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rhodococcus rhodochrous (ATCC)

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ATCC rhodococcus rhodochrous
Growth of rhodococci on seawater-based medium containing the AF of Arabian light crude oil a

Rhodococcus Rhodochrous, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4001/Rhodococcus+rhodochrous/pmc00092421-64-0-3
Average 93 stars, based on 3 article reviews

rhodococcus rhodochrous - by Bioz Stars, 2026-09

93/100 stars

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1) Product Images from "Relationships between Colony Morphotypes and Oil Tolerance in Rhodococcus rhodochrous"

Article Title: Relationships between Colony Morphotypes and Oil Tolerance in Rhodococcus rhodochrous

Journal:

doi:

Figure Legend Snippet: Growth of rhodococci on seawater-based medium containing the AF of Arabian light crude oil a

Techniques Used:

Growth of the colony morphology mutants derived from R. rhodochrous CF222 in SWYAF (A) or in YGAF (B) in the presence or absence of 100 μg of S-2 EPS per ml. The growth temperature was 30°C. (A) Symbols: ●, growth of S-2 in SWYAF; ■, growth of R-1 in SWYAF; ⧫, growth of R-2 in SWYAF; ⊞, growth of R-1 in SWYAF in the presence of S-2 EPS; •, growth of R-2 in SWYAF in the presence of S-2 EPS. (B) Symbols: ○, growth of S-2 in YG; □, growth of R-1 in YG; ▵, growth of R-2 in YG; ●, growth of S-2 in YGAF; ■, growth of R-1 in YGAF; ⧫, growth of R-2 in YGAF; ⊞, growth of R-1 in YGAF in the presence of EPS; •, growth of R-2 in YGAF in the presence of EPS.

Figure Legend Snippet: Growth of the colony morphology mutants derived from R. rhodochrous CF222 in SWYAF (A) or in YGAF (B) in the presence or absence of 100 μg of S-2 EPS per ml. The growth temperature was 30°C. (A) Symbols: ●, growth of S-2 in SWYAF; ■, growth of R-1 in SWYAF; ⧫, growth of R-2 in SWYAF; ⊞, growth of R-1 in SWYAF in the presence of S-2 EPS; •, growth of R-2 in SWYAF in the presence of S-2 EPS. (B) Symbols: ○, growth of S-2 in YG; □, growth of R-1 in YG; ▵, growth of R-2 in YG; ●, growth of S-2 in YGAF; ■, growth of R-1 in YGAF; ⧫, growth of R-2 in YGAF; ⊞, growth of R-1 in YGAF in the presence of EPS; •, growth of R-2 in YGAF in the presence of EPS.

Techniques Used: Derivative Assay

Figure Legend Snippet: Effect of S-2 EPS on the survival of resting cells of Rhodococcus strains treated with 10% (vol/vol) n -hexadecane a

Techniques Used:

In Vitro:

Article Title: Synthetic Cinnamides and Cinnamates: Antimicrobial Activity, Mechanism of Action, and In Silico Study
Article Snippet: .. The compounds were evaluated for in vitro antimicrobial activity against strains of Candida albicans (ATCC-76485), Candida tropicalis (ATCC-13803), Candida glabrata (ATCC-90030), Aspergillus flavus (LM-171), Penicillium citrinum (ATCC-4001), Streptococcus aureus (ATCC-35903), Streptococcus epidermidis (ATCC-12228), and Pseudomonas aeruginosa (ATCC-25853). ..

Article Title: Synthesis and Antimicrobial Evaluation of Chroman-4-One and Homoisoflavonoid Derivatives.
Article Snippet: .. In Vitro Antimicrobial Assay The antimicrobial activity of the compounds was evaluated against strains of S. epidermidis ATCC-12228, P. aeruginosa ATCC-25853, S. enteritidis ATCC-6017, C. albicans ATCC76445, C. albicans LM-92, C. tropicalis ATCC-13803, N. glabratus ATCC-90030, A. flavus ATCC-13013, and P. citrinum ATCC-4001. ..

Activity Assay:

Cell Culture:

Article Title: Integrative Gene Regulatory Network Analysis Discloses Key Driver Genes of Fibromuscular Dysplasia
Article Snippet: .. Immortalized human Bj-5ta fibroblasts (ATCC, CRL-4001) were cultured at 37°C in 5% CO 2 in fibroblast medium. .. Transduction of UBR4 shRNA lentiviral particles and non-silencing Lentiviral shRNA Control (scramble) into Bj-5ta fibroblasts was performed using the GIPZ UBR4 ShRNA Viral particle Starter Kit (Horizon/Dharmacon, VGH5518–200205973, source clone ID: V2LHS 238957) according to the manufacturer’s instructions using MOI=3.

