in rabbit. .. Next, the tissue was rinsed with PBS and incubated for 1 h at room temperature with Cy2- conjugated, Cy3- conjugated, and/or Cy5- conjugated anti- goat, anti- mouse, anti- guinea pig, and/or anti- rabbit IgGs (1:200; Jackson ImmunoResearch Laboratories) as secondary antibodies.

    Article Title: Bioadaptive liquid-infused multifunctional fibers for long-term neural recording via BDNF stabilization and enhanced neural interaction.
    Article Snippet: For blocking of nonspecific binding and permeabilization, the sections were incubated in 0.3% Triton X- 100 (Sigma- Aldrich) and 5% normal donkey serum (Abcam) solution for 1 hour. .. The sections were then incubated overnight at room temperature with primary antibodies: rabbit anti–MMP- 9 (1:100; ab228402, Abcam), goat Iba1 (1:500; ab5076, Abcam), goat anti- S100A10 (10 μg/ml; AF2377, R&D Systems), rat anti- C3 (1:500; NB200- 540, Novus Biologicals), rabbit anti- GFAP (1:250; ab68428, Abcam), and rat anti- NeuN (1:1000; ab279297, Abcam. .. The sections were rinsed with PBS and incubated for 2 hours with secondary antibodies: donkey anti- rabbit Alexa Fluor 488 (1:500; ab150064, Abcam), donkey anti- goat IgG Alexa Fluor 594 (ab150132, Abcam), donkey anti- goat IgG Alexa Fluor 488 (ab150129, Abcam), donkey anti- rat IgG Alexa Fluor 488 (Invitrogen, A21208), and donkey anti- rabbit IgG Alexa Fluor 594 (Abcam, ab150076).



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