Silver Staining:Article Title: Specific targeting of brain endothelial cells using enhancer AAV vectors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Ethidium Bromide (for maxiprep plasmids) Sigma CAS #: 1239-45-8 Isopropanol (for maxiprep plasmids) Fisher Scientific CAS #: 67-63-0 Phenol (for maxiprep plasmids) Fisher Scientific CAS #: 108-95-2 Chloroform (for maxiprep plasmids) Fisher Scientific CAS #: 67-66-3 Critical commercial assays Pierce Silver Stain Kit (AAV purification) Thermo Fisher Cat#: 24612 Deposited data CATlas http://catlas.org/mousebrain/#! .. RRID:SCR_018690 Mendeley Data: quantification and metadata This paper https://doi.org/10.17632/5kvfknrgng.1 UCSC Genome Browser http://genome.ucsc.edu/ RRID:SCR_005780 Experimental models: Cell lines Cells: AAVpro 293T (for virus packaging) Takara Bio RRID:CVCL_B0XW Experimental models: Organisms/strains Mouse: Tie2-Cre (B6.Cg-Tg(Tek-cre)1Ywa/J) The Jackson Laboratory RRID:IMSR_JAX:008863 Mouse: Ai9 (B6.Cg-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J) The Jackson Laboratory RRID:IMSR_JAX:007909 Mouse: 5xFAD (B6.Cg-Tg (APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax) The Jackson Laboratory RRID:MMRRC_034848-JAX Mouse: B6J (C57BL/6J) The Jackson Laboratory RRID:IMSR_JAX:000664 Oligonucleotides Fwd ITR primer, 50-GGAACCCCTAGTGATGGAGTT Aurnhammer et al.68 PMID: 22428977 Rev ITR primer, 50-CGGCCTCAGTGAGCGA Aurnhammer et al.68 PMID: 22428977 PowerUP SYBR green Master mix Thermo Fisher A46012 Recombinant DNA pUCmini-iCAP-PHP.eB plasmid (encodes engineered PHP.eB capsid protein) Addgene Cat#: 103005; RRID:Addgene_103005 pAAV2/8 (encodes AAV8 capsid protein) Addgene Cat#: 112864; RRID:Addgene_112864 pAAV-BR1 Körbelin et al.13 PMID: 27137490 Ad helper plasmid (mini-pHelper) This paper N/A pAAV-BEC enhancer-minBG-SYFP2 This paper Cat #: 236722; RRID:Addgene_236722 pAAV-minBG-SYFP2 This paper Cat #: 236723; RRID:Addgene_236723 pAAV-CAG-mTurquoise2 Addgene Cat#: 99122; RRID:Addgene_99122 pAAV-BEC enhancer-minBG-iCre This paper Cat #: 236724; RRID:Addgene_236724 pAAV-BEC enhancer-minBG-iCre(R279T)_4x6T This paper Cat #: 236727; RRID:Addgene_236727 P3-Alb-mScarlet Addgene Cat#: 183461; RRID:Addgene_183461 AiP14187 - pAAV-AiE0459m_3xC2-minBGiCre(R297T)-BGHpA (plasmid used to clone out attenuated iCre(R279T)) Addgene Cat#: 229920; RRID:Addgene_229920 AAV-GfaABC1D-ERCreER-4x6T (plasmid used to clone out 4x6T) Addgene Cat#: 196411; RRID:Addgene_196411 Software and algorithms ImageJ (v. 1.53c) https://imagej.net/ RRID:SCR_003070 Python Programming Language http://www.python.org/ RRID:SCR_008394 OpenCV http://opencv.org RRID:SCR_015526 MatPlotLib http://matplotlib.sourceforge.net RRID:SCR_008624 NumPy http://www.numpy.org RRID:SCR_008633 SimpleITK https://simpleitk.org/ RRID:SCR_024693 (Continued on next page) e2 Neuron 113, 1562–1578.e1–e6, May 21, 2025
