Lysis:Article Title: Characterization of the gastrointestinal microbiome of the Syrian hamster ( Mesocricetus auratus ) and comparison to data from mice
Article Snippet: .. Samples were thawed on ice, and microbial cell walls were lysed using both a chemical lysis step with 4 m guanidine‐thiocyanate and 5% N ‐laurolylsarcosine and a mechanical lysis step conducted in Lysing Matrix Tubes B (MP Biomedicals, Eschwege, Germany) with the FastPrep 24TM (MP Biomedicals). .. Thereafter, DNA was cleaned up using the NuceloSpin gDNA cleanup kit according to the manufacturer's instructions (Macherey Nagel GmbH and Co. KG, Düren, Germany).
Article Title: Characterization of the gastrointestinal microbiome of the Syrian hamster (Mesocricetus auratus) and comparison to data from mice.
Article Snippet: .. Samples were thawed on ice, and microbial cell walls were lysed using both a chemical lysis step with 4 M guanidine-thiocyanate and 5% N-laurolylsarcosine and a mechanical lysis step conducted in Lysing Matrix Tubes B (MP Biomedicals, Eschwege, Germany) with the FastPrep 24TM (MP Biomedicals). .. Thereafter, DNA was cleaned up using the NuceloSpin gDNA cleanup kit according to the manufacturer’s instructions (Macherey Nagel GmbH and Co. KG, D€uren, Germany).
Tissue Homogenization:Article Title: Vitamin D enrichment of eggs and its effects on performance and intestinal health in late-stage laying hens
Article Snippet: Total RNA was extracted using the RNeasy Fibrous Tissue Mini Kit (QIAGEN, Germany), following the manufacturer’s instructions ( ). .. Tissue homogenization was carried out in RLT buffer containing guanidine thiocyanate using a rotor-stator homogenizer (FastPrep-24, MP Biomedicals, USA). .. The homogenate was centrifuged at 10,500 × g for 15 minutes at 4°C (Pico 21, Thermo Scientific, USA), and the resulting supernatant was applied to RNeasy spin columns.
Article Title: Vitamin D enrichment of eggs and its effects on performance and intestinal health in late-stage laying hens.
Article Snippet: Total RNA was extracted using the RNeasy Fibrous Tissue Mini Kit (QIAGEN, Germany), following the manufacturer’s instructions (QIAGEN, 2021). .. Tissue homogenization was carried out in RLT buffer containing guanidine thiocyanate using a rotor-stator homogenizer (FastPrep-24, MP Biomedicals, USA). .. The homogenate was centrifuged at 10,500 × g for 15 minutes at 4◦C (Pico 21, Thermo Scientific, USA), and the resulting supernatant was applied to RNeasy spin columns.
Incubation:Article Title: Differential effect of lead and cadmium on mitochondrial function and NLRP3 inflammasome activation in human trophoblast
Article Snippet: .. Then, the remaining interphase/organic phase was incubated with a back extraction buffer (BEB) containing 4 M guanidine thiocyanate (MP Biomedicals, Santa Clara, CA, USA, cat. no. IC820991), 50 mM sodium citrate (Sigma-Aldrich, cat. no. C8532), 1 M Tris base (Sigma-Aldrich, Germany, cat. no. 648311), and 25 mL of ultrapure diethylpyrocarbonate (DEPC) water (Thermo Fisher Scientific, cat. no. AM9010) for 10 min at room temperature on a shaker. .. Next, the upper aqueous phase containing DNA was separated and mixed with 5 μL of linear acrylamide (Thermo Fisher Scientific, cat. no. AM9520) and 300 μL of 2-propanol (Chemie Bruschwig, Basel, Switzerland, cat. no. RH1018) to precipitate the DNA.
Article Title: Identification of novel genetic factors that regulate c-di-AMP production in Staphylococcus aureus using a riboswitch-based biosensor.
Article Snippet: .. 46.6-mL GTC buffer (5 M guanidine thiocyanate, 0.5% N-lauryl sarcosine, 0.1 M β-mercaptoethanol, 0.5% Tween-80, and 10 mM Tris pH 7.5) was added to a 20-mL culture aliquot, and bacteria were pelleted by centrifugation for 10 min at 8,300 × g. The pellet was suspended in 1-mL GTC buffer and transferred to a microfuge tube, the bacteria were collected again by centrifugation for 3 min at 17,000 × g, and the pellet was suspended in 1-mL RNApro reagent solution from the MPBio RNA isolation kit and incubated for 1 min on ice. .. Bacterial cells were lysed using a Fastprep-24 beat beater (MP Biomedicals), and the RNA was extracted using the MPBio RNA kit according to the manufacturer’s instructions.
Extraction:Article Title: Differential effect of lead and cadmium on mitochondrial function and NLRP3 inflammasome activation in human trophoblast
Article Snippet: .. Then, the remaining interphase/organic phase was incubated with a back extraction buffer (BEB) containing 4 M guanidine thiocyanate (MP Biomedicals, Santa Clara, CA, USA, cat. no. IC820991), 50 mM sodium citrate (Sigma-Aldrich, cat. no. C8532), 1 M Tris base (Sigma-Aldrich, Germany, cat. no. 648311), and 25 mL of ultrapure diethylpyrocarbonate (DEPC) water (Thermo Fisher Scientific, cat. no. AM9010) for 10 min at room temperature on a shaker. .. Next, the upper aqueous phase containing DNA was separated and mixed with 5 μL of linear acrylamide (Thermo Fisher Scientific, cat. no. AM9520) and 300 μL of 2-propanol (Chemie Bruschwig, Basel, Switzerland, cat. no. RH1018) to precipitate the DNA.
Bacteria:Article Title: Identification of novel genetic factors that regulate c-di-AMP production in Staphylococcus aureus using a riboswitch-based biosensor.
Article Snippet: .. 46.6-mL GTC buffer (5 M guanidine thiocyanate, 0.5% N-lauryl sarcosine, 0.1 M β-mercaptoethanol, 0.5% Tween-80, and 10 mM Tris pH 7.5) was added to a 20-mL culture aliquot, and bacteria were pelleted by centrifugation for 10 min at 8,300 × g. The pellet was suspended in 1-mL GTC buffer and transferred to a microfuge tube, the bacteria were collected again by centrifugation for 3 min at 17,000 × g, and the pellet was suspended in 1-mL RNApro reagent solution from the MPBio RNA isolation kit and incubated for 1 min on ice. .. Bacterial cells were lysed using a Fastprep-24 beat beater (MP Biomedicals), and the RNA was extracted using the MPBio RNA kit according to the manufacturer’s instructions.
Centrifugation:Article Title: Identification of novel genetic factors that regulate c-di-AMP production in Staphylococcus aureus using a riboswitch-based biosensor.
Article Snippet: .. 46.6-mL GTC buffer (5 M guanidine thiocyanate, 0.5% N-lauryl sarcosine, 0.1 M β-mercaptoethanol, 0.5% Tween-80, and 10 mM Tris pH 7.5) was added to a 20-mL culture aliquot, and bacteria were pelleted by centrifugation for 10 min at 8,300 × g. The pellet was suspended in 1-mL GTC buffer and transferred to a microfuge tube, the bacteria were collected again by centrifugation for 3 min at 17,000 × g, and the pellet was suspended in 1-mL RNApro reagent solution from the MPBio RNA isolation kit and incubated for 1 min on ice. .. Bacterial cells were lysed using a Fastprep-24 beat beater (MP Biomedicals), and the RNA was extracted using the MPBio RNA kit according to the manufacturer’s instructions.
Isolation:Article Title: Identification of novel genetic factors that regulate c-di-AMP production in Staphylococcus aureus using a riboswitch-based biosensor.
Article Snippet: .. 46.6-mL GTC buffer (5 M guanidine thiocyanate, 0.5% N-lauryl sarcosine, 0.1 M β-mercaptoethanol, 0.5% Tween-80, and 10 mM Tris pH 7.5) was added to a 20-mL culture aliquot, and bacteria were pelleted by centrifugation for 10 min at 8,300 × g. The pellet was suspended in 1-mL GTC buffer and transferred to a microfuge tube, the bacteria were collected again by centrifugation for 3 min at 17,000 × g, and the pellet was suspended in 1-mL RNApro reagent solution from the MPBio RNA isolation kit and incubated for 1 min on ice. .. Bacterial cells were lysed using a Fastprep-24 beat beater (MP Biomedicals), and the RNA was extracted using the MPBio RNA kit according to the manufacturer’s instructions.
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