Article Title: L1 retrotransposition is activated by Ten-eleven-translocation protein 1 and repressed by methyl-CpG binding proteins
Article Snippet: .. Human fibroblast cells (BJ-hTERT; ATCC CRL-4001) were cultured in DMEM medium supplemented with 15% FCS. ..

Article Title: Evolution of higher mesenchymal CD44 expression in the human lineage
Article Snippet: .. Human Skin Fibroblasts (BJ-5ta, ATCC CRL-4001) were cultured in a 4:1 mixture of four parts Dulbecco’s Modified Eagle’s Medium containing 4 mM L-glutamine, 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and one part Medium 199 supplemented with 0.01 mg/ml hygromycin B, and 10% fetal bovine serum. .. Human Endometrial Stromal Fibroblasts (T-HESCs, ATCC CRL-4003) were cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s medium and Ham’s F-12 medium with 3.1 g/L glucose and 1 mM sodium pyruvate without phenol red (D2906, Sigma) supplemented with 1.5 g/L sodium bicarbonate, 1% ITS+ Premix (354352, BD), 1% ABAM and 10% Charcoal stripped fetal bovine serum (100–119, Gemini).

Article Title: Effect of photobiomodulation at 830 nm on gene expression correlated with JAK/STAT signalling in wounded and diabetic wounded fibroblasts in vitro.
Article Snippet: .. Commercially purchased human fibroblast cells (BJ-5ta, ATCC® CRL-4001TM) were acquired from the American Type Culture Collection (ATCC) and were cultured according to conventional cell culture methods. .. Cells were cultured in Minimum Essential Medium (MEM) (Sigma-Aldrich, M7278) containing 10% foetal bovine serum (FBS; Sigma-Aldrich, F9665), 1% penicillin–streptomycin (Sigma-Aldrich, P42942), 1% amphotericin B (Sigma-Aldrich, A2942), 0.1 mM nonessential amino acids (NEAA; Sigma-Aldrich, 11 140– 035), 1 mM sodium pyruvate (Sigma-Aldrich, S8636), and 2 mM L-glutamine (Sigma-Aldrich, D6429), and incubated at 37 C (5% CO2; 85% humidity).

Article Title: Human vulnerability to cancer malignancy is enhanced by evolution of higher mesenchymal CD44 expression compared to other mammals
Article Snippet: .. Human Skin Fibroblasts (BJ-5ta, ATCC CRL-4001) were cultured in a 4:1 mixture of 4 parts Dulbecco’s Modified Eagle’s Medium containing 4mM L-glutamine, 4.5g/L glucose and 1.5g/L sodium bicarbonate and 1 part Medium 199 supplemented with 0.01mg/mL hygromycin B, and 10% fetal bovine serum. .. Human Endometrial Stromal Fibroblasts (T-HESCs, ATCC CRL-4003) were cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s medium and Ham’s F-12 medium with 3.1g/L glucose and 1mM sodium pyruvate without phenol red (D2906, Sigma) supplemented with 1.5g/L sodium bicarbonate, 1% ITS+ Premix (354352, BD), 1% ABAM, and 10% Charcoal stripped fetal bovine serum (100-119, Gemini).

Modification:

other:

Article Title: Relationships between Colony Morphotypes and Oil Tolerance in Rhodococcus rhodochrous
Article Snippet: Rhodococcus rhodochrous , ATCC 4001 , − , R.

Image Search Results

( A ) Outline of metabolic inhibitors for screening. ( B ) Human hTERT-immortalized foreskin fibroblast (BJ-5ta, left) and mouse fibroblast (L929, right) cells were treated with inhibitors from A followed by 0.5 μg/mL HT-DNA transfection for 5 hours. Cells were harvested for qPCR analysis of human IFNB or mouse Cxcl10 . ( C ) BJ-5ta cells were treated with 1 mM AOA for 1 hour followed by HT-DNA (0.5 μg/mL) transfection for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( D and E ) L929 ( D ) and MC38 ( E ) were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) or HT-DNA (0.5 μg/mL) stimulation for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( F – H ) Western blot detected phosphorylated (p-) p-IRF3 and p-STAT1 levels in BJ-5ta, L929, and MC38 cells treated as in C – E . ( I ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection or not. Scale bars, 5 μm. ( J ) Schematic of the main targets of AOA. ( K ) Western blot detected p-IRF3, p-STAT1, GOT2, and GPT2 levels in BJ-5ta cells treated as indicated. ( L ) The relative IFNB and CXCL10 mRNA expression in the control BJ-5ta cells versus GOT2 and GPT2 siRNA-silenced BJ-5ta cells after indicated treatment. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by unpaired t test ( C – E , I , and L ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Journal: The Journal of Clinical Investigation

