homogenizing ball (Valiant Co Ltd)

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Valiant Co Ltd homogenizing ball
Homogenizing Ball, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/04822761/RIPA+buffer/pm30234992-122-19-21
Average 96 stars, based on 203 article reviews

homogenizing ball - by Bioz Stars, 2026-09

96/100 stars

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Protease Inhibitor:

Article Title: Myeloid‐Derived CD38 Mediates Age‐Related Endometrial Aging Through NAD + Depletion
Article Snippet: .. We added cold RIPA buffer containing a protease inhibitor (Sigma‐Aldrich) to the tissues and homogenized the tissues using a FastPrep‐24 5G (MP Biomedicals). ..

Article Title: Procyanidin A2, a polyphenol autophagy-enhancer, ameliorates hyperglycemia through multifaceted insulin-related mechanisms
Article Snippet: The purity of PCA2 (95%–99%) was analyzed using HPLC, with its identification confirmed by MS and NMR. α-Glucosidase (G5003, Sigma-Aldrich, St. Louis, MO, USA), p-nitrophenyl-α-D-glucopyranoside (pNPG, N1377, Sigma-Aldrich), Acarbose (A8980, Sigma-Aldrich), colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, M2128, Sigma-Aldrich), and 2’,7’-dichlorofluorescein-diacetate (DCFH-DA; 35845-5G, Sigma-Aldrich) were also used. .. RIPA buffer (EBA-1149, Elpis Biotech, Daejeon, Korea), protease inhibitor cocktail I (P8340, Sigma-Aldrich), phosphatase inhibitor cocktail II (P5726, Sigma-Aldrich), skim milk (MB-S16676, MB Cell, Seoul, Korea), and bovine serum albumin (BSA, 160069, MP Biomedicals., LLC, Illkirch-Graffenstaden, France) were obtained commercially. .. Additionally, GLUT4, p-IRS1, AKT, PI3K, p-AMPK, and AMPK (Bioworld Technology, Inc., MN, USA); IRS1, p-AKT, PDK1, GSK3β, p-GSK3β, HO-1, LC3B, Atg5, Beclin1, p62, and α-tubulin (Cell Signaling Technology Inc., Beverly, MA, USA); p-PI3K, Nrf2 (Abcam, Cambridge, UK); β-actin, GPx-1, SOD1, and CAT (Santa Cruz Technology, Inc., Heidelberg, Germany); and anti-mouse, anti-rabbit, and anti-goat IgG HRP-linked antibodies (Cell Signaling Technology Inc., Beverly, MA, USA) were all commercially available and used in the study.

Article Title: The monkeypox virus suppresses autophagy by modulating Rubicon expression.
Article Snippet: The primary antibodies used in this study were rabbit anti-LC3 (Cell Signaling Technology, 2775), mouse anti SQSTM1/p62 (MBL, PM045), rabbit anti L1R (Sino Biological, 40889-T62), mouse anti Actin (Santa Cruz Biotecnology, sc-47778), mouse anti Tubulin (Santa Cruz Biotecnology, sc-32293), rabbit anti Rubicon (Cell Signaling Technology, 8465), mouse anti HSP90 (Santa Cruz Biotecnology, sc-13119), rabbit anti Ambra1 (Millipore, ABC131), mouse anti Beclin1 (Santa Cruz, E-8, sc-48341), rabbit anti ULK1 (Cell Signaling Technology, 8054), rabbit anti UVRAG (Sigma-Aldrich, U7508), rabbit anti Optineurin (Abcam, ab23666), rabbit anti NDP52 (Proteintech 12229-1-AP) ( rabbit anti p21 (Cell Signaling Technology, 2947), rabbit anti p53 (Santa Cruz Biotecnology, sc-126), rabbit anti ATG7 (Abcam, ab133528), rabbit Anti-Ubiquitin Lys48-Specific (Millipore, 05-1307), mouse anti GAPDH (Calbiochem, CB1001). .. Cells were lysed in RIPA buffer: 150 mM NaCl (Sigma-Aldrich, S7653), 1% NP-40 (SigmaAldrich, 56741), 0.5% deoxycholic acid (MP Biomedicals, 101496), 0.1% SDS (Sigma-Aldrich, L3771), 50 mM tris (pH 8.0) (Santa Cruz Biotechnology, sc-3715A), and 2 mM MgCl2 (SigmaAldrich, M8266), with protease and phosphatase inhibitors: Protease Inhibitor Cocktail plus (Sigma-Aldrich, P8340), 5 mM sodium fluoride (Sigma-Aldrich, S-7920), 0.5 mM sodium orthovanadate (Sigma-Aldrich, S6508), 1 mM sodium molybdate (Sigma-Aldrich, S-6646), 50 mM 2-chloroacetamide (Sigma-Aldrich, C0267), 2 mM 1,10-phenanthroline monohydrate (Sigma-Aldrich, 320056), and 0.5 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich, P7626). .. AR TI CL E IN P RE SS Protein total extracts were separated on SDS PAGE gels and electroblotted onto nitrocellulose (Whatman Amersham, 10600041) or PVDF (Millipore, IPVH20200) membranes.

