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    Structured Review

    Chem Impex International polymer
    Polymer, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/04309/N-Trityl-trimethylenediamine%2C+polymer+bound/10__1021_slash_acs__jmedchem__0c00016-124-10-12
    Average 95 stars, based on 1 article reviews
    polymer - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: pH-Dependent Grafting of Cancer Cells with Antigenic Epitopes Promotes Selective Antibody-Mediated Cytotoxicity
    Article Snippet: Briefly, 5(6)-carboxyfluorescein (2 equiv, Chem-Impex #00472) was coupled onto N-Trityltrimethylenediamine, polymer bound (Chem-Impex #04309) resin with HBTU (2 equiv) and DIEA (4 equiv) at RT for 2 h and then cleaved from resin under the conditions mentioned above.

    Polymer:

    Article Title: pH-Dependent Grafting of Cancer Cells with Antigenic Epitopes Promotes Selective Antibody-Mediated Cytotoxicity
    Article Snippet: To prepare FITC-based conjugates ( 4–9 ), fluorescein 5-maleimide (Invitrogen F150) in DMF was conjugated to an N-terminal cysteine residue of each pHLIP variant ( Scheme S2 ). .. Briefly, 5(6)-carboxyfluorescein (2 equiv, Chem-Impex catalog no. 00472) was coupled to N -trityl-trimethylenediamine, polymer-bound (Chem-Impex catalog no. 04309) resin with HBTU (2 equiv) and DIEA (4 equiv) at RT for 2 h and then cleaved from resin under the conditions mentioned above. .. The amine-modified FITC (5 equiv) was conjugated to polyethylene glycol linkers (PEG 6 , BroadPharm catalog no. 22158; PEG 12 , BroadPharm catalog no. 22217; PEG 24 , Broad-Pharm catalog no. 22218) modified with N -hydroxysuccinimide (NHS) and maleimide with DIEA (5 equiv) in DMF at RT for 3–24 h. Following purification, the resulting FITC-PEG-maleimide linkers in DMF were conjugated to an N-terminal cysteine residue of pHLIP solubilized in 50 mM HEPES (pH 7.4) at RT for 4 h. Peptides were purified via reverse-phase high-performance liquid chromatography (RP-HPLC; Phenomenex Luna Omega, 5 μ m, 250 mm × 21.2 mm C18; flow rate of 5 mL/min; phase A being water with 0.1% TFA; phase B being acetonitrile with 0.1% TFA; 60 min gradient from 95:5 to 0:100 A:B).

    Article Title: pH-Dependent Grafting of Cancer Cells with Antigenic Epitopes Promotes Selective Antibody-Mediated Cytotoxicity.
    Article Snippet: .. Briefly, 5(6)- carboxyfluorescein (2 equiv, Chem-Impex catalog no. 00472) was coupled to N-trityltrimethylenediamine, polymer-bound (Chem-Impex catalog no. 04309) resin with HBTU (2 equiv) and DIEA (4 equiv) at RT for 2 h and then cleaved from resin under the conditions mentioned above. .. The amine-modified FITC (5 equiv) was conjugated to polyethylene glycol linkers (PEG6, BroadPharm catalog no. 22158; PEG12, BroadPharm catalog no. 22217; PEG24, Broad-Pharm catalog no. 22218) modified with N-hydroxysuccinimide (NHS) and maleimide with DIEA (5 equiv) in DMF at RT for 3–24 h. Following purification, the resulting FITC-PEG-maleimide linkers in DMF were conjugated to an Nterminal cysteine residue of pHLIP solubilized in 50 mM HEPES (pH 7.4) at RT for 4 h. Peptides were purified via reverse-phase high-performance liquid chromatography (RPHPLC; Phenomenex Luna Omega, 5 μm, 250 mm × 21.2 mm C18; flow rate of 5 mL/min; phase A being water with 0.1% TFA; phase B being acetonitrile with 0.1% TFA; 60 min gradient from 95:5 to 0:100 A:B).



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