Article Title: Aspartate deficiency amplifies cGAS-STING signaling in antitumor immunity

doi: 10.1172/JCI199716

Figure Lengend Snippet: ( A ) Outline of metabolic inhibitors for screening. ( B ) Human hTERT-immortalized foreskin fibroblast (BJ-5ta, left) and mouse fibroblast (L929, right) cells were treated with inhibitors from A followed by 0.5 μg/mL HT-DNA transfection for 5 hours. Cells were harvested for qPCR analysis of human IFNB or mouse Cxcl10 . ( C ) BJ-5ta cells were treated with 1 mM AOA for 1 hour followed by HT-DNA (0.5 μg/mL) transfection for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( D and E ) L929 ( D ) and MC38 ( E ) were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) or HT-DNA (0.5 μg/mL) stimulation for the indicated time. Cells were harvested for qPCR analysis of IFN response gene expression. ( F – H ) Western blot detected phosphorylated (p-) p-IRF3 and p-STAT1 levels in BJ-5ta, L929, and MC38 cells treated as in C – E . ( I ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection or not. Scale bars, 5 μm. ( J ) Schematic of the main targets of AOA. ( K ) Western blot detected p-IRF3, p-STAT1, GOT2, and GPT2 levels in BJ-5ta cells treated as indicated. ( L ) The relative IFNB and CXCL10 mRNA expression in the control BJ-5ta cells versus GOT2 and GPT2 siRNA-silenced BJ-5ta cells after indicated treatment. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by unpaired t test ( C – E , I , and L ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Six- to 8-week-old C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd, and age-matched Ifnar1 -KO C57BL/6J mice were obtained from Cyagen Biosciences (Suzhou) Inc. BJ-5ta and L929 cells were obtained from ATCC, while B16-F10 and MC38 cells were purchased from the National Collection of Authenticated Cell Cultures.

Techniques: Transfection, Gene Expression, Western Blot, Immunofluorescence, Expressing, Control

( A ) Heatmap of metabolite changes in mock- and AOA-treated L929 cells followed with HT-DNA transfection or without HT-DNA transfection. The fold-change of metabolites abundances was normalized to the mock group. Each square represents the mean of 3 replicates ( n = 3 independent cultures). ( B ) L929 cells were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) stimulation for 6 hours in the absence or presence of 20 mM aspartate or asparagine, and then cells were harvested for qPCR analysis of IFN response gene expression. ( C ) Heatmap from RNA-seq revealing the expression of ISGs in L929 cells with indicated treatment compared with mock group [ z -score–normalized log 2 (fold per million reads) values, n = 3 independent cultures]. ( D ) GSEA of interferon-beta response gene. ( E ) Western blot detected p-IRF3 and p-STAT1 levels in L929 cells treated as indicated. ( F ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection in the absence or presence of 20 mM aspartate. Scale bars, 5 μm. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by 1-way ANOVA followed by Tukey’s test ( B ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Journal: The Journal of Clinical Investigation

Article Title: Aspartate deficiency amplifies cGAS-STING signaling in antitumor immunity

doi: 10.1172/JCI199716

Figure Lengend Snippet: ( A ) Heatmap of metabolite changes in mock- and AOA-treated L929 cells followed with HT-DNA transfection or without HT-DNA transfection. The fold-change of metabolites abundances was normalized to the mock group. Each square represents the mean of 3 replicates ( n = 3 independent cultures). ( B ) L929 cells were treated with 0.5 mM AOA for 1 hour followed by DMXAA (50 μM) stimulation for 6 hours in the absence or presence of 20 mM aspartate or asparagine, and then cells were harvested for qPCR analysis of IFN response gene expression. ( C ) Heatmap from RNA-seq revealing the expression of ISGs in L929 cells with indicated treatment compared with mock group [ z -score–normalized log 2 (fold per million reads) values, n = 3 independent cultures]. ( D ) GSEA of interferon-beta response gene. ( E ) Western blot detected p-IRF3 and p-STAT1 levels in L929 cells treated as indicated. ( F ) Representative immunofluorescence images of IRF3 in BJ-5ta cells treated with AOA 1 hour followed by HT-DNA (0.5 μg/mL) transfection in the absence or presence of 20 mM aspartate. Scale bars, 5 μm. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by 1-way ANOVA followed by Tukey’s test ( B ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Techniques: Transfection, Gene Expression, RNA Sequencing, Expressing, Western Blot, Immunofluorescence

