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Valiant Co Ltd smai dephosphorylated puc19 plasmid vector
Smai Dephosphorylated Puc19 Plasmid Vector, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/04821227/pUC19+plasmid/10__1007_slash_s13258___010___0091___9-58-11-16
Average 90 stars, based on 28 article reviews
smai dephosphorylated puc19 plasmid vector - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

BAC Assay:

Article Title: Polymorphisms of the 5' regulatory region of the porcine PPARGC1A gene and the effects on muscle fiber characteristics and meat quality.
Article Snippet: The purpose of this study was to determine the structure of the porcine PPARGC1A 50 upstream region, and to find suitable molecular markers for improved meat quality and good lean meat production.. Ten DNA polymorphisms, including 7 SNPs, 2 microsatellites, and 1 insertion or deletion were newly found in the 50 upstream region of PPARGC1A.. Three SNPs that had restriction enzyme site were evaluated for associations with muscle fiber characteristics and production traits.

Article Title: Structure and polymorphisms of the 5′ regulatory region of porcine adenylate kinase 3-like 1 gene and effect on trait of meat quality
Article Snippet: The porcine adenylate kinase 3-like 1 (AK3L1) gene is considered as a potential candidate gene among the encoded isoforms of adenylate kinase for meat quality based on the functional role of the AK3L1 gene in energy metabolism via high-energy phosphoryl transfer reaction.. The genomic position of the AK3L1 gene is located in the QTL region, which codes for traits of meat quality.. We characterized the genomic structure of the porcine AK3L1 gene and found polymorphisms in the 5’ regulatory region.

Construct:

Article Title: Polymorphisms of the 5' regulatory region of the porcine PPARGC1A gene and the effects on muscle fiber characteristics and meat quality.
Article Snippet: The purpose of this study was to determine the structure of the porcine PPARGC1A 50 upstream region, and to find suitable molecular markers for improved meat quality and good lean meat production.. Ten DNA polymorphisms, including 7 SNPs, 2 microsatellites, and 1 insertion or deletion were newly found in the 50 upstream region of PPARGC1A.. Three SNPs that had restriction enzyme site were evaluated for associations with muscle fiber characteristics and production traits.

Article Title: Structure and polymorphisms of the 5′ regulatory region of porcine adenylate kinase 3-like 1 gene and effect on trait of meat quality
Article Snippet: The porcine adenylate kinase 3-like 1 (AK3L1) gene is considered as a potential candidate gene among the encoded isoforms of adenylate kinase for meat quality based on the functional role of the AK3L1 gene in energy metabolism via high-energy phosphoryl transfer reaction.. The genomic position of the AK3L1 gene is located in the QTL region, which codes for traits of meat quality.. We characterized the genomic structure of the porcine AK3L1 gene and found polymorphisms in the 5’ regulatory region.

Plasmid Preparation:

Article Title: Polymorphisms of the 5' regulatory region of the porcine PPARGC1A gene and the effects on muscle fiber characteristics and meat quality.
Article Snippet: The purpose of this study was to determine the structure of the porcine PPARGC1A 50 upstream region, and to find suitable molecular markers for improved meat quality and good lean meat production.. Ten DNA polymorphisms, including 7 SNPs, 2 microsatellites, and 1 insertion or deletion were newly found in the 50 upstream region of PPARGC1A.. Three SNPs that had restriction enzyme site were evaluated for associations with muscle fiber characteristics and production traits.

Article Title: Structure and polymorphisms of the 5′ regulatory region of porcine adenylate kinase 3-like 1 gene and effect on trait of meat quality
Article Snippet: The porcine adenylate kinase 3-like 1 (AK3L1) gene is considered as a potential candidate gene among the encoded isoforms of adenylate kinase for meat quality based on the functional role of the AK3L1 gene in energy metabolism via high-energy phosphoryl transfer reaction.. The genomic position of the AK3L1 gene is located in the QTL region, which codes for traits of meat quality.. We characterized the genomic structure of the porcine AK3L1 gene and found polymorphisms in the 5’ regulatory region.

Article Title: Ten polymorphic microsatellite loci for the trematode Curtuteria australis (Echinostomatidae).
Article Snippet: Ten polymorphic loci were isolated and characterised from the intertidal New Zealand trematode Curtuteria australis.. This common parasite manipulates the burrowing behaviour of its abundant bivalve host Austrovenus stutchburyi, with cascading impacts on the biodiversity of intertidal communities.. Observed heterozygosities of the 10 loci ranged from 0.500 to 0.905, and three to 14 alleles were detected in 24 trematode metacercariae.

Article Title: MICROSATELLITE GENOTYPING OF SINGLE CELLS OF THE DINOFLAGELLATE SPECIES LINGULODINIUM POLYEDRUM (DINOPHYCEAE): A NOVEL APPROACH FOR MARINE MICROBIAL POPULATION GENETIC STUDIES(1).
Article Snippet: In recent years, two new approaches have been introduced in genetic studies of phytoplankton species.. One is the application of highly polymorphic microsatellite markers, which allow detailed population genetic studies; the other is the development of methods that enable the direct genetic characterization of single cells as an alternative to clonal cultures.. The aim of this study was to combine these two approaches in a method that would allow microsatellite genotyping of single phytoplankton cells, providing a novel tool for high-resolution population genetic studies.

Article Title: [2009.10378] Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities
Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation according to the method of Dennis and Sokol (8). .. For the construction of the pUC19-derived plasmid dedicated to promoter exchange by homologous recombination in B. subtilis, the pbp and fenF fragments were generated by PCR using Taq polymerase “Arrow” from Qbiogene (Montreal, Canada). .. The primers were designed according to the published sequence of the mycosubtilin operon from strain ATCC 6633 (PubMed Nucleotide AF184956) (9).

Article Title: Microsatellite loci for Euglossa annectans (Hymenoptera: Apidae) and their variability in other orchid bees.
Article Snippet: Orchid or euglossine bees are conspicuous Hymenoptera of the Neotropics, where they pollinate numerous plants, including orchids.. Allozyme-based analyses have suggested that their populations suffer from inbreeding, as evidenced by so-called diploid male production.. We have developed nine polymorphic microsatellite loci for the widespread Euglossa annectans, with observed heterozygosities ranging from 0.143 to 0.952 and between 2 and 9 alleles per species.

Article Title: Characterization of 14 polymorphic microsatellite loci for the facultatively eusocial sweat bee Halictus rubicundus (Hymenoptera, Halictidae) and their variability in related species.
Article Snippet: Sweat bees display considerable variation in social organization and a few species, such as Halictus rubicundus, are even facultatively eusocial.. Fourteen polymorphic, unlinked microsatellite loci were isolated from H. rubicundus and characterized in 45 females.. The number of alleles per locus ranged from two to 18 (mean 10.1), observed heterozygosity ranged from 0.24 to 0.98 (mean 0.71) and expected heterozygosity ranged from 0.24 to 0.98 (mean 0.70).

Sequencing:

Article Title: Polymorphisms of the 5' regulatory region of the porcine PPARGC1A gene and the effects on muscle fiber characteristics and meat quality.
Article Snippet: The purpose of this study was to determine the structure of the porcine PPARGC1A 50 upstream region, and to find suitable molecular markers for improved meat quality and good lean meat production.. Ten DNA polymorphisms, including 7 SNPs, 2 microsatellites, and 1 insertion or deletion were newly found in the 50 upstream region of PPARGC1A.. Three SNPs that had restriction enzyme site were evaluated for associations with muscle fiber characteristics and production traits.

Article Title: Structure and polymorphisms of the 5′ regulatory region of porcine adenylate kinase 3-like 1 gene and effect on trait of meat quality
Article Snippet: The porcine adenylate kinase 3-like 1 (AK3L1) gene is considered as a potential candidate gene among the encoded isoforms of adenylate kinase for meat quality based on the functional role of the AK3L1 gene in energy metabolism via high-energy phosphoryl transfer reaction.. The genomic position of the AK3L1 gene is located in the QTL region, which codes for traits of meat quality.. We characterized the genomic structure of the porcine AK3L1 gene and found polymorphisms in the 5’ regulatory region.

