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fcγ chain adcc jurkat  (BPS Bioscience)


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    BPS Bioscience fcγ chain adcc jurkat
    Fcγ Chain Adcc Jurkat, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/60541/ADCC+Bioassay+Effector+Cell+V+variant+(High+Affinity)+-+Jurkat+Recombinant+Cell+Line/pm38968862-58-26-30
    Average 93 stars, based on 10 article reviews
    fcγ chain adcc jurkat - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Bioassay:

    Article Title: Optimization of a U937 cell-based reporter system to assess ADCC activity of ustekinumab versus trastuzumab.
    Article Snippet: In the present study, the human monocytic U937 cell line, which intrinsically expresses low levels of membranebound IL-12, was systematically optimized to establish a robust target cell system for a surrogate ADCC reporter assay aimed at evaluating the effector function of ustekinumab.. ADCC activity was assessed in parallel with trastuzumab, employed as a well-characterized positive control, using a bioluminescent reporter-based platform.. To induce surface IL-12 overexpression, U937 cells were stimulated with varying concentrations of IFN-γ and lipopolysaccharide (LPS), and expression levels were quantitatively assessed by flow cytometry using FITClabelled antibody staining, alongside cell viability evaluation via trypan blue exclusion.

    Article Title: Antibodies and methods for making and using the same
    Article Snippet: .. Effector Cell line—ADCC Bioassay effector cell line V variant (BPS Biosciences #60541) 4. ..

    Variant Assay:

    Article Title: Optimization of a U937 cell-based reporter system to assess ADCC activity of ustekinumab versus trastuzumab.
    Article Snippet: In the present study, the human monocytic U937 cell line, which intrinsically expresses low levels of membranebound IL-12, was systematically optimized to establish a robust target cell system for a surrogate ADCC reporter assay aimed at evaluating the effector function of ustekinumab.. ADCC activity was assessed in parallel with trastuzumab, employed as a well-characterized positive control, using a bioluminescent reporter-based platform.. To induce surface IL-12 overexpression, U937 cells were stimulated with varying concentrations of IFN-γ and lipopolysaccharide (LPS), and expression levels were quantitatively assessed by flow cytometry using FITClabelled antibody staining, alongside cell viability evaluation via trypan blue exclusion.

    Article Title: Antibodies and methods for making and using the same
    Article Snippet: .. Effector Cell line—ADCC Bioassay effector cell line V variant (BPS Biosciences #60541) 4. ..

    Article Title: Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma
    Article Snippet: .. Recombinant Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMC) were separated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from blood donated by healthy donors with written informed consent.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells
    Article Snippet: All PEL/MM/mantle cell lymphoma cell lines were grown in RPMI1640 (Fujifilm Wako, Osaka, Japan) supplemented with 10% FBS (Nichirei Bioscience, Tokyo, Japan), 100 IU/ml penicillin and 100 U/ml streptomycin at 37 °C, 5% CO 2 in a humidified atmosphere [ 24 ]. .. Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMCs) were isolated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from whole blood donated by healthy volunteer donors with written informed consent.

    Article Title: Trastuzumab, a monoclonal anti-HER2 antibody modulates cytotoxicity against cholangiocarcinoma via multiple mechanisms.
    Article Snippet: Cholangiocarcinoma (CCA) is an aggressive and fatal cancer.. The prognosis is very poor and no optimal chemotherapy has been established.. Human epidermal growth factor receptor 2 (HER2, neu, and erbB2) is highly-expressed in breast cancer and is expressed in many other tumors but poorly expressed in CCA.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells.
    Article Snippet: Primary effusion lymphoma (PEL) is a rare aggressive B-cell non-Hodgkin’s lymphoma with no optimal treatment.. Signaling lymphocytic activation molecule-F7 (SLAMF7, CD319), a type I transmembrane glycoprotein highly expressed in multiple myeloma (MM), represents a promising target for mAb-based immunotherapy.. SLAMF7 also expresses on several hematopoietic lineages including NK cells.

    Recombinant:

    Article Title: Optimization of a U937 cell-based reporter system to assess ADCC activity of ustekinumab versus trastuzumab.
    Article Snippet: In the present study, the human monocytic U937 cell line, which intrinsically expresses low levels of membranebound IL-12, was systematically optimized to establish a robust target cell system for a surrogate ADCC reporter assay aimed at evaluating the effector function of ustekinumab.. ADCC activity was assessed in parallel with trastuzumab, employed as a well-characterized positive control, using a bioluminescent reporter-based platform.. To induce surface IL-12 overexpression, U937 cells were stimulated with varying concentrations of IFN-γ and lipopolysaccharide (LPS), and expression levels were quantitatively assessed by flow cytometry using FITClabelled antibody staining, alongside cell viability evaluation via trypan blue exclusion.

