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plasmid pice rnase hi wtnls mcherry  (Addgene inc)


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    Structured Review

    Addgene inc plasmid pice rnase hi wtnls mcherry
    Plasmid Pice Rnase Hi Wtnls Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/60365/pICE-RNaseHI-WT-NLS-mCherry+(Plasmid+%2360365)/pm37819055-61-8-19
    Average 93 stars, based on 20 article reviews
    plasmid pice rnase hi wtnls mcherry - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Live-cell imaging unveils distinct R-loop populations with heterogeneous dynamics.
    Article Snippet: .. The RNase H1 was PCR amplified from the plasmid pICE-RNase HI-WTNLS-mCherry (pICE-RNase HI-WT-NLS-mCherry was a gift from Patrick Calsou, Addgene plasmid #60365; http://n2t.net/ addgene:60365 ; RRID: Addgene_60365), with primers with BshTI and Acc65I extensions using the NZYProof DNA polymerase (NZYTech, Lisbon Portugal). ..

    Article Title: Live-cell imaging unveils distinct R-loop populations with heterogeneous dynamics
    Article Snippet: .. The RNase H1 was PCR amplified from the plasmid pICE-RNase HI-WT-NLS-mCherry (pICE-RNase HI-WT-NLS-mCherry was a gift from Patrick Calsou, Addgene plasmid #60365; http://n2t.net/addgene:60365 ; RRID: Addgene_60365), with primers with BshTI and Acc65I extensions using the NZYProof DNA polymerase (NZYTech, Lisbon Portugal). .. The RNase H1 was PCR amplified from the plasmid pICE-RNase HI-WT-NLS-mCherry (pICE-RNase HI-WT-NLS-mCherry was a gift from Patrick Calsou, Addgene plasmid #60365; http://n2t.net/addgene:60365 ; RRID: Addgene_60365), with primers with BshTI and Acc65I extensions using the NZYProof DNA polymerase (NZYTech, Lisbon Portugal).

    Amplification:

    Article Title: Live-cell imaging unveils distinct R-loop populations with heterogeneous dynamics.
    Article Snippet: .. The RNase H1 was PCR amplified from the plasmid pICE-RNase HI-WTNLS-mCherry (pICE-RNase HI-WT-NLS-mCherry was a gift from Patrick Calsou, Addgene plasmid #60365; http://n2t.net/ addgene:60365 ; RRID: Addgene_60365), with primers with BshTI and Acc65I extensions using the NZYProof DNA polymerase (NZYTech, Lisbon Portugal). ..

    Article Title: Live-cell imaging unveils distinct R-loop populations with heterogeneous dynamics
    Article Snippet: .. The RNase H1 was PCR amplified from the plasmid pICE-RNase HI-WT-NLS-mCherry (pICE-RNase HI-WT-NLS-mCherry was a gift from Patrick Calsou, Addgene plasmid #60365; http://n2t.net/addgene:60365 ; RRID: Addgene_60365), with primers with BshTI and Acc65I extensions using the NZYProof DNA polymerase (NZYTech, Lisbon Portugal). .. The RNase H1 was PCR amplified from the plasmid pICE-RNase HI-WT-NLS-mCherry (pICE-RNase HI-WT-NLS-mCherry was a gift from Patrick Calsou, Addgene plasmid #60365; http://n2t.net/addgene:60365 ; RRID: Addgene_60365), with primers with BshTI and Acc65I extensions using the NZYProof DNA polymerase (NZYTech, Lisbon Portugal).

    Plasmid Preparation:

    Article Title: Live-cell imaging unveils distinct R-loop populations with heterogeneous dynamics.
    Article Snippet: .. The RNase H1 was PCR amplified from the plasmid pICE-RNase HI-WTNLS-mCherry (pICE-RNase HI-WT-NLS-mCherry was a gift from Patrick Calsou, Addgene plasmid #60365; http://n2t.net/ addgene:60365 ; RRID: Addgene_60365), with primers with BshTI and Acc65I extensions using the NZYProof DNA polymerase (NZYTech, Lisbon Portugal). ..

