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nov 2014  (Thermo Fisher)


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  • 96

    Structured Review

    Thermo Fisher nov 2014
    Nov 2014, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/044540/RETINOIC+ACID+97/10__1063_slash_1__1359143-20-7-39
    Average 96 stars, based on 1 article reviews
    nov 2014 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Inhibition:

    Article Title: Transplanted human striatal progenitors exhibit functional integration and modulate host circuitry in a Huntington's disease animal model.
    Article Snippet: Briefly, cells were plated at a density of 1 × 104 cells/cm2 on Cultrex-coated plates in complete mTeSRTM1 medium supplemented with 10 μM ROCK inhibitor Y-27632 (RI; StemCell Technologies, 72307). .. For neuronal induction cells were subjected to Dual-SMAD inhibition using 10 μM SB431542 and 500 nM LDN193189 (StemCell Technologies, 72234 and 72149), in DMEM/F12 (Life Technologies, 11320033) supplemented with N2 (Life Technologies, 17502048) and B27 without retinoic acid (RA; Life Technologies, 17504044) for a total of 12 days. .. Starting on DIV5, 200 ng/mL recombinant human SHH C-25 II (R&D Systems 464‐SH) and 100 ng/mL DKK-1 (PreproTech, 120‐30‐10UG) were added to the culture and maintained until DIV25.

    Expressing:

    Article Title: Human MAdCAM-1 antibodies as targeted immune modulators
    Article Snippet: In brief, flat bottom tissue culture plates (Corning; 353072) were coated overnight at 4 °C with 200ng of either anti-CD3 alone (OKT3; BioLegend; 317301), anti-CD3 + human MAdCAM-1.Fc (BioLegend; 797904), or anti-CD3 + anti-CD28 (9.3; Bio x Cell; BE0248) in HBSS (Wisent; 311–512 CL). .. The plates were washed with HBSS, followed by culture medium (X-VIVO 15 medium (Lonza; BE02-060 F) supplemented with 100nM retinoic acid (Thermo Fisher Scientific; 044540.77) which increases the expression of integrin α4β7 on stimulated T cells . .. The blocking ability of mAbs was assessed by dispensing 2 μg of each antibody, ontamalimab biosimilar (Bioss; BIO0223SM), mIgG 1 (Biolegend; 401402) or, hIgG 2 (Biolegend; 403601) into wells followed immediately by the addition of 1 × 10 5 CFSE (Thermo Fisher Scientific; C34554 ) labeled CD3 + T cells isolated from total peripheral blood mononucleocytes (PBMCs) using a T cell isolation kit (STEMCELL Technologies; 17951).

    Cell Culture:

    Article Title: Bioengineering of Heart–Brain Codevelopoid Model Via Trans-Germ-Layer Codevelopment Organoid Chip
    Article Snippet: The EBs were cultured in suspension in neural induction medium Dulbecco’s modified Eagle’s medium (DMEM)/F12(Gibco, USA), 1:100 N2 supplement (Gibco), 1:100 L-alanyl-L-glutamine (GlutaMAX) (Gibco), 1:100 minimum essential medium with non-essential aminos (MEM-NEAA; Gibco), and 1 mg∙mL–1 heparin (Gibco). .. The medium was exchanged daily for 5 d. On day 11, EBs with neuroepithelial identity were encapsulated in Matrigel and further cultured in neural differentiation medium comprising a ratio of DMEM/F12 and neurobasal (1:1) supplemented (Gibco) with 1:200 (v/v) N2 supplement, 1:100 (v/v) B27 supplement without retinoic acid (Gibco), 1:100 (v/v) GlutaMAX, 1:200 (v/v) MEM-NEAA, 1:4000 (v/v) insulin, and 100 μmol∙L–1 βmercaptoethanol(Merck, Germany) under stationary conditions for 4 d. On day 15, the culture medium in the dishes was replaced with neural maturation medium. .. The composition of the neural maturation medium was the same, except for the B27 supplement (Gibco) with vitamin acid.

    Article Title: Epigenetic–metabolic axis in the temporal scaling of mammalian cortical neurogenesis across species
    Article Snippet: All plasmids were purified using the NucleoBond Xtra Midi EF kit (Macherey–Nagel), ensuring endotoxin-free quality suitable for in vivo applications. .. For in vitro experiments, 2 × 105 cells were cultured in 4-well plates in 500 μL of culture medium consisting of 20 ng/mL human basic FGF (Peprotech), 1× B27 supplement without retinoic acid (Gibco), and DMEM/F12 with GlutaMAX (Gibco). ..