Article Title: The monkeypox virus suppresses autophagy by modulating Rubicon expression
Article Snippet: The primary antibodies used in this study were rabbit anti-LC3 (Cell Signaling Technology, 2775), mouse anti SQSTM1/p62 (MBL, PM045), rabbit anti-L1R (Sino Biological, 40889-T62), mouse anti-Actin (Santa Cruz Biotecnology, sc-47778), mouse anti Tubulin (Santa Cruz Biotecnology, sc-32293), rabbit anti-Rubicon (Cell Signaling Technology, 8465), mouse anti-HSP90 (Santa Cruz Biotecnology, sc-13119), rabbit anti-Ambra1 (Millipore, ABC131), mouse anti Beclin1 (Santa Cruz, E-8, sc-48341), rabbit anti-ULK1 (Cell Signaling Technology, 8054), rabbit anti-UVRAG (Sigma-Aldrich, U7508), rabbit anti-Optineurin (Abcam, ab23666), rabbit anti NDP52 (Proteintech 12229-1-AP) (rabbit anti-p21 (Cell Signaling Technology, 2947), rabbit anti-p53 (Santa Cruz Biotecnology, sc-126), rabbit anti-ATG7 (Abcam, ab133528), rabbit anti-ubiquitin Lys48-specific (Millipore, 05-1307), mouse anti-GAPDH (Calbiochem, CB1001). .. Cells were lysed in RIPA buffer: 150 mM NaCl (Sigma-Aldrich, S7653), 1% NP-40 (Sigma-Aldrich, 56741), 0.5% deoxycholic acid (MP Biomedicals, 101496), 0.1% SDS (Sigma-Aldrich, L3771), 50 mM tris (pH 8.0) (Santa Cruz Biotechnology, sc-3715A), and 2 mM MgCl 2 (Sigma-Aldrich, M8266), with protease and phosphatase inhibitors: Protease Inhibitor Cocktail plus (Sigma-Aldrich, P8340), 5 mM sodium fluoride (Sigma-Aldrich, S-7920), 0.5 mM sodium orthovanadate (Sigma-Aldrich, S6508), 1 mM sodium molybdate (Sigma-Aldrich, S-6646), 50 mM 2-chloroacetamide (Sigma-Aldrich, C0267), 2 mM 1,10-phenanthroline monohydrate (Sigma-Aldrich, 320056), and 0.5 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich, P7626). .. Protein total extracts were separated on SDS–PAGE gels and electroblotted onto nitrocellulose (Whatman Amersham, 10600041) or PVDF (Millipore, IPVH20200) membranes.

Article Title: Enhanced ISGylation via USP18 Isopeptidase Inactivation Fails to Mitigate the Inflammatory or Functional Course of Coxsackievirus B3-Induced Myocarditis.
Article Snippet: The reactions were performed on the StepOnePlus real-time PCR system (Thermo Fisher Scientific), with gene expression normalized to the housekeeping gene Hprt and calculated as ΔCt values. .. Protein Quantification and Immunoblotting Snap-frozen heart tissue was added to Lysis Matrix D tubes with RIPA-buffer with 1, 25X Protease Inhibitor Cocktail (Roche) and tissue was homogenized 3 times for 30 seconds using the MP Biomedical FastPrep, centrifuged and the supernatant was used for further analysis. .. Protein concentration was determined using the Bradford assay (Thermo Fisher Scientific) following the manufacturer’s instructions.

Article Title: Myeloid-Derived CD38 Mediates Age-Related Endometrial Aging Through NAD + Depletion.
Article Snippet: .. We added cold RIPA buffer containing a protease inhibitor (Sigma- Aldrich) to the tissues and homogenized the tissues using a FastPrep- 24 5G (MP Biomedicals). ..

Article Title: Procyanidin A2, a polyphenol autophagy-enhancer, ameliorates hyperglycemia through multifaceted insulin-related mechanisms.
Article Snippet: The purity of PCA2 (95%–99%) was analyzed using HPLC, with its identification confirmed by MS and NMR. α-Glucosidase (G5003, Sigma-Aldrich, St. Louis, MO, USA), p-nitrophenyl-α-D-glucopyranoside (pNPG, N1377, Sigma-Aldrich), Acarbose (A8980, Sigma-Aldrich), colorimetric 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl-2H-tetrazolium bromide (MTT, M2128, Sigma-Aldrich), and 2’,7’-dichlorofluorescein-diacetate (DCFH-DA; 35845-5G, Sigma-Aldrich) were also used. .. RIPA buffer (EBA-1149, Elpis Biotech, Daejeon, Korea), protease inhibitor cocktail I (P8340, Sigma-Aldrich), phosphatase inhibitor cocktail II (P5726, Sigma-Aldrich), skim milk (MB-S16676, MB Cell, Seoul, Korea), and bovine serum albumin (BSA, 160069, MP Biomedicals., LLC, Illkirch-Graffenstaden, France) were obtained commercially. .. Additionally, GLUT4, p-IRS1, AKT, PI3K, p-AMPK, and AMPK (Bioworld Technology, Inc., MN, USA); IRS1, p-AKT, PDK1, GSK3β, p-GSK3β, HO1, LC3B, Atg5, Beclin1, p62, and α-tubulin (Cell Signaling Technology Inc., Beverly, MA, USA); p-PI3K, Nrf2 (Abcam, Cambridge, UK); β-actin, GPx-1, SOD1, and CAT (Santa Cruz Technology, Inc., Heidelberg, Germany); and anti-mouse, anti-rabbit, and anti-goat IgG HRP-linked antibodies (Cell Signaling Technology Scientific Reports | (2025) 15:41248 2| https://doi.org/10.1038/s41598-025-25111-3 Inc., Beverly, MA, USA) were all commercially available and used in the study.

Radio Immunoprecipitation:

Article Title: Integrative analysis of inhibition of advanced glycation end-products accumulation and prevention of aging muscle deterioration in animals and human models.
Article Snippet: .. GCM tissues were homogenized in radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors using a FastPrep24 homogenizer (MP Biomedicals, Solon, OH, USA). ..

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