    Article Title: Live-cell imaging unveils distinct R-loop populations with heterogeneous dynamics
    Article Snippet: .. The RNase H1 was PCR amplified from the plasmid pICE-RNase HI-WT-NLS-mCherry (pICE-RNase HI-WT-NLS-mCherry was a gift from Patrick Calsou, Addgene plasmid #60365; http://n2t.net/addgene:60365 ; RRID: Addgene_60365), with primers with BshTI and Acc65I extensions using the NZYProof DNA polymerase (NZYTech, Lisbon Portugal). .. The RNase H1 was PCR amplified from the plasmid pICE-RNase HI-WT-NLS-mCherry (pICE-RNase HI-WT-NLS-mCherry was a gift from Patrick Calsou, Addgene plasmid #60365; http://n2t.net/addgene:60365 ; RRID: Addgene_60365), with primers with BshTI and Acc65I extensions using the NZYProof DNA polymerase (NZYTech, Lisbon Portugal).

    Article Title: Chromatin compartmentalization regulates the response to DNA damage
    Article Snippet: For ATM or DNA-PK inhibition, cells were respectively pretreated for 1 h with 20 μM KU-55933 (Sigma-Aldrich, SML1109) or 2 μM NU-7441 (Selleckchem, S2638) and during subsequent OHT treatment. .. For cell synchronization, cells were incubated for 18 h with 2 mM thymidine (Sigma-Aldrich, T1895), then released during 11 h, followed by a second thymidine treatment for 18 h. S, G2 and G1 cells were then respectively treated with OHT at 0, 6 or 11 h after thymidine release and collected 4 h later. siRNA and plasmid transfections were performed using the 4D-Nucleofector kit and the SE cell line 4D-Nucleofector X L or S kit (Lonza) according to the manufacturer’s instructions, and subsequent treatment(s) were performed 48 h later. siRNA transfections were performed using a control siRNA (siCtrl): CAUGUCAUGUGUCACAUCU; or siRNA targeting SCC1 (siSCC1): GGUGAAAAUGGCAUUACGG; SMC1 (siSMC1): UAGGCUUCCUGGAGGUCACAUUUAA; 53BP1 (si53BP1): GAACGAGGAGACGGUAAUA; SUN2 (siSUN2): CGAGCCTATTCAGACGTTTCA; ARP2 (siARP2): GGCACCGGGUUUGUGAAGU; SETX (siSETX): GAGAGAAUUAUUGCGUACU; and RNASEH1 (siRNASEH1) : CUGUCUUGCUGCCUGUACU. pICE-NLS-mCherry (referred to as pICE-empty in the text; Addgene plasmid, 60364) and pICE-RNase-H1-WT-NLS-mCherry (referred to as pICE-RNase-H1 in the text, Addgene plasmid, 60365) were respectively used as control and for RNase H1 over-expression (gifts from P. Calsou and S. Britton). ..

    Article Title: Chromatin compartmentalization regulates the response to DNA damage.
    Article Snippet: For ATM or DNA-PK inhibition, cells were respectively pretreated for 1 h with 20 μM KU-55933 (Sigma-Aldrich, SML1109) or 2 μM NU-7441 (Selleckchem, S2638) and during subsequent OHT treatment. .. For cell synchronization, cells were incubated for 18 h with 2 mM thymidine (Sigma-Aldrich, T1895), then released during 11 h, followed by a second thymidine treatment for 18 h. S, G2 and G1 cells were then respectively treated with OHT at 0, 6 or 11 h after thymidine release and collected 4 h later. siRNA and plasmid transfections were performed using the 4D-Nucleofector kit and the SE cell line 4D-Nucleofector X L or S kit (Lonza) according to the manufacturer’s instructions, and subsequent treatment(s) were performed 48 h later. siRNA transfections were performed using a control siRNA (siCtrl): CAUGUCAUGUGUCACAUCU; or siRNA targeting SCC1 (siSCC1): GGUGAAAAUGGCAUUACGG; SMC1 (siSMC1): UAGG CUUCCUGGAGGUCACAUUUAA; 53BP1 (si53BP1): GAACGAGGAGA CGGUAAUA; SUN2 (siSUN2): CGAGCCTATTCAGACGTTTCA; ARP2 (siARP2): GGCACCGGGUUUGUGAAGU; SETX (siSETX): GAGAGAA UUAUUGCGUACU; and RNASEH1 (siRNASEH1)48: CUGUCUUGCUGC CUGUACU. pICE-NLS-mCherry (referred to as pICE-empty in the text; Addgene plasmid, 60364) and pICE-RNase-H1-WT-NLS-mCherry (referred to as pICE-RNase-H1 in the text, Addgene plasmid, 60365) were respectively used as control and for RNase H1 over-expression (gifts from P. Calsou and S. Britton). ..

    Over Expression:

    Article Title: Complex interplay between FMRP and DHX9 during DNA replication stress.
    Article Snippet: .. RNase HI overexpression. pICE-RNaseHI-NLS-mCherry (Addgene #60365), or pICE-RNaseHID10R-E48R-NLS-mCherry (Addgene #60367), or an empty vector without RNase HI was transfected into FMR1KO-B3-FMRP-H9 and FMR1KO-B3-FMRPI304N-F10 cells using TransIT2020 transfection reagent (Mirius Bio) following manufacturer’s instruction. ..

    Transfection:

    Article Title: Complex interplay between FMRP and DHX9 during DNA replication stress.
    Article Snippet: .. RNase HI overexpression. pICE-RNaseHI-NLS-mCherry (Addgene #60365), or pICE-RNaseHID10R-E48R-NLS-mCherry (Addgene #60367), or an empty vector without RNase HI was transfected into FMR1KO-B3-FMRP-H9 and FMR1KO-B3-FMRPI304N-F10 cells using TransIT2020 transfection reagent (Mirius Bio) following manufacturer’s instruction. ..

    Article Title: Complex interplay between FMRP and DHX9 during DNA replication stress
    Article Snippet: .. pICE-RNaseHI-NLS-mCherry (Addgene #60365), or pICE-RNaseHI-D10R-E48R-NLS-mCherry (Addgene #60367), or an empty vector without RNase HI was transfected into FMR1KO-B3-FMRP-H9 and FMR1KO-B3-FMRPI304N-F10 cells using TransIT-2020 transfection reagent (Mirus Bio) following manufacturer’s instruction. ..

    Article Title: Chromatin compartmentalization regulates the response to DNA damage
    Article Snippet: For ATM or DNA-PK inhibition, cells were respectively pretreated for 1 h with 20 μM KU-55933 (Sigma-Aldrich, SML1109) or 2 μM NU-7441 (Selleckchem, S2638) and during subsequent OHT treatment. .. For cell synchronization, cells were incubated for 18 h with 2 mM thymidine (Sigma-Aldrich, T1895), then released during 11 h, followed by a second thymidine treatment for 18 h. S, G2 and G1 cells were then respectively treated with OHT at 0, 6 or 11 h after thymidine release and collected 4 h later. siRNA and plasmid transfections were performed using the 4D-Nucleofector kit and the SE cell line 4D-Nucleofector X L or S kit (Lonza) according to the manufacturer’s instructions, and subsequent treatment(s) were performed 48 h later. siRNA transfections were performed using a control siRNA (siCtrl): CAUGUCAUGUGUCACAUCU; or siRNA targeting SCC1 (siSCC1): GGUGAAAAUGGCAUUACGG; SMC1 (siSMC1): UAGGCUUCCUGGAGGUCACAUUUAA; 53BP1 (si53BP1): GAACGAGGAGACGGUAAUA; SUN2 (siSUN2): CGAGCCTATTCAGACGTTTCA; ARP2 (siARP2): GGCACCGGGUUUGUGAAGU; SETX (siSETX): GAGAGAAUUAUUGCGUACU; and RNASEH1 (siRNASEH1) : CUGUCUUGCUGCCUGUACU. pICE-NLS-mCherry (referred to as pICE-empty in the text; Addgene plasmid, 60364) and pICE-RNase-H1-WT-NLS-mCherry (referred to as pICE-RNase-H1 in the text, Addgene plasmid, 60365) were respectively used as control and for RNase H1 over-expression (gifts from P. Calsou and S. Britton). ..