Bioprocessing:Article Title: Specific targeting of brain endothelial cells using enhancer AAV vectors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Ethidium Bromide (for maxiprep plasmids) Sigma CAS #: 1239-45-8 Isopropanol (for maxiprep plasmids) Fisher Scientific CAS #: 67-63-0 Phenol (for maxiprep plasmids) Fisher Scientific CAS #: 108-95-2 Chloroform (for maxiprep plasmids) Fisher Scientific CAS #: 67-66-3 Critical commercial assays Pierce Silver Stain Kit (AAV purification) Thermo Fisher Cat#: 24612 Deposited data CATlas http://catlas.org/mousebrain/#! .. RRID:SCR_018690 Mendeley Data: quantification and metadata This paper https://doi.org/10.17632/5kvfknrgng.1 UCSC Genome Browser http://genome.ucsc.edu/ RRID:SCR_005780 Experimental models: Cell lines Cells: AAVpro 293T (for virus packaging) Takara Bio RRID:CVCL_B0XW Experimental models: Organisms/strains Mouse: Tie2-Cre (B6.Cg-Tg(Tek-cre)1Ywa/J) The Jackson Laboratory RRID:IMSR_JAX:008863 Mouse: Ai9 (B6.Cg-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J) The Jackson Laboratory RRID:IMSR_JAX:007909 Mouse: 5xFAD (B6.Cg-Tg (APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax) The Jackson Laboratory RRID:MMRRC_034848-JAX Mouse: B6J (C57BL/6J) The Jackson Laboratory RRID:IMSR_JAX:000664 Oligonucleotides Fwd ITR primer, 50-GGAACCCCTAGTGATGGAGTT Aurnhammer et al.68 PMID: 22428977 Rev ITR primer, 50-CGGCCTCAGTGAGCGA Aurnhammer et al.68 PMID: 22428977 PowerUP SYBR green Master mix Thermo Fisher A46012 Recombinant DNA pUCmini-iCAP-PHP.eB plasmid (encodes engineered PHP.eB capsid protein) Addgene Cat#: 103005; RRID:Addgene_103005 pAAV2/8 (encodes AAV8 capsid protein) Addgene Cat#: 112864; RRID:Addgene_112864 pAAV-BR1 Körbelin et al.13 PMID: 27137490 Ad helper plasmid (mini-pHelper) This paper N/A pAAV-BEC enhancer-minBG-SYFP2 This paper Cat #: 236722; RRID:Addgene_236722 pAAV-minBG-SYFP2 This paper Cat #: 236723; RRID:Addgene_236723 pAAV-CAG-mTurquoise2 Addgene Cat#: 99122; RRID:Addgene_99122 pAAV-BEC enhancer-minBG-iCre This paper Cat #: 236724; RRID:Addgene_236724 pAAV-BEC enhancer-minBG-iCre(R279T)_4x6T This paper Cat #: 236727; RRID:Addgene_236727 P3-Alb-mScarlet Addgene Cat#: 183461; RRID:Addgene_183461 AiP14187 - pAAV-AiE0459m_3xC2-minBGiCre(R297T)-BGHpA (plasmid used to clone out attenuated iCre(R279T)) Addgene Cat#: 229920; RRID:Addgene_229920 AAV-GfaABC1D-ERCreER-4x6T (plasmid used to clone out 4x6T) Addgene Cat#: 196411; RRID:Addgene_196411 Software and algorithms ImageJ (v. 1.53c) https://imagej.net/ RRID:SCR_003070 Python Programming Language http://www.python.org/ RRID:SCR_008394 OpenCV http://opencv.org RRID:SCR_015526 MatPlotLib http://matplotlib.sourceforge.net RRID:SCR_008624 NumPy http://www.numpy.org RRID:SCR_008633 SimpleITK https://simpleitk.org/ RRID:SCR_024693 (Continued on next page) e2 Neuron 113, 1562–1578.e1–e6, May 21, 2025
