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reverse transcriptase buffer (5×) m515a1  (Promega)

 
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    Promega reverse transcriptase buffer (5×) m515a1
    Reverse Transcriptase Buffer (5×) M515a1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5%C3%97+reverse+transcriptase+(rt)+buffer/reverse+transcriptase+buffer++5/pmc04302722-321-45-49
    Average 90 stars, based on 1 article reviews
    reverse transcriptase buffer (5×) m515a1 - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: In vitro transcription and immobilized template analysis of preinitiation complexes
    Article Snippet: Reagents list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 32 P-gamma-ATP (Perkin Elmer BLU002Z500UC) Ammonium Acetate 1 M pH 7 (Ammonium Acetate is a neutral salt) dNTPs (10 mM each) Geiger counter GE BiosciencesTM G50 de-salting column Hybridization Buffer (2×) Isopropyl Alcohol 10× PNK buffer list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 (Supplied with T4 PNK) Reverse Transcriptase list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 (Promega M5108) Reverse Transcriptase Buffer (5×) list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 (Promega M515A1) Reverse transcription primer list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 In this protocol, the primer begins +92 nucleotides downstream of the mRNA transcription start site.

    Article Title: In vitro transcription and immobilized template analysis of preinitiation complexes
    Article Snippet: Reagents 32 P-gamma-ATP (Perkin Elmer BLU002Z500UC) Ammonium Acetate 1 M pH 7 (Ammonium Acetate is a neutral salt) dNTPs (10 mM each) Geiger counter GE BiosciencesTM G50 de-salting column Hybridization Buffer (2×) Isopropyl Alcohol 10× PNK buffer (Supplied with T4 PNK) Reverse Transcriptase (Promega M5108) Reverse Transcriptase Buffer (5×) (Promega M515A1) Reverse transcription primer In this protocol, the primer begins +92 nucleotides downstream of the mRNA transcription start site.

    Article Title: In vitro transcription and immobilized template analysis of preinitiation complexes
    Article Snippet: 32 P-gamma-ATP (Perkin Elmer BLU002Z500UC) Ammonium Acetate 1 M pH 7 (Ammonium Acetate is a neutral salt) dNTPs (10 mM each) Geiger counter GE BiosciencesTM G50 de-salting column Hybridization Buffer (2×) Isopropyl Alcohol 10× PNK buffer (Supplied with T4 PNK) Reverse Transcriptase (Promega M5108) Reverse Transcriptase Buffer (5×) (Promega M515A1) Reverse transcription primer In this protocol, the primer begins +92 nucleotides downstream of the mRNA transcription start site.



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    Journal: iScience

    Article Title: Time course transcriptomic profiling suggests Crp/Fnr transcriptional regulation of nosZ gene in a N 2 O-reducing thermophile

    doi: 10.1016/j.isci.2024.111074

    Figure Lengend Snippet:

    Article Snippet: Maxima H Minus Reverse Transcriptase (200U/μL) with 5×RT Buffer , ThermoFisher , Cat# EP0751.

    Techniques: Virus, Recombinant, Reverse Transcription, Isolation, Control, Sequencing, Mass Spectrometry, Software, In Silico, Targeted Proteomics