hf buffer (Thermo Fisher)

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Thermo Fisher hf buffer
Hf Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/044627%2Eak/Buffer+HF-Improved/pmc06924956-92-167-169
Average 94 stars, based on 1 article reviews

hf buffer - by Bioz Stars, 2026-09

94/100 stars

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Generated:

Article Title: A conserved viral RNA fold enables nuclease resistance across kingdoms of life
Article Snippet: .. Transcription templates were generated by polymerase chain reaction (PCR) amplification using custom oligonucleotides (0.5 μM each) (IDT) (see ), Phusion Hot Start DNA polymerase (New England Biolabs), and HF Buffer (Thermo Fisher Scientific). .. Gene fragments (Twist Biosciences) served as the PCR templates.

Polymerase Chain Reaction:

Article Title: Bacterial and Fungal Community Responses to Long-Term Salinity Gradients in Natural Soils of Kazakhstan.
Article Snippet: .. PCR amplification was performed in a volume of 25 μL using Phusion® High-Fidelity DNA polymerase with HF Buffer (Thermo Scientific Baltics UAB, Vilnius, Lithuania). .. The resulting amplicons were purified using AMPure XP magnetic particles (Beckman Coulter, Beverly, MA, USA), after which the libraries were indexed using the Nextera XT Index Kit (Illumina, San Diego, CA, USA).

Article Title: GuFi phages represent the most prevalent viral family-level clusters in the human gut microbiome
Article Snippet: .. Amplification was performed using Phusion High Fidelity PCR Master Mix with HF Buffer (Thermo ScientificTM, F531L) with the following conditions: initial denaturation at 98°C for 1 min, 35 cycles of 98°C for 15 sec, primer-specific annealing temperature for 15 sec and 72°C for 30 sec, followed by a final extension at 72°C for 10 min. Up to 50ng of PCR products were visualized on a 1.5% agarose gel run at 120V for 40 min in TAE buffer, alongside a 1kbp DNA ladder (Promega, G5711). ..

Article Title: Effect of curcumin on the gut microbiota of patients with ulcerative colitis, Crohn's disease and healthy participants.
Article Snippet: .. Twenty nanograms of template DNA was added to the PCR mix: 6 μL 5× HF buffer (Thermo Scientific), 0.75 μL PCR Grade Nucleotide Mix (10 μM) (Thermo Scientific), 0.3 μL Phusion DNA Polymerase (2 U/μL), 18.95 μL nuclease free water, 1.5μl forward Index Primer (10 μM) and 1.5μl reversed Index Primer (10 μM). .. Thermal cycling was performed on a Biometra thermocycler using the following conditions: an initial denaturation at 98°C for 30 seconds; 25 cycles of 98°C for 10 seconds, 55°C for 20 seconds, and 72°C for 90 seconds; followed by a final elongation step at 72°C for 10 minutes[43].

Article Title: Biofilter performance, water quality, and microbial dynamics in RAS operated under high or low suspended solids load
Article Snippet: .. PCR reactions were performed with 0.2 mM of each dNTP (Thermo Scientific), 0.3 μM of each primer, 0.02 U/μL Phusion Hot Start DNA polymerase and accompanying HF reaction buffer (Thermo Scientific) in a total reaction volume of 25 μL, including 1 μL of the DNA extracts as template. .. The reactions were run at 98 ◦C for 1 min, followed by 36 cycles of 98 ◦C 15 s, 55 ◦C 20 s, and 72 ◦C 20 s, and a final elongation step at 72 ◦C for 5 min in a T100TM Thermal Cycler (BioRad).

Amplification:

Immunoprecipitation:

Article Title: APOE genotype differentially modulates prion pathology in a mouse model.
Article Snippet: .. To confirm the presence of the apoE protein in immunoprecipitated complexes, the nitrocellulose AR TIC LE IN PR ES S APOE Genotype Affects Prion Pathology Lizińczyk, AM et al. membranes were stripped with RestoreTM Western Blot Stripping Buffer (Thermo Fisher Scientific) and immunoblotted with anti-human ApoE goat polyclonal antibody (Table 1). ..

Western Blot:

Stripping:

other:

Article Title: Devices and methods for producing nucleic acids and proteins
Article Snippet: 2 μl error correction mix, containing up to 3 ORFs subfragments, are combined with 0.1 pmoles of each UTR, 1× Phusion HF Buffer (THERmO FISHER SCIENTIFIC®) and 0.8 U Phusion DNA Polymerase (THERMO FISHER SCIENTIFIC®) 0.2 mM dNTPs and 32.1 μl H2O.

Agarose Gel Electrophoresis:

Modification:

Article Title: Wound state monitoring by multiplexed, electrochemical, real-time, localized, inflammation-tracking nitric oxide sensor (MERLIN)
Article Snippet: .. MERLIN array was released from wafer by etching SiO 2 in buffered hydrofluoric acid (Thermo Scientific Chemicals, catalog no. 044627.K2) overnight and transferred onto glass slides for further chemical modification on electrodes. .. Electrochemical polymerization was performed by CV by using a PalmSens4 potentiostat (PalmSens BV).

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