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leu leu ome hcl  (Chem Impex International)


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    Structured Review

    Chem Impex International leu leu ome hcl
    Leu Leu Ome Hcl, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/04578/Leu-Leu-OMe%C2%B7HCl/pmc04530060-60-29-32
    Average 95 stars, based on 8 article reviews
    leu leu ome hcl - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Tube Formation Assay:

    Article Title: BTK operates a phospho-tyrosine switch to regulate NLRP3 inflammasome activity.
    Article Snippet: .. ASC speck formation assay and confocal microscopy As described in Stutz et al. (2013) and Liu et al. (2017), Nlrp3 KO iMacs overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or solvent control for 10 min before stimulation with either 5 μM nigericin (Life Technologies) for 60 min or 1 mM Leu-Leu-OMe·HCl (Chem-Impex) for 90 min. After stimulation, cells were fixed with 4% formaldehyde, and Bittner et al. Journal of Experimental Medicine 11 of 17 BTK phosphorylates NLRP3, regulating its activity https://doi.org/10.1084/jem.20201656 D ow nloaded from http://rupress.org/jem /article-pdf/218/11/e20201656/1767017/jem _20201656.pdf by guest on 27 M arch 2024 nucleic acids were stained with DRAQ5 (eBioscience). .. Cells were imaged with a Zeiss Observer.Z1 epifluorescence microscope using a 20× objective as described in Stutz et al. (2013).

    Confocal Microscopy:

    Article Title: BTK operates a phospho-tyrosine switch to regulate NLRP3 inflammasome activity.
    Article Snippet: .. ASC speck formation assay and confocal microscopy As described in Stutz et al. (2013) and Liu et al. (2017), Nlrp3 KO iMacs overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or solvent control for 10 min before stimulation with either 5 μM nigericin (Life Technologies) for 60 min or 1 mM Leu-Leu-OMe·HCl (Chem-Impex) for 90 min. After stimulation, cells were fixed with 4% formaldehyde, and Bittner et al. Journal of Experimental Medicine 11 of 17 BTK phosphorylates NLRP3, regulating its activity https://doi.org/10.1084/jem.20201656 D ow nloaded from http://rupress.org/jem /article-pdf/218/11/e20201656/1767017/jem _20201656.pdf by guest on 27 M arch 2024 nucleic acids were stained with DRAQ5 (eBioscience). .. Cells were imaged with a Zeiss Observer.Z1 epifluorescence microscope using a 20× objective as described in Stutz et al. (2013).

    Solvent:

    Article Title: BTK operates a phospho-tyrosine switch to regulate NLRP3 inflammasome activity.
    Article Snippet: .. ASC speck formation assay and confocal microscopy As described in Stutz et al. (2013) and Liu et al. (2017), Nlrp3 KO iMacs overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or solvent control for 10 min before stimulation with either 5 μM nigericin (Life Technologies) for 60 min or 1 mM Leu-Leu-OMe·HCl (Chem-Impex) for 90 min. After stimulation, cells were fixed with 4% formaldehyde, and Bittner et al. Journal of Experimental Medicine 11 of 17 BTK phosphorylates NLRP3, regulating its activity https://doi.org/10.1084/jem.20201656 D ow nloaded from http://rupress.org/jem /article-pdf/218/11/e20201656/1767017/jem _20201656.pdf by guest on 27 M arch 2024 nucleic acids were stained with DRAQ5 (eBioscience). .. Cells were imaged with a Zeiss Observer.Z1 epifluorescence microscope using a 20× objective as described in Stutz et al. (2013).

    Article Title: Human NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome activity is regulated by and potentially targetable through Bruton tyrosine kinase.
    Article Snippet: 4x10 4 Human embryonic kidney (HEK) 293T 209 cells were plated, transiently transfected, fixed with 100% methanol, stained for nucleic acids (To-210 pro-3, Thermo Fisher) and BTK-HA (anti-HA-Alexa 594). .. Immortalized Nlrp3 KO macrophages 211 overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or CGI1745 (and a 212 solvent control) for 10 min or 60 min, respectively, before stimulation with either 5 μM Nigericin (Life 213 Technologies) for 60 min or 1 mM Leu-Leu-OMe HCl (Chem-Impex) for 90 min, then fixed (4% 214 formaldehyde), and nucleic acids stained (DRAQ5, eBioscience). .. Details regarding analysis using a 215 Zeiss confocal microscope and image quantification using ImageJ or CellProfiler are described in 216 Supplemental Information.

    Control:

    Article Title: BTK operates a phospho-tyrosine switch to regulate NLRP3 inflammasome activity.
    Article Snippet: .. ASC speck formation assay and confocal microscopy As described in Stutz et al. (2013) and Liu et al. (2017), Nlrp3 KO iMacs overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or solvent control for 10 min before stimulation with either 5 μM nigericin (Life Technologies) for 60 min or 1 mM Leu-Leu-OMe·HCl (Chem-Impex) for 90 min. After stimulation, cells were fixed with 4% formaldehyde, and Bittner et al. Journal of Experimental Medicine 11 of 17 BTK phosphorylates NLRP3, regulating its activity https://doi.org/10.1084/jem.20201656 D ow nloaded from http://rupress.org/jem /article-pdf/218/11/e20201656/1767017/jem _20201656.pdf by guest on 27 M arch 2024 nucleic acids were stained with DRAQ5 (eBioscience). .. Cells were imaged with a Zeiss Observer.Z1 epifluorescence microscope using a 20× objective as described in Stutz et al. (2013).

    Article Title: Human NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome activity is regulated by and potentially targetable through Bruton tyrosine kinase.
    Article Snippet: 4x10 4 Human embryonic kidney (HEK) 293T 209 cells were plated, transiently transfected, fixed with 100% methanol, stained for nucleic acids (To-210 pro-3, Thermo Fisher) and BTK-HA (anti-HA-Alexa 594). .. Immortalized Nlrp3 KO macrophages 211 overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or CGI1745 (and a 212 solvent control) for 10 min or 60 min, respectively, before stimulation with either 5 μM Nigericin (Life 213 Technologies) for 60 min or 1 mM Leu-Leu-OMe HCl (Chem-Impex) for 90 min, then fixed (4% 214 formaldehyde), and nucleic acids stained (DRAQ5, eBioscience). .. Details regarding analysis using a 215 Zeiss confocal microscope and image quantification using ImageJ or CellProfiler are described in 216 Supplemental Information.

    Activity Assay:

    Article Title: BTK operates a phospho-tyrosine switch to regulate NLRP3 inflammasome activity.
    Article Snippet: .. ASC speck formation assay and confocal microscopy As described in Stutz et al. (2013) and Liu et al. (2017), Nlrp3 KO iMacs overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or solvent control for 10 min before stimulation with either 5 μM nigericin (Life Technologies) for 60 min or 1 mM Leu-Leu-OMe·HCl (Chem-Impex) for 90 min. After stimulation, cells were fixed with 4% formaldehyde, and Bittner et al. Journal of Experimental Medicine 11 of 17 BTK phosphorylates NLRP3, regulating its activity https://doi.org/10.1084/jem.20201656 D ow nloaded from http://rupress.org/jem /article-pdf/218/11/e20201656/1767017/jem _20201656.pdf by guest on 27 M arch 2024 nucleic acids were stained with DRAQ5 (eBioscience). .. Cells were imaged with a Zeiss Observer.Z1 epifluorescence microscope using a 20× objective as described in Stutz et al. (2013).

    Article Title: RAB20 deficiency promotes the development of silicosis via NLRP3 inflammasome
    Article Snippet: Then, cells were centrifuged at 800 rpm and were resuspended in RPMI 1640 medium supplemented with 10% fetal bovine serum (GIBCO) and 1% penicillin–streptomycin (Thermo Fisher). .. Peritoneal macrophages (10 6 cells per well) were plated in 12-well plates and then primed with ultra-pure LPS (100 ng/ml) for 4 h, and then stimulated with SiO 2 crystal (20 μg/ml), APC-connected silica crystals (20 μg/ml), or liposome-coated silica crystals (20 μg/ml) for 6 h. To induce lysosomal disruption, peritoneal macrophages were primed with ultra-pure LPS (1 ng/ml) for 4 h and then were stimulated with Leu-Leu-OMe·HCl (1,000 μM, Chem-Impex International) for 5 h. To inhibit the activity of cathepsin B, peritoneal macrophages were primed with 100 μM CA-074Me (Selleck) overnight before ultra-pure LPS priming. ..

    Article Title: RAB20 deficiency promotes the development of silicosis via NLRP3 inflammasome.
    Article Snippet: Then, cells were centrifuged at 800 rpm and were resuspended in RPMI 1640medium supplemented with 10% fetal bovine serum (GIBCO) and 1% penicillin–streptomycin (Thermo Fisher). .. Peritoneal macrophages (106 cells per well) were plated in 12-well plates and then primed with ultra-pure LPS (100 ng/ml) for 4 h, and then stimulated with SiO2 crystal (20 mg/ml), APC-connected silica crystals (20 mg/ml), or liposomecoated silica crystals (20 mg/ml) for 6 h. To induce lysosomal disruption, peritoneal macrophages were primed with ultra-pure LPS (1 ng/ml) for 4 h and then were stimulated with Leu-LeuOMe·HCl (1,000 mM, Chem-Impex International) for 5 h. To inhibit the activity of cathepsin B, peritoneal macrophages were primed with 100 mM CA-074Me (Selleck) overnight before ultrapure LPS priming. ..

    Staining:

    Article Title: BTK operates a phospho-tyrosine switch to regulate NLRP3 inflammasome activity.
    Article Snippet: .. ASC speck formation assay and confocal microscopy As described in Stutz et al. (2013) and Liu et al. (2017), Nlrp3 KO iMacs overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or solvent control for 10 min before stimulation with either 5 μM nigericin (Life Technologies) for 60 min or 1 mM Leu-Leu-OMe·HCl (Chem-Impex) for 90 min. After stimulation, cells were fixed with 4% formaldehyde, and Bittner et al. Journal of Experimental Medicine 11 of 17 BTK phosphorylates NLRP3, regulating its activity https://doi.org/10.1084/jem.20201656 D ow nloaded from http://rupress.org/jem /article-pdf/218/11/e20201656/1767017/jem _20201656.pdf by guest on 27 M arch 2024 nucleic acids were stained with DRAQ5 (eBioscience). .. Cells were imaged with a Zeiss Observer.Z1 epifluorescence microscope using a 20× objective as described in Stutz et al. (2013).

    Article Title: Human NACHT, LRR, and PYD domain-containing protein 3 (NLRP3) inflammasome activity is regulated by and potentially targetable through Bruton tyrosine kinase.
    Article Snippet: 4x10 4 Human embryonic kidney (HEK) 293T 209 cells were plated, transiently transfected, fixed with 100% methanol, stained for nucleic acids (To-210 pro-3, Thermo Fisher) and BTK-HA (anti-HA-Alexa 594). .. Immortalized Nlrp3 KO macrophages 211 overexpressing NLRP3-FLAG and ASC-mCerulean were pretreated with ibrutinib or CGI1745 (and a 212 solvent control) for 10 min or 60 min, respectively, before stimulation with either 5 μM Nigericin (Life 213 Technologies) for 60 min or 1 mM Leu-Leu-OMe HCl (Chem-Impex) for 90 min, then fixed (4% 214 formaldehyde), and nucleic acids stained (DRAQ5, eBioscience). .. Details regarding analysis using a 215 Zeiss confocal microscope and image quantification using ImageJ or CellProfiler are described in 216 Supplemental Information.

    Membrane:

    Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage.
    Article Snippet: .. To induce endolysosomal membrane damage, NLRP6-expressing cells were treated with 0.5 mM LLOMe (CHEM-IMPEX INT’L INC #04578), 200 μM GPN (Abcam #ab145914), 100 μM MSU (Invivogen #tlrlmsu) for the indicated time points, after which cells and/or cell supernatants were processed for immunoblot or cell permeabilization/death analysis. .. Where indicated, cells were treated with 10 μM MCC950 (Invivogen #inh-mcc) for 30 min prior to stimulation with L. monocytogenes or LLOMe in the presence of 10 μM MCC950.

    Article Title: Sterile- or pathogen-induced endolysosomal damage activate the NLRP6 inflammasome in human intestinal epithelial cells
    Article Snippet: .. To induce endo-lysosomal membrane damage, NLRP6-expressing cells were treated with 0.5mM LLOMe (CHEM-IMPEX INT’L INC #04578), 200μM GPN (Abcam #ab145914), 100μM MSU (Invivogen #tlrl-msu) for indicated time points, after which cells and/or cell supernatants were processed for immunoblot or cell permeabilization/death analysis. .. For ligand stimulation studies, unlabelled LTA (from S. aureus , Invivogen #tlrl-pslta) was transfected into cells at 10ug/300.000 cells, while BODIPY labelled LTA (labelling protocol below) was transfected at a theoretical concentration of 7.5ug/300.000 cells using DOTAP transfection reagent (Roche #11202375001).

    Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage
    Article Snippet: .. To induce endolysosomal membrane damage, NLRP6-expressing cells were treated with 0.5 mM LLOMe (CHEM-IMPEX INT’L INC #04578), 200 μM GPN (Abcam #ab145914), 100 μM MSU (Invivogen #tlrl-msu) for the indicated time points, after which cells and/or cell supernatants were processed for immunoblot or cell permeabilization/death analysis. .. Where indicated, cells were treated with 10 μM MCC950 (Invivogen #inh-mcc) for 30 min prior to stimulation with L. monocytogenes or LLOMe in the presence of 10 μM MCC950.

    Western Blot:

    Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage.
    Article Snippet: .. To induce endolysosomal membrane damage, NLRP6-expressing cells were treated with 0.5 mM LLOMe (CHEM-IMPEX INT’L INC #04578), 200 μM GPN (Abcam #ab145914), 100 μM MSU (Invivogen #tlrlmsu) for the indicated time points, after which cells and/or cell supernatants were processed for immunoblot or cell permeabilization/death analysis. .. Where indicated, cells were treated with 10 μM MCC950 (Invivogen #inh-mcc) for 30 min prior to stimulation with L. monocytogenes or LLOMe in the presence of 10 μM MCC950.

    Article Title: Sterile- or pathogen-induced endolysosomal damage activate the NLRP6 inflammasome in human intestinal epithelial cells
    Article Snippet: .. To induce endo-lysosomal membrane damage, NLRP6-expressing cells were treated with 0.5mM LLOMe (CHEM-IMPEX INT’L INC #04578), 200μM GPN (Abcam #ab145914), 100μM MSU (Invivogen #tlrl-msu) for indicated time points, after which cells and/or cell supernatants were processed for immunoblot or cell permeabilization/death analysis. .. For ligand stimulation studies, unlabelled LTA (from S. aureus , Invivogen #tlrl-pslta) was transfected into cells at 10ug/300.000 cells, while BODIPY labelled LTA (labelling protocol below) was transfected at a theoretical concentration of 7.5ug/300.000 cells using DOTAP transfection reagent (Roche #11202375001).

    Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage
    Article Snippet: .. To induce endolysosomal membrane damage, NLRP6-expressing cells were treated with 0.5 mM LLOMe (CHEM-IMPEX INT’L INC #04578), 200 μM GPN (Abcam #ab145914), 100 μM MSU (Invivogen #tlrl-msu) for the indicated time points, after which cells and/or cell supernatants were processed for immunoblot or cell permeabilization/death analysis. .. Where indicated, cells were treated with 10 μM MCC950 (Invivogen #inh-mcc) for 30 min prior to stimulation with L. monocytogenes or LLOMe in the presence of 10 μM MCC950.

    other:

    Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage
    Article Snippet: LLOMe , CHEM-IMPEX INT’L INC , 04578.

    Disruption:

    Article Title: RAB20 deficiency promotes the development of silicosis via NLRP3 inflammasome
    Article Snippet: Then, cells were centrifuged at 800 rpm and were resuspended in RPMI 1640 medium supplemented with 10% fetal bovine serum (GIBCO) and 1% penicillin–streptomycin (Thermo Fisher). .. Peritoneal macrophages (10 6 cells per well) were plated in 12-well plates and then primed with ultra-pure LPS (100 ng/ml) for 4 h, and then stimulated with SiO 2 crystal (20 μg/ml), APC-connected silica crystals (20 μg/ml), or liposome-coated silica crystals (20 μg/ml) for 6 h. To induce lysosomal disruption, peritoneal macrophages were primed with ultra-pure LPS (1 ng/ml) for 4 h and then were stimulated with Leu-Leu-OMe·HCl (1,000 μM, Chem-Impex International) for 5 h. To inhibit the activity of cathepsin B, peritoneal macrophages were primed with 100 μM CA-074Me (Selleck) overnight before ultra-pure LPS priming. ..

    Article Title: RAB20 deficiency promotes the development of silicosis via NLRP3 inflammasome.
    Article Snippet: Then, cells were centrifuged at 800 rpm and were resuspended in RPMI 1640medium supplemented with 10% fetal bovine serum (GIBCO) and 1% penicillin–streptomycin (Thermo Fisher). .. Peritoneal macrophages (106 cells per well) were plated in 12-well plates and then primed with ultra-pure LPS (100 ng/ml) for 4 h, and then stimulated with SiO2 crystal (20 mg/ml), APC-connected silica crystals (20 mg/ml), or liposomecoated silica crystals (20 mg/ml) for 6 h. To induce lysosomal disruption, peritoneal macrophages were primed with ultra-pure LPS (1 ng/ml) for 4 h and then were stimulated with Leu-LeuOMe·HCl (1,000 mM, Chem-Impex International) for 5 h. To inhibit the activity of cathepsin B, peritoneal macrophages were primed with 100 mM CA-074Me (Selleck) overnight before ultrapure LPS priming. ..



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