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c3b/ic3b/c3c, mouse, mab 2/11  (Hycult Biotech)


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    Hycult Biotech c3b/ic3b/c3c, mouse, mab 2/11
    C3b/Ic3b/C3c, Mouse, Mab 2/11, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HM1025/C3b%2FiC3b%2FC3c%2C+Mouse%2C+mAb+2%2F11/custom%40hm1065%4038912583
    Average 94 stars, based on 54 article reviews
    c3b/ic3b/c3c, mouse, mab 2/11 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Hypoxia-induced complement component 3 promotes aggressive tumor growth in the glioblastoma microenvironment.
    Article Snippet: .. To examine the C3 expression we designed a custom multiplexed immunofluorescence detection panel using Alexa Fluors and antibodies targeting C3 (Hycult, HM1065, 1:150 dilution), Olig2 (R&D Systems, AF2418, 1:200 dilution) as a tumor marker, GFAP for detection of astrocytes (Abcam, ab4674, 1:400 dilution) and Nestin (Abcam, ab7659, 1:100 dilution) or CD31 (ProteinTech, 28083-1-A, 1.250) for detection of perivascular niches and macrophage and microglia marker F4/80 (Abcam, 28083-1-AP, 1:100). ..

    Immunofluorescence:

    Article Title: Hypoxia-induced complement component 3 promotes aggressive tumor growth in the glioblastoma microenvironment.
    Article Snippet: .. To examine the C3 expression we designed a custom multiplexed immunofluorescence detection panel using Alexa Fluors and antibodies targeting C3 (Hycult, HM1065, 1:150 dilution), Olig2 (R&D Systems, AF2418, 1:200 dilution) as a tumor marker, GFAP for detection of astrocytes (Abcam, ab4674, 1:400 dilution) and Nestin (Abcam, ab7659, 1:100 dilution) or CD31 (ProteinTech, 28083-1-A, 1.250) for detection of perivascular niches and macrophage and microglia marker F4/80 (Abcam, 28083-1-AP, 1:100). ..

    Marker:

    Article Title: Hypoxia-induced complement component 3 promotes aggressive tumor growth in the glioblastoma microenvironment.
    Article Snippet: .. To examine the C3 expression we designed a custom multiplexed immunofluorescence detection panel using Alexa Fluors and antibodies targeting C3 (Hycult, HM1065, 1:150 dilution), Olig2 (R&D Systems, AF2418, 1:200 dilution) as a tumor marker, GFAP for detection of astrocytes (Abcam, ab4674, 1:400 dilution) and Nestin (Abcam, ab7659, 1:100 dilution) or CD31 (ProteinTech, 28083-1-A, 1.250) for detection of perivascular niches and macrophage and microglia marker F4/80 (Abcam, 28083-1-AP, 1:100). ..

    Incubation:

    Article Title: CFD protein deficiency induce slow transit constipation is correlated with gut microbial dysbiosis
    Article Snippet: .. Then, incubated with rabbit anti-C3b, iC3b, C3c (1:300; Hycult, HM1065) at 4°C overnight. .. After rinsing in PBS, the sections were incubated with AF488-conju-gated goat anti-rabbit IgG H&L (1:500; Beyotime, A0423) at room temperature (RT) for 1 h. Subsequently, DAPI (Beyotime, C1005)stained the cell nucleus for 15 min, and after elution, it was observed under a fluorescence microscope.

    Article Title: CFD protein deficiency induce slow transit constipation is correlated with gut microbial dysbiosis.
    Article Snippet: .. Then, incubated with rabbit anti-C3b, iC3b, C3c (1:300; Hycult, HM1065) at 4°C overnight. .. After rinsing in PBS, the sections were incubated with AF488-conju-gated goat anti-rabbit IgG H&L (1:500; Beyotime, A0423) at room temperature (RT) for 1 h. Subsequently, DAPI (Beyotime, C1005)stained the cell nucleus for 15 minutes, and after elution, it was observed under a fluorescence microscope.

    Article Title: Brain-penetrant complement inhibition mitigates neurodegeneration in an Alzheimer’s disease mouse model
    Article Snippet: .. To measure mouse C3b/iC3b/C3c, plates were coated with 2/11 mAb anti-mouse C3b/iC3b/C3c (5 μg/ml, HM1065, Hycult Biotech), blocked in 3% BSA-PBS-T and washed in PBS-T. TBH or TBP were diluted 1:800, sera 1:20 000 in 0.3% BSA-PBS-T-EDTA, added in duplicate to ELISA wells, incubated overnight at 4°C, washed and bound C3 fragments detected using in-house HRP-labelled rabbit anti-human C3 (cross-reactive with mouse), 1:500 in 0.3% BSA-PBS-T-EDTA for 1.5 h at RT. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Brain-penetrant complement inhibition mitigates neurodegeneration in an Alzheimer’s disease mouse model
    Article Snippet: .. To measure mouse C3b/iC3b/C3c, plates were coated with 2/11 mAb anti-mouse C3b/iC3b/C3c (5 μg/ml, HM1065, Hycult Biotech), blocked in 3% BSA-PBS-T and washed in PBS-T. TBH or TBP were diluted 1:800, sera 1:20 000 in 0.3% BSA-PBS-T-EDTA, added in duplicate to ELISA wells, incubated overnight at 4°C, washed and bound C3 fragments detected using in-house HRP-labelled rabbit anti-human C3 (cross-reactive with mouse), 1:500 in 0.3% BSA-PBS-T-EDTA for 1.5 h at RT. ..



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