Purification:Article Title: Specific targeting of brain endothelial cells using enhancer AAV vectors.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Ethidium Bromide (for maxiprep plasmids) Sigma CAS #: 1239-45-8 Isopropanol (for maxiprep plasmids) Fisher Scientific CAS #: 67-63-0 Phenol (for maxiprep plasmids) Fisher Scientific CAS #: 108-95-2 Chloroform (for maxiprep plasmids) Fisher Scientific CAS #: 67-66-3 Critical commercial assays Pierce Silver Stain Kit (AAV purification) Thermo Fisher Cat#: 24612 Deposited data CATlas http://catlas.org/mousebrain/#! .. RRID:SCR_018690 Mendeley Data: quantification and metadata This paper https://doi.org/10.17632/5kvfknrgng.1 UCSC Genome Browser http://genome.ucsc.edu/ RRID:SCR_005780 Experimental models: Cell lines Cells: AAVpro 293T (for virus packaging) Takara Bio RRID:CVCL_B0XW Experimental models: Organisms/strains Mouse: Tie2-Cre (B6.Cg-Tg(Tek-cre)1Ywa/J) The Jackson Laboratory RRID:IMSR_JAX:008863 Mouse: Ai9 (B6.Cg-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J) The Jackson Laboratory RRID:IMSR_JAX:007909 Mouse: 5xFAD (B6.Cg-Tg (APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax) The Jackson Laboratory RRID:MMRRC_034848-JAX Mouse: B6J (C57BL/6J) The Jackson Laboratory RRID:IMSR_JAX:000664 Oligonucleotides Fwd ITR primer, 50-GGAACCCCTAGTGATGGAGTT Aurnhammer et al.68 PMID: 22428977 Rev ITR primer, 50-CGGCCTCAGTGAGCGA Aurnhammer et al.68 PMID: 22428977 PowerUP SYBR green Master mix Thermo Fisher A46012 Recombinant DNA pUCmini-iCAP-PHP.eB plasmid (encodes engineered PHP.eB capsid protein) Addgene Cat#: 103005; RRID:Addgene_103005 pAAV2/8 (encodes AAV8 capsid protein) Addgene Cat#: 112864; RRID:Addgene_112864 pAAV-BR1 Körbelin et al.13 PMID: 27137490 Ad helper plasmid (mini-pHelper) This paper N/A pAAV-BEC enhancer-minBG-SYFP2 This paper Cat #: 236722; RRID:Addgene_236722 pAAV-minBG-SYFP2 This paper Cat #: 236723; RRID:Addgene_236723 pAAV-CAG-mTurquoise2 Addgene Cat#: 99122; RRID:Addgene_99122 pAAV-BEC enhancer-minBG-iCre This paper Cat #: 236724; RRID:Addgene_236724 pAAV-BEC enhancer-minBG-iCre(R279T)_4x6T This paper Cat #: 236727; RRID:Addgene_236727 P3-Alb-mScarlet Addgene Cat#: 183461; RRID:Addgene_183461 AiP14187 - pAAV-AiE0459m_3xC2-minBGiCre(R297T)-BGHpA (plasmid used to clone out attenuated iCre(R279T)) Addgene Cat#: 229920; RRID:Addgene_229920 AAV-GfaABC1D-ERCreER-4x6T (plasmid used to clone out 4x6T) Addgene Cat#: 196411; RRID:Addgene_196411 Software and algorithms ImageJ (v. 1.53c) https://imagej.net/ RRID:SCR_003070 Python Programming Language http://www.python.org/ RRID:SCR_008394 OpenCV http://opencv.org RRID:SCR_015526 MatPlotLib http://matplotlib.sourceforge.net RRID:SCR_008624 NumPy http://www.numpy.org RRID:SCR_008633 SimpleITK https://simpleitk.org/ RRID:SCR_024693 (Continued on next page) e2 Neuron 113, 1562–1578.e1–e6, May 21, 2025
|
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 