    Article Title: Chromatin compartmentalization regulates the response to DNA damage.
    Article Snippet: For ATM or DNA-PK inhibition, cells were respectively pretreated for 1 h with 20 μM KU-55933 (Sigma-Aldrich, SML1109) or 2 μM NU-7441 (Selleckchem, S2638) and during subsequent OHT treatment. .. For cell synchronization, cells were incubated for 18 h with 2 mM thymidine (Sigma-Aldrich, T1895), then released during 11 h, followed by a second thymidine treatment for 18 h. S, G2 and G1 cells were then respectively treated with OHT at 0, 6 or 11 h after thymidine release and collected 4 h later. siRNA and plasmid transfections were performed using the 4D-Nucleofector kit and the SE cell line 4D-Nucleofector X L or S kit (Lonza) according to the manufacturer’s instructions, and subsequent treatment(s) were performed 48 h later. siRNA transfections were performed using a control siRNA (siCtrl): CAUGUCAUGUGUCACAUCU; or siRNA targeting SCC1 (siSCC1): GGUGAAAAUGGCAUUACGG; SMC1 (siSMC1): UAGG CUUCCUGGAGGUCACAUUUAA; 53BP1 (si53BP1): GAACGAGGAGA CGGUAAUA; SUN2 (siSUN2): CGAGCCTATTCAGACGTTTCA; ARP2 (siARP2): GGCACCGGGUUUGUGAAGU; SETX (siSETX): GAGAGAA UUAUUGCGUACU; and RNASEH1 (siRNASEH1)48: CUGUCUUGCUGC CUGUACU. pICE-NLS-mCherry (referred to as pICE-empty in the text; Addgene plasmid, 60364) and pICE-RNase-H1-WT-NLS-mCherry (referred to as pICE-RNase-H1 in the text, Addgene plasmid, 60365) were respectively used as control and for RNase H1 over-expression (gifts from P. Calsou and S. Britton). ..

    Incubation:

    Article Title: Chromatin compartmentalization regulates the response to DNA damage
    Article Snippet: For ATM or DNA-PK inhibition, cells were respectively pretreated for 1 h with 20 μM KU-55933 (Sigma-Aldrich, SML1109) or 2 μM NU-7441 (Selleckchem, S2638) and during subsequent OHT treatment. .. For cell synchronization, cells were incubated for 18 h with 2 mM thymidine (Sigma-Aldrich, T1895), then released during 11 h, followed by a second thymidine treatment for 18 h. S, G2 and G1 cells were then respectively treated with OHT at 0, 6 or 11 h after thymidine release and collected 4 h later. siRNA and plasmid transfections were performed using the 4D-Nucleofector kit and the SE cell line 4D-Nucleofector X L or S kit (Lonza) according to the manufacturer’s instructions, and subsequent treatment(s) were performed 48 h later. siRNA transfections were performed using a control siRNA (siCtrl): CAUGUCAUGUGUCACAUCU; or siRNA targeting SCC1 (siSCC1): GGUGAAAAUGGCAUUACGG; SMC1 (siSMC1): UAGGCUUCCUGGAGGUCACAUUUAA; 53BP1 (si53BP1): GAACGAGGAGACGGUAAUA; SUN2 (siSUN2): CGAGCCTATTCAGACGTTTCA; ARP2 (siARP2): GGCACCGGGUUUGUGAAGU; SETX (siSETX): GAGAGAAUUAUUGCGUACU; and RNASEH1 (siRNASEH1) : CUGUCUUGCUGCCUGUACU. pICE-NLS-mCherry (referred to as pICE-empty in the text; Addgene plasmid, 60364) and pICE-RNase-H1-WT-NLS-mCherry (referred to as pICE-RNase-H1 in the text, Addgene plasmid, 60365) were respectively used as control and for RNase H1 over-expression (gifts from P. Calsou and S. Britton). ..

    Article Title: Chromatin compartmentalization regulates the response to DNA damage.
    Article Snippet: For ATM or DNA-PK inhibition, cells were respectively pretreated for 1 h with 20 μM KU-55933 (Sigma-Aldrich, SML1109) or 2 μM NU-7441 (Selleckchem, S2638) and during subsequent OHT treatment. .. For cell synchronization, cells were incubated for 18 h with 2 mM thymidine (Sigma-Aldrich, T1895), then released during 11 h, followed by a second thymidine treatment for 18 h. S, G2 and G1 cells were then respectively treated with OHT at 0, 6 or 11 h after thymidine release and collected 4 h later. siRNA and plasmid transfections were performed using the 4D-Nucleofector kit and the SE cell line 4D-Nucleofector X L or S kit (Lonza) according to the manufacturer’s instructions, and subsequent treatment(s) were performed 48 h later. siRNA transfections were performed using a control siRNA (siCtrl): CAUGUCAUGUGUCACAUCU; or siRNA targeting SCC1 (siSCC1): GGUGAAAAUGGCAUUACGG; SMC1 (siSMC1): UAGG CUUCCUGGAGGUCACAUUUAA; 53BP1 (si53BP1): GAACGAGGAGA CGGUAAUA; SUN2 (siSUN2): CGAGCCTATTCAGACGTTTCA; ARP2 (siARP2): GGCACCGGGUUUGUGAAGU; SETX (siSETX): GAGAGAA UUAUUGCGUACU; and RNASEH1 (siRNASEH1)48: CUGUCUUGCUGC CUGUACU. pICE-NLS-mCherry (referred to as pICE-empty in the text; Addgene plasmid, 60364) and pICE-RNase-H1-WT-NLS-mCherry (referred to as pICE-RNase-H1 in the text, Addgene plasmid, 60365) were respectively used as control and for RNase H1 over-expression (gifts from P. Calsou and S. Britton). ..

