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flurofamide  (Tocris)


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    Tocris flurofamide
    Figure 3. Time course of bacteria load of Kato III cells incubated at 37C with parental strain N6, either pretreated or not pretreated with the urease inhibitors acetohydroxamic acid (AHA) or flurofamide (FF), and the isogenic mutants N6ureB::TnKm and N6ureG::TnKm (n p 5 experiments per group). Statistical analysis was done using Bonferroni’s test. Bars, SE. * , N6ureB::TnKm vs. all other strains. P ! .001
    Flurofamide, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0478/Flurofamide/pm11471101-77-31-34
    Average 94 stars, based on 7 article reviews
    flurofamide - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Urease prevents adherence of Helicobacter pylori to Kato III gastric epithelial cells."

    Article Title: Urease prevents adherence of Helicobacter pylori to Kato III gastric epithelial cells.

    Journal: The Journal of infectious diseases

    doi: 10.1086/322776

    Figure 3. Time course of bacteria load of Kato III cells incubated at 37C with parental strain N6, either pretreated or not pretreated with the urease inhibitors acetohydroxamic acid (AHA) or flurofamide (FF), and the isogenic mutants N6ureB::TnKm and N6ureG::TnKm (n p 5 experiments per group). Statistical analysis was done using Bonferroni’s test. Bars, SE. * , N6ureB::TnKm vs. all other strains. P ! .001
    Figure Legend Snippet: Figure 3. Time course of bacteria load of Kato III cells incubated at 37C with parental strain N6, either pretreated or not pretreated with the urease inhibitors acetohydroxamic acid (AHA) or flurofamide (FF), and the isogenic mutants N6ureB::TnKm and N6ureG::TnKm (n p 5 experiments per group). Statistical analysis was done using Bonferroni’s test. Bars, SE. * , N6ureB::TnKm vs. all other strains. P ! .001

    Techniques Used: Bacteria, Incubation

    Related Articles

    Injection:

    Article Title: Real-time imaging of trapping and urease-dependent transmigration of Cryptococcus neoformans in mouse brain
    Article Snippet: .. In some experiments, groups of mice were i.p. injected with 1.5 mg (per mouse) of the urease inhibitor flurofamide (Tocris Bioscience) dissolved in DMSO, or DMSO alone as control. ..

    Article Title: Real-time imaging of trapping and urease-dependent transmigration of Cryptococcus neoformans in mouse brain
    Article Snippet: .. In some experiments, groups of mice were i.p. injected with 1.5 mg (per mouse) of the urease inhibitor flurofamide (Tocris Bioscience) dissolved in DMSO, or DMSO alone as control. ..

    Control:

    Article Title: Real-time imaging of trapping and urease-dependent transmigration of Cryptococcus neoformans in mouse brain
    Article Snippet: .. In some experiments, groups of mice were i.p. injected with 1.5 mg (per mouse) of the urease inhibitor flurofamide (Tocris Bioscience) dissolved in DMSO, or DMSO alone as control. ..

    Article Title: Real-time imaging of trapping and urease-dependent transmigration of Cryptococcus neoformans in mouse brain
    Article Snippet: .. In some experiments, groups of mice were i.p. injected with 1.5 mg (per mouse) of the urease inhibitor flurofamide (Tocris Bioscience) dissolved in DMSO, or DMSO alone as control. ..

    Bacteria:

    Article Title: Urease prevents adherence of Helicobacter pylori to Kato III gastric epithelial cells.
    Article Snippet: .. PKH2stained bacteria were incubated in parallel in RPMI 1640 (15 min, room temperature) with each of the following urease activity inhibitors: acetohydroxamic acid (1 mM; Sigma-Aldrich), completely inhibiting enzymatic activity, and flurofamide (1 mM; Tocris), inhibiting surface-associated urease activity [20, 23, 24]. ..

    Incubation:

    Article Title: Urease prevents adherence of Helicobacter pylori to Kato III gastric epithelial cells.
    Article Snippet: .. PKH2stained bacteria were incubated in parallel in RPMI 1640 (15 min, room temperature) with each of the following urease activity inhibitors: acetohydroxamic acid (1 mM; Sigma-Aldrich), completely inhibiting enzymatic activity, and flurofamide (1 mM; Tocris), inhibiting surface-associated urease activity [20, 23, 24]. ..

    Activity Assay:

    Article Title: Urease prevents adherence of Helicobacter pylori to Kato III gastric epithelial cells.
    Article Snippet: .. PKH2stained bacteria were incubated in parallel in RPMI 1640 (15 min, room temperature) with each of the following urease activity inhibitors: acetohydroxamic acid (1 mM; Sigma-Aldrich), completely inhibiting enzymatic activity, and flurofamide (1 mM; Tocris), inhibiting surface-associated urease activity [20, 23, 24]. ..



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    Image Search Results


    Figure 3. Urease regulates viability and pH modulation in urease-positive Blautia isolates. a) viability and b) acidification of urease- encoding Blautia isolates in neutral or acidic starting pH conditions with indicated urea supplementation. c) Viability and acidification of urease positive blautia in neutral conditions with urea supplementation and urease inhibitor (flurofamide) or vehicle (DMSO). Data points represent individual samples from independent replicates. (*p < 0.05, **p < 0.01, ***p < 0.001).

    Journal: Gut microbes

    Article Title: Urease in acetogenic Lachnospiraceae drives urea carbon salvage in SCFA pools.

    doi: 10.1080/19490976.2025.2492376

    Figure Lengend Snippet: Figure 3. Urease regulates viability and pH modulation in urease-positive Blautia isolates. a) viability and b) acidification of urease- encoding Blautia isolates in neutral or acidic starting pH conditions with indicated urea supplementation. c) Viability and acidification of urease positive blautia in neutral conditions with urea supplementation and urease inhibitor (flurofamide) or vehicle (DMSO). Data points represent individual samples from independent replicates. (*p < 0.05, **p < 0.01, ***p < 0.001).

    Article Snippet: Addition of urea did not alter measured pH. iii) Urease inhibitor experiments: for conditions with flurofamide (Tocris biosciences),37 assay broths were supplemented with 3 μg/mL flurofamide dissolved in 80% DMSO, 80% DMSO as vehicle control, or no additional treatment. iv) 13C-labeled urea and formate: 13C-labeled urea (Sigma-Aldrich), urea (Bioshop Canada Inc.), 13C-labeled formate (SigmaAldrich) or formate (Fisher Scientific) were used to supplement BHIS broth at the indicated pH at a concentration of 10 mM.

    Techniques: