paraformaldehyde (Thermo Fisher)

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Thermo Fisher paraformaldehyde
Paraformaldehyde, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/047317%2E9l/Paraformaldehyde%2C+10%25+w%2Fv+aq%2E+soln%2E%2C+methanol+free/pmc09166566-149-17-28
Average 94 stars, based on 1 article reviews

paraformaldehyde - by Bioz Stars, 2026-09

94/100 stars

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Staining:

Article Title: BCG immunisation through intranasal instillation protects mice against Streptococcus pneumoniae infection by enhancing alveolar macrophage activity.
Article Snippet: .. Cells were then fixed with 10% paraformaldehyde for 15 min, stained with Hoechst 33342 (Invitrogen) to visualise nuclei, and washed with PBS. ..

Article Title: Epigenetic reprogramming of T cell metabolism restores function and enhances anti-tumor immunity in lung cancer.
Article Snippet: T cell exhaustion represents a critical target for immunotherapy in cancer.. Nevertheless, T cells exhibit diminished responsiveness to immune checkpoint inhibitors once they transition to a terminally exhausted state.. Here we used an epigenetic drug screen and identified bromodomain and extra-terminal motif inhibitors (BETis) as enhancers of effector functions in primary exhausted T cells (TEX) from malignant pleural effusions in patients with lung c an ce r. T ra ns cr ip to mics, metabolomics and ATAC-seq analyses revealed that BETis reinvigorate TEX cells by activating the polyamine biosynthesis pathway, expanding intracellular polyamine pools and altering chromatin accessibility.

Concentration Assay:

Article Title: Histone methyltransferase DOT1L differentially affects the development of dendritic cell subsets
Article Snippet: .. Sorted cells were cross-linked for 10 min at room temperature in RPMI supplemented with 1% methanol-free formaldehyde (Thermo Fisher Scientific) and subsequently quenched by adding glycine (final concentration 125 mM) for 5 min. .. Samples were then washed twice using PBS + protease inhibitor cocktail (PIC; Roche) + 10% FBS (Capricorn), aspirated, and resuspended in ice-cold nucleus lysis buffer (50 mM Tris–HCl, pH 8, 10 mM EDTA, pH 8, 1% SDS + PIC) for 10 min.

Blocking Assay:

Article Title: DNA damage drives a unique, Alzheimer’s disease-relevant senescent state in neurons
Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 15 min, and then washed three times with PBS followed by permeabilization and blocking with 10% normal goat serum (Life Technologies, 50062Z) in 0.1% Triton X-100 (Sigma-Aldrich, X100RS-5G) for 1h. .. Cells were then incubated with primary antibodies at 4°C overnight (γH2AX, abcam, ab81299, 1:250; 1:1000; pATM, Life Technologies, MA1-2020, 1:250; p21, abcam, ab109520, 1:100; p16, abcam, ab270058, 1:50; LMNB1, abcam, ab229025, 1:1000; HMGB1, abcam, ab79823, 1:250; 53BP1, abcam, ab175933, 1:200, PSD95, Life Technologies, MA1-046, 1:300; MAP2, Novus Biologicals, NB300-213, 1:400).

Modification:

Article Title: Olfactory decline tracks central‐to‐peripheral spread of tau pathology in Alzheimer's disease
Article Snippet: .. One swab was placed in 1 mL of 4% paraformaldehyde; the other in 1 mL chilled Dulbecco's Modified Eagle Medium/F12 (Thermo Fisher Scientific, Gibco 11039021) with 10% fetal bovine serum (Cytiva, SV30160.03), 1% penicillin/streptomycin solution (Capricorn Scientific, PS‐B), protease inhibitors (1:25; Roche, 11836170001), and phosphatase inhibitors (1:20; Roche, 4906837001). ..

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