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dual luciferase assay  (BPS Bioscience)


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    Structured Review

    BPS Bioscience dual luciferase assay
    Dual Luciferase Assay, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/60683-1/Dual+Luciferase+(Firefly-Renilla)+Assay+System/pm40684724-305-13-23
    Average 94 stars, based on 54 article reviews
    dual luciferase assay - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Luciferase:

    Article Title: Modulation of Pulmonary Inflammation and the Redox Pathway In Vitro and In Vivo by Fumaric Ester.
    Article Snippet: After 24 h, cells were transfected with an Nrf2- or NF-κB-responsive luciferase reporter plasmid (BPS Bioscience, San Diego, CA, USA) using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) in Opti-MEM containing 1% FBS, following the manufacturer’s protocol. .. After stimulation, cells and culture medium were transferred to white 96-well plates, and luciferase activity was assessed using the Dual Luciferase Assay System (BPS Bioscience, San Diego, CA, USA) on a Varioskan LUX reader (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Modulation of Pulmonary Inflammation and the Redox Pathway In Vitro and In Vivo by Fumaric Ester
    Article Snippet: After 24 h, cells were transfected with an Nrf2- or NF-κB-responsive luciferase reporter plasmid (BPS Bioscience, San Diego, CA, USA) using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) in Opti-MEM containing 1% FBS, following the manufacturer’s protocol. .. After stimulation, cells and culture medium were transferred to white 96-well plates, and luciferase activity was assessed using the Dual Luciferase Assay System (BPS Bioscience, San Diego, CA, USA) on a Varioskan LUX reader (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Design, multicomponent synthesis, and molecular modelling studies of tetrazole biphenyls as REV-ERBα modulators.
    Article Snippet: The efficient synthesis of biphenyl tetrazole derivatives was achieved through a one-pot multicomponent reaction catalyzed by iron nanoparticles supported on silica (Fe/SiO2).. This green and sustainable approach offers significant advantages over conventional catalytic systems, including high yields (up to 92 %), mild reaction conditions (100 C), and excellent catalyst recyclability.. The methodology involves a Suzuki-Miyaura coupling reaction followed by an in situ [2 + 3] cycloaddition, with dimethylformamide (DMF) as the solvent.

    Article Title: Nrf2-dependent effects of CDDO-Me on bactericidal activity in macrophage infection models
    Article Snippet: Phorbol 12-myristate 13-acetate (PMA), human interferon gamma (IFNγ), lipopolysaccharide (LPS), dimethyl sulfoxide (DMSO), anti-GAPDH antibody, β-mercaptoethanol, and bardoxolone methyl (CDDO-Me) were purchased from Sigma (Merck). .. Dual Luciferase (Firefly-Renilla) Assay System was purchased from BPS Bioscience. .. DMEM (Dulbecco’s Modified Eagle Medium), RPMI (Roswell Park Memorial Institute) 1640 culture medium, phosphate-buffered saline (PBS), and heat-inactivated fetal bovine serum were obtained from Eurobio Scientific.

    Article Title: TWEAK-induced alternative NF-κB signaling regulates Cxcl10 and Mymx to promote myoblast-to-myotube fusion and muscle regeneration.
    Article Snippet: .. Luciferase reporter activity was quantified following cell lysis using the TWO-Step Luciferase (Firefly and Renilla) Assay System (60683, BPS Bioscience) following manufacturer’s protocol. .. Luciferase activity was detected using the BioTek Synergy HTX Multimode Reader (Agilent).

    Article Title: Development of novel quinazolinone-based compounds with anti-proliferative activity as dual STAT-3 and c-Src inhibitors: design, synthesis and molecular dynamic studies.
    Article Snippet: A series of quinazolinone derivatives 5a, 5b, 6a–f, 7a–c, 8a–e, and 12a–d with different moieties as dual STAT3/c-Src inhibitors were designed, synthesized, and evaluated for anti-cancer activity.. In vitro anti-cancer screening was carried out against 60 human cancer cell lines by (NCI) for all the synthesized compounds 5a, 5b, 6a–f, 7a–c, 8a–e, and 12a–d.. All the new compounds showed anti-cancer inhibitory activity against various human cell lines.

    Activity Assay:

    Article Title: Modulation of Pulmonary Inflammation and the Redox Pathway In Vitro and In Vivo by Fumaric Ester.
    Article Snippet: After 24 h, cells were transfected with an Nrf2- or NF-κB-responsive luciferase reporter plasmid (BPS Bioscience, San Diego, CA, USA) using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) in Opti-MEM containing 1% FBS, following the manufacturer’s protocol. .. After stimulation, cells and culture medium were transferred to white 96-well plates, and luciferase activity was assessed using the Dual Luciferase Assay System (BPS Bioscience, San Diego, CA, USA) on a Varioskan LUX reader (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Modulation of Pulmonary Inflammation and the Redox Pathway In Vitro and In Vivo by Fumaric Ester
    Article Snippet: After 24 h, cells were transfected with an Nrf2- or NF-κB-responsive luciferase reporter plasmid (BPS Bioscience, San Diego, CA, USA) using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) in Opti-MEM containing 1% FBS, following the manufacturer’s protocol. .. After stimulation, cells and culture medium were transferred to white 96-well plates, and luciferase activity was assessed using the Dual Luciferase Assay System (BPS Bioscience, San Diego, CA, USA) on a Varioskan LUX reader (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: TWEAK-induced alternative NF-κB signaling regulates Cxcl10 and Mymx to promote myoblast-to-myotube fusion and muscle regeneration.
    Article Snippet: .. Luciferase reporter activity was quantified following cell lysis using the TWO-Step Luciferase (Firefly and Renilla) Assay System (60683, BPS Bioscience) following manufacturer’s protocol. .. Luciferase activity was detected using the BioTek Synergy HTX Multimode Reader (Agilent).

    Incubation:

    Article Title: Design, multicomponent synthesis, and molecular modelling studies of tetrazole biphenyls as REV-ERBα modulators.
    Article Snippet: The efficient synthesis of biphenyl tetrazole derivatives was achieved through a one-pot multicomponent reaction catalyzed by iron nanoparticles supported on silica (Fe/SiO2).. This green and sustainable approach offers significant advantages over conventional catalytic systems, including high yields (up to 92 %), mild reaction conditions (100 C), and excellent catalyst recyclability.. The methodology involves a Suzuki-Miyaura coupling reaction followed by an in situ [2 + 3] cycloaddition, with dimethylformamide (DMF) as the solvent.

    Lysis:

    Article Title: TWEAK-induced alternative NF-κB signaling regulates Cxcl10 and Mymx to promote myoblast-to-myotube fusion and muscle regeneration.
    Article Snippet: .. Luciferase reporter activity was quantified following cell lysis using the TWO-Step Luciferase (Firefly and Renilla) Assay System (60683, BPS Bioscience) following manufacturer’s protocol. .. Luciferase activity was detected using the BioTek Synergy HTX Multimode Reader (Agilent).

    Reporter Assay:

    Article Title: Design, multicomponent synthesis, and molecular modelling studies of tetrazole biphenyls as REV-ERBα modulators.
    Article Snippet: The efficient synthesis of biphenyl tetrazole derivatives was achieved through a one-pot multicomponent reaction catalyzed by iron nanoparticles supported on silica (Fe/SiO2).. This green and sustainable approach offers significant advantages over conventional catalytic systems, including high yields (up to 92 %), mild reaction conditions (100 C), and excellent catalyst recyclability.. The methodology involves a Suzuki-Miyaura coupling reaction followed by an in situ [2 + 3] cycloaddition, with dimethylformamide (DMF) as the solvent.



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