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c3b/ic3b/c3c, mouse, mab 2/11  (Hycult Biotech)


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    Hycult Biotech c3b/ic3b/c3c, mouse, mab 2/11
    C3b/Ic3b/C3c, Mouse, Mab 2/11, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HM1025/C3b%2FiC3b%2FC3c%2C+Mouse%2C+mAb+2%2F11/custom%40hm1065%4038912583
    Average 94 stars, based on 57 article reviews
    c3b/ic3b/c3c, mouse, mab 2/11 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Cytometry:

    Article Title: The temporal gene expression landscape of rhabdomyolysis-induced acute kidney injury reveals the timing of complement activation
    Article Snippet: .. Antibody Species Clone Supplier Dilution Na+K+ ATPase (tubule cells) Rabbit EP1845Y Abcam 1:2000 CD34 (endothelial cells) Mouse QBend-10 Dako 1:100 C3c Rabbit Polyclonal Dako 1:500 C3d Rabbit Polyclonal Dako 1:1000 FH Rabbit Polyclonal ProteinTech 1:100 CD11b (CR3/myeloid cells) Rabbit EPR1244 Abcam 1:500 Antibody Species Clone Supplier Dilution Megalin (proximal tubule cells) Rabbit Abcam 1:2000 CD31 (endothelial cells) Goat Polyclonal Bio-Techne 1:50 C3b/iC3, Rat 2/11 Hycult 1:100 cCaspase3 (cell death) Rabbit 5A1E Cell Signaling Technology 1:100 CD11b (CR3/myeloid cells) Rabbit EPR1244 Abcam 1:500 5 Supplementary Table 6: Cytometry antibodies and life marker panel. ..

    Marker:

    Article Title: The temporal gene expression landscape of rhabdomyolysis-induced acute kidney injury reveals the timing of complement activation
    Article Snippet: .. Antibody Species Clone Supplier Dilution Na+K+ ATPase (tubule cells) Rabbit EP1845Y Abcam 1:2000 CD34 (endothelial cells) Mouse QBend-10 Dako 1:100 C3c Rabbit Polyclonal Dako 1:500 C3d Rabbit Polyclonal Dako 1:1000 FH Rabbit Polyclonal ProteinTech 1:100 CD11b (CR3/myeloid cells) Rabbit EPR1244 Abcam 1:500 Antibody Species Clone Supplier Dilution Megalin (proximal tubule cells) Rabbit Abcam 1:2000 CD31 (endothelial cells) Goat Polyclonal Bio-Techne 1:50 C3b/iC3, Rat 2/11 Hycult 1:100 cCaspase3 (cell death) Rabbit 5A1E Cell Signaling Technology 1:100 CD11b (CR3/myeloid cells) Rabbit EPR1244 Abcam 1:500 5 Supplementary Table 6: Cytometry antibodies and life marker panel. ..

    Incubation:

    Article Title: CFD protein deficiency induce slow transit constipation is correlated with gut microbial dysbiosis
    Article Snippet: .. Then, incubated with rabbit anti-C3b, iC3b, C3c (1:300; Hycult, HM1065) at 4°C overnight. .. After rinsing in PBS, the sections were incubated with AF488-conju-gated goat anti-rabbit IgG H&L (1:500; Beyotime, A0423) at room temperature (RT) for 1 h. Subsequently, DAPI (Beyotime, C1005)stained the cell nucleus for 15 min, and after elution, it was observed under a fluorescence microscope.

    Article Title: Tumor-derived neutrophil extracellular trap–associated DNA impairs treatment efficacy in breast cancer via CCDC25-dependent epithelial-mesenchymal transition
    Article Snippet: After boiling in 1× loading buffer (Cat# AM8547, Thermo ScientificTM), equivalent amount of protein was resolved by SDS-PAGE gels and transferred to PVDF membranes. .. Primary antibodies specific for MPO (1:2000, Cat# AF3667, R&D), H3cit (1:1000, Cat# ab281584, Abcam), C3a (1:2000, Cat# HM1072, Hycult Biotech), C5a (1:1000, Cat# MAB21501, R&D system), C3b/iC3b/C3c (1:2000, Cat# HM1065, Hycult Biotech), CCDC25 (1:1000, Cat# 21209-1-AP, ProteinTech), STAT3 (1:3000, Cat# Ab68153, Abcam), pTyr705-stat3 (1:2000, Cat# Ab76315, Abcam), SMAD2/3 (1:1000, Cat# 8685, CST), Phosphor SMAD2(Ser465/467)/SMAD3(Ser423/425) (1:1000, Cat# 8828, CST), Ecadherin (1:1000, Cat# 3195, CST), N-cadherin (1:2000, Cat# Ab207608, Abcam), Vimentin (1:2000, Cat# Ab92547, Abcam), TWIST1 (1:2000, Cat# Sc-81417, Santa Cruz), SNAIL (1:1000, Cat# 3879, CST), CTSC (1:1000, Cat# sc-74590, Santa Cruz), PKM2 (1:2000, Cat# 4053, CST), 6X His (1:2000, Cat# Ab18184, Abcam), Vinculin (1:3000, Cat# 18799, CST), GAPDH (1:3000, Cat# HRP-60004, ProteinTech) and βactin (1:3000, Cat# HRP-60008, ProteinTech) were diluted in TBST containing 5% BSA and incubated with membranes overnight at 4°C. .. The next day, peroxidaseconjugated secondary antibodies (1:3000, Cat# 7076, 7077, 7074, CST; Cat# SA000014, ProteinTech) were used, and the antigen-antibody reactions were visualized with enhanced chemiluminescence assays (ECL, Thermo ScientificTM).

    Article Title: CFD protein deficiency induce slow transit constipation is correlated with gut microbial dysbiosis.
    Article Snippet: .. Then, incubated with rabbit anti-C3b, iC3b, C3c (1:300; Hycult, HM1065) at 4°C overnight. .. After rinsing in PBS, the sections were incubated with AF488-conju-gated goat anti-rabbit IgG H&L (1:500; Beyotime, A0423) at room temperature (RT) for 1 h. Subsequently, DAPI (Beyotime, C1005)stained the cell nucleus for 15 minutes, and after elution, it was observed under a fluorescence microscope.

    Article Title: Dysregulation of complement at the synapse in P301S mice and human tauopathies.
    Article Snippet: Enzyme linked immunosorbent assay (ELISA): Nunc MaxiSorpTM flat-bottom 96-well plates (Invitrogen) were used for all ELISAs. .. For mouse iC3b ELISA, plates were coated with monoclonal antibody (mAb) 2/11 (rat anti-mouse C3b/iC3b; Hycult, HM1065), diluted to 5 μg/ml in carbonate buffer (35 mM NaHCO3, 15 mM Na2CO3, pH 9.6), and incubated overnight at 4oC. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Dysregulation of complement at the synapse in P301S mice and human tauopathies.
    Article Snippet: Enzyme linked immunosorbent assay (ELISA): Nunc MaxiSorpTM flat-bottom 96-well plates (Invitrogen) were used for all ELISAs. .. For mouse iC3b ELISA, plates were coated with monoclonal antibody (mAb) 2/11 (rat anti-mouse C3b/iC3b; Hycult, HM1065), diluted to 5 μg/ml in carbonate buffer (35 mM NaHCO3, 15 mM Na2CO3, pH 9.6), and incubated overnight at 4oC. ..



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