    Article Title: Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma
    Article Snippet: .. Recombinant Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMC) were separated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from blood donated by healthy donors with written informed consent.

    Article Title: Trastuzumab, a monoclonal anti-HER2 antibody modulates cytotoxicity against cholangiocarcinoma via multiple mechanisms.
    Article Snippet: Cholangiocarcinoma (CCA) is an aggressive and fatal cancer.. The prognosis is very poor and no optimal chemotherapy has been established.. Human epidermal growth factor receptor 2 (HER2, neu, and erbB2) is highly-expressed in breast cancer and is expressed in many other tumors but poorly expressed in CCA.

    Article Title: Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma.
    Article Snippet: Primary effusion lymphoma (PEL) is a rare, aggressive B cell non-Hodgkin’s lymphoma of the body cavities with malignant effusions.. The prognosis is poor, and no optimal treatment has been established.. CD38 is a type II transmembrane glycoprotein known to overexpress in multiple myeloma (MM).

    Expressing:

    Article Title: Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma
    Article Snippet: .. Recombinant Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMC) were separated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from blood donated by healthy donors with written informed consent.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells
    Article Snippet: All PEL/MM/mantle cell lymphoma cell lines were grown in RPMI1640 (Fujifilm Wako, Osaka, Japan) supplemented with 10% FBS (Nichirei Bioscience, Tokyo, Japan), 100 IU/ml penicillin and 100 U/ml streptomycin at 37 °C, 5% CO 2 in a humidified atmosphere [ 24 ]. .. Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMCs) were isolated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from whole blood donated by healthy volunteer donors with written informed consent.

    Article Title: Trastuzumab, a monoclonal anti-HER2 antibody modulates cytotoxicity against cholangiocarcinoma via multiple mechanisms.
    Article Snippet: Cholangiocarcinoma (CCA) is an aggressive and fatal cancer.. The prognosis is very poor and no optimal chemotherapy has been established.. Human epidermal growth factor receptor 2 (HER2, neu, and erbB2) is highly-expressed in breast cancer and is expressed in many other tumors but poorly expressed in CCA.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells.
    Article Snippet: Primary effusion lymphoma (PEL) is a rare aggressive B-cell non-Hodgkin’s lymphoma with no optimal treatment.. Signaling lymphocytic activation molecule-F7 (SLAMF7, CD319), a type I transmembrane glycoprotein highly expressed in multiple myeloma (MM), represents a promising target for mAb-based immunotherapy.. SLAMF7 also expresses on several hematopoietic lineages including NK cells.

    Luciferase:

    Article Title: Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma
    Article Snippet: .. Recombinant Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMC) were separated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from blood donated by healthy donors with written informed consent.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells
    Article Snippet: All PEL/MM/mantle cell lymphoma cell lines were grown in RPMI1640 (Fujifilm Wako, Osaka, Japan) supplemented with 10% FBS (Nichirei Bioscience, Tokyo, Japan), 100 IU/ml penicillin and 100 U/ml streptomycin at 37 °C, 5% CO 2 in a humidified atmosphere [ 24 ]. .. Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMCs) were isolated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from whole blood donated by healthy volunteer donors with written informed consent.

    Article Title: Trastuzumab, a monoclonal anti-HER2 antibody modulates cytotoxicity against cholangiocarcinoma via multiple mechanisms.
    Article Snippet: Cholangiocarcinoma (CCA) is an aggressive and fatal cancer.. The prognosis is very poor and no optimal chemotherapy has been established.. Human epidermal growth factor receptor 2 (HER2, neu, and erbB2) is highly-expressed in breast cancer and is expressed in many other tumors but poorly expressed in CCA.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells.
    Article Snippet: Primary effusion lymphoma (PEL) is a rare aggressive B-cell non-Hodgkin’s lymphoma with no optimal treatment.. Signaling lymphocytic activation molecule-F7 (SLAMF7, CD319), a type I transmembrane glycoprotein highly expressed in multiple myeloma (MM), represents a promising target for mAb-based immunotherapy.. SLAMF7 also expresses on several hematopoietic lineages including NK cells.

    Control:

    Article Title: Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma
    Article Snippet: .. Recombinant Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMC) were separated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from blood donated by healthy donors with written informed consent.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells
    Article Snippet: All PEL/MM/mantle cell lymphoma cell lines were grown in RPMI1640 (Fujifilm Wako, Osaka, Japan) supplemented with 10% FBS (Nichirei Bioscience, Tokyo, Japan), 100 IU/ml penicillin and 100 U/ml streptomycin at 37 °C, 5% CO 2 in a humidified atmosphere [ 24 ]. .. Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMCs) were isolated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from whole blood donated by healthy volunteer donors with written informed consent.

    Article Title: Trastuzumab, a monoclonal anti-HER2 antibody modulates cytotoxicity against cholangiocarcinoma via multiple mechanisms.
    Article Snippet: Cholangiocarcinoma (CCA) is an aggressive and fatal cancer.. The prognosis is very poor and no optimal chemotherapy has been established.. Human epidermal growth factor receptor 2 (HER2, neu, and erbB2) is highly-expressed in breast cancer and is expressed in many other tumors but poorly expressed in CCA.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells.
    Article Snippet: Primary effusion lymphoma (PEL) is a rare aggressive B-cell non-Hodgkin’s lymphoma with no optimal treatment.. Signaling lymphocytic activation molecule-F7 (SLAMF7, CD319), a type I transmembrane glycoprotein highly expressed in multiple myeloma (MM), represents a promising target for mAb-based immunotherapy.. SLAMF7 also expresses on several hematopoietic lineages including NK cells.

    Activity Assay:

    Article Title: Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma
    Article Snippet: .. Recombinant Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMC) were separated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from blood donated by healthy donors with written informed consent.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells
    Article Snippet: All PEL/MM/mantle cell lymphoma cell lines were grown in RPMI1640 (Fujifilm Wako, Osaka, Japan) supplemented with 10% FBS (Nichirei Bioscience, Tokyo, Japan), 100 IU/ml penicillin and 100 U/ml streptomycin at 37 °C, 5% CO 2 in a humidified atmosphere [ 24 ]. .. Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMCs) were isolated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from whole blood donated by healthy volunteer donors with written informed consent.

    Article Title: Trastuzumab, a monoclonal anti-HER2 antibody modulates cytotoxicity against cholangiocarcinoma via multiple mechanisms.
    Article Snippet: Cholangiocarcinoma (CCA) is an aggressive and fatal cancer.. The prognosis is very poor and no optimal chemotherapy has been established.. Human epidermal growth factor receptor 2 (HER2, neu, and erbB2) is highly-expressed in breast cancer and is expressed in many other tumors but poorly expressed in CCA.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells.
    Article Snippet: Primary effusion lymphoma (PEL) is a rare aggressive B-cell non-Hodgkin’s lymphoma with no optimal treatment.. Signaling lymphocytic activation molecule-F7 (SLAMF7, CD319), a type I transmembrane glycoprotein highly expressed in multiple myeloma (MM), represents a promising target for mAb-based immunotherapy.. SLAMF7 also expresses on several hematopoietic lineages including NK cells.

    Reporter Assay:

    Article Title: Efficacy and mechanism of the anti-CD38 monoclonal antibody Daratumumab against primary effusion lymphoma
    Article Snippet: .. Recombinant Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMC) were separated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from blood donated by healthy donors with written informed consent.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells
    Article Snippet: All PEL/MM/mantle cell lymphoma cell lines were grown in RPMI1640 (Fujifilm Wako, Osaka, Japan) supplemented with 10% FBS (Nichirei Bioscience, Tokyo, Japan), 100 IU/ml penicillin and 100 U/ml streptomycin at 37 °C, 5% CO 2 in a humidified atmosphere [ 24 ]. .. Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, high affinity (V158) variant and Fcγ chain (ADCC-Jurkat) (#60,541, BPS Bioscience, San Diego, CA) were used to measure ADCC activity by reporter assay. .. Peripheral blood mononuclear cells (PBMCs) were isolated using Pancoll Human (PAN Biotech, Geschäftszeiten, Germany) from whole blood donated by healthy volunteer donors with written informed consent.

    Article Title: Trastuzumab, a monoclonal anti-HER2 antibody modulates cytotoxicity against cholangiocarcinoma via multiple mechanisms.
    Article Snippet: Cholangiocarcinoma (CCA) is an aggressive and fatal cancer.. The prognosis is very poor and no optimal chemotherapy has been established.. Human epidermal growth factor receptor 2 (HER2, neu, and erbB2) is highly-expressed in breast cancer and is expressed in many other tumors but poorly expressed in CCA.

    Article Title: Elotuzumab, a potential therapeutic humanized anti-SLAMF7 monoclonal antibody, enhances natural killer cell-mediated killing of primary effusion lymphoma cells.
    Article Snippet: Primary effusion lymphoma (PEL) is a rare aggressive B-cell non-Hodgkin’s lymphoma with no optimal treatment.. Signaling lymphocytic activation molecule-F7 (SLAMF7, CD319), a type I transmembrane glycoprotein highly expressed in multiple myeloma (MM), represents a promising target for mAb-based immunotherapy.. SLAMF7 also expresses on several hematopoietic lineages including NK cells.



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