    Control:

    Article Title: Chromatin compartmentalization regulates the response to DNA damage
    Article Snippet: For ATM or DNA-PK inhibition, cells were respectively pretreated for 1 h with 20 μM KU-55933 (Sigma-Aldrich, SML1109) or 2 μM NU-7441 (Selleckchem, S2638) and during subsequent OHT treatment. .. For cell synchronization, cells were incubated for 18 h with 2 mM thymidine (Sigma-Aldrich, T1895), then released during 11 h, followed by a second thymidine treatment for 18 h. S, G2 and G1 cells were then respectively treated with OHT at 0, 6 or 11 h after thymidine release and collected 4 h later. siRNA and plasmid transfections were performed using the 4D-Nucleofector kit and the SE cell line 4D-Nucleofector X L or S kit (Lonza) according to the manufacturer’s instructions, and subsequent treatment(s) were performed 48 h later. siRNA transfections were performed using a control siRNA (siCtrl): CAUGUCAUGUGUCACAUCU; or siRNA targeting SCC1 (siSCC1): GGUGAAAAUGGCAUUACGG; SMC1 (siSMC1): UAGGCUUCCUGGAGGUCACAUUUAA; 53BP1 (si53BP1): GAACGAGGAGACGGUAAUA; SUN2 (siSUN2): CGAGCCTATTCAGACGTTTCA; ARP2 (siARP2): GGCACCGGGUUUGUGAAGU; SETX (siSETX): GAGAGAAUUAUUGCGUACU; and RNASEH1 (siRNASEH1) : CUGUCUUGCUGCCUGUACU. pICE-NLS-mCherry (referred to as pICE-empty in the text; Addgene plasmid, 60364) and pICE-RNase-H1-WT-NLS-mCherry (referred to as pICE-RNase-H1 in the text, Addgene plasmid, 60365) were respectively used as control and for RNase H1 over-expression (gifts from P. Calsou and S. Britton). ..

    Article Title: Chromatin compartmentalization regulates the response to DNA damage.
    Article Snippet: For ATM or DNA-PK inhibition, cells were respectively pretreated for 1 h with 20 μM KU-55933 (Sigma-Aldrich, SML1109) or 2 μM NU-7441 (Selleckchem, S2638) and during subsequent OHT treatment. .. For cell synchronization, cells were incubated for 18 h with 2 mM thymidine (Sigma-Aldrich, T1895), then released during 11 h, followed by a second thymidine treatment for 18 h. S, G2 and G1 cells were then respectively treated with OHT at 0, 6 or 11 h after thymidine release and collected 4 h later. siRNA and plasmid transfections were performed using the 4D-Nucleofector kit and the SE cell line 4D-Nucleofector X L or S kit (Lonza) according to the manufacturer’s instructions, and subsequent treatment(s) were performed 48 h later. siRNA transfections were performed using a control siRNA (siCtrl): CAUGUCAUGUGUCACAUCU; or siRNA targeting SCC1 (siSCC1): GGUGAAAAUGGCAUUACGG; SMC1 (siSMC1): UAGG CUUCCUGGAGGUCACAUUUAA; 53BP1 (si53BP1): GAACGAGGAGA CGGUAAUA; SUN2 (siSUN2): CGAGCCTATTCAGACGTTTCA; ARP2 (siARP2): GGCACCGGGUUUGUGAAGU; SETX (siSETX): GAGAGAA UUAUUGCGUACU; and RNASEH1 (siRNASEH1)48: CUGUCUUGCUGC CUGUACU. pICE-NLS-mCherry (referred to as pICE-empty in the text; Addgene plasmid, 60364) and pICE-RNase-H1-WT-NLS-mCherry (referred to as pICE-RNase-H1 in the text, Addgene plasmid, 60365) were respectively used as control and for RNase H1 over-expression (gifts from P. Calsou and S. Britton). ..



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