    Article Title: All-in-one generation and multiomic profiling of human whole brain organoid on a millifluidic plate
    Article Snippet: .. On day 11, EBs with neuroepithelium identity were encapsulated in 4 μL Matrigel each organoid (354277, Corning) and then transferred to ultra-low-attachment 6-well plate (CT-3526, Corning) and were further cultured in a induction medium comprising DMEM/F12 (11320082, Thermo Fisher Scientific) and Neurobasal (21103049, Thermo Fisher Scientific) (1:1) ratio supplemented with 1:200 (v/v) N2 supplement (17502048, Thermo Fisher Scientific), 1:100 (v/v) B27 supplement without retinoic acid (12587010; Thermo Fisher Scientific), 1:100 (v/v) GlutaMAX (35050061, Thermo Fisher Scientific), 1:200 (v/v) MEM-NEAA (11140050, Thermo Fisher Scientific), 12.5 μL insulin (I9278, Sigma), and 3.5 μL/mL β-mercaptoethanol (21985023, Sigma-Aldrich) in the stationary condition for 4 days. .. On day 15, the plates were transferred to an orbital shaker (HS-25A, MIULAB) rotating continuously at 80 rpm.

    Article Title: Recurrent DNA Break Clusters Regulated by Polymerase Theta Are Essential for Replication Stress Induced Copy Number Variation
    Article Snippet: .. Briefly, the ES cells were seeded onto plates coated with laminin and poly-L-ornithine (Sigma Alderich, P4967) and cultured in N2B27 medium, consisting of 50% DMEM/F12 (ThermoFisher Scientific, 11330057), 50% Neurobasal (ThermoFisher Scientific, 21103049), 1% modified N2 supplement (Gibco, 17502048), 2% B27 supplement without retinoic acid (ThermoFisher Scientific, 12587-010), and 1× Glutamax (ThermoFisher Scientific, 35050061) for seven days. ..

    In Vitro:

    Article Title: Epigenetic–metabolic axis in the temporal scaling of mammalian cortical neurogenesis across species
    Article Snippet: All plasmids were purified using the NucleoBond Xtra Midi EF kit (Macherey–Nagel), ensuring endotoxin-free quality suitable for in vivo applications. .. For in vitro experiments, 2 × 105 cells were cultured in 4-well plates in 500 μL of culture medium consisting of 20 ng/mL human basic FGF (Peprotech), 1× B27 supplement without retinoic acid (Gibco), and DMEM/F12 with GlutaMAX (Gibco). ..

    Modification:

    Article Title: Recurrent DNA Break Clusters Regulated by Polymerase Theta Are Essential for Replication Stress Induced Copy Number Variation
    Article Snippet: .. Briefly, the ES cells were seeded onto plates coated with laminin and poly-L-ornithine (Sigma Alderich, P4967) and cultured in N2B27 medium, consisting of 50% DMEM/F12 (ThermoFisher Scientific, 11330057), 50% Neurobasal (ThermoFisher Scientific, 21103049), 1% modified N2 supplement (Gibco, 17502048), 2% B27 supplement without retinoic acid (ThermoFisher Scientific, 12587-010), and 1× Glutamax (ThermoFisher Scientific, 35050061) for seven days. ..

    other:

    Article Title: Generation of Region-specific Airway Basal Stem Cells from Human Pluripotent Stem Cells via Regulation of BMP-NOGGIN Axis
    Article Snippet: For most of the experiments, definitive endoderm induction was performed in serum-free differentiation (SFD) media of DMEM/F12 (3:1) (Corning) supplemented with N2 Supplement (Thermo Fisher), B27 Supplement with retinoic acid (Thermo Fisher), ascorbic acid (50 μg/ml, MilliporeSigma), Glutamax (2 mM, Thermo Fisher), monothioglycerol (MilliporeSigma), 0.05% bovine serum albumin (BSA) (Thermo Fisher), 1% penicillin-streptomycin (Corning). hPSCs Cells were briefly dissociated into 2–10 small cell clusters and plated onto low attachment 6-well plates to form embryoid bodies in SFD media containing Y-27632, 10 μM, human BMP4, 0.5 ng/ml (R&D Systems); human bFGF, 2.5 ng/ml; human Activin A, 100 ng/ml (R&D Systems) for 64-72h.



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