incubation (Proteintech)

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Proteintech incubation
Incubation, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/14693/NKRF+Antibody/pm41554300-165-10-19
Average 93 stars, based on 10 article reviews

incubation - by Bioz Stars, 2026-09

93/100 stars

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Western Blot:

Article Title: Silencing of lncRNA Gm26917 Attenuates Alveolar Macrophage-mediated Inflammatory Response in LPS-induced Acute Lung Injury Via Inhibiting NKRF Ubiquitination.
Article Snippet: .. The antibodies used for Western blotting, co-immunoprecipitation or immunofluorescence are as follows: NKRF (DF8755,Affinity), GAPDH (T004,Affinity), His-Tag antibody (66,005–1-Ig, Proteintech), Flag-Tag antibody (66,008–4-Ig, Proteintech), IgG(A7028,Beyotime), p65 (YM3111,Immunoway), p-p65 (YP0191, Immunoway), IKBα (10,268–1-AP, Proteintech), p-IKBα (82,349–1- RR, Proteintech), p50 (66,992–1-Ig, Proteintech), F4 / 80 (sc377009,SantaCruz), CD68 (Ab125212,Abcam). .. Data were analyzed using GraphPad Prism 8.0.2 software.

Article Title: Silencing of lncRNA Gm26917 Attenuates Alveolar Macrophage-mediated Inflammatory Response in LPS-induced Acute Lung Injury Via Inhibiting NKRF Ubiquitination
Article Snippet: .. The antibodies used for Western blotting, co-immunoprecipitation or immunofluorescence are as follows: NKRF (DF8755,Affinity), GAPDH (T004,Affinity), His-Tag antibody (66,005–1-Ig, Proteintech), Flag-Tag antibody (66,008–4-Ig, Proteintech), IgG(A7028,Beyotime), p65 (YM3111,Immunoway), p-p65 (YP0191, Immunoway), IKBα (10,268–1-AP, Proteintech), p-IKBα (82,349–1-RR, Proteintech), p50 (66,992–1-Ig, Proteintech), F4 / 80 (sc377009,SantaCruz), CD68 ( Ab125212 ,Abcam). .. Data were analyzed using GraphPad Prism 8.0.2 software.

Article Title: A1CF Binding to the p65 Interaction Site on NKRF Decreased IFN-β Expression and p65 Phosphorylation (Ser536) in Renal Carcinoma Cells
Article Snippet: .. For Western blotting assays, the following antibodies were used: A1CF (1:300, ORIGENE, Inc. Rockville, MD, USA, AP50047PU-N), NKRF (1:2000, Proteintech, Inc. Rosemont, IL, USA, 14693-1-AP), IFN-β (1:300, Proteintech, Inc., 27506-1-AP), p65 (1:1000, Cell Signaling Technology, Inc. Danvers, MA, USA, 6956), p50/105 (1:1000, Cell Signaling Technology, Inc., 13586), p-p65 (Ser536) (1:1000, Cell Signaling Technology, Inc., 3033), IκBα (1:1000, Cell Signaling Technology, Inc. 4814), p-IκBα (Ser32) (1:1000, Cell Signaling Technology, Inc., 2859), NPM1 (1:1000, Proteintech, Inc., 60096-1-Ig), β-actin (1:5000, Sigma-Aldrich, Inc. St. Louis, MO, USA, A1978), and GAPDH (1:5000, Sigma-Aldrich, Inc., SAB2701825). .. For Immunofluorescence and immunohistochemistry, the following antibodies were used: A1CF (1:50, ORIGENE, Inc., AP50047PU-N), NKRF (1:200, Santa Cruz Biotechnology, Inc. Santa Cruz, CA, USA, sc-365568), IFN-β (1:50, Proteintech, Inc., 27506-1-AP), p65 (1:200, Cell Signaling Technology, Inc., 6956), and p-p65(S536) (1:200, Cell Signaling Technology, Inc., 3033).

Immunofluorescence:

Article Title: Silencing of lncRNA Gm26917 Attenuates Alveolar Macrophage-mediated Inflammatory Response in LPS-induced Acute Lung Injury Via Inhibiting NKRF Ubiquitination
Article Snippet: .. The antibodies used for Western blotting, co-immunoprecipitation or immunofluorescence are as follows: NKRF (DF8755,Affinity), GAPDH (T004,Affinity), His-Tag antibody (66,005–1-Ig, Proteintech), Flag-Tag antibody (66,008–4-Ig, Proteintech), IgG(A7028,Beyotime), p65 (YM3111,Immunoway), p-p65 (YP0191, Immunoway), IKBα (10,268–1-AP, Proteintech), p-IKBα (82,349–1-RR, Proteintech), p50 (66,992–1-Ig, Proteintech), F4 / 80 (sc377009,SantaCruz), CD68 ( Ab125212 ,Abcam). .. Data were analyzed using GraphPad Prism 8.0.2 software.

Incubation:

Article Title: E3 Ubiquitination Ligase MYLIP Mediates the NKRF/SLC25A34 Axis to Suppress Malignant Progression in Colorectal Cancer.
Article Snippet: Background The SLC25 family of transport proteins has been implicated in colorectal cancer (CRC) tumorigenesis.. However, the potential role of SLC25A34 in CRC remains unclear.. This study was designed to elucidate the role and mechanism of SLC25A34 in CRC.

Article Title: Treadmill Exercise Attenuates CUMS-Induced Depressive Behaviors by Modulating the UPRmt via the Nrf2/Keap1 Pathway.
Article Snippet: .. Following a 1-hour block at room temperature, sections were incubated overnight (4 °C) with primary antibodies against Nrf2 (14693-1-AP, 1:200, Proteintech) or HSP60 (ab190828, 1:200, Abcam). ..

Article Title: Treadmill Exercise Attenuates CUMS-Induced Depressive Behaviors by Modulating the UPRmt via the Nrf2/Keap1 Pathway.
Article Snippet: .. The membranes were then blocked (1 h, RT) before overnight incubation (4 °C) with primary antibodies: Nrf2 (14693-1-AP, 1:1,000, Proteintech), Keap1 (10503-2-AP, 1:1,000, Proteintech), NQO1 (ab80588, 1:1,000, Abcam), HO-1 (ab189491, 1:1,000, Abcam), HSP60 (ab190828, 1:1,000, Abcam), ClpP (ab124822, 1:1,000, Abcam), HSP70 (ab194360, 1:1,500, Abcam), LONP1 (15440-1-AP, 1:1,000, Proteintech), ATF5 (MA563234, 1:1,000, Thermo Fisher), CHOP (15204-1-AP, 1:1,000, Proteintech), and β-actin (ab179467, 1:5,000, Abcam). .. Subsequently, membranes were incubated with an HRP-conjugated secondary antibody (ab6721, 1:10,000, Abcam) for 1 h at RT, washed (3 × 10 min, TBST), and visualized via an ECL system.

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