5 ht2 receptor antagonist cinanserin hydrochloride  (Tocris)

Journal: Physiological Reports

Article Title: Induction of cardiac alternans in human iPS ‐derived cardiomyocytes through β‐adrenergic receptor stimulation

doi: 10.14814/phy2.70152

Figure Lengend Snippet: Experimental condition and observed responses in this study using cardiac cell sheet tissue under atmospheric condition.

Article Snippet: Isoproterenol (I0260; Tokyo Chemical Industry, Tokyo, Japan), propranolol (163‐24501; Fujifilm Wako Pure Chemical Corporation), verapamil (222‐00781; Fujifilm Wako Pure Chemical Corporation), ryanodine (ab120083; Abcam, Cambridge, UK), thapsigargin (10522; Cayman Chemical, MI, USA), omecamtiv mecarbil (CS‐0460; Chemscene LCC, NJ, USA), ivabradine (098–06921; Fujifilm Wako Pure Chemical Corporation), and blebbistatin (ax494682; Axol Bioscience) were dissolved in DMSO (031‐24051; Fujifilm Wako Pure Chemical Corporation) at concentrations 1000 times higher than those of the exposure test; the compound solutions were added to the medium in the tissue bath and diluted appropriately.

Techniques:

Journal: Physiological Reports

Article Title: Induction of cardiac alternans in human iPS ‐derived cardiomyocytes through β‐adrenergic receptor stimulation

doi: 10.14814/phy2.70152

Figure Lengend Snippet: Effects of omecamtiv mecarbil on contractility of cardiac cell sheet tissues cultured under atmospheric condition. (a) and (b) show typical contraction waveform of cardiac cell sheet tissue, cultured under atmospheric condition using Medium 199 Hanks' salt medium, before (baseline) and after 30 min to 1000 nM omecamtiv mecarbil. (c) and (d) show the S/L ratio (each sample data) and CA (contraction amplitude) value (each sample data) (S/L ratio [baseline]; mean ± S.D. = 1.00 ± 0.01, S/L ratio [isoproterenol]; 1.00 ± 0.01, CA [isoproterenol]; 1.38 ± 0.14, n = 5). CA was normalized to baseline.

Article Snippet: Isoproterenol (I0260; Tokyo Chemical Industry, Tokyo, Japan), propranolol (163‐24501; Fujifilm Wako Pure Chemical Corporation), verapamil (222‐00781; Fujifilm Wako Pure Chemical Corporation), ryanodine (ab120083; Abcam, Cambridge, UK), thapsigargin (10522; Cayman Chemical, MI, USA), omecamtiv mecarbil (CS‐0460; Chemscene LCC, NJ, USA), ivabradine (098–06921; Fujifilm Wako Pure Chemical Corporation), and blebbistatin (ax494682; Axol Bioscience) were dissolved in DMSO (031‐24051; Fujifilm Wako Pure Chemical Corporation) at concentrations 1000 times higher than those of the exposure test; the compound solutions were added to the medium in the tissue bath and diluted appropriately.

Techniques: Cell Culture

Journal: European journal of pharmacology

Article Title: Is there a role for biogenic amine receptors in mediating β-phenylethylamine and RO5256390-induced vascular contraction?

doi: 10.1016/j.ejphar.2024.176895

Figure Lengend Snippet: Fig. 3. The effect of 5-HT receptor antagonism (A, B) on 5-HT and β-PEA CRCs5-HT CRCs were obtained (A) in the absence (● n = 10) or presence (○ n = 6) of cinanserin. β-PEA CRCs were obtained (B) in the absence (● n = 18) or presence (○ n = 5) of 100 nM cinanserin. Contractile responses are reported as the mean percentage (±SEM) of the maximum contractile response to 60 mM KCl.

Article Snippet: (R)- (− )-phenylephrine hydrochloride (PE), serotonin hydrochloride (5-hydroxytryptamine, 5-HT), cinanserin hydrochloride, carbamoylcholine chloride (carbachol) atipamezole hydrochloride, medetomidine hydrochloride and clonidine hydrochloride were purchased from Tocris Bioscience (Abingdon, United Kingdom).

Techniques: