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fluorescence  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec fluorescence
    Fluorescence, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/130-109-803/MACSQuant+Analyzer+16/pmc13373552-73-2-10
    Average 96 stars, based on 231 article reviews
    fluorescence - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: Age-associated niche remodeling drives dental pulp progenitor dysfunction and fibrosis.
    Article Snippet: Antibodies used for flow cytometry were CD45-PE (1:100, Invitrogen, MA5-17964), CD68-APC (1:100, Invitrogen, MA5-23616), CCL4 (Rabbit, 1:50, Invitrogen, 710391), FITC-conjugated Goat Anti-Rabbit IgG (SA00003-2, Proteintech, 1:300). .. Data acquisition was performed using a MACSQuant Analyzer 16 flow cytometer (Miltenyi Biotec), and data were analyzed using FlowJo software. .. Second-passage human dental pulp cells were dissociated using 0.25% trypsin, counted, and incubated with Fc receptor blocking reagent (Miltenyi Biotec, 130-092-575) prior to primary and secondary antibodies incubation according to the manufacture’s instruction.

    Article Title: Rapid and robust immune response boosting with potent, next-generation adjuvant in viral vector-primed primates.
    Article Snippet: Cells were permeabilized with Caltag Fixation and Permeabilization Kit (Life Technologies) and stained intracellularly with IL-2 PE (BD Biosciences), IFNγ PE Cy7 (BD Biosciences), CD69 Texas Red (BD Biosciences), and TNFα-FITC (BD Biosciences). .. Fluorescence was measured by flow cytometry on a Fortessa (BD Biosciences) or the MACSQuant Analyzer 16 (Miltenyi Biotec) (Fig. S15). .. Binding of antibodies and plasma to cell surface-expressed Env 293T human embryonic kidney cells (ATCC) and CD4+ T lymphocytes were isolated, activated, and cultured as previously described (111).

    Article Title: Radioligand therapy in combination with CAR T cells overcomes the heterogeneous immunosuppressive prostate tumor microenvironment
    Article Snippet: .. Flow cytometry was performed using a MACSQuant Analyzer 16 (Miltenyi Biotec), and data were analyzed using FlowJo software (FlowJo LLC, v10.10). .. Male NSG mice (5-6 weeks old; Jackson Laboratory) were housed in a pathogen-free facility at the University of California Los Angeles (UCLA) under a 12-hour light/dark cycle with ad libitum access to food and water.

    Article Title: Antioxidant and Geno-protective Effects of Naturally Occurring Delphinidin Glycosides.
    Article Snippet: Anthocyanins are flavonoid pigments responsible for red, blue, and purple coloration in plants and play a protective role against abiotic stress through reactive oxygen species scavenging.. They are abundant in the human diet, with berries serving as a primary source.. These flavonoids are known for their antioxidant and potential geno-protective properties.

    Article Title: Focal radiotherapy improves CAR T cell therapy targeting prostate cancer
    Article Snippet: Cell viability was determined using 4’, 6-diamidino-2-phenylindole (DAPI, Sigma, Cat: D8417). .. Flow cytometry was performed using the MACSQuant Analyzer 10 or the MACSQuant Analyzer 16 (Miltenyi Biotec). .. Data analysis was performed using FlowJo software (FlowJo LLC, v10.10).

    Article Title: Erastin-Induced Ferroptosis Overcomes Oxaliplatin Resistance in Colorectal Cancer Cells.
    Article Snippet: .. Fluorescence was analyzed using a MACSQuant Analyzer 16 flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany) with the B1 channel (525/50 nm). ..

    Software:

    Article Title: Age-associated niche remodeling drives dental pulp progenitor dysfunction and fibrosis.
    Article Snippet: Antibodies used for flow cytometry were CD45-PE (1:100, Invitrogen, MA5-17964), CD68-APC (1:100, Invitrogen, MA5-23616), CCL4 (Rabbit, 1:50, Invitrogen, 710391), FITC-conjugated Goat Anti-Rabbit IgG (SA00003-2, Proteintech, 1:300). .. Data acquisition was performed using a MACSQuant Analyzer 16 flow cytometer (Miltenyi Biotec), and data were analyzed using FlowJo software. .. Second-passage human dental pulp cells were dissociated using 0.25% trypsin, counted, and incubated with Fc receptor blocking reagent (Miltenyi Biotec, 130-092-575) prior to primary and secondary antibodies incubation according to the manufacture’s instruction.

    Article Title: Radioligand therapy in combination with CAR T cells overcomes the heterogeneous immunosuppressive prostate tumor microenvironment
    Article Snippet: .. Flow cytometry was performed using a MACSQuant Analyzer 16 (Miltenyi Biotec), and data were analyzed using FlowJo software (FlowJo LLC, v10.10). .. Male NSG mice (5-6 weeks old; Jackson Laboratory) were housed in a pathogen-free facility at the University of California Los Angeles (UCLA) under a 12-hour light/dark cycle with ad libitum access to food and water.

    Fluorescence:

    Article Title: Rapid and robust immune response boosting with potent, next-generation adjuvant in viral vector-primed primates.
    Article Snippet: Cells were permeabilized with Caltag Fixation and Permeabilization Kit (Life Technologies) and stained intracellularly with IL-2 PE (BD Biosciences), IFNγ PE Cy7 (BD Biosciences), CD69 Texas Red (BD Biosciences), and TNFα-FITC (BD Biosciences). .. Fluorescence was measured by flow cytometry on a Fortessa (BD Biosciences) or the MACSQuant Analyzer 16 (Miltenyi Biotec) (Fig. S15). .. Binding of antibodies and plasma to cell surface-expressed Env 293T human embryonic kidney cells (ATCC) and CD4+ T lymphocytes were isolated, activated, and cultured as previously described (111).

    Article Title: Antioxidant and Geno-protective Effects of Naturally Occurring Delphinidin Glycosides.
    Article Snippet: Anthocyanins are flavonoid pigments responsible for red, blue, and purple coloration in plants and play a protective role against abiotic stress through reactive oxygen species scavenging.. They are abundant in the human diet, with berries serving as a primary source.. These flavonoids are known for their antioxidant and potential geno-protective properties.

    Article Title: Erastin-Induced Ferroptosis Overcomes Oxaliplatin Resistance in Colorectal Cancer Cells.
    Article Snippet: .. Fluorescence was analyzed using a MACSQuant Analyzer 16 flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany) with the B1 channel (525/50 nm). ..

    Staining:

    Article Title: WT1-Targeted Oral Bifidobacterium longum Vaccine Enhances Checkpoint Blockade Efficacy in Pancreatic Cancer.
    Article Snippet: Cells were washed and stained with Brilliant Violet 605-anti-mouse IFN- γ, PE-Cy7-anti-mouse TNF- α, APC-antimouse IL-12, PE-Dazzle-594-anti mouse Granzyme B or PEanti-mouse IL-2 (BioLegend) for intracellular staining. .. Stained cells were analyzed using a MACSQuant Analyzer 16 (Miltenyi Biotec). .. After 6 h of culture, GolgiStop (BD Biosciences, San Jose, CA, USA) was added to the culture, and the cells were then cultured for an additional 12 h. For intracellular cytokine staining (ICCS), cells were collected and processed using a BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit (BD Biosciences) according to our previous study [ 21 ].

    other:

    Article Title: Targeting ferroptosis in gefitinib-resistant HCC827 NSCLC cells using erastin
    Article Snippet: After washing, fluorescence was acquired using a MACSQuant Analyzer 16 (Miltenyi Biotec) at 543/580 nm.



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