$( A ) Schematic showing that aspartate-mediated pyrimidine nucleotide synthesis is essential for intracellular DNA homeostasis. ( B ) Heatmap from RNA-seq revealing the expression of DNA repair enzymes in L929 cells with indicated treatment. The fold-change of expression was normalized to the mock group ( n = 3 independent cultures). ( C ) Genome browser screenshots of END-seq on mitochondria from L929 cells with indicated treatment. The expanded view of the indicated region shows more detailed genomic features. ( D ) The proportion of END-seq reads in mitochondrial and nuclear chromosomal regions. ( E ) Representative immunofluorescence images of mitochondrial (TOM20, red) or dsDNA (green) in BJ-5ta cells treated with AOA in the absence or presence of 20 mM aspartate for 6 hours. Scale bars, 10 μm. Lower panel: quantification of cytosolic dsDNA in BJ-5ta cells with indicated treatment ( n = 39–51 fields per group from 3 biological replicates). ( F ) Representative immunofluorescence images of BJ-5ta cells treated with ddC (100 μM) for 72 hours, followed by 6 hours of AOA treatment in the absence or presence of 20 mM aspartate. ( G ) 2D–structured illumination microscopy imaging of mtDNA externalization under the indicated conditions. ( H ) Western blot detected α-tubulin (cytosol), TOM20 (OMM), PDH (Matrix), COX IV (IMM), and histone H3 (nuclei) to validate protocol from in L929 cells treated as indicated. wcl, whole cell lysate; pel, pellet; cyt, cytosolic fraction. ( I ) L929 cells were treated with AOA in the absence or presence of 20 mM aspartate followed by HT-DNA transfection, and then cells were harvested for qPCR analysis of mtDNA or nDNA levels in cytosolic fractions. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by 1-way ANOVA followed by Tukey’s test ( E and I ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. DSBs, double-strand breaks.$

Journal: The Journal of Clinical Investigation

Article Title: Aspartate deficiency amplifies cGAS-STING signaling in antitumor immunity

doi: 10.1172/JCI199716

Figure Lengend Snippet: ( A ) Schematic showing that aspartate-mediated pyrimidine nucleotide synthesis is essential for intracellular DNA homeostasis. ( B ) Heatmap from RNA-seq revealing the expression of DNA repair enzymes in L929 cells with indicated treatment. The fold-change of expression was normalized to the mock group ( n = 3 independent cultures). ( C ) Genome browser screenshots of END-seq on mitochondria from L929 cells with indicated treatment. The expanded view of the indicated region shows more detailed genomic features. ( D ) The proportion of END-seq reads in mitochondrial and nuclear chromosomal regions. ( E ) Representative immunofluorescence images of mitochondrial (TOM20, red) or dsDNA (green) in BJ-5ta cells treated with AOA in the absence or presence of 20 mM aspartate for 6 hours. Scale bars, 10 μm. Lower panel: quantification of cytosolic dsDNA in BJ-5ta cells with indicated treatment ( n = 39–51 fields per group from 3 biological replicates). ( F ) Representative immunofluorescence images of BJ-5ta cells treated with ddC (100 μM) for 72 hours, followed by 6 hours of AOA treatment in the absence or presence of 20 mM aspartate. ( G ) 2D–structured illumination microscopy imaging of mtDNA externalization under the indicated conditions. ( H ) Western blot detected α-tubulin (cytosol), TOM20 (OMM), PDH (Matrix), COX IV (IMM), and histone H3 (nuclei) to validate protocol from in L929 cells treated as indicated. wcl, whole cell lysate; pel, pellet; cyt, cytosolic fraction. ( I ) L929 cells were treated with AOA in the absence or presence of 20 mM aspartate followed by HT-DNA transfection, and then cells were harvested for qPCR analysis of mtDNA or nDNA levels in cytosolic fractions. Data are represented as means ± SEM. Representative data are shown from 2 or 3 independent experiments. Statistical analysis was performed by 1-way ANOVA followed by Tukey’s test ( E and I ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. DSBs, double-strand breaks.

Techniques: RNA Sequencing, Expressing, Immunofluorescence, Microscopy, Imaging, Western Blot, Transfection

Review

rhodococcus rhodochrous (ATCC)

Structured Review

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1) Product Images from "Relationships between Colony Morphotypes and Oil Tolerance in Rhodococcus rhodochrous"

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