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n o bis  (Thermo Fisher)


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    Structured Review

    Thermo Fisher n o bis
    N O Bis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/043928%2E06/N%2CO-Bis(trimethylsilyl)trifluoroacetamide%2C+with+1%25+TMCS%2C+packaged+under+Argon+in+resealable+ChemSeal+bottles/pmc12938520-76-14-20
    Average 94 stars, based on 1 article reviews
    n o bis - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Paired metabolomics and volatilomics provides insight into transient high light stress response mechanisms of the coral Montipora mollis
    Article Snippet: Metabolite samples were processed and derivatised online via GC-MS by Metabolomics Australia (University of Melbourne) using a Shimadzu AOC6000 autosampler, 2030 Shimadzu gas chromatograph (GC) and a TQ8040 triple-quadrupole mass spectrometer (MS; Shimadzu, Japan). .. Dried-down samples were reconstituted in 25 μL of Methoxyamine Hydrochloride (30 mg mL − 1 in Pyridine), then shaken at 37 °C for 2 h. A 25 μL aliquot of N , O - bis (Trimethylsilyl)trifluoroacetamide with Trimethylchlorosilane (BSTFA with 1% TMCS, Thermo Scientific) was then added to each sample, and incubated for 1 h at 37 °C. .. 1 μL of sample was injected onto the GC column (30 m Agilent DB-5 column with 1 μm film thickness and 0.25 mm internal diameter column) using a hot needle technique, with a 1:10 split.

    Article Title: Seed priming with Streptomyces griseoviridis culture filtrate: species-specific enhancement of legume germination and seedling vigor through hormonal and metabolic signaling.
    Article Snippet: .. For analyses of CFF metabolites, an additional derivatization procedure was employed: dried samples (5 mg) were reacted with 100 μL of a derivatization reagent consisting of N, O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS) (80:20, v/v), followed by incubation at 65 °C for 1 h. Derivatized samples were analyzed using a GC-TSQ mass spectrometer (Thermo Fisher Scientific, Austin, TX, USA) fitted with a TG– 5MS capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness). ..

    Article Title: Serum metabolic fingerprinting of pre-lameness dairy cows by GC-MS reveals typical profiles that can identify susceptible cows.
    Article Snippet: .. Extracted residues were reconstituted with 40 μL methoxyamine hydrochloride (20 mg mL-1; Sigma-Aldrich, Oakvile, ON, Canada) in ACS grade pyridine and incubated at room temperature for 16 h. Then 50 μL of MSTFA (N-Methyl-N-trifluoroacetamide) with 1% TMCS (trimethylchlorosilane) derivatization agent (Thermo Fisher Scientific, Pierce Biotechnology, Rockford, IL, USA) was added and incubated at 80 °C for 1.5 to 2 h on a hotplate. ..

    Article Title: PPT1-mediated plastidic phosphoenolpyruvate import enhances fatty acid biosynthesis in sugar-rich tissues.
    Article Snippet: .. Subsequently, 100 ll of each sample was dried under vacuum and derivatized with 50 ll methoxyamine hydrochloride (SigmaAldrich) (40 mg/ml in pyridine) for 60 min at 50°C, then with 50 ll MSTFA + 1% TMCS (Thermo, MA, USA) at 70°C for 120 min, following a 2-h incubation at room temperature. .. Chromatograms were acquired using a GC-MS system (Agilent Inc.) consisting of an Agilent 7890 gas chromatograph, an Agilent 5975 MSD and a HP 7683B autosampler.

    Article Title: Functions of serine from the phosphorylated pathway on growth, male gametogenesis, and metabolism in Marchantia polymorpha
    Article Snippet: .. After vortexing, the solvents in the tubes were evaporated using a rotary 688 evaporator (Thermo Scientific, Savant, SPD121P, & UVS800DDA) for 3 h, following which 689 100 μL MOX reagent (2% methoxyamine·HCl in pyridine) was added to tubes to dissolve 690 the samples at 30 °C and 1,200 rpm for 6 h. Subsequently, 50 μL MSTFA 1% TMCS 691 (Thermo) was added to the tubes and incubated at 37 °C and 1200 rpm for 30 min. After 692 centrifugation at 5,000 rpm for 1 min, 50 μL supernatant was collected and analyzed using 693 GC-QqQ-MS (AOC-5000 Plus with GCMS-TQ8040, Shimadzu Corporation). ..

    Derivatization:

    Article Title: Seed priming with Streptomyces griseoviridis culture filtrate: species-specific enhancement of legume germination and seedling vigor through hormonal and metabolic signaling.
    Article Snippet: .. For analyses of CFF metabolites, an additional derivatization procedure was employed: dried samples (5 mg) were reacted with 100 μL of a derivatization reagent consisting of N, O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS) (80:20, v/v), followed by incubation at 65 °C for 1 h. Derivatized samples were analyzed using a GC-TSQ mass spectrometer (Thermo Fisher Scientific, Austin, TX, USA) fitted with a TG– 5MS capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness). ..

    Mass Spectrometry:

    Article Title: Seed priming with Streptomyces griseoviridis culture filtrate: species-specific enhancement of legume germination and seedling vigor through hormonal and metabolic signaling.
    Article Snippet: .. For analyses of CFF metabolites, an additional derivatization procedure was employed: dried samples (5 mg) were reacted with 100 μL of a derivatization reagent consisting of N, O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS) (80:20, v/v), followed by incubation at 65 °C for 1 h. Derivatized samples were analyzed using a GC-TSQ mass spectrometer (Thermo Fisher Scientific, Austin, TX, USA) fitted with a TG– 5MS capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness). ..

    Lyophilization:

    Article Title: Individual and interactive effects of temperature and light intensity on canola growth, physiological characteristics and methane emissions.
    Article Snippet: Earlier studies have shown that plants produce methane (CH4) under aerobic conditions, and that this emission is not microbial in nature.. However, the precursors of aerobic CH4 remain under debate, and the combined effects of environmental factors on plant-derived CH4 requires further attention.. The objective of this study was to determine the interactive effects of temperature and light intensity on CH4 and other relevant plant parameters in canola (Brassica napus L.).

    other:

    Article Title: Integrative Analysis of 4-Hydroxynonenal-Modified Proteins and Plasma Metabolome in Breast Cancer Patients
    Article Snippet: All solvents and reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA), except for N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) containing 1% trimethylchlorosilane (TMCS; Pierce Chemical Co., Rockford, IL, USA), the fatty acid methyl ester (FAME) standard mixture (Supelco, Bellefonte, PA, USA), and the API-TOF reference mass solution kit (Agilent Technologies, Waldbronn, Germany).

    Centrifugation:

    Article Title: Functions of serine from the phosphorylated pathway on growth, male gametogenesis, and metabolism in Marchantia polymorpha
    Article Snippet: .. After vortexing, the solvents in the tubes were evaporated using a rotary 688 evaporator (Thermo Scientific, Savant, SPD121P, & UVS800DDA) for 3 h, following which 689 100 μL MOX reagent (2% methoxyamine·HCl in pyridine) was added to tubes to dissolve 690 the samples at 30 °C and 1,200 rpm for 6 h. Subsequently, 50 μL MSTFA 1% TMCS 691 (Thermo) was added to the tubes and incubated at 37 °C and 1200 rpm for 30 min. After 692 centrifugation at 5,000 rpm for 1 min, 50 μL supernatant was collected and analyzed using 693 GC-QqQ-MS (AOC-5000 Plus with GCMS-TQ8040, Shimadzu Corporation). ..



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