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anti mouse tlr4 md 2 mts510 mab  (Hycult Biotech)


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    Structured Review

    Hycult Biotech anti mouse tlr4 md 2 mts510 mab
    LPS-Trap-Fc1 to -Fc4 bind LPS and block LPS-mediated IL-6 production in Mono Mac 6 cells. (A) Supernatants of HEK293T cells transfected with LPS-Trap-Fc1 were incubated in the presence (lane 1) or absence (lane 3) of biotin-LPS. Additionally, supernatants were preincubated with an <t>anti-TLR4/MD-2</t> MAb (lane 2) or an excess of LPS (lane 4). Complexes were immunoprecipitated with streptavidin-Sepharose, subjected to SDS-PAGE, and analyzed by Western blotting with an anti-FLAG MAb. (B) RAW 264.7 cells were incubated with LPS-Trap-Fc1 or a medium control. Cells were stimulated with 100 ng/ml LPS overnight, and IL-6 levels in supernatants were determined by ELISA. *, P < 0.002 compared to control (Mann-Whitney U test, n = 6).
    Anti Mouse Tlr4 Md 2 Mts510 Mab, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HM1025/TLR4%2FMD-2%2C+Mouse%2C+mAb+MTS510/pmc02708546-155-29-33
    Average 90 stars, based on 11 article reviews
    anti mouse tlr4 md 2 mts510 mab - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Lipopolysaccharide-Trap-Fc, a Multifunctional Agent To Battle Gram-Negative Bacteria ▿ "

    Article Title: Lipopolysaccharide-Trap-Fc, a Multifunctional Agent To Battle Gram-Negative Bacteria ▿

    Journal:

    doi: 10.1128/IAI.00004-09

    LPS-Trap-Fc1 to -Fc4 bind LPS and block LPS-mediated IL-6 production in Mono Mac 6 cells. (A) Supernatants of HEK293T cells transfected with LPS-Trap-Fc1 were incubated in the presence (lane 1) or absence (lane 3) of biotin-LPS. Additionally, supernatants were preincubated with an anti-TLR4/MD-2 MAb (lane 2) or an excess of LPS (lane 4). Complexes were immunoprecipitated with streptavidin-Sepharose, subjected to SDS-PAGE, and analyzed by Western blotting with an anti-FLAG MAb. (B) RAW 264.7 cells were incubated with LPS-Trap-Fc1 or a medium control. Cells were stimulated with 100 ng/ml LPS overnight, and IL-6 levels in supernatants were determined by ELISA. *, P < 0.002 compared to control (Mann-Whitney U test, n = 6).
    Figure Legend Snippet: LPS-Trap-Fc1 to -Fc4 bind LPS and block LPS-mediated IL-6 production in Mono Mac 6 cells. (A) Supernatants of HEK293T cells transfected with LPS-Trap-Fc1 were incubated in the presence (lane 1) or absence (lane 3) of biotin-LPS. Additionally, supernatants were preincubated with an anti-TLR4/MD-2 MAb (lane 2) or an excess of LPS (lane 4). Complexes were immunoprecipitated with streptavidin-Sepharose, subjected to SDS-PAGE, and analyzed by Western blotting with an anti-FLAG MAb. (B) RAW 264.7 cells were incubated with LPS-Trap-Fc1 or a medium control. Cells were stimulated with 100 ng/ml LPS overnight, and IL-6 levels in supernatants were determined by ELISA. *, P < 0.002 compared to control (Mann-Whitney U test, n = 6).

    Techniques Used: Blocking Assay, Transfection, Incubation, Immunoprecipitation, SDS Page, Western Blot, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Related Articles

    other:

    Article Title: Candida albicans oscillating UME6 expression during intestinal colonization primes systemic Th17 protective immunity
    Article Snippet: Anti-mouse TLR4/MD2 (clone MTS510) , Hycult Biotech , Cat#HM1029; RRID:AB_533218.

    Article Title: Effect of Agaricus brasiliensis-derived cold water extract on Toll-like receptor 2-dependent cytokine production in vitro.
    Article Snippet: Agaricus brasiliensis (Agaricus blazei Murrill) is well known as a medicinal mushroom.. Fruit body of A. brasiliensis is rich in β-glucan and has shown benefits for various diseases.. Both hot and cold water extraction are traditional methods for intake of this mushroom extract.

    Article Title: Commensal fungi recapitulate the protective benefits of intestinal bacteria
    Article Snippet: Anti-mouse TLR4/MD2 (clone MTS510) , Hycult Biotech , Cat#HM1029-FS.

    Article Title: Maturation of dendritic cells by maitake α-glucan enhances anti-cancer effect of dendritic cell vaccination.
    Article Snippet: Dendritic cells (DCs) play a primary role in antigen presentation to CD4 and CD8 T cells and induce acquired immune response against cancer cells.. Therefore, determining positive modulators of DC activation to improve therapeutic approaches for cancer immunotherapy is greatly needed.. In this study, we investigated the effect of maitake α-glucan YM-2A, isolated from Grifola frondosa, on the maturation and function of DCs and its adjuvant effect on a tumor-associated antigen (TAA)-loaded DC vaccine against murine tumor.

    Infection:

    Article Title: Biliary atresia: Rotavirus amplification of lipopolysaccharide/toll-like receptor 4 by mediating MMP7 upregulation through NF-κB.
    Article Snippet: BACKGROUND: Matrix metalloproteinase-7 (MMP7), which is expressed primarily by biliary epithelial cells (BECs), has been shown to promote biliary atresia (BA).. However, the mechanism by which elevated MMP7 expression is induced in BA remains unclear.. METHODS: Mouse extrahepatic BECs were used to investigate MMP7 overexpression induced by rhesus rotavirus (RRV) and lipopolysaccharide (LPS).



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