Cloning:

Article Title: Ten polymorphic microsatellite loci for the trematode Curtuteria australis (Echinostomatidae).
Article Snippet: Ten polymorphic loci were isolated and characterised from the intertidal New Zealand trematode Curtuteria australis.. This common parasite manipulates the burrowing behaviour of its abundant bivalve host Austrovenus stutchburyi, with cascading impacts on the biodiversity of intertidal communities.. Observed heterozygosities of the 10 loci ranged from 0.500 to 0.905, and three to 14 alleles were detected in 24 trematode metacercariae.

Transformation Assay:

Article Title: MICROSATELLITE GENOTYPING OF SINGLE CELLS OF THE DINOFLAGELLATE SPECIES LINGULODINIUM POLYEDRUM (DINOPHYCEAE): A NOVEL APPROACH FOR MARINE MICROBIAL POPULATION GENETIC STUDIES(1).
Article Snippet: In recent years, two new approaches have been introduced in genetic studies of phytoplankton species.. One is the application of highly polymorphic microsatellite markers, which allow detailed population genetic studies; the other is the development of methods that enable the direct genetic characterization of single cells as an alternative to clonal cultures.. The aim of this study was to combine these two approaches in a method that would allow microsatellite genotyping of single phytoplankton cells, providing a novel tool for high-resolution population genetic studies.

Article Title: Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
Article Snippet: This study was conducted to isolate and functionally characterize a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens.. A novel gene, KG42, was isolated from one of the 17 xylandegrading metagenomic fosmid clones obtained from black goat rumens.. The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a “salad-bowl” shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.

Homologous Recombination:

Article Title: [2009.10378] Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities
Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation according to the method of Dennis and Sokol (8). .. For the construction of the pUC19-derived plasmid dedicated to promoter exchange by homologous recombination in B. subtilis, the pbp and fenF fragments were generated by PCR using Taq polymerase “Arrow” from Qbiogene (Montreal, Canada). .. The primers were designed according to the published sequence of the mycosubtilin operon from strain ATCC 6633 (PubMed Nucleotide AF184956) (9).

Generated:

Article Title: [2009.10378] Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities
Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation according to the method of Dennis and Sokol (8). .. For the construction of the pUC19-derived plasmid dedicated to promoter exchange by homologous recombination in B. subtilis, the pbp and fenF fragments were generated by PCR using Taq polymerase “Arrow” from Qbiogene (Montreal, Canada). .. The primers were designed according to the published sequence of the mycosubtilin operon from strain ATCC 6633 (PubMed Nucleotide AF184956) (9).

Polymerase Chain Reaction:

Article Title: [2009.10378] Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities
Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation according to the method of Dennis and Sokol (8). .. For the construction of the pUC19-derived plasmid dedicated to promoter exchange by homologous recombination in B. subtilis, the pbp and fenF fragments were generated by PCR using Taq polymerase “Arrow” from Qbiogene (Montreal, Canada). .. The primers were designed according to the published sequence of the mycosubtilin operon from strain ATCC 6633 (PubMed Nucleotide AF184956) (9).

Electroporation:

Article Title: Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library.
Article Snippet: This study was conducted to isolate and functionally characterize a novel xylan-degrading enzyme from the microbial metagenomes of black goat rumens.. A novel gene, KG42, was isolated from one of the 17 xylandegrading metagenomic fosmid clones obtained from black goat rumens.. The KG42 gene, comprising a 1107 bp open reading frame, encodes a protein composed of 368 amino acids (41 kDa) with a glycosyl hydrolase family 10 (GH10) domain, consisting of a “salad-bowl” shaped tertiary structure (a typical 8-fold α/β barrel (α/β)8) and two catalytic residues.



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